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쌍태중 정상아와 동반된 내장탈출 및 2분 척추의 무뇌아1례
최정숙(JS Choi),정현택(HT Chung) 대한산부인과학회 1977 Obstetrics & Gynecology Science Vol.20 No.2
A case that anencephaly associated with visceral eventration, spina bifida with meningom- yelocele, cleft lip with cleft palate, dysplasia of rib cage and dysplasia of extremities skeleton of single ovum twin is presented with brief review of literature
인간양수를 이용한 2-세포기 생쥐배아의 체외발달에 관한 연구
김두호,조인제,승경록,정길생,정형민,황성진,이훈택 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.10
인간양수를 이용한 2-세포기 생쥐배아의 체외발달에 관하여 연구를 수행한 결과 다음과 같은 결론을 얻었다. 1. 기본 배양액에 20% 농도로 양수를 첨가한 군이 다른 첨가군에 비하여 다소 높은 배아 발달율을 보였으나 통계상의 유의성은 없었다. 그러나 순수양수만을 사용한 군에서는 배아 발달율이 현저히 낮았으며 통계상의 유의성이 있었다. 2. 소의 양수와는 달리 인간 양수의 반복된 동결용해는 배양성적에 별다른 영향이 없었다. 3. 0.3% 소 혈청알부민 및 20%양수를 각각 첨가한 배양액에서의 배아발달율을 관찰한 실험 Ⅲ에서는 각 군에서 상실배, 포배 및 부화란까지 발달율은 유사하였고 양수 첨가군에서 다소 높았으나 통제상의 유의성은 없었다, 4. 형광형미경하에서 평윤 포배의 세포수를 관찰한 결과 양수 첨가군이 약10%정도 많은 세포수를 보였으나 통계상의 유의성은 없었다. 5. 이상으로 볼 때 인간 양수는 시험관 아기 프로그램에 있어서 배양조건의 정도관리 및 임신성공율의 향상에 있어서 첨가물로서 선택적인 이용 가능성이 있다고 사료된다. To evaluate the effectiveness of human amniotic fluid for culture of 2-cell stage mouse embryos, series of experiemnts was conducted. In experiemnt 1, more embryos hatched in medium containing 20% amniotic fluid althoughno statistical difference was found among other two groups but there was remarkable decline of hatching rate in 100% amniotic fluid. In experiemnt 2 repeated freezing and thawing of amniotic fluid influenced unremarkable effect on the development of 2-cell atage mouse embryos. In experiment 3, suppoementation of each medium with 0.3%bovine serum albumin and 20% amniotic fluid revealed similar effect on embryo development. In experiment 4, improved embryo quality was observed by fluorescent staining about the embryos which were cultured in medium containing amniotic fluid.
서병희,고영호,김재명,정길생,이훈택,손성수 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.5
Mouse GV oocytes enclosed cumulus cell were collected from antral follicles at the GV stage. They were released into maturation demium containing the meiotic inhibitor dbcAMP. They were stored in straws at-196 C after slow and ultrarapid freezing method using DMSO, PROH and Glycerol or a mixture of each agent as a cryoprotectant. We investigated survival rates, in vitro maturation rates, and two cell and blastocyst formation rates after whawing. An optimal cryosurvival rate of 75.2% was attained after ultrarapid freezing with 3M DMSO by one step dilution, but a rate of two cell formation was 26.7%. At the cryosurvival rates after freezing with 1.2-PROH, 1.2-PROH indicated its inappropriateness for ultrarapid freezing except 1.5M PROH by one step dilution group(50.0%). Both two cell and blastocyst formation rates were very low. We reported that cryopreservation of immature GV stage oocytes was invariably associated with a low developmental capacity after fertilization. The rate of in vitro nuclear maturation did not equate with developmental competence. Therefore, wer were suggested that cytoplasmic maturation would be important to embryonic development.
A Uterus Didelphys with Double Pregnancy
김대현(TH Kim),차희국(HK Cha),권혁동(HT Kwon),오문애(MA Oh),정종진(JJ Chung) 대한산부인과학회 1970 Obstetrics & Gynecology Science Vol.13 No.11
This uterus didelphys with double pregnancy(one pregnancy in each uterus) is the first case which has been reported in Korea. Several cases of this kind have been reported in the world. This is probably the first case of double pregnancy in uterus didelphys in which the babies were delivered by Cesarean section in good condition. The literatures have been reviewed on this subject.
김홍균(HG Kim),정창조(CJ Chung),권혁동(HT Kwon),김대현(TH Kim),김종수(JS Kim) 대한산부인과학회 1977 Obstetrics & Gynecology Science Vol.20 No.10
1966년 1월 1일부터 1975년 12월 31일 까지 연세대학교 부속 원주 기독병원 산부인과에서 임신 20주이후에 분만한 총 7,236 예의 출산아를 대상으로 심한 선천성 기형에 대해서 관찰하여 다음과 같은 결론을 얻었다. 1. 총 출산아 중 심한 선천성 기형아의 발생 빈도는 74예로 1.02%이였고 남아가 여아보 다 많은 발생 빈도를 나타냈다. 2. 저 체중아의 심한 선천성 기형아의 발생 빈도는 성숙아 0.92%인데 비해 1.72%로 현저히 높은 발생 빈도를 나타냈다. 3. 산모의 연령과 분만 회수가 많으면 많을수록 선천성 기형아의 발생 빈도는 높은 것 으로 관찰되었다. 4. 신생아에서 심한 선천성 기형아 발생빈도는 0.72%이고 사산아에서 기형아 발생 빈도 는 7.87%로서 사산아에서 심한 기형아 발생 빈도가 높았다. We have observed 74 cases of congenitally malformed babies among 7,236 newborns delivered after gestation of 20 weeks in Won-Ju Christian Hospital affiliated to Yonsei University College of Medicine from Jan. 1, 1966 to Dec, 31, 1975. This is clinical strdy of 74 congenitally malformed babies concerned in the incidence of major congenital anomalies. The results are as follows. 1. Among the total 7,236 newborns, the incicence of major congenitally malformed higher than in matured babies (0.92%) 2. The incidence of congenitally malformed babies in prematurity (1.72%) was higher than in matured babies(0.92%) 3. The incidence of congenitally malformed babies was highre when the number of parity and the age of mother increased. 4. The incidence of congenitally malformed babies in stillbirth was 7.87% as compared with 0.72% in live-birth