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Choi, Dahee,Oh, Kyoung‐,Jin,Han, Hye‐,Sook,Yoon, Young‐,Sil,Jung, Chang‐,Yun,Kim, Seong‐,Tae,Koo, Seung‐,Hoi Wiley Subscription Services, Inc., A Wiley Company 2012 Hepatology Vol.56 No.4
<P><B>Abstract</B></P><P>Postprandial insulin plays a critical role in suppressing hepatic glucose production to maintain euglycemia in mammals. Insulin‐dependent activation of protein kinase B (Akt) regulates this process, in part, by inhibiting FoxO1‐dependent hepatic gluconeogenesis by direct phosphorylation and subsequent cytoplasmic exclusion. Previously, it was demonstrated that protein arginine methyltransferase 1 (PRMT1)‐dependent arginine modification of FoxO1 interferes with Akt‐dependent phosphorylation, both in cancer cells and in the <I>Caenorhabditis elegans</I> model, suggesting that this additional modification of FoxO1 might be critical in its transcriptional activity. In this study, we attempted to directly test the effect of arginine methylation of FoxO1 on hepatic glucose metabolism. The ectopic expression of PRMT1 enhanced messenger RNA levels of FoxO1 target genes in gluconeogenesis, resulting in increased glucose production from primary hepatocytes. Phosphorylation of FoxO1 at serine 253 was reduced with PRMT1 expression, without affecting the serine 473 phosphorylation of Akt. Conversely, knockdown of PRMT1 promoted an inhibition of FoxO1 activity and hepatic gluconeogenesis by enhancing the phosphorylation of FoxO1. In addition, genetic haploinsufficiency of <I>Prmt1</I> reduced hepatic gluconeogenesis and blood‐glucose levels in mouse models, underscoring the importance of this factor in hepatic glucose metabolism <I>in vivo</I>. Finally, we were able to observe an amelioration of the hyperglycemic phenotype of <I>db/db</I> mice with PRMT1 knockdown, showing a potential importance of this protein as a therapeutic target for the treatment of diabetes. <I>Conclusion:</I> Our data strongly suggest that the PRMT1‐dependent regulation of FoxO1 is critical in hepatic glucose metabolism <I>in vivo</I>. (H<SMALL>EPATOLOGY</SMALL> 2012)</P>
Risk Evaluation of Azithromycin-Induced QT Prolongation in Real-World Practice
Choi, Young,Lim, Hong-Seok,Chung, Dahee,Choi, Jung-gu,Yoon, Dukyong Hindawi 2018 BioMed research international Vol.2018 No.-
<P><B>Background</B></P><P> Azithromycin exposure has been reported to increase the risk of QT prolongation and cardiovascular death. However, findings on the association between azithromycin and cardiovascular death are controversial, and azithromycin is still used in actual practice. Additionally, quantitative assessments of risk have not been performed, including the risk of QT prolongation when patients are exposed to azithromycin in a real-world clinical setting. Therefore, in this study, we aimed to evaluate the risk of exposure to azithromycin on QT prolongation in a real-world clinical setting using a 21-year medical history database of a tertiary medical institution.</P><P><B> Methods</B></P><P> We analyzed the electrocardiogram results and relevant electronic health records of 402,607 subjects in a tertiary teaching hospital in Korea from 1996 to 2015. To evaluate the risk of QT prolongation of azithromycin, we conducted a case-control analysis using amoxicillin for comparison. Multiple logistic regression analysis was performed to correct for age, sex, accompanying drugs, and disease.</P><P><B> Results</B></P><P> The odds ratio (OR) for QT prolongation (QTc>450 ms in male and >460 ms in female) on azithromycin exposure was 1.40 (95% confidence interval [CI], 1.23-1.59), and the OR for severe QT prolongation (QTc>500 ms) was 1.43 (95% CI, 1.13-1.82). On the other hand, the ORs on exposure to amoxicillin were 1.06 (95% CI, 0.97-1.15) and 0.88 (95% CI, 0.70-1.09). In a subgroup analysis, the risk of QT prolongation in patients aged between 60 and 80 years was significantly higher when they are exposed to azithromycin.</P><P><B> Conclusions</B></P><P> The risk of QT prolongation was increased when patients, particularly the elderly aged 60-79 years, were exposed to azithromycin. Therefore, clinicians should pay exercise caution using azithromycin or consider using other antibiotics, such as amoxicillin, instead of azithromycin.</P>
Olfactory receptor 544 reduces adiposity by steering fuel preference toward fats
Wu, Chunyan,Hwang, Su Hyeon,Jia, Yaoyao,Choi, Joobong,Kim, Yeon-Ji,Choi, Dahee,Pathiraja, Duleepa,Choi, In-Geol,Koo, Seung-Hoi,Lee, Sung-Joon American Society for Clinical Investigation 2017 The Journal of clinical investigation Vol.127 No.11
Choi, Eunji,Oh, Jungju,Lee, Dahee,Lee, Jaewon,Tan, Xiaonan,Kim, Minkyung,Kim, Gyeungyun,Piao, Chunxian,Lee, Minhyung Elsevier 2018 Journal of controlled release Vol.279 No.-
<P><B>Abstract</B></P> <P>The receptor for advanced glycation end-products (RAGE) is involved in tumor angiogenesis. Inhibition of RAGE might be an effective anti-angiogenic therapy for cancer. In this study, a cationic RAGE-binding peptide (RBP) was produced as an antagonist of RAGE, and a ternary-complex consisting of RBP, polyethylenimine (2 kDa, PEI2k), and a suicide gene (pHSVtk) was developed as a gene delivery system with dual functions: the anti-tumor effect of pHSVtk and anti-angiogenic effect of RBP. As an antagonist of RAGE, RBP decreased the secretion of vascular-endothelial growth factor (VEGF) in activated macrophages and reduced the tube-formation of endothelial cells <I>in vitro</I>. In <I>in vitro</I> transfection assays, the RBP/PEI2k/plasmid DNA (pDNA) ternary-complex had higher transfection efficiency than the PEI2k/pDNA binary-complex. In an intracranial glioblastoma animal model, the RBP/PEI2k/pHSVtk ternary-complex reduced α-smooth muscle actin expression, suggesting that the complex has an anti-angiogenic effect. In addition, the ternary-complex had higher pHSVtk delivery efficiency than the PEI2k/pHSVtk and PEI25k/pHSVtk binary-complexes in an animal model. As a result, the ternary-complex induced apoptosis and reduced tumor volume more effectively than the PEI2k/pHSVtk and PEI25k/pHSVtk binary-complexes. In conclusion, due to its dual anti-tumor and anti-angiogenesis effects, the RBP/PEI2k/pHSVtk ternary-complex might be an efficient gene delivery system for the treatment of glioblastoma.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Dahee Yoon ),( Jaeuk Jin ),( Wuon-shik Kim ),( Sun-mi Choi ),( Jaeuk Kim ) 한국감성과학회 2023 한국감성과학회 국제학술대회(ICES) Vol.2023 No.-
This document explored the neural mechanisms underpinning motor execution (ME) and motor imagery (MI), focusing on the motor cortex’s critical regions, C3 and C4. These areas are instrumental in coordinating movements, adhering to a contralateral organization. To investigate these processes, we employed functional nearinfrared spectroscopy (fNIRS) during ME and MI. Our experimental results obtained through fNIRS revealed contralateral patterns of brain activation, illustrating contralateral activation in the absence of actual movement.