Integration for Heterogeneous Manufacturing Information Systems Based on Semantic Entity

Song Yu-chuan,Xiong Jun,LI Xian-wang,Chen Xue-hai3 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.9

To support semantic integration for heterogeneous information systems in manufacturing enterprises, based on semantic gateway, the concept of semantic entity is defined, and the semantic entity-based integration method is studied. Semantic entity sets up conceptual structure by aggregating semantic concept from semantic gateway, and builds dynamic conceptual structure through adding or removing its general concept. By means of key business data, semantic entity identifies business object and obtains business data from its application system, and then instantiates concept structure. Based on instantiation, semantic service can obtain parameters data from business object instant, and satisfies the interface contract. Semantic entity captures data changes of business object, and then establishes status-based integration rules, defines algorithm logic of rules. By instantiating concept structure aimed to specific application system and invoking semantic service, the integration activities among information systems come true. This work has been verified by prototype system, and attained expected integration effect.

Robust Finite-time Attitude Tracking Control of Rigid Spacecraft Under Actuator Saturation

Hai-Tao Chen,Shen-Min Song,Zhi-Bin Zhu 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.1

This paper investigates the robust finite-time attitude tracking control problem for rigid spacecraft considering the modeling uncertainty, external disturbance and actuator saturation. An auxiliary system is proposed to directly compensate for the saturated control input. First, the basic controller is formulated based on the fast nonsingular terminal sliding mode surface (FNTSMS), the fast-TSM-type reaching law and the auxiliary system in the presence of upper bounded external disturbance. Then, when facing system uncertainty which consists of both modeling uncertainty and external disturbance and has upper bounded first derivative, the extended state observer (ESO) is associated with the first controller to improve the robustness of control system. Furthermore, to handle more general system uncertainty which is upper bounded by a polynomial function of the closed-loop system states, a continuous adaptive controller is designed to compensate for the total system uncertainty on line. The proposed controllers are able to deal with system uncertainty, input singularity and actuator saturation, while simultaneously providing fast finite-time convergence speed for the control system. And the problems of complex parameters selection process and repeated differentiations of nonlinear functions can be avoided. Rigorous stability analyses are given via the Lyapunov stability theory and digital simulations are conducted to illustrate the effectiveness of the proposed controllers.

Multiple Impairments in Male Reproduction 1 (mimr1), a Novel Male-Sterile Mutant of Arabidopsis thaliana, Shows Several Defects in Male Reproductive Development

Hai-Yan Chen,Yue-Feng Guan,Xue-Yong Huang,Yu-Ting Wu,Fen-Fei Wang,Ju-Fang Gao,Que Zhou,Zhong-Nan Yang,Jia-Yao Liu,Hong-Xia Zhang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3

We have characterized a new male-sterile mutant in Arabidopsis that exhibits conditional sterility but has restored fertility when drought-stressed. This mutant,multiple impairments in male reproduction 1 (mimr1),shows pleiotropic defects in both vegetative and reproductive development. Examination with dissecting and scanning electron microscopes revealed that its pollen grains are not effectively released from the anther locule after dehiscence, and anther differentiation is defective. Growth of the style and stamen filaments are also abnormal. Histological analysis demonstrated that these phenomena are due not only to a noticeably reduced extension of the stamen but also greater elongation of the pistil. Genetic analysis indicated that mimr1 is a single locus recessive nuclear mutant. The mutation can be mapped to a locus strongly linked to a 1200-kb region on Chromosome 3. Meta-analysis of expression patterning presented several candidate genes in that region. No mutants with similar phenotypes have previously been reported, suggesting that mimr1 is a novel male-sterile locus. Characterization of MIMR1 will provide further insights into the molecular basis for the development of plant reproductive organs.

Transcriptome-wide identification of miRNA targets and a TAS3- homologous gene in Populus by degradome sequencing

Hai Bao,Min Chen,Hui Chen,Liang Du,Yanwei Wang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Degradome sequencing has been applied to identify miRNA-directed mRNA cleavage and understand the biological function of miRNAs and their target genes in plants defense to stress. miRNAs involved in the response to cold stress have been identified in Populus, however, there are few reports about the validated targets of miRNAs in Populus under cold stress. Objectives The primary objective of this investigation was to globally identify and validate the targets of the miRNAs and regulatory components in Populus under cold stress. Methods Populus plantlets grown in vitro were treated with cold (4 °C for 8 h) and total RNA was extracted using Trizol reagent. Approximately 200 μg total RNA was used for the construction of the degradome library, and degradome sequencing was conducted on an Illumina HiSeq 2000. The sequences were mapped to Populus genome using SOAP 2.0 and then were collected for degradome analysis. Additionally, trans-acting siRNA sequences from transacting siRNA gene 3 sequences and mature miRNAs cleaved from precursor miRNAs of Populus were analyzed. 5′ RNA ligase-mediated-RACE (5′-RACE) were further conducted. Results 80 genes were experimentally determined to be the target of 51 unique miRNAs, including three down-regulated miRNAs (pto-miR156k, pto-miR169i-m, and pto-miR394a-5p/b-5p) and two up-regulated miRNAs (pto-miR167a-d and pto-miR167f/g). The specificity and diversity of cleavage sites of miRNA targets were validated through 5′-RACE experiment and the results were similar with that of degradome sequencing, further supporting the empirical cleavage of miRNAs on targets in vivo in Populus. Interestingly, the TAS-homologous gene pto-TAS3 (EF146176.1) was identified and 11 potential ta-siRNAs [D1(+)–D11(+)] and their possible biogenesis sites within the pto-TAS3 transcript sequence were predicted in Populus. In addition, the biosynthesis of miRNA from precursor miRNA (pre-miRNA) was also validated through the detection of a total of 17 pre-miRNAs. Conclusion Our investigation expands the application of degradome sequencing for evaluating miRNA regulatory elements and evidence of the miRNA synthesis process, and provides empirical evidence of bona fide cleavage of target genes by miRNAs in Populus, which might be used for the research of miRNA-mediated regulation mechanism and molecular improvement of resistance to cold stress.

Automatic balancing of a flexible supported rotor with two disks by two ball balancers

Hai-Wei Chen,Yan-Bin Chen,Zhen Sun,Zhi Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.7

This paper studies automatic balancing of a flexible supported rotor with two disks by two ball balancers. Firstly, reduced dynamic descriptions of different components in the rotor are derived using a special method according to Lagrangian equation, based on which a general vibration model of the rotor system is then constructed. Secondly, equations governing frequency responses of the rotor are derived and working principles of the balls in the rotor are illustrated based on basic dynamic phenomena. Thirdly, an autonomous model of the rotor system is built with equations governing movement of the balls derived. Three equilibrium solutions are discovered whose dynamic characteristics are then studied using numerical bifurcation analysis method. Finally, locations of the balls at the steady state are observed using a high speed camera, and the capability of the two balancers for automatic balancing under different conditions is validated.

Investigation of the SHM-oriented model and dynamic characteristics of a super-tall building

Hai-Bei Xiong,Ji-Xing Cao,Feng-Liang Zhang,Xiang Ou,Chen-Jie Chen 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.23 No.3

Shanghai Tower is a 632-meter super high-rise building located in an area with wind and active earthquake. A sophisticated structural health monitoring (SHM) system consisting of more than 400 sensors has been built to carry out a long-term monitoring for its operational safety. In this paper, a reduced-order model including 31 elements was generated from a full model of this super tall building. An iterative regularized matrix method was proposed to tune the system parameters, making the dynamic characteristic of the reduced-order model be consistent with those in the full model. The updating reduced-order model can be regarded as a benchmark model for further analysis. A long-term monitoring for structural dynamic characteristics of Shanghai Tower under different construction stages was also investigated. The identified results, including natural frequency and damping ratio, were discussed. Based on the data collected from the SHM system, the dynamic characteristics of the whole structure was investigated. Compared with the result of the finite element model, a good agreement can be observed. The result provides a valuable reference for examining the evolution of future dynamic characteristics of this super tall building.

Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus

Chen-Chen Zhao,Yang Yang,Hai-tao Wu,Zhi-Mo Zhu,Yue Tang,Cui-Ping Yu,Na Sun,Qiang Lv,Jia-Run Han,Ao-Ting Li,Jia-Nan Yan,Yue Cha 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4- guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50oC and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis.

Water and carbohydrate levels at different developmental stages and dynamics in hibernating pupae of Pieris melete (Lepidoptera: Pieridae)

Hai-Jun XIAO,JunHui CHEN,Chao CHEN,Fang-Sen XUE 한국곤충학회 2015 Entomological Research Vol.45 No.5

For insight into the physiological indicators of diapause in Pieris melete, water and carbohydrate (glycogen and trehalose) levels were measured under both natural and laboratory conditions. The highest water content (3.71–3.79 mg/mg dry weight) was found in larvae and developing pupae, which was substantially higher than in diapausing pupae (2.59 mg/mg dry weight). Water content was almost stable during diapause, except for individuals approaching diapause termination (3.43–3.58 mg/mg dry weight). The total carbohydrate level was significantly higher in pre‐pupae (47.41 μg/mg) compared to larvae (22.80 μg/mg) and developing pupae (21.48 μg/mg). The highest level of trehalose was detected in winter diapausing pupae, and no trehalose was found in larvae or developing pupae. Levels of glycogen were highest in pre‐pupae and lowest in diapausing pupae. Levels of total carbohydrate decreased as diapause proceeded, and no significant changes were found in trehalose levels for diapausing pupae under natural conditions or treated for 60–90 days at 5°C. Pupae treated at 20°C for 60–90 days had significantly lower levels of trehalose than those treated for 30 days. Glycogen content was relatively stable at 5°C, but increased after treatment under natural conditions and 20°C for more than 60 days. These results suggest that the dynamics of water and carbohydrate levels are potential physiological diapause indicators, which show metabolic differences between trehalose and glycogen during diapause development.

An Investigation of Surface Roughness in Ultrasonic Assisted Dry Grinding of 12Cr2Ni4A with Large Diameter Grinding Wheel

Hai-Feng Chen,Jin-Yuan Tang,Wen Shao,Bo Zhao 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.6

Ultrasonic assisted dry grinding (UADG) is a novel green manufacturing technology for decreasing the negative environment impact of cutting fluids and improving the surface characteristics. In this study, the influences of the ultrasonic amplitude, grinding depth and grinding velocity on the surface roughness in ultrasonic assisted dry grinding of 12Cr2Ni4A with a large CBN grinding wheel were investigated. Due to the Poisson effect, the ultrasonic assisted dry grinding using a large diameter grinding wheel is the combination of axial ultrasonic assisted grinding and radial ultrasonic assisted grinding. The results indicated that the main axial ultrasonic component tended to smooth the surface topography by increasing the interaction overlap of the adjacent cutting traces, but it would result in more side flow/ploughing on the surface at a larger ultrasonic amplitude; the radial ultrasonic component exerted a function on the increase of the surface roughness through deepening the individual grinding trajectories. Thus, the surface roughness decreased first and then increased with the increase of grinding depth due to the combined contribution of axial and radial vibrations. However, the improving effect of ultrasonic vibration on the surface roughness gradually weakened with the increase of grinding velocity. Under proper operating parameters, surface roughness obtained in ultrasonic assisted dry grinding can be reduced up to 30% compared with that of common dry grinding.

Expression Analysis of miRNAs in Porcine Fetal Skeletal Muscle on Days 65 and 90 of Gestation

Chen, Jian-hai,Wei, Wen-Juan,Xiao, Xiao,Zhu, Meng-Jin,Fan, Bin,Zhao, Shu-Hong Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.7

MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.