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Effect of Metabolic Structures and EnergyRequirements on Curdlan Production byAlcaligenes faecalis
Xiao Bei Zhan,Zhi-Yong Zheng,Jin Woo Lee,Zhongping Shi,Lei Wang,Li Zhu,Jian-Rong Wu,Chi Chung Lin 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
A comprehensive metabolic network was proposed for Alcaligenes faecalis and employed in a stoichiometrically based flux balance model for curdlan production optimization. The maximal yield of curdlan was evaluated for curdlan batch production. Various metabolic structures and metabolic pathway distributions related with the curdlan maximal yield was evaluated. The results showed that the energy efficiency rather than the substrate supply was the major constraint for the enhancement of curdlan production. The increase in specific rate of glucose uptake could enhance curdlan production yield due to the decrease of the ratio of metabolic maintenance to substrate consumption. However, some of the energy loss and nutrient limitation associated with the increase of metabolic maintenance would adversely affect the conversion efficiency of the substrate.
Hua Gu,Lei Huang,Bei Xi,QiuLi Qin 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.4
At present, the distribution of China’s medical resources is uneven. Every day big hospitals are overwhelmed. The problem that it’s difficult and expensive for ordinary people to see doctors is becoming a major social host issue. And major chronic diseases are threatening human health. In order to optimize medical resource allocation and provide more efficient services for patients with major chronic diseases, this paper takes stroke as the entry point and proposes a new medical service pattern. Namely, taking stroke patients as the center, medical association collaborates to prevent and treat them. Because cloud computing is dynamic and scalable and in order to expand to other major diseases and attract more medical associations to join when the medical service pattern is mature, this paper uses cloud computing to support the pattern. And this paper details the new medical service pattern, the framework and the service of cloud platform of medical association collaborating to prevent and treat stroke. The pattern can consider the prevention, treatment, rehabilitation, and other aspects of stroke. And it can achieve the circular medical service pattern which centered on stroke patients and can provide better service for stroke patients.
Li-Ming Sun,Bei-Wei Zhu,Hai-tao Wu,Lei Yu,Da-Yong Zhou,Xiuping Dong,Jing-Feng Yang,Dong-Mei Li,Wen-Xiu Ye,Yoshiyuki Murata 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4
Cathepsin B from the gut of sea cucumber (Stichopus japonicas) was purified 81-fold with a 3%recovery by ammonium sulfate fractionation and a series chromatography on DEAE Sepharose CL-6B, Sephadex G-75, and TSK-Gel 3000 SWxl. The purified protein appeared as a single band on Native-PAGE but showed 2bands of 23 and 26 kDa on SDS-PAGE. The optimum activity was found at pH 5.5 and 45°C. The enzyme was stable at pH 4.5-6.0 and the thermal stability was up to 50oC. The enzyme was strongly inhibited by E-64, iodoacetic acid, and antipain, demonstrating it is a cysteine protease containing sulfhydryl groups. Cu^2+, Ni^2+, and Zn^2+ could strongly inhibit the enzyme activity. The amino acid sequences of the purified enzyme were acquired by mass spectrometer, which did not show any homology with previously described cathepsins, suggesting it may be a novel member.
Xiang-li Long,Bei-bei Duan,Hai-xia Cao,Ming-lei Jia,Long-an Wu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.62 No.-
The simultaneous scrubbing of NO and SO2 can be finished with the Co(NH3)62+ ammonia solution. Activated carbon aids the regeneration of Co(NH3)62+ to retain the ability of absorbing NO. Acetic acid is tried to improve the catalytic capability of activated carbon. The best treatment condition is the carbon samples impregnated in 2.0 mol l−1 HAc solution for 20 h followed by being calcined at 600 °C for 4 h. The HAc modification increases surface area and acidic groups on the carbon surface. The experiments prove that the modified carbon can obtain a higher NO removal efficiency than the original carbon.
Liu Ziying,Gao Lei,Cheng Long,Lv Gaoyuan,Sun Bei,Wang Gang,Tang Qiushi 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
N6-methyladenosine (m6A) is one of the epigenetic modifications of RNA. The addition of this chemical mark to RNA molecules regulates gene expression by affecting the fate of the RNA molecules. This posttranscriptional RNA modification is reversible and regulated by methyltransferase “writers” and demethylase “erasers”. The fate of m6A-modified RNAs depends on the function of different “readers” that recognize and bind to them. Research on m6A methylation modification has recently increased due to its important role in regulating cancer progression. Noncoding RNAs (ncRNAs) are a class of RNA molecules that are transcribed from the genome but whose roles have been overlooked due to their lack of well-defined potential for translation into proteins or peptides. However, this misconception has now been completely overturned. ncRNAs regulate various diseases, especially tumors, and it has been confirmed that they play either tumor-promoting or tumor-suppressing roles in almost all types of tumors. In this review, we discuss the m6A modification of different types of ncRNA and summarize the mechanisms involved. Finally, we discuss the progress of research on clinical treatment and discuss the important significance of the m6A modification of ncRNAs in the clinical treatment of tumors.
Study on the Internal Pipeline Inspection Devices with Energy Acquisition Methods
Wang Ming-Xu,차인수,Wang Yue,Bei Lei,최홍준,정경환,김동묵,김봄,오승아,Ye In Cheol,유일준 한국에너지학회 2023 에너지공학 Vol.32 No.1
The pipeline conveying natural gas must be regularly inspected by the detection device, and the cracks and corrosion condition inside the pipeline should be detected. Generally, the detection device consists of three parts: drive part, detection part and energy part. Since pipeline transportation is long-distance transportation and cannot realize wired detection, the energy part needs to provide enough electric energy for the detection part to meet the energy consumption of the detection part. The method of obtaining energy for the energy part is very important. In this paper, the energy part is studied, and an efficient energy part is designed. By analyzing the change law of the positive pressure on the inner wall of the energy acquisition wheel on the pipeline, the corresponding torque and power changes are obtained when the detector moves at different speeds. Through the test platform system, the output power of the energy part at different speeds is collected, and the accuracy of the theoretical calculation is verified.
Effect of Metabolic Structures and Energy Requirements on Curdlan Production by Alcaligenes faecalis
Zheng, Zhi-Yong,Lee, Jin-Woo,Zhan, Xiao Bei,Shi, Zhongping,Wang, Lei,Zhu, Li,Wu, Jian-Rong,Lin, Chi Chung Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
A comprehensive metabolic network was proposed for Alcaligenes faecalis and employed in a stoichiometrically based flux balance model for curdlan production optimization. The maximal yield of curdlan was evaluated for curdlan batch production. Various metabolic structures and metabolic pathway distributions related with the curdlan maximal yield was evaluated. The results showed that the energy efficiency rather than the substrate supply was the major constraint for the enhancement of curdlan production. The increase in specific rate of glucose uptake could enhance curdlan production yield due to the decrease of the ratio of metabolic maintenance to substrate consumption. However, some of the energy loss and nutrient limitation associated with the increase of metabolic maintenance would adversely affect the conversion efficiency of the substrate.