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유산균들의 콜레스테를 저하성, 내산성, 내담즙성, 항생제 내성 비교
박소영,고영태,정후길,양진오,정현서,김영배,지근억 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3
기능성 요구르트의 제조에 사용되는 유산균은 인체에 유익한 생리활성과 우수한 생존능력을 보유하고 있는 것이 바람직하다. 본 연구에서는 다양한 유산균주의 산과 담즙 및 항생제에 대한 내성을 조사하였으며 in vitro에서 콜레스테롤 저하능을 조사하였다. In vitro에서 콜레스테롤 저하능이 우수한 13균주를 선발하여 이들의 동결건조 분말을 고 콜레스테롤 식이의 실험쥐에 투여하였다. 그 결과 8균주는 비 투여구보다 유의적으로 18.3~27.3%의 콜레스테롤 저하능을 나타냈다(P≤0.05). 이들 중에서 Bifidobacterium infantis AM-220, Lactobacillus AM-245, Streptococcus MA-1의 3균주를 이용하여 요구르트를 제조한 후 동결건조 분말을 이용하여 급여실험을 수행한 결과, 총 콜레스테롤과 LDL 함량이 유의적으로 감소하는 것으로 나타났다. For a probiotic yoghurt it is desirable to utilize lactic acid bacteria with a high survival rate and beneficial function to human beings. We have examined a variety of lactic acid bacteria to assess the acid and bile tolerance and antibiotic resistance. In addition, an in vitro culture experiment was performed to evaluate their ability to reduce cholesterol levels in the growth medium. Thirteen strains were selected from in vitro cholesterol assays and fed to Sprague-Dawley rats with a high-cholesterol diet. Among the 13 strains tested, 8 strains were shown to reduce serum cholesterol levels significantly after 24 days of administration in vivo. Rats were fed lyophilized yoghurt powder fermented with a combination of 3 selected strains: Bifidobacterium infantis AM-220, Lactobacillus AM-245, and Streptococcus MA-1. The levels of total cholesterol and low density lipoprotein were significantly lower (p≤0.05) in rats fed the yoghurt powder compared with control group. These studies suggest that yoghurt fermented with appropriately selected lactic acid bacteria may have a anticholesterolemic effect.
金鍾一,朴永錫,丁梧鎭,李秀一,金熙峰,裵相龍,秋相根 조선대학교 동력자원연구소 1986 動力資源硏究所誌 Vol.8 No.2
This research was undertaken to study the mineralogy, and the optimum conditions for uranium leaching and extraction from the Okchon system graphite ores in the Kumsan areas. A research has been needed to study the refining process for the effective leaching and extraction from uranium ores in the Kumsan area which has more uranium deposits than any other area. By the mineralogical study with the powder X-ray diffraction method, primary uranium minerals are uraninite, brannerite, coffinite etc. and secondary uranium minerals are uranocircite, niobium uranium oxide, carnotite, toberite etc. has developed in the studied area uranium ores. The volumetric ratio of the primary and the secondary uranium minerals are approximatly 1 : 5. In this experiment, the uranium-bearing graphite in the Kumsan area was milled with a jaw crusher and ballmill. This milled uranium-bearing graphite was leached by sulfuric acid and from this sulfuric acid-leaching solution, uranium was extracted by Amberlite LA-1 in kerosene. The results are as follows: (1) The optimum results in the leaching process are : acidity pH = 1.0, temperature : 25.0℃, particle size : -80+120 mesh, leaching time : 24 hours, pulp density : 50% solid (2) A good pH range for uranium extraction was between 0.8 and 1.3. (3) The uranium distribution coefficient increases in direct proportional to the concentration of the Amberlite LA-1 as extractive agent. (4) The optimum time at 200rpm was 15 minutes.
Primary Extraskeletal Mesenchymal Chondrosarcoma Arising from the Pancreas
Bae-Geun Oh,Yoon Hee Han,Byung Hoon Lee,Su Young Kim,Yoon Joon Hwang,Jung Wook Seo,Yong Hoon Kim,차순주,Gham Hur,Mee Joo 대한영상의학회 2007 Korean Journal of Radiology Vol.8 No.6
We report here on a case of primary extraskeletal mesenchymal chondrosarcoma that arose from the pancreas. A 41-year-old man was evaluated by CT to find the cause of his abdominal pain. The CT scans showed a heterogeneously enhancing necrotic mass with numerous areas of coarse calcification, and this was located in the left side of the retroperitoneal space and involved the body and tail of the pancreas. Portal venography via the celiac axis also showed invasion of the splenic vein. Following excision of the mass, it was pathologically confirmed to be primary extraskeletal mesenchymal chondrosarcoma that arose from the pancreas.
Lee, Geun-Shik,Jung, Eui-Man,Choi, Kyung-Chul,Oh, Goo Taeg,Jeung, Eui-Bae Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of cellular biochemistry Vol.108 No.5
<P>Active calcium transport is carried out by calcium channel proteins, cytosolic buffering or transfer proteins, and pump proteins. Several components of this transport system have recently been verified using gene knockout (KO) models. We previously generated calbindin-D9k (CaBP-9k) KO mice and reported that induction of expression of some calcium transport proteins can compensate for the CaBP-9k gene deficiency. In the current study, we have further clarified the compensatory regulation of calcium transport genes by two calcium regulating hormones, 1,25-dihydroxyvitamin D<SUB>3</SUB> (1,25(OH)<SUB>2</SUB>D<SUB>3</SUB>) and parathyroid hormone (PTH), in CaBP-9k KO mice, because the levels of these hormones differ between the KO and wild-type (WT) mice. The induction of transient receptor potential cation channel, subfamily V, member 6 (TRPV6) in the duodenum was observed in adult KO male mice but induction was not modified by physiologic doses of 1,25(OH)<SUB>2</SUB>D<SUB>3</SUB>. Duodenal TRPV6 transcription in WT and female KO mice were modulated by 1,25(OH)<SUB>2</SUB>D<SUB>3</SUB> in a dose-dependent manner. This compensatory gene induction was not detected in the mice fed a vitamin D<SUB>3</SUB>-deficient diet. Compensatory gene induction was not affected by PTH. Thus, the compensatory expression of duodenal TRPV6 in the KO male mice may be tightly correlated with serum 1,25(OH)<SUB>2</SUB>D<SUB>3</SUB>. Vitamin D receptor (VDR) transcription and protein levels were measured to examine whether VDR expression mediates differential regulation of duodenal TRPV6 between WT and KO mice, but expression and levels of VDR were similar in both genotypes. The compensatory TRPV6 transcripts in KO mice may be modulated by endogenous vitamin D<SUB>3</SUB> via other factors of VDR signaling complexes. J. Cell. Biochem. 108: 1175–1183, 2009. © 2009 Wiley-Liss, Inc.</P>
과도안정도와 미소신호안정도 관점에서 국내 전력계통의 임계관성계수 평가
백종오(Jong-Oh Baek),정인주(In-Joo Jeong),하정민(Jung-Min Ha),맹창엽(Chang-Yeop Maeng),권오근(Oh-Geun Kwon),이배근(Bae-Geun Lee),김수배(Soobae Kim) 대한전기학회 2019 전기학회논문지 Vol.68 No.9
The increase of inverter-based renewable energy sources reduces inertia constant of power systems and the loss of the system inertia may cause power system stability problems. To ensure a secure energy delivery system, these problems should be analyzed thoroughly from various perspectives about power system stability. This paper presents the estimation of the critical inertia of Korean power systems, which is the minimum amount of inertia required to maintain power system dynamic securities. The estimation has been made by gradually reducing the system inertia of Korean power systems and then by analyzing the change of dynamic securities in terms of transient and small signal stabilities. In the transient stability aspect, the decrease of critical clearing time(CCT) has been analyzed with four different reduced inertial models of Korean power systems. With the small signal stability perspective, the change of the system damping ratio has been studied. Based on the results from the stability studies, the critical inertia has been determined and it can be considered as the indirect limit of renewable energy shares in Korean power systems.
RTP1, a Rat Homologue of Adenovirus ElA-associated Protein BS69, Interacts with DNA Topoisomerase II
Oh, Misook,Rha, Geun-Bae,Yoon, Jeong-Ho,Sunwoo, Yang-Il,Hong, Seung-Hwan,Park, Sang-Dai The Korean Society for Integrative Biology 2002 Korean journal of biological sciences Vol.6 No.3
Topoisomearse II is an essential enzyme in all organisms with several independent roles in DNA metabolism. Recently, it has been demonstrated that the C-terminal region of topoisomerases II is associated with hetero-logous protein-protein interactions in human and yeast. In this study, we identified that RTP1, a rat homologue of EIA binding protein BS69, is another topoisomerae II interacting protein by yeast two-hybrid screening. RTP1 has an E1A-binding domain and a MYND motif, which are known to be required for transcriptional regulation by binding to other proteins and interaction with the leucine zipper motif of topoisomerase II. The physical interaction between RTP1 and topoisomerase ll$\alpha$ was examined by GST pull-down assay in vitro. The expression level of RTP1 peaks in S phase as that of topoisomerase ll$\alpha$. These results suggest that the interaction between topoisomerase ll$\alpha$ and RTP1 might play an important role in regulating the transcription of genes involved in DNA metabolism in higher eukaryotes.