선박 초기설계 단계에서의 설계 및 해석 프로세스 통합

한중수(Joong Soo Han),최영(Young Choi) 대한조선학회 2005 大韓造船學會 論文集 Vol.42 No.3

RNN 알고리즘을 이용한 보행분석 기반 개인 식별방법 구현

한중수(Joong Soo Han),임을규(Eul Gyu Im) 한국정보과학회 2017 한국정보과학회 학술발표논문집 Vol.2017 No.06

기호 실행 기반 응용프로그램 추론 기법을 이용한 프로토콜 역공학 방법 제안

한중수(Joong Soo Han),임을규(Eul Gyu Im) 한국정보과학회 2018 한국정보과학회 학술발표논문집 Vol.2018 No.6

HPLC 에 의한 암의 지표로서의 뇨중 Ribonucleoside 의 분리 및 정량에 대한 연구

박경남(Kyung Nam Park),한중수(Joong Soo Han),고재경(Jai Kyung Koh) 대한내과학회 1987 대한내과학회지 Vol.33 No.2

N/A Urine contains certain metabolic end products of nucleic acid metabolism including sma1l amounts of modified nucleosides. They are methylated or otherwise structurally transformed ribosyl purines and pyrimidines and are not recycled through the salvage pathway but are excreted in urine. Their measurement in urine provides an accurate indicatar of the metabolism of RNA, especially transfer RNA. Altered patterns of excretion of these compounds might offer a sensitive biomarker for diagnosis and treatment of cancer. In order to find out whether the urinary ribonucleosides could be used as tumor markers for the hepatocellular carcinoma or stomach cancer, analysis of urine samples from normal controls and patients with hepatocellular carcinoma and stomach cancer was performed using reversed-phase HPLC. For this study, a rapid and precise chromatographic method for the determination of ribonucleosides in urine by high-performance liquid chromatography has been developed, The ribonucleosides were first isolated with an affinity gel containing immobilized phenylboronic acid. Contents of pseudouridine, 5-methylcytidine, 1-methyladenosine, 7-methylguanosine, guanosine, 1-methylinosine and 1-methylguanosine were significantly increased in urine of patients with hepatocellular carcinoma. Among these elevated ribonucleosides, excretions of pseudouridine, 5-methylcytidine, 1-methylinosine and 1-methylguanosine were higher than the upper limits (mean+2SD) in control group providing positive rates above 78% (positive rates for the excretions of 5-methylcytidine and 1-methylinosine were 100 %). In patients with stomach cancer, urinary excretions of pseudauridine, cytidine, 5-methylcytidine, 7-methylguanosine and adenosine were significantly in- creased. Positive rates for those of pseudouridine and 5-methylcytidine were above 50o. The results suggest that urinary levels of pseudouridine, 5-methylcytidine, 1-methylinosine and 1-methylguaaosine for the hepatocellular carcinoma and those of pseudouridine and 5-methylcytidine for the stomach cancer are useful as diagnostic tumor markers.

소아 아토피피부염에서 Phospholipase D1의 SNP 발굴 및 연관성

신수아 ( Su A Shin ),박신영 ( Shin Young Park ),조주환 ( Joo Whan Cho ),한중수 ( Joong Soo Han ),오재원 ( Jae Won Oh ),이하백 ( Ha Baik Lee ) 대한소아알레르기 및 호흡기학회 2007 소아알레르기 및 호흡기학회지 Vol.17 No.3

분만시 모체 및 제대혈청의 유산 탈수소효소 활성과 유산탈수소효소 동위효소의 변동에 관한 연구

한중수 全北大學校 學徒護國團 1981 全國大學生學術硏究發表論文集 Vol.6 No.-

68 healthy pregnant women delivered in Han Yang University hospital were subjected for this study. The present study was carried out to investigate the effect of delivery on the maternal and cord serum LDH, its isoenzymes, GOT and GPT activities. The LDH activity in maternal and cord serums in labor had been determined by method of Cabaud-Wroblewski and LDH isoenzyme was separated by electrophoresis. Followings are the results: 1) The mean maternal serum lactic dehydrogenase activity in labor was 245.0±75.4 units, which was significantly higher than mean serum LDH activity of nornmal non-pregnant women (221.0±73.4 units) (P<0.05) 2) The mean LDH activity in cord serum was 467.8±203.2 units, which was significantly higher than that obtained from maternal serum in labor (P<0.05) During the study two of three cases, in which maternal serum LDH activity was rather higher than that obatined from cord serum were nuchal cord. 3) In comparison with normal non-pregnant women, lactic dehydrogenase isoenzymes 4 and 5 in percent were elevated in labor, and lactic dehydrogenase 1, 2 and 3 in percent were relatively decreased. 4) Lactic dehydrogenase isoenzymes 3, 4 and 5 in percent were higher than thoes of maternal serum in labor. Relatively LDH isoenzyme 1 in percent was significantly lower than that. 5) Serum GOT and GPT activity of maternal serum in labor were 22 ± 6.3 units., 11.9 ± 4.1 units, respectively, which were within normal range. 6) GOT and GPT activity in cord serum were 35.3 ± 16.7 units, 11.9 ± 15.6 units respectively, in which GOT activity was much higher than that of maternal serum in labor.

난소암조직 Acid Deoxyribonuclease의 분리와 성상에 관한 연구

김두상,김영신,고재경,한중수 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

In order to find out a possible role of acid deoxyribonuclease (DNase) in carcinogenesis of the ovary, activity of the enzyme was measured and the nature of the enzyme was studied in serous cystadenocarcinoma and endodermal sinus tumor of the ovary following the purification of the acid DNase in the tumor tissue of the ovary. (1)The acid DNase activity was greatly increased in the tumor tissues of the ovary, serous cystadenocarcinoma and endodermal sinus tumor tissues, while neutral and alkaline DNase activities were unchanged in the tumor tissues. This may indicate that the acid DNase can be used as a biochemical marker for the ovary tumors. (2)Proteins in the tumor tissues of the ovary were separated by a DEAE-cellulose column chromatography into 7 peak, respectively, of which one peak protein each appeared to be specific for serous cystadenocarcinoma and endodermal sinus tumor. (3)Acid DNases in the tumor tissues of the ovary were isolated into a single peak, respectively. The size of peak in the tumor tissues was greater than that in the control tissue of the ovary, indicating that the acid DNases in the tumor tissues were activated, but not specific for the tumors. (4)Acid DNases in the tumor tissues of the ovary were partially purified by centrifugation and a DEAE-cellulose column chromatography. The purified enzyme was highly active against double stranded DNA (ds-DNA), even though some activity was found against single stranded DNA (ss DNA). Observations that acid DNase from serous cystadenocarcinoma tissue was highly active and specific to ds DNA compared with ss DNA suggested that the enzyme might play a role in transforming normal ovarian cells into cancer cells.

흰쥐 간장에 존재하는 카드뮴 결합 단백질의 정제 및 아미노산 조성에 관한 연구

한중수 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.2

In order to investigate whether the newly synthesized cadimium(Cd)-bound metallothionein(MT) accumulates in livers of rats which have received subcutaneous injections of Cd, isolation and purification of these protein were performed using Sephacryl S-200 and DEAE-Sephadex column chromatographies, and SDS-PAGE. And PICO.TAG HPLC analysis of amino acids was carried out to find out amino acid composition of MT-II. To confirm that the newly synthesized components are protein in nature, the Cd-bound fractions were examined for incorportation of [??S]cysteine. The effect of actinomycin-D on the synthesis of MT was also studied. 1. Cd induced newly synthesized peak which was identified by gel filtration on a Sephacryl S-200 column. This peak was detectabel only by measurement of Cd and zinc by atomic absorption analysis, and was labelled with [??S]cysteine. These results indicated that the peak components were protein which bound with Cd and zinc, e.g. MT. 2. MT was resolved into two isoforms i.e. MT-I and MT-II that had an apparent molecule mass of 10,000 by ion-exchange chromatography on a DEAE-Sephadex A-25 column 3. Formation of MT was completely abolished by actinomycin-D treatment. 4. SDS-polyacrylamide gel electrophoresis of MT-II showed the presence of a single band which appeared as a single peak on scanning at 590nm. 5. The most typical characteristic of MT-II was a extremely high cysteine content(32.8%) followed by lysine, serine and alanine. Also MT-II showed completely lacking in aromatic amino acids and histidine. These results suggested that newly synthesized MT-II containing high cysteine content was theh major isometallothionein and might have protective role against the toxic effects of Cd through the formation of metal-thiolate complexes and that the induction of formation of MT was due to the de novo synthesis of the protein after the induction ofmRNA synthesis by Cd.

급성 백혈병환아의 혈청내 변형 Ribonucleoside의 변동에 관한 연구

한중수,이정국,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

In order to find out whether the modified ribonucleosides in serum could be used as specific tumor markers for acute lymphocytic leukemia (ALL) and acute myelogenous leukemia (AML), analysis of serum samples from normal child hood controls and patients with the leukemia was performed using reversedphase HPLC. For this study, a rapid and precise chromatographic method for the determination of very small amounts of modified nucleosides in serum by HPLC has been developed. The ribonucleosides were prefractionated with boronate affinity gel column. 1. The concentration of total modified nucleosides in serum of patients with ALL was increased by 46% as compared to normal control level. Patients with ALL had significantly higher concentrations of pseudouridine (ø), 1-me-thyladenosine(m A), 4-thiouridine(sU), 1-methylinosine(mI), 1-methylguanosine(m G ), N,N-dimethylguanosine(m G ) and -methyl-adenosine( ) than normals. Among these nucleosides elevated, positive rates of ø, m A, m I, m G and N A as a marker for the leukemia were observed to be 79%,79%,64%,93% and 93%, respectively. 2. When compared to normal control value, total modified nucleoside content in serum of patients with AML was increased by 21%, Contents of , mA, sU, mI,mG and NA were significantly incresed in serum of patients with AML. Positive rate of mI, mG and NA were 83%, and that of mA was 67%, respectively in AML studied in this experiment. 3. In follow-up study for seven of fourteen patients with ALL, contents of , mA, mI and mG were decreased to normal value after effective chemotherapy. The results suggested that serum levels of ø, m A,m I, m G and N A for ALL and those of m A, m I, m G and N A for AML could be used as diagnostic or biochemical markers. These markers might be useful to monitor the effectiveness of chemotherapy for leukemia.

토끼 간조직의 Stimulatory Guanine Nucleotide 결합 단백의 αsubunit 에 대한 cDNA 염기서열에 관한 연구

이상훈,노연희,한중수,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

G proteins, a family of guanine nucleotide-binding protein superfamily, are involved in transmembrane signaling systems as transducers. In this study cDNAs encoding αsubunit of the stimulatory G protein (G α ) from rabbit liver were cloned and their nucleotide sequences were determined : a λgt 11 cDNA library from total cellular poly (A)' mRNA in rabbit liber was screened for recombinant λDNA that codes for G α with labeled probe. The probe used was an EcoRI digested 3' end fragment of cDNA for G α from olfactory neuroepithelium of rat. Six of the approximately recombinant clones were screened by in situ hybridization and by autoradiography, detecting sixteen positive clones. These clones were tested using polymerase chain reaction (PCR) technique and thirteen of them were turned out to have two sets of conserved sequence in αcDNA. Four clonse among them were selected form analysis of their cDNA sequences, showing the presence of two types of cDNA, namely and with the total length of nucleotide sequence of1.392bp and 1.613bp, respectively . From nucleotide sequence analysis the amino acid residues of -1 and α-2 were deduced : they contain 335 and 386 amino acid residues (including the initiator methionine), respectively. The calculated molecular weights for and were 38.9kd and 44.6kd, respectively. The significant difference in size between two cDNAs for G αseemed to be due to the assumption that alternative promoter and leading exon might be involved in transcribing the mRNA for . Two types of cDNA from rabbit liver shared over 95% of amino acid homology with that from rat olfactory neuroepichelium if deleted portions are not counted. More information on the cDNAs could be obtained through further studies such as Sl nuclease protection experiment, expression and mutation study.