Identification of Genes Connected with the Sensitivity to 5-FU and Cisplatin in Squamous Cell Carcinoma Cell Lines

최나영,김옥준,이금숙,김병국,김재형,장윤영,임원봉,정민아,최홍란,Choi, Na-Young,Kim, Ok-Joon,Lee, Geum-Sug,Kim, Byung-Gook,Kim, Jae-Hyeong,Jang, Youn-Young,Lim, Won-Bong,Chong, Min-A,Choi, Hong-Ran Korean Academy of Orofacial Pain and Oral Medicine 2005 Journal of Oral Medicine and Pain Vol.30 No.3

Squamous cell carcinoma (SCC) in head and neck show a variability in the response to chemotherapy, even when it present with similar histological tumor type, grade, and clinical stage. The purpose of present study it to identify predictive bio-marker for the sensitivity or resistance to conventional chemotherapeutic agents, 5-fluorouracil (5-FU) and Cisplatin Oral cancer cell lines were used in present study. MTT assay was performed to evaluate the sensitivity and/or resistance to 5-FU and Cisplatin. And RT-PCR was carried out for evaluation of the mRNA expressions of various genes associated with mutation, inflammation (COX pathway), cell cycle, senescence and extracellular matrix (ECM). The molecules which are correlated with the sensitivity to 5-FU are XPA, XPC, OGG, APEX, COX-2, PPAR, Cyclin E, Cyclin B1, CDC2, hTERT, hTR, TIMP-3, TIMP-4 and HSP47. And the molecules are correlated with the sensitivity to Cisplatin are COX-1, iNOS, eNOS, PCNA, collagen 1 and MMP-9. Taken together, when choosing the appropriate chemotherpeutic agents for patients, considering the molecules which are correlated or reversely correlated is helpful to choose the resonable agents for cancer patients.

Evaluation of the body weight and laying performance of diallel crossed Korean native chicken layers from hatch to 40 weeks of age

최나영 충남대학교 농업과학연구소 2022 Korean Journal of Agricultural Science Vol.49 No.4

This study investigated quality characteristics of chicken stock with added plum extract (PE) and green tea powder (GP) stock during storage. Plum extract (0, 0.5, 1, 3%) and green tea powder (0, 0.5, 1, 3%) were added at three levels. Chicken stock was stored at room temperature for 14 days. The pH of the chicken stock decreased significantly as the content of PE and GP increased (p < 0.05). The group with 3% plum extract added showed significantly lower pH values (p < 0.05). Total numbers of microorganisms (TMC) showed significant differences according to the storage period (p < 0.05), where the groups with PE and GP added showed lower TMC values than the control group, This indicates that PE and GP could inhibit the growth of microorganisms. The addition of 3% PE or GP decreased lipid oxidation (TBARS) and protein deterioration (VBN) values. In sensory test, the results showed that adding PE or GP has a positive effect on storage characteristics of chicken stock. The addition of PE rather than GP is effective not only in improving sensory evaluation, but also in minimizing changes in quality by suppressing lipid oxidation and protein spoilage during storage. In conclusion, 3% PE addition was found to be the most optimal supplementation choice for increasing the storability of chicken stock.

Cell Surface Properties and Biofilm Formation of Pathogenic Bacteria

최나영,배영민,이선영 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Cell surface properties of slime production, cell hydrophobicity (CH), colony spreading, auto-aggregation, and biofilm formation (BF) of pathogens were investigated. Staphylococcus aureus, Pseudomonas aeruginosa, and Listeria monocytogenes generally formed strong biofilms and were positive slime producers. The extracellular polymeric substances isolated from agar-grown biofilms of Bacillus cereus and P. aeruginosa contained a high proportion of carbohydrates, proteins, and extracellular DNA. High levels of CH were observed for all pathogens when hydrocarbons were combined with ammonium sulfate (AS). Levels of hydrophobicity based on bacterial adhesion to hydrocarbons using nonane with AS were positively correlated with hydrophobic interaction chromatography assay and contact-angle measurement results. BF and CH values of all pathogens, except E. coli O157:H7 and B. cereus, were highly correlated. Levels of pathogen hydrophobicity differed depending on the measurement method. Factors affecting BF were different depending upon the pathogen.

The Role of Double Inversion Recovery Imaging in Acute Ischemic Stroke

최나영,박순찬,이정민,류창우,장건호 대한자기공명의과학회 2019 Investigative Magnetic Resonance Imaging Vol.23 No.3

Purpose: The purpose of this study was to investigate if double inversion recovery (DIR) imaging can have a role in the evaluation of brain ischemia, compared with diffusion-weighted imaging (DWI) and fluid-attenuated inversion recovery (FLAIR) imaging. Materials and Methods: Sixty-seven patients within 48 hours of onset, underwent MRI scans with FLAIR, DWI with b-value of 0 (B0) and 1000 s/mm2, and DIR sequences. Patients were categorized into four groups: within three hours, three to six hours, six to 24 hours, and 24 to 48 hours after onset. Lesion-to-normal ratio (LNR) value was calculated and compared among all sequences within each group, by the Friedman test and conducted among all groups, for each sequence by the Kruskal- Wallis test. In qualitative assessment, signal intensity changes of DIR, B0, and FLAIR based on similarity with DWI and image quality of each sequence, were graded on a 3-point scale, respectively. Scores for detectability of lesions were compared by the McNemar’s test. Results: LNR values from DWI were higher than DIR, but not statistically significant in all groups (P > 0.05). LNR values of DIR were significantly higher than FLAIR within 24 hours of onset (P < 0.05). LNR values were significantly different between, before, and after six hours onset time for DIR (P = 0.016), B0 (P = 0.008), and FLAIR (P = 0.018) but not for DWI (P = 0.051). Qualitative analysis demonstrated that detectability of DIR was higher, compared to that of FLAIR within 4.5 hours and six hours of onset (P < 0.05). Also, the DWI quality score was lower than that of DIR, particularly relative to infratentorial lesions. Conclusion: DIR provides higher detectability of hyperacute brain ischemia than B0 and FLAIR, and does not suffer from susceptibility artifact, unlike DWI. So, DIR can be used to replace evaluation of the FLAIR-DWI mismatch.

영상매체에 재현된 고려여인의 수발양식(修髮樣式)에 관한 연구

최나영,최은미,양정순,이윤진 한국피부과학연구원 2009 대한피부미용학회지 Vol.7 No.4

The purpose of this study was to examine Koryo women’s hairstyle reproduced in visual media and to explain the importance of historical investigation and the reproduction of hairstyle, which should be changed according to age. For this study, we analyzed three historical dramas with the early, mid and late periods of the Koryo Dynasty as their background, investigated hairstyle in the Koryo Dynasty using old literature, Buddhist paintings from the Koryo Dynasty, figure paintings on walls, and relics, and collected data related to visual media using photographs on the Internet. According to the results of this study, the hairstyle in drama ‘Queen Cheonchu’ with the early Koryo Dynasty as its background should have reproduced Gogye, the hairstyle in the Shilla Dynasty because, during its early period, Koryo established all its systems based on those from Shilla. However, the hairstyle in Queen Cheonchu seems to have reproduced the hairstyle in the late Koryo Dynasty rather than that in its early period. Court ladies in drama ‘The Age of Warriors’ with the mid Koryo Dynasty as its background show hairstyle quite similar to the style of the women in Munhigwihwando. According to Koryodogyeong (1123) written during the reign of the 17th King Injong, which is the same period as the Age of Warriors, gisaengs’ hairstyle was called Chumagye. The hairstyle of gisaengs appearing in The Age of Warriors also reproduced Chumagye well. In movie ‘Ssanghwajeom’ with the late Koryo Dynasty as its background reproduced Princess Noguk, who was King Gongmin’s wife, and her hairstyle was similar to that of Princess Noguk in her portrait. However, while the hairstyle in the queen’s lady attendants in Gwangyeongseobunbyeonsangdo painted in the late Koryo Dynasty was tied up, the hairstyle of court ladies in the movie is similar to that of the noble woman in Gwangyeongbonbunbyeonsangdo. According to relics from the late Koryo Dynasty, court ladies’ hairstyle is believed to be closer to Gogye. 이 연구는 영상매체에 재현되어진 고려여인의 수발양식을 살펴보기 위해 고려시대의 전기, 중기 그리고 후기를 배경으로 한 세편의 사극을 선정하여 분석하였다. 고려시대의 수발양식은 고문헌, 고려불화, 벽화인물도 그리고 유물 등을 중심으로 살펴보았고 영상매체에 관련된 자료는 인터넷 검색을 통해 사진 자료를 수집하여 비교하였다. 연구결과 고려 초를 배경으로 한 천추태후의 수발양식은 오히려 고려 초의 고계보다는 고려말의 수발양식을 재현한듯하다. 고려 중기를 배경으로 한 무인시대의 상궁의 수발양식은 문희귀환도의 여인의 머리장식과 매우 흡사하며 기녀의 수발양식도 고려도경(1123)에 묘사되어진 기녀의 머리형태인 추마계로 잘 재현해 놓았다. 고려 말을 배경으로 한 쌍화점은 노국공주의 모습을 유사하게 재현하였다. 그러나 왕실시녀의 수발양식은 고려 말에 그려진 관경서분변상도에 의하면 고계형태에 더 가까울 것으로 생각되어진다.

한국 인삼제품에 대한 외국인의 인식도와 구매의도의 관계 분석

최나영,한상준,장광진,Choi, N.Y.,Han, S.J.,Chang, K.J. 국립한국농수산대학교 교육개발센터 2018 현장농업연구지 = Journal of practical agricultural resear Vol.20 No.1

This study was initiated because many studies would be necessary to promote Korean ginseng products when the sales of Korean ginseng are declining and many Korean ginseng farmers are facing economic difficulties. The increase in domestic sales is important, but this study is targeting for foreigner's perceptions of Korean ginseng and how to improve the products. The goal of this study is to analyze how to increase their purchase intentions of Korean ginseng products. Questionnaire survey research methods were used to learn more about making certain improvements to ginseng products, consumers are hoping, how affect their perceptions and purchase intentions of Korean ginseng, as well as how foreigners' perceptions of Korean ginseng products affects purchase intentions. Because it's difficult to find any studies about foreigner's Korean ginseng purchase intentions, the survey was expanded to include how people take care of their health, the accessibility of purchasing ginseng products, psychological factors as well as consumer characteristics, satisfaction with Korean ginseng, and intentions of buying Korean ginseng products. The result of the analysis on how making certain desired improvements to and perceptions of Korean ginseng products affect purchase intentions indicated that Korean ginseng perceptions highly affected the purchase intentions; however making certain improvements to ginseng products showed a low impact on the purchase intentions. In other words, making certain improvements, such as convenience of the ginseng products, doesn't affected Korean ginseng purchase intentions directly. On the other hand, it showed that the usefulness, its high quality, and safety trust of Korean ginseng have a big impact on purchasing intentions. If the ginseng that is grown and processed in Korea is better promoted for its high value, the Korean ginseng farmers who are facing many economic difficulties will be able to raise their income.

제3차 영재교육진흥종합계획에 따른 예술영재교육의 성과평가

최나영,이경진,김성혜 한국영재학회 2019 영재교육연구 Vol.29 No.2

The purpose of this study is to evaluate the performance of gifted education in the arts from 2013 to 2017, the period of the 3rd Master Plan for Promotion of Gifted Education. This evaluation has been conducted on objectives, 5 driving strategies, and 17 detailed tasks of the 3rd Master Plan. Additionally, this study aims to suggest policy proposals and practical strategies in order to improve the national gifted education in the arts. The study investigates research reports and papers on gifted education in the arts, annual plans of gifted education centers, and statistical data of the Gifted Education Database run by Korean Educational Development Institute. It analyzes results of the expert opinion survey which consisted of 33 questions. Achievements in gifted education in the arts during the period are as follows: expanding opportunities for the underprivileged, diversification of identification instruments, and developing programs for creativity, integration, or career education. However, outcomes from thorough researches have failed to settle in the educational field, evaluation and consulting have been superficially conducted, and the authority has provided teachers with little reward. Based on the result, the study provides some suggestions of policy’s communicability, qualitative monitoring, flexible implementation, and accountability based on the establishment of the proper reward system. 본 연구의 목적은 2013년부터 2017년까지 실시된 제3차 영재교육진흥종합계획의 목표와 5개 분야 17개 추진과제에 근거하여 예술분야의 성과와 한계를 평가함으로써 현재 실행 중인 제4차 영재교육진흥종합계획 하에서의 예술영재교육의 정책적·교육적 실천의 개선 방안을 제안하는 것이다. 이를 위하여 영재교육종합데이터베이스의 통계자료, 예술영재교육 연구자료, 예술영재교육기관의 문서자료 등을 분석하였다. 또한 과제별로 총 33개 문항을 구성하여 전문가와 현장교원을 대상으로 의견조사를 실시하였다. 그 결과 소외계층 대상 예술영재교육 기회 및 지원 확대, 교사·학부모 관찰·추천도구와 자기평가도구 타당화 등 선발 도구의 다양화, 예술영재교육 프로그램 구성·운영 다양화를 위한 창의·융합형, 인성·진로교육 프로그램 개발의 양적 확대 등이 주요 성과로 나타났다. 반면, 선발방식의 다양화를 위한 제도 부족과 융합교육의 현장정착 미흡, 평가·컨설팅의 형식적 운영, 예술영재교육 담당 교원에 대한 보상체계 결여 또는 부족 등이 한계로 드러났다. 연구결과를 토대로 예술분야에서 국가 영재교육 정책의 현장 전달성 및 이해도·실행능력 제고, 정책의 현장 실행에 대한 질적 모니터링 강화, 예술분야의 특성을 고려한 영재교육 정책의 유연한 실행, 담당교원의 업무 부담을 가중시키는 구조의 개선을 제언하였다.

The Effects of a Nursing Simulation Education about Child Care with Acute Asthma Attacks on the Problem Solving Process, Self-efficacy and Learning Satisfaction for Nursing Students

최나영 사단법인 미래융합기술연구학회 2020 아시아태평양융합연구교류논문지 Vol.6 No.12

This study aimed to investigate the effects of nursing simulation education about child care with acute asthma attacks for nursing students. Nursing students that participated in this study were divided into experimental and control groups. The subjects in the control group received the video education and the subjects in the experimental group received the simulation education to observe the differences in the problem solving processes, self-efficacy and learning satisfaction. This study used a non-equivalent control pre-post design and data were analyzed using SPSS WIN 22.0. The general characteristics and the values of the variables along with percentages and standard deviation were analyzed. In addition, the subjects homogeneity and t-test hypotheses were analyzed. The difference in the subjects’ scores before and after the video education program were statistically significant in the self-efficacy(t = 3.08, p < .004) and learning satisfaction(t = 3.46, p <.003). The findings indicate that this simulation would be useful in improving self-efficacy and learning satisfaction for child care with asthma attack in nursing students.

백워드 디자인을 통해 본 미국 국가 수준 핵심음악기준의 내용 제시 방식의 의미

최나영 한국음악교육공학회 2015 음악교육공학 Vol.- No.25

This study aims to analyze the way to present contents of the National Core Music Standards in the U.S. based on the backward design by Wiggins and McTighe, and to provide some suggestions for the national music curriculum in Korea. From the literature review, the role of curriculum to support teachers' instruction, forms of presenting curriculum contents, and core ideas of the backward design were covered. The analysis result shows that the National Core Music Standards in the U.S. adopts the way of presenting both the integrated contents of arts curriculum and the music curriculum contents. Additionally it provides enduring understandings and essential questions as curriculum contents, which is quite distinctive compared to the previous national curriculum. This change enhances clarity of the curriculum compared to the presentational form providing performance standards alone. Thus the national music curriculum should play a role to support teachers and their instructions by both more clearly and hierarchically presenting curriculum contents and providing relationship with other subjects. As for the future study, the research about systems to support and evaluate the quality of the curriculum contents and the curricular coherence between the national level and the class level is needed. Also a study of the curriculum alignment between contents and assessment to promote enduring understanding in music is needed. 본 연구는 최근 개정된 미국의 국가 수준 핵심음악기준의 교육내용 제시 방식을 Wiggins와 McTighe의 교육과정 개발 이론인 백워드 디자인의 관점에서 살펴보고 우리나라 국가 교육과정의 개발에 시사점을 제공하는 것을 목적으로 하였다. 선행연구 고찰을 통해 교사의 수업을 지원하는 국가 교육과정의 역할과 이를 위한 교육내용 제시 방식의 유형, 교육과정상에서 교육내용과 관련된 백워드 디자인의 주요 아이디어를 살펴보았다. 분석 결과 음악교과와 타 예술교과를 통합하여 교육내용을 제시하는 방식과 음악교과 별도의 교육내용을 제시하는 혼합 방식을 사용하였다. 또한 영속적 이해와 핵심질문을 교육과정의 교육내용으로써 함께 제시한 점이 과거와 달라진 주요 변화이며, 내용기준으로서 영속적 이해의 제공은 수행기준만 제시되었을 때보다 교육내용을 명료하게 하고 있었다. 따라서 교육내용을 보다 명료하게 위계적으로 제시하고, 포괄적으로 제시된 교육내용을 교실 수준으로 적절히 해석·적용할 수 있도록 함으로써, 그리고 교과 통합적 측면을 보여줌으로써 교사를 지원하는 국가 교육과정으로서의 역할을 충실히 해내야 할 것이다. 한편 국가 수준에서 제시하는 교육내용 자체의 질적 수준 담보는 물론 교과용 도서 개발 시 단원 수준에 적절한 교육내용으로 연결될 수 있도록 하는 지원·평가체제에 대한 후속연구, 교육과정상의 평가가 교육내용과 일관성을 갖고 수업활동에서 구체화될 수 있도록 하는 후속연구가 필요하다.

간호대학생을 위한 영아 응급간호 시뮬레이션 프로그램의 개발 및 효과

최나영 사단법인 한국융합기술연구학회 2024 아시아태평양융합연구교류논문지 Vol.10 No.3

본 연구는 간호대학생의 영아 응급간호 시뮬레이션 프로그램을 개발하고 응급간호 지식, 임상 판단 및 임상 수행능력의 효과를 확인하고자 시행되었다. 대상자는 실험군과 대조군으로 나뉘었으며 대조군은 강의식 수업만을 받고 실험군은 강의식 수업과 시뮬레이션 수업을 받아 응급간호 지식, 임상 판단과 임상 수행능력의 차이를 분석하였다. 본 연구는 비 동등성 대조군 사전-사후 설계이며 자료는 SPSS WIN 26.0을 사용하여 분석하였다. 일반적 특성과 변수들은 퍼센트와 표준편차로, 대상자의 동질성과 가설은 χ²과 paired t-test로 분석하였다. 본 연구는 학생 당 10시간(강의 2시간, 시뮬레이션 8시간) 시행되었다. 영아 응급간호 시뮬레이션은 3개의 수업(프리브리핑, 러닝, 디브리핑)으로 구성되었다. 연구결과는 임상 판단과 임상 수행능력이 유의미하게 개선되었다. 그러므로, 타당성이 검증되고 표준화된 영아 응급간호 시뮬레이션 프로그램은 대학생의 시뮬레이션 훈련을 위해 사용될 수 있다. This study aimed to develop an emergency care simulation program for nursing college students based on their knowledge of emergency care, clinical judgment, and clinical practice ability. Nursing students who participated in this study were divided into experimental and control groups. The subjects in the control group received only lecture education, and the experimental group received lecture and simulation education to observe the differences in their knowledge of emergency care, clinical judgment, and clinical practice ability. This study used a non-equivalent control pre-post design, and the data were analyzed using SPSS WIN 26.0. The general characteristics, values, percentages, and standard deviations were analyzed. In addition, the subject’s homogeneity and hypotheses were analyzed with χ² and paired t-tests. This research program was applied for 10 hours (lecture education: 2 hours and simulation program: 8 hours) per person. The emergency care simulation program for infants was provided in three classes (pre-briefing, running, and debriefing). The results indicated that clinical judgment and clinical practice ability for nursing simulation significantly improved. The emergency care simulation program for infants for nursing college students positively affected student’s clinical judgment and clinical practice ability. Therefore, this validated and standardized emergency care simulation program for infants can be used to train simulations of college students.