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유채 식물체 내로의 카드뮴 이행과 산화스트레스 경감에 미치는 아스코베이트 효과구명
정하일 ( Ha-il Jung ),채미진 ( Mi-jin Chae ),이은진 ( Eun-jin Lee ),이태구 ( Tae-gu Lee ),김명숙 ( Myung-sook Kim ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-
The role of ascorbate (AsA) in antioxidant defense system-associated resistance to cadmium (Cd) in oilseed rape plants is not clearly demonstrated until recent study. The present study investigated the critical role of exogenous AsA on the biochemical responses of reactive oxygen species (ROS) and antioxidant levels in oilseed rape (Brassica napus L. cv. Tammi) seedlings exposed to Cd. Cadmium (10 uM) alone treatment led to significant reductions in plant growth; increase the level of superoxide, hydrogen peroxide, and malondialdehyde; and increase the uptake and accumulation of Cd by the roots and shoots in hydroponically grown 10-day-old seedlings. Moreover, it reduced AsA content and AsA redox ratios, which have been correlated with the reduction in glutathione (GSH) and/or nicotinamide adenine dinucleotide phosphate (NADPH) redox status. However, the exogenous AsA application to Cd-exposed seedlings decreased Cd-induced ROS, improved antioxidant defense systems by increasing AsA, GSH, and NADPH contents, and increased Cd uptake and accumulation in both roots to shoots of the plants. These results proved that the enhancement in AsA redox status can be linked to an increase in the GSH and/or NADPH redox ratios through induction of the AsA-GSH-NADPH cycle. Thus, these results suggest that exogenous AsA application to oilseed rape seedlings under Cd stress alleviates the overall Cd toxicity by regulating the homeostasis of the AsA-GSH-NADPH cycle, which re-establish the cellular redox status.
Protox 저해형 제초제 저항성 형질환벼와 비형질전환벼의 미질 및 이화학적 성분 차이
정하일(Ha-Il Jung),윤영범(Young Beom Yun),권오도(Oh-Do Kwon),이도진(Do Jin Lee),백경환(Kyoungwhan Back),국용인(Yong In Kuk) 韓國雜草學會 2012 Weed&Turfgrass Science Vol.32 No.1
본 연구의 목적은 Protox 저해형 제초제 저항성인 형질전환벼계통(MX, PX, AP37)과 비형질전환벼(WT) 백미에 대한 미질, 무기물, 구성아미노산, 유리아미노산 및 유리당 함량을 조사하였다. 형질전환벼계통의 완전미, 식미값, 단백질 및 백도(MX와 AP37제외)는 비형질전환벼에 비해 높거나 유사하였고, 형질전환벼 계통의 분상질립, 균열립 및 쇄미(PX와 AP37제외)는 비형질전환벼에 비해 낮았다. 그러나 피해립과 아밀로스함량에서는 형질전환벼계통과 비형질전환벼간에 유의적인 차이가 없었다. PX의 K 함량과 PX와 AP37의 Cu 함량은 WT에 비해 적었을 뿐 일부 다른 무기물에서는 WT보다 형질전환벼계통에서 오히려 높거나 유의적인 차이가 없었다. 대부분 구성 아미노산 함량은 형질전환벼계통에서 WT에 비해 높았거나 유사하였고, 단지 AP37의 Isoleucine 함량만이 WT에 비해 적었다. 한편 유리아미노산 Leucine과 Tyrosine의 경우는 PX와 AP37에서 그리고 총 유리아미노산의 함량의 경우는 PX에서만 WT에 비해 유의적으로 감소하였다. 그러나 형질전환벼 계통의 다른 종류의 유리 아미노산 함량은 WT과 유사하였다. MX와 PX의 Sucrose 함량은 WT에 비해 적었으나 형질전환벼계통의 fructose, glucose 및 maltose 함량은 WT에 비해 높거나 유사하였다. 따라서 Protox 유전자는 벼의 양분구성에 부정적인 영향을 미치지 않는다고 할 수 있다. Characteristics related to grain quality and physiochemical components such as mineral, total amino acid, free amino acid, and free sugar composition were investigated in Protox inhibitor resistanttransgenic rice (MX, PX, and AP37) and its nontransgenic counterpart (WT). Head rice, palatability, protein, and whiteness (except for MX and AP37) of milled transgenic rice were high or similar to those of the non-transgenic counterpart. Immature rice, unfilled grain, and cracked kernels (PX and AP37) of milled transgenic rice were lower than those of its non-transgenic counterpart. However, there were no significant differences in damaged grain between the transgenic rice lines and its counterpart. Potassium content in PX and copper contents in PX and AP37 were only low compared with their non-transgenic counterparts, but other mineral contents in transgenic rice lines were high or showed no significant differences compared with non-transgenic counterparts. Contents of most total amino acid composition in transgenic rice lines were high or similar to those in non-transgenic counterparts, but the content of isoleucine in AP37 was only low compared with its non-transgenic counterpart. On the other hand, free amino acid, leucine and tyrosine in PX and AP37, and total free amino acid in PX were low compared with their non-transgenic counterparts. However, the content of free amino acid in other kinds in transgenic rice lines were similar to those in their non-transgenic counterparts. Contents of sucrose in MX and PX were low compared with non-transgenic counterpars, but contents of fructose, glucose, and maltose in transgenic rice lines were high or similar compared with their non-transgenic counterparts. This results indicated that Protox genes had no negative affect on the nutritional composition of rice.
Anthracycline 계 항암성 항생물질 DA-125 의 Beagle dog 에 대한 26 주 반복정맥투여독성시험
정태천(Tae Cheon Jeong),한상섭(Sang Seop Han),양중익(Jung Ick Yang),차신우(Shin Woo Cha),하창수(Chang Su Ha),노정구(Jung Koo Roh),박종일(Jong Il Park),신호철(Ho Chul Shin),김형진(Hyoung Chin Kim) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.2
This study was performed to investigate the toxicity of DA-125 in beagle dogs, an anthracycline antitumor antibiotic. The dogs were administered DA-125 i.v. at 0.0023, 0.0375, 0.15 and 0.6 ㎎/㎏/day, 6 days/week for 26 weeks. At 0.6 ㎎/㎏, all male and female dogs were either sacrificed moribundly or dead during the 26-week treatment. The dogs revealed inactivity, salivation, dark bloody discharge, swelling of the subcutaneous injection site, abscess, and ulceration in the abdominal wall and legs. At 0.15 ㎎/㎏, anorexia, salivation, and swelling of the injection site were observed. The food consumption was decreased with a statistical significance at 6 and 12 weeks treatment in males of 0.6 ㎎/㎏. At 0.0375, 0.15 and 0.6 ㎎/㎏, body weights were decreased significantly in a dose-related fashion after 17 weeks treatment. Total white blood cell counts for male dogs at 0.6 ㎎/㎏ were lower than those of control dogs after 13 weeks treatment, which appeared mainly due to decreased neutrophils. At 0.15 ㎎/㎏, testicular atrophy was found in all males by gross pathology and the testicular weights were significantly decreased when compared to those of control males. Microscopically, the testis showed moderate atrophy of the seminiferous tubules and marked decrease in number of spermatozoa in the epididymal tubules. At 0.6 ㎎/㎏, petechia or echymotic hemorrhage was observed in gastrointestinal tract, heart, lungs, and other organs at the necropsy. Marked atrophy of thymus were observed in both males and females. In addition, severe testicular atrophy was noted in all males. Microscopically, gastrointestinal tract showed hemorrhage, epithelial denudation, hypermucus secretion, and atrophy of intestinal villi. Seminiferous tubules of the atrophic testis were lined with Sertoli cells only and devoid of germ cells. Severe oligospermia or aspermia was present in the epididymal tubules. Bone marrow showed marked depletion of hemopoietic cells. In addition, marked atrophy was found in the lymphoid tissue of gastrointestinal tract, various lymph nodes, and thymus. Injection sites showed marked inflammatory response with necrosis, necrotizing vasculitis, thrombus formation, and ulceration in the skin. According to the present results, no observed effect level appeared to be 0.0375 ㎎/㎏. At 0.15 ㎎/㎏, testis was a target organ, while at 0.6 ㎎/㎏ hemopoietic tissue, gastrointestinal tract, and testis were considered to be target organs. At 0.6 ㎎/㎏ the test compound seems to inflict a damage on the blood vessels causing hemorrhage in the various organs and tissues.
생물전환된 품종별 대두 발효물의 주요 지표성분 함량 및 분석법 검증과 항산화 활성 평가
정태동(Tae-Dong Jung),신기해(Gi-Hae Shin),김재민(Jae-Min Kim),오지원(Ji-Won Oh),최선일(Sun-Il Choi),이진하(Jin-Ha Lee),이상종(Sang Jong Lee),허인영(In Young Heo),박선주(Seon Ju Park),김현태(Hyun Tae Kim),강범규(Beom Kyu Kang),이옥환(Ok- 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.5
본 연구에서는 생물전환에 의한 품종별 대두 5종의 isoflavone 함량, 총페놀 함량, 항산화능 및 isoflavone의 분석법 검증을 시행하였다. HPLC를 이용한 분석법 검증 결과 isoflavone 6종의 표준용액과 우람(S-3) 비발효 및 발효 추출물의 retention time이 일치하는 것을 확인하였으며, spectrum 분석 결과에서도 같은 spectrum을 보여 특이성을 확인하였다. 직선성 확인 결과 isoflavone 6종의 검량선의 상관계수 값은 0.9998~1.0000으로 나타나 우수한 직선성을 보여주었다. 정밀성 측정 결과 일내 정밀도에서 0.22~0.88%, 일간 정밀도에서 0.34~1.00%의 높은 정밀도를 나타냈다. 회수율 측정 결과 daidzin은 100.55~110.21%, glycitin은 100.26~112.35%, genistin은 97.67~111.89%, daidzein은 100.95~110.43%, glycitein은 98.56~106.02%, genistein은 101.29~110.25%의 회수율을 나타내었다. Daidzin, glycitin, genistin의 검출한계는 각각 0.14, 0.16, 0.20 ㎍/mL로 측정되었으며, daidzein, glycitein, genistein의 검출한계는 각각 0.25, 0.29, 0.20 ㎍/mL로 나타났다. Daidzin, glycitin, genistin의 정량한계는 각각 0.44, 0.48, 0.60 ㎍/mL를 나타냈고 daidzein, glycitein, genistein은 각각 0.75, 0.89, 0.61 ㎍/mL로 나타났다. 생물전환에 의한 대두 발효 시 배당체인 daidzin, glycitin, genistein이 모두 비배당체인 daidzein, glycitein, genistein으로 전환됨을 확인할 수 있었다. 각각의 isoflavone을 합한 값인 total isoflavone 함량은 대두 우람(S-3) 발효물에서 2,074.6 ㎍/g으로 가장 높은 함량을 나타냈으며 비발효 대두 새단백(S-5)에서 가장 낮은 119.8 ㎍/g의 total isoflavone 함량을 나타내었다. 총페놀 함량은 진풍(S-4) 비발효물이 44.96 mg GAE/g으로 가장 높은 총페놀 함량을 보였다. 항산화능 측정 결과 FRAP는 대풍2(S-2) 발효물에서 0.22로 가장 높은 값을 나타냈으며 새단백(S-5) 비발효물에서 0.15로 가장 낮은 값을 나타내었다. ORAC 지수도 FRAP와 유사하게 새단백(S-5) 비발효물에서 195.24±3.08 μM TE/g으로 가장 낮은 값을 보였으며 대풍2(S-2) 발효물에서 753.79±6.95 μM TE/g으로 가장 높은 값을 나타내었다. The present study evaluated the validation method for isoflavone contents of fermented soybean extracts by bioconversion as well as their antioxidant activities. Our results show that the total isoflavone contents of non-fermented and fermented soybean extract ranged between 119.8 to 637.7 ㎍/g and between 567.3 to 2,074.6 ㎍/g, respectively. Moreover, fermented soybean extracts had higher contents of isoflavone aglycones, including daidzein, glycitein, and genistein than non-fermented soybean extracts as well as lower contents of isoflavone glucosides such as daidzin, glycitin, and genistin. FRAP and ORAC values ranged between 0.15 to 0.22 and between 195.24 to 753.79 μM Trolox equivalents/g in non-fermented and fermented soybean extracts, respectively. These results indicate that fermented soybean extracts had higher total isoflavone contents and antioxidant activities than non-fermented soybean extracts. Bioconversion process in this study may have the potential to produce isoflavone-enriched natural antioxidant agents with high added value from soybean matrices.