한약 치료를 시행한 트리메틸아민뇨증 환자 1례 증례보고

이정은,한성준,이엄지,이재형,남성욱,하나연,김진성,Lee, Jung-eun,Han, Seong-jun,Lee, Eom-jee,Lee, Jae-hyung,Nam, Seong-uk,Ha, Na-yeon,Kim, Jin-sung 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.2

Objective: This case reports on the efficacy of herbal medicine treatment for trimethylaminuria.Method: A 29-year-old female Korean patient with trimethylaminuria received herbal medicine treatment for three months. We evaluated her symptoms with the methacholine challenge test and OralChroma.Results: The odor intensity shown in the methacholine challenge test demonstrated improvement.Conclusion: Herbal medicine treatment could improve trimethylaminuria symptoms.

양성 고페닐알라닌혈증 환자에 대한 임상적 고찰과 유전자 분석

이정은,이정호,이동환,Lee, Jeongeun,Lee, Jeongho,Lee, Dong Hwan 대한유전성대사질환학회 2016 대한유전성대사질환학회지 Vol.16 No.2

목적: 본 연구의 목적은 국내 양성 고페닐알라닌혈증의 돌연변이 유전자 분석, 임상 양상, 치료에 대해 조사하는 것이다. 방법: 2010년도 4월부터 2015년도 8월까지, 신생아 선별검사에서 페닐알라닌의 상승으로 본원으로 전원되어 양성 고페닐알라닌혈증으로 진단된 10명의 환자를 대상으로 시행되었다. 조사항목으로는 환자들의 특징 및 임상 양상과 직접 염기서열 분석을 통한 PAH 유전자 돌연변이 형태 분석을 포함하였다. 결과: 10명의 환자들 중 2명은 고페닐알라닌혈증으로 진단된 자녀의 부모였으며, 환자들 중 특이 과거력이나 임상 증상이 있는 경우는 없었다. 혈장 아미노산 분석에서는 페닐알라닌이 1.2 mg/dL부터 4.2 mg/dL까지의 범위로 측정되었다. BH4 부하검사에서는 모든 환자들이 BH4에 무반응이었다. 확진 검사로 시행한 페닐알라닌수산화효소 유전자 분석에서는, 총 20개의 대립유전자들 중에서 11개의 각기 다른 대립유전자형을 확인할 수 있었는데, 이 중 제일 흔한 대립유전자는 R53H (c.158G>A)였다. 뿐만 아니라, 직접염기서열분석 상에서 2가지의 새로운 돌연변이 형태인 V423A (c.1268T>C)와 V51A (c.152T>C)를 발견하였다. 본 연구에 포함된 환자들은 약물을 복용하거나 식사 조절을 하지 않았음에도, 지속적인 외래 추적 관찰 결과 모든 환자들의 페닐알라닌 치는 6 mg/dL 이하로 유지되었으며, 정상적인 신경 인지발달을 하고 있는 것을 확인하였다. 결론: 이 연구는 양성 고페닐알라닌혈증 환자에 대한 분석이며 국내에서 처음으로 시행한 것이다. 모든 양성 고페닐알라닌혈증 환자들은 지속적인 치료 없이도 혈장 페닐알라닌 치를 6 mg/dL 이하로 유지하였고 정상적인 신경 인지발달을 하였다. 그러므로, 돌연변이 검사를 시행하여 양성 고페닐알라닌혈증 환자를 페닐케톤뇨증과 감별함으로써 불필요한 식사 요법이나 약물 투여를 줄여 환자의 삶의 질을 높일 수 있다. Purpose: This study aimed to analyze genetic mutations, clinical manifestations, and treatment of patients with benign HPA in Korea. Methods: This case series study involved ten HPA patients who were referred to our hospital because of high phenylalanine concentration. We investigated their demographic features, clinical manifestations, and mutations of the PAH gene through direct DNA sequencing. Results: Among ten patients with benign HPA, two pairs of patients were related (father-daughter, mother-daughter relationship) cases, and all of them showed no specific clinical manifestations or notable past history. Their plasma phenylalanine levels ranged between 1.2 and 4.2 mg/dL. In the tetrahydrobiopterin (BH4) loading test, all patients were nonresponsive to BH4. In the confirmation test of PAH mutation analysis, we identified eleven different alleles out of twelve. The most common allele was R53H (c.158G> A). In addition, two novel PAH gene mutations, V423A (c.1268T>C) and V51A (c.152T>C), were identified. Although the patients did not receive any pharmacologic treatment or continuous phenylalanine restriction dietary therapy, their neurocognitive development was normal. Moreover, on serial outpatient follow-up tests, all patients maintained phenylalanine levels below 6 mg/dL. Conclusion: This study is the first in Korea to analyze benign HPA patients. All patients with benign HPA could maintain phenylalanine levels below 6 mg/dL with normal neurocognitive development, without continuous therapy. Therefore, performing mutation analysis and distinguishing benign HPA from phenylketonuria (PKU) are important to help improve life quality in patients with benign HPA by avoiding unnecessary lifelong therapy.

Si 나노입자에서 shell이 전기화학적 특성에 미치는 영향

이정은,구정분,장보윤,김성수,Lee, Jeong-eun,Koo, Jeong-boon,Jang, Bo-yun,Kim, Sung-Soo 한국전기전자재료학회 2016 전기전자재료학회논문지 Vol.29 No.4

Effects of $SiO_x$ or C shells on electrochemical properties of Si nanoparticles were investigated. $SiO_x$ shells with thickness of 10~15 nm were formed on homogeneously crystalline Si nanoparticles. Incase of Si-C nanoparticles, there were 30~40 layers of C with a number of defects. Li-ion batteries were fabricated with the above-mentioned nanoparticles, and their electrochemical properties were measured. Pristine Si shows a high IRC (initial reversible capacity) of 2,517 mAh/g and ICE (initial columbic efficiency) of 87%, but low capacity retention of 22%, respectively. $SiO_x$ shells decreased IRC (1,534 mAh/g) and ICE (54%), while the retention increased up to 65%, which can be explained by irreversible phases such as $LiO_2$ and $Li_2SiO_3$. C shells exhibited no differences in IRC and ICE compared to the pristine Si but an enhanced retention of 54%, which might be from proper defect structures.

Disciplinary Health Education : 서울시 일부 고등학생들의 AIDS 에 대한 지식 , 태도 , 위험행동에 관한 조사 연구

이정은,홍양자 ( Jung Eun Lee,Yang Ja Hong ) 한국체육학회 1994 국제스포츠 학술대회 Vol.1994 No.-

직장인 스트레스 반응 척도 개발 및 타당화 연구

이정은,최보라,정영은,송광헌,강민재,채정호,Lee, Jeong Eun,Choi, Bora,Jung, Young-Eun,Song, Gary,Kang, Min Jae,Chae, Jeong-Ho 대한불안의학회 2012 대한불안의학회지 Vol.8 No.2

Objectives : The purpose of this study was to develop an employee's stress response scale (ESRS) and examine its validity and reliability. Methods : The study was conducted as follows : item generation, and test of validity and reliability. Items were developed via literature review, review of instruments, and data acquired from email-survey of counselors in the field of Employee Assistance Program (EAP). In order to test validity and reliability, data were collected from 400 employees. Results : The result of exploratory factor analysis of ESRS suggested 4 factor structures (work-related, anger, somatization, depression & anxiety) with a total of 25 items. ESRS showed a relatively strong positive correlation with Hospital Anxiety-Depression scale (HAD), Perceived Stress Scale (PSS), and Psychological Wellbeing Index (PWI), which showed ESRS had high convergent validity. Cronbach's ${\alpha}$ and test-retest reliability was .95 and .610 respectively. Conclusion : The ESRS is reliable and valid brief scale for measuring stress responses of employees. This scale would be useful in quantitatively evaluating job stress in workplace.

유령멍게(Ciona intestinalis)와 노랑꼭지유령멍게(Ciona savignyi) 에탄올 추출물의 항산화 효과

이정은,강상모,Lee, Jeong-Eun,Kang, Sang-Mo 중소기업융합학회 2021 융합정보논문지 Vol.11 No.4

유령멍게와 노랑꼭지유령멍게는 국내의 경우, 항만, 선박의 바닥, 양식장에 대량으로 발생하여 큰 피해를 입혀, 위해종으로 지정되어 있다. 따라서 이들을 이용하는 측면에서 화장품 원료로의 사용 가능성을 검토하였다. 유령멍게와 노랑꼭지유령멍게를 70% 에탄올로 추출물을 제조하여 항산화능을 측성하였다. 유령멍게와 노랑꼭지유령멍게 추출물 20 mg/mL에서 DPPH는 각각 71.80%, 21.40%를 보였고, ABTS는 각각 95.47%, 27.53%, nitric oxide 생성 억제능은 각각 97.47%, 88.90%로 나타났다. 총 페놀 함량은 유령멍게와 노랑꼭지유령멍게에서 각각 0.081, 0.041 mg gallic acid/mg extract으로 나타났다. 결과적으로 노랑꼭지유령멍게보다 유령멍게에서 다소 높은 항산화능을 보였다. 이들은 추출물은 동물성 유래 항산화물질들로 화장품 소재로서 활용 가능함을 확인하였다. Ciona intestinalis and Ciona savignyi have been designated as hazardous species because they have caused great damages as they appeared in ports, ships, and farms in Korea in large amount. Therefore, in terms of using them, the possibility of use as cosmetic ingredients was examined. C. intestinalis and C. savignyi were treated with 70% ethanol to make an extract and then their antioxidant activity was measured. C. intestinalis and C. savignyi showed 71.80% and 21.40% of DPPH at 20mg/mL of extract, respectively. ABTS was 95.47% and 27.53%, while nitric oxide production inhibitory ability was 97.47% and 88.90%, respectively. Total phenol content was 0.081 and 0.041 mg gallic acid/mg extract, respectively. As a result, C. intestinalis showed somewhat higher antioxidant activity than C. savignyi. It is confirmed that these extracts are animal-derived antioxidants that can be used as cosmetic materials.

생명 과학I 수업에서 과학 글쓰기 활동이 고등학생의 과학적 사고력에 미치는 영향

이정은,정은영,Lee, Jungeun,Jeong, Eunyoung 경북대학교 과학교육연구소 2013 科學敎育硏究誌 Vol.37 No.3

The purpose of this study was to investigate the effect of science writing activities on high school students' scientific thinking ability in Life Science I class. In order to do this, 6 teaching-learning materials dealing with science writing and an evaluation tool for scientific thinking ability were developed. And the subjects were 224 high school students of 6 classes. As a result of applying science writing activities in Life Science I class, the students' scientific thinking ability was improved. And students' inductive/deductive/critical/creative thinking ability was improved. In addition, in the most of the evaluation criteria of scientific thinking ability, the scores of posttest were higher than those of pretest. The number of students to show higher performance levels was increased. Therefore, science writing activities have positive effect on high school students' scientific thinking ability. This study provides some implications for teaching science writing activities to develop students' scientific thinking ability. 과학교육의 목표가 지식의 습득에서 지식 형성과정을 강조하는 것으로 변화하면서, 학생들에게 과학적 사고 연습의 기회를 제공하는 학습 방법으로 과학 글쓰기가 부각되고 있다. 현행 과학과 교육과정에서는 과학 글쓰기와 토론을 통하여 과학적 사고력, 창의적 사고력 및 의사소통 능력을 함양할 수 있도록 지도할 것을 명시하고 있다. 본 연구는 생명 과학I 수업에서 과학 글쓰기 활동이 학생들의 과학적 사고력에 어떤 영향을 미치는지 알아보고자 하였다. 이를 위해 생명 과학I 수업에 활용 가능한 6차시의 과학 글쓰기 활동지와 과학적 사고력 평가도구를 개발하였다. 고등학교 1학년 6학급 224명 학생을 대상으로 하여 3개의 학급에는 과학 글쓰기를 활용한 수업을 하고 다른 3개의 학급에서는 강의식 수업을 총 6차시 실시하였다. 생명 과학 I 수업에 과학 글쓰기 활동을 적용한 결과, 학생들의 과학적 사고력이 향상되었고, 과학적 사고력의 영역별로 분석한 결과, 귀납적 사고력, 연역적 사고력, 비판적 사고력, 창의적 사고력의 4가지 범주 모두에서 유의미한 향상을 보였다. 과학적 사고력 영역의 평가 준거별 분석 결과에서는 귀납적 사고력의 경우 평가 준거 '근거'와 '설명'에서 향상되었고, 연역적 사고력에서는 평가 준거 '결론'과 '설명'에서 유의미하게 향상되었다. 비판적 사고력에서는 평가 준거 '주장', '근거', '설명', '반증'에서, 창의적 사고력에서는 '창의성', '융통성', '독창성', '정교성'의 모든 평가 준거에서 유의미한 향상이 나타났다. 본 연구의 결과는 과학적 사고력 함양을 위한 과학 글쓰기 활동의 지도에 대한 시사점을 제공하며, 다양한 과학 글쓰기 활동을 위한 자료 개발에 도움이 될 것이다.

국내 20대 여성의 애슬레저 웨어(Athleisure wear) 착용실태 조사

이정은,최세린,도월희,Lee, Jeongeun,Choi, Selin,Do, Wolhee 한국의류산업학회 2017 한국의류산업학회지 Vol.19 No.5

This study investigates the wearing behavior and athleisure wear design preferences of women in their twenties as related to the design development of athleisure wear. A questionnaire survey was conducted on 185 women in their 20s. The survey was conducted from September to October 2016. Questionnaire items consisted of respondents' general items, athleisure wear and preference related items, and athleisure wear purchase related items. We collected 185 questionnaires from participants; subsequently, 169 were used as analytical data (except for incomplete questionnaires). The results of the study are as follows. Fit and wear comfort was the most important factor in the selection of athleisure wear. As a result of investigating the popularity of the athletic wear brand, the preference for the total sportswear brand was higher than the professional athletic wear brand. When the preferred colors were examined, the achromatic colors were ranked higher than chromatic colors. An examination of the preferred designs according to items indicated that the bra top preferred the 'U' shaped front plate and the 'Y' base basic type; in addition, T-shirts with the round neckline and the 1/4 length sleeves were preferred as tops. Leggings preferred long length type. It is necessary to develop a brand that matches the preference of the twenties' consumers by adjusting the aged target that can avoid the overheated competition and develop a niche market.

청금강화탕(淸金降火湯 ) 및 과루지실탕(瓜蔞枳實湯)이 호흡기(呼吸器) 배상세포(杯狀細胞)로부터의 뮤신 분비(分泌)에 미치는 영향

이정은,박양춘,Lee, Joung-Eun,Park, Yang-Chun 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.2

Objective : This study is intended to investigate whether the two oriental medical prescriptions, CheongGeumGangHwa-tang(CGGH) and GwaRuJiSil-tang(GRJS), significantly affect mucin release from cultured hamster tracheal surface epithelial(HTSE) cells. Materials and Methods : Confluent HTSE cells were metabolically radio labeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of CGGH or GRJS to assess the effect of each agent on 3H-mucin release. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase(LDH) release. Also, the effects of CGGH and GRJS on contractility of isolated tracheal smooth muscle were investigated. Results : (1) CGGH and GRJS significantly increased mucin release from cultured HTSE cells, without cytotoxicity : (2) CGGH and GRJS did not affect contractility of isolated tracheal smooth muscle. Conclusions : These results suggest that the effects of CGGH and GRJS should be further investigated, and that it would be gainful to invesigate, from among oriental medical prescriptions, what novel agents have these mild expectorant effects on mucin secretion from airway goblet cells.

생쥐 초기배아의 유전자 활성에 미치는 Protein Kinase Inhibitors의 영향

이정은,채영규,배인하,윤용달,김문규,Lee, Jeong-Eun,Chai, Young-Gyu,Bae, In-Ha,Yoon, Young-Dal,Kim, Moon-Kyoo 대한생식의학회 1995 Clinical and Experimental Reproductive Medicine Vol.22 No.2

Transcriptional activation of the embryonic genome initiates at 2-cell stage in mouse embryo and is characterized by the synthesis of TRC which is restricted to 2-cell stage. To investigate the roles of various protein kinases on the embryonic gene activation, the effects of protein kinase inhibitors on in vitro development and protein synthetic profiles of the early mouse embryos were examinded. None of ${\alpna}-amanitin$ which is a mRNA synthetic inhibitor, H8 which is a PKA inhibitor, and H7 which is a PKC inhibitor, affected on first cleavage of mouse 1-cell embryos in vitro. However, all of these drugs inhibited the second cleavage. When the drugs were removed following treatment for 6 hours, H8 or H7 treatment showed little inhibition on subsequent development of 1-cell embryos to 2-cell stage or further. In contrast, ${\alpna}-amanitin$ irreversibly inhibited the development of 1-cell embryos to 2-cell stage following removal of the drug. Genistein, a TPK inhibitor, inhibited both the first cleavage of 1-cell embryos and the second cleavage of 2-cell embryos, suggesting that TPK activity may be important during the early cleavages. All of the above four drugs inhibited TRC synthesis as shown by the fluorographic analysis of $[^{35}S]-Met$ labeled protein profiles. When late 1-cell embryos were treated with H7 and analyzed synthetic patterns of $[^{35}S]-Met$ labeled protein, the quantitative differences of protein synthesis on SDS-PAGE appeared on 77 kD and 33 kD region at $32{\sim}38$ hours post hCG. From these studies, transcriptional activation of embryonic genome is not essenting to the mouse 1-cell embryos to develop to 2-cell stage. Hawever, TPK activity is reguisite for both the first cleavage and second cleavage. Similarly, both PKC and PKA activities are required for the second cleavage of mouse embryos, but not for the first cleavage.