Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

이성호,홍승현,박승한,Sung-Ho Lee,김보람,김동희,박승우 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.75 No.6

We have developed a high-speed and high-resolution multimodal nonlinear optical microscope, which can acquire in vivo label and label-free 3D images. Using this microscope, we could successfully observe dynamic images of the zebrafish heartbeat with 33-millisecond intervals without staining process, indicating 1.68 beats per second. In addition, we show that the label-free, high-speed, real-time images can be obtained during somatic cell divisions in the zebrafish’s embryo.

KASINICS 광학계의 고스트 분석

이성호,육인수,진호,박수종,한정열,이대희,공경남,조승현,박영식,박장현,한원용,Lee, Sung-Ho,Yuk, In-Soo,Jin, Ho,Pak, Soo-Jong,Han, Jeong-Yeol,Lee, Dae-Hee,Kong, Kyung-Nam,Cho, Seung-Hyun,Park, Young-Sik,Park, Jang-Hyun,Han, Won-Yong 한국천문학회 2005 天文學論叢 Vol.20 No.1

The reimaging optics of the KASINICS (KASI Near Infrared Camera System) includes many transparent components like an entrance window, band-pass filters, and blocking filters. As observational targets or in-field background objects, bright stars may cause optical ghosts that can significantly degrade the system performance of the KASINICS. We estimated analytically the relative brightness of ghost components with respect to a point source and examined the effects of tilting optical components as a method of suppressing ghosts. We also performed numerical ray tracings including all the optical components and found the results are consistent with those of the analytic estimations. We conclude that the KASINICS will not suffer from significant ghost effects with appropriate anti-reflection coatings and fittings for the optical components.

길이방향으로 연속된 래티스를 가지는 철선 트러스데크의 구조 거동

이성호,박형철,오보환,조순보,Lee, Sung Ho,Park, Hyung Chul,Oh, Bo Hwan,Cho, Soon Bo 한국강구조학회 2009 韓國鋼構造學會 論文集 Vol.21 No.1

철선 트러스데크는 기존의 재래식 슬래브 거푸집이나 일반 데크플레이트 공법을 대체하여 철골조는 물론 철근콘크리트 구조물에도 사용이 점차 증가하고 있다. 현재 사용되고 있는 기존의 트러스 데크는 래티스 철선과 하부 철선의 비연속적인 절점간격에 의한 트러스의 비렌딜거동에 의해 완전한 트러스형상에 비해 처짐이 커지는 문제점이 있다. 본 연구는 이러한 문제점을 해결하기 위해 래티스 철선과 상하부 철선이 연속적으로 연결되어 비렌딜거동이 없는 순수 트러스형태를 구현함으로써 처짐성능이 우수한 새로운 형태의 데크를 고안하였다. 개발된 시스템의 구조적 거동을 평가하고자, 해석 및 성능평가 실험을 수행하였다. 처짐에 영향을 주는 요소를 중심으로 래티스 철선의 길이방향 연결형태, 간격을 해석과 실험의 주요 변수로 설정하였다. 해석과 실험은 실제 시공 시 타설되는 콘크리트 하중이 아연도 철판을 통해 철선에 작용하는 것을 고려하여 아연도 철판에 직접 등분포 하중을 가력하였다. 구조해석 및 실험을 통한 분석결과 철선 트러스데크의 길이방향 형태, 즉 래티스 철선의 길이방향 연결 형태가 구조적 거동에 영향을 주는 주요한 인자로서 작용했으며, 래티스 철선을 길이방향으로 연속적으로 연결함으로써 비렌딜거동에 의한 영향을 감소시킬 수 있었다. 또한 래티스 철선의 연결간격을 증가 시킬 경우에도, 상, 하부 철선에 큰 응력증가 없이 우수한 처짐 성능을 발현 할 수 있었다. A truss deck system that has replaced the slab form conventional method has become widely used in the construction of reinforced concrete structures as well as steel structures. The current commercial products, however, have some problems. The discontinuity between the lattice wires on the joint of the bottom wire induces vierendeel behavior, which increases the deflection of the system. In this research, a new truss deck system with continuous lattice wires on the level of the bottom wire was developed to reduce the system's vierendeel behavior and to improve its deformation capacity. To investigate the system's structural behavior, an experimental test and an analysis were performed. The main parameters of the test and analysis were the longitudinal shape and spacing of the lattices. To simulate the loading condition in the construction field, uniform construction loads were directly applied on the deck plates of the analysis model and the test specimens. The results of such analysis and test revealed that the longitudinal shape of the lattice wires is a major factor affecting the structural behavior of a steel wire truss deck. Thus, continuous lattice wires could result in decreased vierendeel behavior in the steel wire truss deck. It was also found that the truss deck system with lattices spaced longer than in the conventional products could be effectively used without increasing the member stresses.

ICP-MS에 의한 Tungsten Matrix 중의 미량원소분석

이성호,Lee, Sung-Ho 대한화학회 2008 대한화학회지 Vol.52 No.6

고속철도에 있어서의 정보시스템 구현 사례에 대한 연구

이성호,안중호,Lee, Sung-Ho,Ahn, Joong-Ho 한국경영정보학회 2005 Information systems review Vol.7 No.2

대한민국의 고속철도통합정보시스템(IRIS) 구축 사업은 2000년 12월 23일부터 2004년 12월 30일까지 4년여에 걸쳐 총 일천여 억 원의 예산과 총인원 10,000M/M 이상의 인력을 투입하고 18,000여 본의 프로그램을 개발한 초대형 프로젝트로서 크게 응용 시스템 개발, 인프라 아키텍처, 그리고 역무자동화 장비 구축 등으로 구분하여 추진되었다. 본 연구에서는 첫째, IRIS의 구축 배경과 전체적인 내용 및 시스템 이미지를 제시한 후 개별 시스템들에 대한 각각의 주요 내용을 제시하였다. 둘째, 성공적인 프로젝트 추진을 위한 프로젝트 관리방법론과 시스템 개발방법론을 적용한 특징 및 내용을 살펴보았다. 마지막으로 IRIS 사업의 주요 포인트와 향후 발전 방향을 논의하였다. 이 프로젝트의 전체적 시스템 구축 및 운영 성과에 대한 평가는 향후 시스템을 운영하면서 좀 더 자세히 분석 및 연구되어야 할 것이다. Integrated Railroad Information System(IRIS) Construction Project is one of the super projects in Korea required to invest over 100 billion won and 10,000 engaged manpower until Dec 30, 2004. Composition of this paper is as follows; firstly, examined individual contents and aspects of IRIS, and studied formality and contents that have been based to project methodology for successful propulsion of project in overall situation of SI project progress until present. Secondly, presented for construction background of IRIS as well as Project Management and Project Development Methodologies. Thirdly, described each characteristic and development substance for individual systems after present whole contents and system image of the system. Finally, presented successful propulsion result of this project and development directions. We presented development example laying stress on IRIS's Project contents and propulsion circumstances. Evaluation about operation results should be analyzed and studied in detail operating system from now on.

해외직접구매 이용자의 지각된 가치가 만족 및 재이용의도에 미치는 영향

이성호,한상린,Lee,Seong Ho,Han,Sang-Lin 한국소비문화학회 2015 소비문화연구 Vol.18 No.2

오늘날 인터넷 및 모바일쇼핑이 생활화되고 있으며, 더불어 글로벌한 경영환경과 함께 소비생활도 글로벌화되면서 해외직접구매 시장이 더욱 활기를 띄고 있다. 하지만 이처럼 해외직접구매가 활성화되고 있음에도 불구하고 학술적 연구는 거의 전무한 실정이다. 그러므로 본 연구는 새롭게 급부상하고 있는 해외직접구매를 중심으로 해외직접구매가 소비자에게 가져다주는 가치들을 조사하고 해외직접구매에 대한 만족 및 이용의도에 미치는 영향력 을 살펴보고자 한다. 즉, 해외직접구매에 대한 소비자의 지각된 가치로 경제적 가치, 감성적 가치, 편의적 가치, 사회적 가치가 해외직접구매에 대한 쇼핑만족에 미치는 영향과 이러한 만족이 해외직접구매에 대한 지속적인 이 용의도에 어떠한 영향을 미치는가를 분석하여 해외직접구매에 대한 초기수용자들의 요구 및 기대와 해외직접구매 활성화에 대한 마케팅 측면의 중요성을 제시하고자 한다. 실증분석을 위해 해외직접구매를 실제로 이용하는 이용자들을 대상으로 설문을 통해 데이터를 수집하였으며, 소비자의 지각된 가치 → 해외직접구매에 대한 쇼핑만족 → 해외직접구매에 대한 재이용의도에 대한 인과적 구성 모델에 대해 구조방정식 모델분석으로 검증하였다. 분석결과 소비자의 지각된 가치 중 편의적 가치를 제외한 나머지 모두 해외직접구매 쇼핑만족에 긍정적인 영향을 미쳤으며, 그 중 경제적 가치가 해외직접구매 쇼핑만족에 가장 큰 영향을 주는 것으로 분석 결과 나타났다. 또한 해외직접구매에 대한 쇼핑만족은 해외직접구매를 지속적으로 이용하려는 재이용의도를 증가시키는 것으로 나타났다. 이러한 연구결과는 본 연구에서 논의된 바와 같이 학문적으로 소비자의 지각된 가치가 해외직접구매를 이용하는 소비자들에게 매력적인 요인이 되는지를 파악하여 그들은 어떠한 요구 및 기대를 가지고 있는지를 파악 하였다. 또한 실무적으로는 소비자가 느끼는 가치를 관리할 수 있는 구체적인 방안을 제시하여 관련업계에 대해 중요한 마케팅적 시사점을 제공한다. As customers usually use internet and mobile shopping easily today and consumption trend is changing to globalize, overseas direct purchase shopping is growing in Korea. Although people are interested in the overseas direct purchase shopping, there are no research dealing with it. Therefor, this study focus on perceived value of customer and research the impact on Consumers’ shopping satisfaction and Repurchase intention. That is, we can get needs and expectation of customers as using overseas direct purchase shopping, and we give some marketing insights in order to vitalize the market of overseas direct purchase shopping. This study attempted to investigate the impact of consumers’ perceived values on overseas direct purchase shopping. The purpose of this study is to examine how four perceived value constructs of consumers related to overseas direct purchase shopping(economic value, emotional value, convenience value, social value) affect consumers’ shopping satisfaction and repurchase intention using overseas direct purchase shopping. For this study, data were gathered from respondents who use overseas direct purchase shopping and analyzed by structural equation model. All three constructs except for convenience value about perceived value(economic value, emotional value, social value) affected consumers’ shopping satisfaction and repurchase intention toward overseas direct purchase shopping. Among the three constructs, the economic value is most important factor to affect consumers’ shopping satisfaction and repurchase intention. The results show that management and investment to improve perceived value(economic value, emotional value, social value) of overseas direct purchase shopping make consumers’ shopping satisfaction toward overseas direct purchase shopping, and then the satisfaction can improve repurchase intention of overseas direct purchase shopping. These findings have important theoretical implication how perceived value is attractive factor on using overseas direct purchase shopping and practical implications which are suggest to manage and develop customers’ perceived value as a important marketing strategy in this paper.

흰쥐의 시상하부외 지역에서의 Growth Hormone Releasing Hormone (GHRH) 유전자발현;뇌하수체내 국부인자로서 Lactotroph분화에 관여할 가능성에 대하여

이성호,Lee, Sung-Ho 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.3

Biosynthesis and secretion of anterior pituitary hormones are under the control of specific hypothalamic stimulatory and inhibitory factors. Among them, Growth Hormone Releasing Hormone (GHRH) is the major stimulator of pituitary somatotrophs activating GH gene expression and secretion. Human GHRH is a polypeptide of 44 amino acids initially isolated from pancreatic tumors, and the gene for the hypothalamic form of GHRH is organized into 5 exons spanning over 10 kilobases (kb) on genomic DNA and encodes a messenger RNA of 700-750 nucleotides. Several neuropeptides classically associated with the hypothalamus have been found in the extrahypothalamic regions, suggesting the existence of novel sources, targets and functions. GHRH-like immunoreactivity has been found in several peripheral sites, including placenta, testis, and ovary, indicating that GHRH may also have regulatory roles in peripheral reproductive organs. Furthermore, higher molecular weight forms of the GHRH transcripts were identified from these organs (1.75 kb in testis; 1.75 and >3 kb in ovary). These tissue-specific expression of GHRH gene suggest the existence of unique regulatory mechanism of GHRH expression and function in these organs. In fact, placenta-specific and testis-specific promoters for GHRH transcripts which are located in about 10 kb upstream region of hypothalamic promoter were reported. The use of unique promoters in extrahypothalamic sites could be refered in a different control of GHRH gene and different functions of the translated products in these tissues. Somatotrophs and lactotrophs have been thought to be derived from a common bipotential progenitor, the somatolactotrophs, which give origins to either phenotypes. Although the precise mechanism responsible for the lactotroph differentiation in the anterior pituitary gland has not been yet clalified, there are several candidators for the generation of lactotrophs. In human, the presence of GHRH peptides with different size from authentic hypothalamic form in the normal anterior pituitary and several types of adenoma were demonstrated. Recently our group found the existence of immunoreactive GHRH and its transcript from the normal rat anterior pituitary (gonadotroph> somatotroph> lactotroph), and the GHRH treatment evoked the increased proliferation rate of anterior pituitary cells in vitro. The transgenic mouse models clearly shown that GHRH or NGF overexpression by anterior pituitary cells induced development of pituitary hyperplasia and adenomas particularly GH-oma and prolactinoma. Taken together, we hypothesize that the pituitary GHRH could serve not only as a modulator of hormone secretion but as a paracrine or autocrine regulator of anterior pituitary cell proliferation and differentiation. Interestingly enough, the expression of Pit-1 homeobox gene (the POU class transcription factor) was confined to somatotrophs, lactotrophs and somatolactotrophs in which GHRH receptors are expressed commonly. Concerning the mechanism of somatolactotroph and lactotroph differentiation in the anterior pituitary, we have focused following two possibilities; (1) changes in the relative levels or interactions of both hypothalamic and intrapituitary factors such as dopamine, VIP, somatostatin, NGF and GHRH; (2) alterations of GHRH-GHRH receptor signaling and Pit-1 activity may be the cause of lactotroph differentiation or pituitary hyperplasia and adenoma formation. Extensive further studies will be necessary to solve these complicated questions.

감육배관의 건전성평가 및 정비 관련 기술기준 고찰

이성호,이요섭,김홍덕,이경수,황경모,Lee, Sung Ho,Lee, Yo Seob,Kim, Hong Deok,Lee, Kyoung Soo,Hwang, Kyeong Mo 한국압력기기공학회 2015 한국압력기기공학회 논문집 Vol.11 No.2

Local wall thinning and integrity degradation caused by several mechanisms, such as flow accelerated corrosion, cavitation, flashing and/or liquid droplet impingement, is a main concern in secondary steam cycle piping system of nuclear power plants in terms of safety and operability. Thinned pipe management program (TPMP) has being developed and optimized to reduce the possibility of unplanned shutdown and/or power reduction due to pipe failure caused by wall thinning. In this paper, newest technologies, standards and regulations related to the integrity assessment, repair and replacement of thinned pipe component are reviewed. And technical improvement items in TPMP to secure the reliability and effectiveness are also presented.

증기발생기 급수링 관통손상 원인 및 영향 고찰

이성호,이요섭,Lee, Sung Ho,Lee, Yo Seob 한국압력기기공학회 2015 한국압력기기공학회 논문집 Vol.11 No.1

The function of Feed Water Ring is to provide the flow path from Feedwater Nozzle to inside of SG(steam generator). Significant amounts of general FAC on the outside of the Feed Water Ring are not likely due to the low flow velocities in this area. However, on the interior of the Feed Water Ring, there may be areas of local higher flow velocity which could lead to higher FAC rates. These may include the inlet tee from the Feedwater Nozzle into the Feed Water Ring, the areas where the Feed Water Ring changes diameter, and especially the entrance area to the J-Nozzles. In this paper, the results of root cause analysis of through-wall hole observed at domestic WH 51F SG Feed Water Ring and its effect on the integrity and performance of SG are described. And, the maintenance strategy for WH 51F SG Feed Water Ring and the monitoring strategy for Downcomer Feed Water Ring of CE System 80 SG are presented.

스키리조트의 명성이 다차원적 신뢰, 고객만족 및 구전확산에 미치는 영향

이성호,Lee, Sung-Ho 한국디지털정책학회 2014 디지털융복합연구 Vol.12 No.10

The purpose of this study is to ascertain the effect of the reputation of ski resorts on multidimensional trust, customer satisfaction and spread by word-of-mouth. To attain this goal, 400 adult male and female subjects using 3 Ski Resorts located in Gangwon-do in Korea in 2013 were selected. For this, convenient sampling was used. The data was processed with the 382 final available samples excluding the 18 inappropriate questionnaires through frequency-, exploratory factor-, reliability-, correlation-, and simple/multiple regression-analyses by using the PASW 18.0. The result is as follows. First, the reputation of the ski resorts was found to have a positive effect regarding the trust of the resorts and their employees. Second, trust in ski resorts and their employees was found to have a positive effect on customer satisfaction. Third, trust in ski resorts and their employees was found to have a positive effect on the spread of word-of-mouth about the resorts. Fourth, customer satisfaction was found to have a positive effect on the diffusion by word-of-mouth. 이 연구의 목적은 스키리조트의 명성이 다차원적 신뢰, 고객만족 및 구전확산에 미치는 영향을 규명하는 것이다. 이러한 목적을 달성하기 위해 2013년 강원도 지역에 소재한 3개(P, W, Y)의 스키리조트를 이용하고 있는 성인 남 여 400명을 조사대상으로 선정하였다. 이 때 사용한 표집방법은 편의표본추출법이다. 수집된 자료 중 부적절한 18부의 설문을 제외한 382개의 최종 유효표본을 가지고 빈도분석과 탐색적요인분석, 신뢰도분석, 상관관계분석 및 단순 및 다중회귀분석을 하기 위하여 PASW 18.0을 활용하여 자료처리를 하였다. 그 결과를 보면 다음과 같다. 첫째, 스키리조트의 명성은 리조트에 대한 신뢰와 리조트 종사원에 대한 신뢰에 긍정적인 영향을 미치는 것으로 나타났다. 둘째, 리조트에 대한 신뢰와 리조트 종사원에 대한 신뢰는 고객만족에 긍정적인 영향을 미치는 것으로 나타났다. 셋째, 리조트에 대한 신뢰와 리조트 종사원에 대한 신뢰는 구전확산에 긍정적인 영향을 미치는 것으로 나타났다. 넷째, 고객만족은 구전확산에 긍정적인 영향을 미치는 것으로 나타났다.