소 체외성숙 난자의 세포질내 정자주입에 의한 수정율 향상에 관한 연구

유상식,김용섭,이봉구,김상근 한국동물생명공학회(구 한국동물번식학회) 1998 Reproductive & developmental biology Vol.22 No.3

유기동물 관리 정책개발을 위한 발생원인 실증분석

유상식,배관표 서울행정학회 2022 한국사회와 행정연구 Vol.33 No.1

본 연구는 유기동물의 발생원인 탐색을 통해 보다 합리적인 유기동물 관리 정책을 개발하는 데에 기여하고자 수행되었다. 유기동물의 발생에 영향을 미치는 원인에 대하여 공공자료를 이용한 실증적 분석을 실시함으로써 다음과 같은 결과와 정책적 시사점을 얻었다. 첫째, 유기견과 유기묘의 발생에 영향을 미치는 요인은 서로 달랐다. 그러므로 유기동물 관련 정책을 다룰 때는 축종에 따라 서로 다른 관점에서 접근해야 할 것이다. 둘째, 가구소득의 감소와 유기견의 증가는 밀접한 관계가 있었다. 따라서 반려동물 보유세와 같이 가계에 경제적 부담을 부과하는 정책은 유기견의 발생을 증가시킬 우려가 있으므로 면밀한 검토가 필요할 것이다. 셋째, 거주지 이전과 시간적 여유의 증가는 유기묘의 감소에 유의한 영향을 나타냈다. 이는 1인 가구와 비경제활동인구가 유기묘 발생의 예방과 대응에 있어서 중요한 정책적 주체가 될 수 있음을 시사한다. 넷째, 본 연구는 가구 구성의 변화 중 이혼이 유기견과 유기묘의 발생에 모두 정의 영향을 나타낸다는 것을 확인하였다. 이러한 분석 결과는 지방자치단체 동물보호소에서 검토하고 있는 ‘반려동물 인수제’와 관련하여 의미가 크다. 본 연구는 유기동물이 발생하는 원인을 설문조사 자료가 아닌 경성 자료를 활용하여 밝힌 첫 연구로서 유기동물의 발생에 대한 이론적 논의의 발전에 기여하고 정책적 시사점을 제시한다는 점에서 의미가 있다.

난소적출 rat에 생약물질을 투여했을 때 호르몬 및 생화학치의 변동에 미치는 영향

유상식,김상근 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca- and P- deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600 ㎎/㎏, p.o), red ginseng(200 ㎎/㎏, p.o), safflower(200 ㎎/㎏, p.o), ipriflavone(80 ㎎/㎏, p.o) and estrogene(400 ㎍/㎏, i.m) for 5 weeks. During the treatment, the rats were examined for serum concentrations of estradiol, calcitonin, Ca, P, alkaline phosphatase(ALP) activities, body weight, femur ash and Ca contents. The results are summarized as follows: 1. The levels of serum 17 β-estradiol after five weeks of treatment were showed 39.6±3.0 pg/㎖ by antler-, 33.2±2.5 pg/㎖ by red ginseng-, 34.9±2.4 pg/㎖ by safflower-, 28.1±3.1 pg/㎖ by ipriflavone- and 40.6±3.0 pg/㎖ by estrogen-treated group. They, however, were signigicantly higher than 26.8±1.8 pg/㎖ of ovariectomized non-treatment group(p<0.05). 2. The levels of serum calcitonin after five weeks of treatment were showed 0.06±0.02 ng/㎖ by antler-, 0.55±0.04 ng/㎖ by red ginseng-, 0.59±0.02 ng/㎖ by safflower-, 0.56±0.04 ng/㎖ by ipriflavone- and 0.62±0.02 ng/㎖ by estrogen-treated group. However, they were significantly higher than 0.45±0.05 ng/㎖ of ovariectomized non-treatment group(p<0.05). 3. The levels of serum Ca contents after five weeks of treatment with antler, red ginseng, safflower, ipriflavone and estrogen were a little higher than 21.43±2.22 ㎍/㎖ of normal control group. 4. The serum P contents after five weeks of treatment were showed 12.11±2.14 ㎍/㎖ by antler-, 13.18±1.64 ㎍/㎖ by red ginseng-, 12.67±2.31 ㎍/㎖ by safflower-, 12.38±2.07 ㎍/㎖ by ipriflavone-, 11.86±1.93 ㎍/㎖ by estrogen-treated group. 5. The levels of serum ALP after five weeks of treatment were showed 164.8±3.8 IU/㎖ by antler-, 277.7±4.8 IU/㎖ by red ginseng-, 288.5±4.5 IU/㎖ by safflower-, 214.7±5.7 IU/㎖ by ipriflavone- and 159.4±5.4 IU/㎖ by estrogen-treated group. They were significantly higher than 144.1±3.5 IU/㎖ of normal control group(p<0.05). 6. The body weights of the antler-, red ginseng-, safflower-, ipriflavone- and estrogen-treated groups after five weeks of treatment, were 303.0±3.0g, 273.3±4.4g, 288.3±5.1g, 300.5±5.2g, 320.5±4.2g, respectively. 7. The weights of ashed left femora after five weeks of treatment were showed 0.36±0.01g by antler-, 0.40±0.01g by red ginseng-, 0.41±0.01g by safflower-, 0.36±0.01g by ipriflavone- and 0.35±0.01g by estrogen-treated group. Those results were significantly lower than 0.43±0.01g of normal control group. 8. The femoral Ca contents of the antler-, red ginseng-, safflower-, ipriflavone- and estrogen-treated rats were 19.6±0.7%, 17.8±1.1%, 18.8±0.9%, 18.0±1.1%, 18.3±1.1%, respectively, after five weeks of treatment. The femoral Ca contents of antler- and safflower-treated groups were higher than that of control group which showed 18.2±0.9% of Ca. Antler and safflower elevated serum estradiol and calcitonin, and decreased serum ALP significantly. Therefore they were thought to have therapeutic effect on osteoposis by making inhibitory effect on osteoclasts than activating osteoblasts.

한국 진도견의 serum protein 및 lipoprotein 변동에 관한 연구

유상식,이명헌,김상근 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The concentration of serum proteins and lipoproteins in Korean Jindo dogs were examined from the time of after birth to 150 days of age. Eight(4 females and 4 males) Jindo dogs were used in this study. Concentration of serum proteins and lipoproteins were fractioned by cellulose acetate electrophoresis. The results are summarized as follows; 1. The total serum protein concentrations at 1 days(4.6±0.4 g/dl) after birth were lower than in adult dogs, thereafter increased rapidly at 3 days(7.1±0.6 g/dl) of ages, and there was no significant changes between sexes. 2. The concentrations of serum protein from the time of after birth to 150 days of age were 0.35±0.1-8.5±0.2 g/dl and 0.5±0.1-0.8±0.2 g/dl, respectively. The γ-globulin concentrations were not detected 1 days after birth and increased rapidly after pups ingested colostrum(after 3 days of age), thereafter there was no significant changes of γ-globulin concentration after 10 days of age. 3. The concentrations of serum α, β-lipoprtein were not detected 1 days after birth and increased rapidly after pups ingested colostrum(at 3 days of age), from the time of after birth to 150 days of age were 49.1±11.6-94.1±16.4 ㎎/dl and 11.7±6.5-102.0±10.8 ㎎/dl, respectively. 4. the γ-lipoprtein concentrations no detected 1 days after birth and increased rapidly after pups ingested colostrum(at 3 days of age, 11.6±2.5 ㎎/dl) and decreased gradually during 50 days of age, and thereafter increased rapidly during 100-150 days of age(71.8±4.8-77.2±4.1 ㎎/dl).

身體의 自由 保障을 위한 被檢問者의 權利에 관한 考察

유상식 한국해사법학회 1999 해사법연구 Vol.11 No.2

行政作用과 適法節次에 관한 考察

유상식 한국해사법학회 1998 해사법연구 Vol.10 No.2

혈액의 구성방정식이 혈관유동특성에 미치는 영향

서상호,유상식 한국항공대학교 1995 論文集 Vol.33 No.-

혈관내 혈액유동현상을 파악하기 위해서는 생체를 이용한 실험적 연구가 필수적이지만 실험의 어려움으로 인하여 수치모사방법이 많이 이용되고 있다. 수치해석을 위해서는 지배방정식에 적용할 구성방정식이 필요한데 채택되는 구성방정식의 특성에 따라 수치모사결과가 다르게 나타난다. 본 연구에서는 가장 많이 이용되는 Carreau모델, 수정 Powell-Eyring모델, 수정 Cross모델 및 소정 멱법칙 모델을 채택하여 정상상태 하에서 원형관내유동을 수치모사하였다. 유동이 발달되는 입구길이 구간내에서는 채택된 구성방정식에 따라 중심선속도가 큰차리를 나타낸다. Carreau모델과 수정 Cross모델은 축방향을 따라 중심선속도와 압력강하가 네이놀즈수에 관계 없이 거의 동일하게 나타나지만, 수정 멱법칙모델은 레이놀즈수의 변화에 따라 중심선속도와 압력강하가 다르게 나타난다. Numerical simulation techniques are used in practice due to complication of the experimental study of blood flow in vivo. A constitutive equation should be applied to the governing equations of flows for numerical simulation. Results of numerical simulation are strongly dependent upon constitutive equations selected. In the present study the Carreau model, the modified Powell-Eyring model, the modified Cross model and the modified power-law model ar selected for numerical computation of steady tube flows. The centerline velocity in the developing region exhibits almost the same values regardless of the constitutive equation selected, but the ceterline velocity in the fully developed region shows slightly the effects of the selected constitutive equations. The Carreau model and the modified Cross model portrait almost the same values of the centerline velocity and pressure along the axis for different Reynolds number. The modified power-law model shows the Reynolds number dependence of the centerline velocity and pressure variation.

外國의 檢問制度와 人權保障

유상식(Yoo Sang-Sik) 한국비교공법학회 2003 공법학연구 Vol.4 No.2

난소적출 rat에 녹용, 홍삼, 홍화, ipriflavone 및 estrogen을 투여 했을 때 호르몬 Ca, P 및 ALP수준에 미치는 영향에 관한 연구

유상식 ( Sang Sik Yoo ),김민수 ( Min Soo Kim ),박상훈 ( Sang Hoon Park ),김상근 ( Sang Keun Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca and P deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600 mg/kg, p.o), red ginseng(200 mg/kg, p.o), safflower(200 mg/kg, po), ipriflavone(80 mg/kg, p.o) and estrogene(400,μg/kg, i,m) for 5 weeks. During the treatment, the rats were examined for serum concentrations of estracliol, calcitonin, Ca, P and alkaline phosphatase(ALP) activities. The results are summarized as follows 1. The levels of serum 17 /3 -estradiol after five weeks of treatment were showed 39.6 ± 3.0 pg/mi by antler, 33.2±2.5pg/ml by red ginseng, 34.9±2.4pg/ml by safflower, 28.1±3.1 pg/mi by ipriflavone and 40.6 ±3.0pg/ml by estrogen-treated group. They were lower than 50.8±3.lpg/ml of normal control group which had not received ovariectomy. They, however, were significantly higher than 26.8 + 1 .8pg/ml of ovariectomized non-treatment group(p<0.05). 2. The levels of serum calcitonin after five weeks of treatment were showed 0.60±0.02 ng/ml by antler, 0.55±0.04ng/ml by red ginseng, 0.59 ±0.02ng/ml by safflower, 0.56± 0.04ng/ml by ipriflavone and 0.62±0.02ng/ml by estrogen-treated group. They were lower than 0.67±0.03pg/ml of normal control group. However, they were significantly higher than 0.45 ± 0.05ng/ml of ovanectomized non-treatment group(p<0.05). 3. The levels of serum Ca of the rats after five weeks of treatment with antler, red ginseng, safflower, ipriflavone and estrogen were 23.51 ± 2.19μg/mℓ, 25.22 ± 3.44μg/mℓ, 23.20 ±4.02g/mℓ, 24.76 ±3.57μg/mℓ, 23.07 ±3.66μg/mℓ, respectively. They were a little higher than 21.43± 2.22μg/mℓ of normal control group. And non-treatment group showed 26.12 ±0.29μg/mℓ which was significantly higher than that of control group(p<0.05). 4. The serum P concentrations after five weeks of treatment were showed 12.11±2. 14μg/in by antler, 13.18 ± 1.64μg/mℓ by red ginseng, 12.67 ± 2.31μg/mℓ by safflower, 12.38 ± 2.07μg/mℓ by ipriflavone, 11.86±1.93μg/mℓ by estrogen-treated group. They were a bit higher than 11.29±1.23μg/mℓ of normal control group. And non-treatment group showed 13.42±1.87μg/mℓ which was higher than that of control group but not significant. 5. The levels of serum ALP after five weeks of treatment were showed 164.8±3.8IU/ml by antler, 277.7±4.8IU/ml by red ginseng, 288.5±4.51U/ml by safflower, 214.7 ±5.7IU/ml by ipriflavone and 159.4 ±5.4IU/ml by estrogen-treated group. They were significantly higher than 144.1 ±3.5IU/ml of normal control group(p<0.05). However, they were significantly lower than 336.9 ± 12.7IU/ml of ovariectomized non-treatment group(p<0.05). Antler and safflower elevated serum estradliol and calcitonin, and decreased serum ALP significantly. Therefore they were thought to have therapeutic effect on osteoposis by making inhibitory effect on osteoclasts rather than activating osteoblasts.

녹용, 홍삼, 홍화, ipriflavone 및 estrogen이 난소적출 rat의 체중, 골밀도, 장기중량 및 조직학적 변화에 관한 연구

유상식 ( Sang Sik Yoo ),김상근 ( Sang Keun Kim ),이명헌 ( Myung Heun Lee ),송운재 ( Woun Jae Song ),문병천 ( Byung Cheon Moon ),김무강 ( Moo Kang Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca and P deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600mg/kg, PO), red ginseng(200mg/kg, PO), safflower(200mg/kg, PO), ipriflavone(80mg/kg, PO) and estrogene(400μg/kg, IM) for 5 weeks. During the treatment, the rats were examined for body, organ, femur and femur ash weights. And electron-microscopical examinations were also performed on femora. The results are summarized as follows 1. The body weights of the antler, red ginseng, safflower, ipriflavone and estrogen-treated groups after five weeks of treatment, were 303.0±3.0g, 273.3±4.4g, 288.3±5.lg, 300.5± 5.2g, 320.5 ± 4.2g, respectively. They, except the red ginseng-treated group, were significantly higher than 278.4±2.7g of normal control group(P<0.05). And the body weight of non-treatment group was 294.2±5.5g which was higher than that of control group, too. 2. The weights of left femur after five weeks of treatment were showed 0.83 ± 0.2g by antler, 0.89±0.2g by red ginseng, 0.87±0.2g by safflower, 0.83±0.1g by ipriflavone and 0.78±0.lg by estrogen-treated group. They were a little lower than 0.97±0.lg of normal control group. Non-treatment group showed 0.83±0.lg which was lower than that of control group but not significant. 3. The weights of ashed left femora after five weeks of treatment were showed 0.36±0.01g by antler, 0.40 ± 0.01g by red ginseng, 0.41 ± 0.01g by safflower, 0.36 ± 0.01g by ipriflavone and 0.35 ± 0.01g by estrogen-treated group. Those results were significantly lower than 0.43 ±0.01g of normal control group. And non-treatment group showed 0.29 ±0.01g of femoral ash which was significantly lower than that showed by control group(p<0.05). 4. The femoral Ca contents of the antler, red ginseng, safflower, ipriflavone and estrogen-treated rats were 19.6±0.7%, 17.8 ± 1.1%, 18.8 ± 0.9%, 18.0±1.1%, 1 B.3 ± 1.1%, respectively, after five weeks of treatment. The femoral Ca contents of antler and safflower-treated groups were higher than that of control group which showed 18.2 ± 0.9% of Ca. The non-treatment group, showing 15.3±0.7%, had significantly lower content of Ca than normal group(p<0,05). 5. The weights of liver, spleen, and kidney in the osteoporosis-induced rats during the treatment with antler, red ginseng, safflower, ipriflavone and estrogen showed not significant changes. 6. The connections among the trabeculae of control group were maintained well, showing no bone loss. However, the connections among the trabeculae of non-treatment group were thinner than those of control group and were almost disconnected and the lacunae were found to be broadened. Antler and safflower-treated group showed only slight changes. The recovery rates were prominent in antler, safflower, estrogen-treated groups but they were insignificant in red ginseng and ipriflavone-treated groups.