난소적출 rat에 녹용, 홍삼, 홍화, ipriflavone 및 estrogen을 투여 했을 때 호르몬 Ca, P 및 ALP수준에 미치는 영향에 관한 연구

유상식 ( Sang Sik Yoo ),김민수 ( Min Soo Kim ),박상훈 ( Sang Hoon Park ),김상근 ( Sang Keun Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca and P deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600 mg/kg, p.o), red ginseng(200 mg/kg, p.o), safflower(200 mg/kg, po), ipriflavone(80 mg/kg, p.o) and estrogene(400,μg/kg, i,m) for 5 weeks. During the treatment, the rats were examined for serum concentrations of estracliol, calcitonin, Ca, P and alkaline phosphatase(ALP) activities. The results are summarized as follows 1. The levels of serum 17 /3 -estradiol after five weeks of treatment were showed 39.6 ± 3.0 pg/mi by antler, 33.2±2.5pg/ml by red ginseng, 34.9±2.4pg/ml by safflower, 28.1±3.1 pg/mi by ipriflavone and 40.6 ±3.0pg/ml by estrogen-treated group. They were lower than 50.8±3.lpg/ml of normal control group which had not received ovariectomy. They, however, were significantly higher than 26.8 + 1 .8pg/ml of ovariectomized non-treatment group(p<0.05). 2. The levels of serum calcitonin after five weeks of treatment were showed 0.60±0.02 ng/ml by antler, 0.55±0.04ng/ml by red ginseng, 0.59 ±0.02ng/ml by safflower, 0.56± 0.04ng/ml by ipriflavone and 0.62±0.02ng/ml by estrogen-treated group. They were lower than 0.67±0.03pg/ml of normal control group. However, they were significantly higher than 0.45 ± 0.05ng/ml of ovanectomized non-treatment group(p<0.05). 3. The levels of serum Ca of the rats after five weeks of treatment with antler, red ginseng, safflower, ipriflavone and estrogen were 23.51 ± 2.19μg/mℓ, 25.22 ± 3.44μg/mℓ, 23.20 ±4.02g/mℓ, 24.76 ±3.57μg/mℓ, 23.07 ±3.66μg/mℓ, respectively. They were a little higher than 21.43± 2.22μg/mℓ of normal control group. And non-treatment group showed 26.12 ±0.29μg/mℓ which was significantly higher than that of control group(p<0.05). 4. The serum P concentrations after five weeks of treatment were showed 12.11±2. 14μg/in by antler, 13.18 ± 1.64μg/mℓ by red ginseng, 12.67 ± 2.31μg/mℓ by safflower, 12.38 ± 2.07μg/mℓ by ipriflavone, 11.86±1.93μg/mℓ by estrogen-treated group. They were a bit higher than 11.29±1.23μg/mℓ of normal control group. And non-treatment group showed 13.42±1.87μg/mℓ which was higher than that of control group but not significant. 5. The levels of serum ALP after five weeks of treatment were showed 164.8±3.8IU/ml by antler, 277.7±4.8IU/ml by red ginseng, 288.5±4.51U/ml by safflower, 214.7 ±5.7IU/ml by ipriflavone and 159.4 ±5.4IU/ml by estrogen-treated group. They were significantly higher than 144.1 ±3.5IU/ml of normal control group(p<0.05). However, they were significantly lower than 336.9 ± 12.7IU/ml of ovariectomized non-treatment group(p<0.05). Antler and safflower elevated serum estradliol and calcitonin, and decreased serum ALP significantly. Therefore they were thought to have therapeutic effect on osteoposis by making inhibitory effect on osteoclasts rather than activating osteoblasts.

녹용, 홍삼, 홍화, ipriflavone 및 estrogen이 난소적출 rat의 체중, 골밀도, 장기중량 및 조직학적 변화에 관한 연구

유상식 ( Sang Sik Yoo ),김상근 ( Sang Keun Kim ),이명헌 ( Myung Heun Lee ),송운재 ( Woun Jae Song ),문병천 ( Byung Cheon Moon ),김무강 ( Moo Kang Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca and P deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600mg/kg, PO), red ginseng(200mg/kg, PO), safflower(200mg/kg, PO), ipriflavone(80mg/kg, PO) and estrogene(400μg/kg, IM) for 5 weeks. During the treatment, the rats were examined for body, organ, femur and femur ash weights. And electron-microscopical examinations were also performed on femora. The results are summarized as follows 1. The body weights of the antler, red ginseng, safflower, ipriflavone and estrogen-treated groups after five weeks of treatment, were 303.0±3.0g, 273.3±4.4g, 288.3±5.lg, 300.5± 5.2g, 320.5 ± 4.2g, respectively. They, except the red ginseng-treated group, were significantly higher than 278.4±2.7g of normal control group(P<0.05). And the body weight of non-treatment group was 294.2±5.5g which was higher than that of control group, too. 2. The weights of left femur after five weeks of treatment were showed 0.83 ± 0.2g by antler, 0.89±0.2g by red ginseng, 0.87±0.2g by safflower, 0.83±0.1g by ipriflavone and 0.78±0.lg by estrogen-treated group. They were a little lower than 0.97±0.lg of normal control group. Non-treatment group showed 0.83±0.lg which was lower than that of control group but not significant. 3. The weights of ashed left femora after five weeks of treatment were showed 0.36±0.01g by antler, 0.40 ± 0.01g by red ginseng, 0.41 ± 0.01g by safflower, 0.36 ± 0.01g by ipriflavone and 0.35 ± 0.01g by estrogen-treated group. Those results were significantly lower than 0.43 ±0.01g of normal control group. And non-treatment group showed 0.29 ±0.01g of femoral ash which was significantly lower than that showed by control group(p<0.05). 4. The femoral Ca contents of the antler, red ginseng, safflower, ipriflavone and estrogen-treated rats were 19.6±0.7%, 17.8 ± 1.1%, 18.8 ± 0.9%, 18.0±1.1%, 1 B.3 ± 1.1%, respectively, after five weeks of treatment. The femoral Ca contents of antler and safflower-treated groups were higher than that of control group which showed 18.2 ± 0.9% of Ca. The non-treatment group, showing 15.3±0.7%, had significantly lower content of Ca than normal group(p<0,05). 5. The weights of liver, spleen, and kidney in the osteoporosis-induced rats during the treatment with antler, red ginseng, safflower, ipriflavone and estrogen showed not significant changes. 6. The connections among the trabeculae of control group were maintained well, showing no bone loss. However, the connections among the trabeculae of non-treatment group were thinner than those of control group and were almost disconnected and the lacunae were found to be broadened. Antler and safflower-treated group showed only slight changes. The recovery rates were prominent in antler, safflower, estrogen-treated groups but they were insignificant in red ginseng and ipriflavone-treated groups.

外國의 檢問制度와 人權保障

유상식(Yoo Sang-Sik) 한국비교공법학회 2003 공법학연구 Vol.4 No.2

대전지역 길고양이의 톡소포자충 (Toxoplasma gondii) 감염 실태 조사

성선혜 ( Sun Hye Sung ),유상식 ( Sang Sik Yoo ),임여정 ( Yeo Jeong Im ),정년기 ( Nyun Ki Chung ),문병천 ( Byung Chun Moon ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.1

This study was performed to evaluate the prevalence rate of Toxoplasma gondii on 217 stray cats in Daejeon. The positive infection rate of T. gondii was 15.7% in enzyme-linked immunosorbent assay (ELISA), 12.4% in latex agglutination test (LAT), 14.7% in indirect immunofluorescent antibody test (IFA) and 0.5% in polymerase chain reaction (PCR) respectively. In districts, Yuseong-gu was shown the highest seropositive rate of T. gondii as 31.8% in ELISA, 22.7% in LAT and 31.8% in IFA. In gender, the seropositive rate of female cats was slightly higher than that of male cats as 17.2% in ELISA, 15.2% in LAT, 15.2% in IFA and 1.0% in PCR. Cats captured in National science museum, detached house and apartment was shown relatively high prevalence rate of T. gondii.

SefA 유전자 PCR에 의한 Salmonella serogroup D1의 특이적 검출

전무형,김태중,장경수,강경임,김귀현,김기석,유상식,김현수,신광순,김철중,Jun, Moo-hyung,Kim, Tae-joong,Chang, Kyung-soo,Kang, Kyong-im,Kim, Kui-hyun,Kim, Ki-seok,Yoo, Sang-sik,Kim, Hyun-soo,Shin, Kwang-soon,Kim, Chul-joong 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

Sal enteritidis thin fimbriae, SEF14, were found to be restricted to the predominantly poultry-associated members of the Salmonella serogroup D1 that are considered as the important pathogens in poultry industry. SefA together with sefB and sefC encode the proteins involved in SEF14 biosynthesis. In order to develop the rapid and specific detection methods for Salmonella serogroup D1, a PCR technique for the amplification of sefA gene was established, and its specificity and sensitivity were investigated with various microorganisms. The bacterial genomic DNA was extracted by colony-picking and rapid boiled-lysate technique. In comparison of Sef I and Sef II primers used in the PCR, Sef I primer for sefA gene of 513bp showed higher specificity than that of Sef II. The established PCR was as sensitive as to detect 1pg of Sal enteritidis DNA. When 73 strains in 28 genera including the reference strains and the field isolates of various Salmonella serotypes, Bacillus subtilis, Bordetella bronchisepdca, E coli, Listeria spp., Micrococcus luteus, Rhodococcus equi, Staphylococcus spp., Streptococcus spp., Vibrio parahemolyticus, Yersinia spp. were studied, the established PCR yielded specifically positive results with only Salmonella serogroup D1. The results suggested that the PCR for sefA gene could be a potential candidate among the specific detection methods for Salmonella serogroup D1.

대전 주택가 산책로 진드기의 인수공통전염병 병원체 감염실태 조사

한소영 ( So-young Han ),성선혜 ( Sun-hye Sung ),서진우 ( Jin-woo Seo ),김종호 ( Jong-ho Kim ),이석주 ( Seok-ju Lee ),유상식 ( Sang-sik Yoo ) 한국동물위생학회(구 한국가축위생학회) 2021 韓國家畜衛生學會誌 Vol.44 No.2

In this study, a total of 9,449 hard ticks were collected once a month from April to October 2020 from a neighborhood park in Daejeon by flagging & dragging method and CO2 manned trap method. The collected ticks were classified according to the Yamagutsi search table using a stereoscopic microscope and molecular biological analysis of four pathogens (SFTSV, Anaplasma spp., Ehrlichia spp., Borrellia spp.). As a result of the study, Haemaphysalis longicornis were collected the most in all areas of the five boroughs at a rate of 82 to 96 percent, while adults were collected the most in May to July, nymphs were collected the most in April to June, and larvae from August to October at a rate of 78 percent to 98 percent. In pathogens, three cases of SFTSV were detected, showing a minimum infection rate (MIR) of 0.46%, while Anaplasma spp. and Ehrlichia spp. were detected one each, with 0.15% and Borrelia spp. with a minimum infection rate of 0.46%. The detected SFTSV showed 99.9% homogeneity with the KF781490 detected in Cheongwon-gun, Chungbuk Province, Anaplasma spp. showed 99.0% homogeneity with JN990105 detected in China, and Erhlichia spp. showed 98.9% genetic similarity with U96436 separated from the U.S. In this study, the distribution status and pathogen infection rate of the hard ticks in the Daejeon area are analyzed and provided as basic data for the prevention of the hard tick-borne infectious disease.

난소적출 rat에 생약물질을 투여했을 때 호르몬 및 생화학치의 변동에 미치는 영향

유상식,김상근 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca- and P- deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600 ㎎/㎏, p.o), red ginseng(200 ㎎/㎏, p.o), safflower(200 ㎎/㎏, p.o), ipriflavone(80 ㎎/㎏, p.o) and estrogene(400 ㎍/㎏, i.m) for 5 weeks. During the treatment, the rats were examined for serum concentrations of estradiol, calcitonin, Ca, P, alkaline phosphatase(ALP) activities, body weight, femur ash and Ca contents. The results are summarized as follows: 1. The levels of serum 17 β-estradiol after five weeks of treatment were showed 39.6±3.0 pg/㎖ by antler-, 33.2±2.5 pg/㎖ by red ginseng-, 34.9±2.4 pg/㎖ by safflower-, 28.1±3.1 pg/㎖ by ipriflavone- and 40.6±3.0 pg/㎖ by estrogen-treated group. They, however, were signigicantly higher than 26.8±1.8 pg/㎖ of ovariectomized non-treatment group(p<0.05). 2. The levels of serum calcitonin after five weeks of treatment were showed 0.06±0.02 ng/㎖ by antler-, 0.55±0.04 ng/㎖ by red ginseng-, 0.59±0.02 ng/㎖ by safflower-, 0.56±0.04 ng/㎖ by ipriflavone- and 0.62±0.02 ng/㎖ by estrogen-treated group. However, they were significantly higher than 0.45±0.05 ng/㎖ of ovariectomized non-treatment group(p<0.05). 3. The levels of serum Ca contents after five weeks of treatment with antler, red ginseng, safflower, ipriflavone and estrogen were a little higher than 21.43±2.22 ㎍/㎖ of normal control group. 4. The serum P contents after five weeks of treatment were showed 12.11±2.14 ㎍/㎖ by antler-, 13.18±1.64 ㎍/㎖ by red ginseng-, 12.67±2.31 ㎍/㎖ by safflower-, 12.38±2.07 ㎍/㎖ by ipriflavone-, 11.86±1.93 ㎍/㎖ by estrogen-treated group. 5. The levels of serum ALP after five weeks of treatment were showed 164.8±3.8 IU/㎖ by antler-, 277.7±4.8 IU/㎖ by red ginseng-, 288.5±4.5 IU/㎖ by safflower-, 214.7±5.7 IU/㎖ by ipriflavone- and 159.4±5.4 IU/㎖ by estrogen-treated group. They were significantly higher than 144.1±3.5 IU/㎖ of normal control group(p<0.05). 6. The body weights of the antler-, red ginseng-, safflower-, ipriflavone- and estrogen-treated groups after five weeks of treatment, were 303.0±3.0g, 273.3±4.4g, 288.3±5.1g, 300.5±5.2g, 320.5±4.2g, respectively. 7. The weights of ashed left femora after five weeks of treatment were showed 0.36±0.01g by antler-, 0.40±0.01g by red ginseng-, 0.41±0.01g by safflower-, 0.36±0.01g by ipriflavone- and 0.35±0.01g by estrogen-treated group. Those results were significantly lower than 0.43±0.01g of normal control group. 8. The femoral Ca contents of the antler-, red ginseng-, safflower-, ipriflavone- and estrogen-treated rats were 19.6±0.7%, 17.8±1.1%, 18.8±0.9%, 18.0±1.1%, 18.3±1.1%, respectively, after five weeks of treatment. The femoral Ca contents of antler- and safflower-treated groups were higher than that of control group which showed 18.2±0.9% of Ca. Antler and safflower elevated serum estradiol and calcitonin, and decreased serum ALP significantly. Therefore they were thought to have therapeutic effect on osteoposis by making inhibitory effect on osteoclasts than activating osteoblasts.

한국 진도견의 serum protein 및 lipoprotein 변동에 관한 연구

유상식,이명헌,김상근 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The concentration of serum proteins and lipoproteins in Korean Jindo dogs were examined from the time of after birth to 150 days of age. Eight(4 females and 4 males) Jindo dogs were used in this study. Concentration of serum proteins and lipoproteins were fractioned by cellulose acetate electrophoresis. The results are summarized as follows; 1. The total serum protein concentrations at 1 days(4.6±0.4 g/dl) after birth were lower than in adult dogs, thereafter increased rapidly at 3 days(7.1±0.6 g/dl) of ages, and there was no significant changes between sexes. 2. The concentrations of serum protein from the time of after birth to 150 days of age were 0.35±0.1-8.5±0.2 g/dl and 0.5±0.1-0.8±0.2 g/dl, respectively. The γ-globulin concentrations were not detected 1 days after birth and increased rapidly after pups ingested colostrum(after 3 days of age), thereafter there was no significant changes of γ-globulin concentration after 10 days of age. 3. The concentrations of serum α, β-lipoprtein were not detected 1 days after birth and increased rapidly after pups ingested colostrum(at 3 days of age), from the time of after birth to 150 days of age were 49.1±11.6-94.1±16.4 ㎎/dl and 11.7±6.5-102.0±10.8 ㎎/dl, respectively. 4. the γ-lipoprtein concentrations no detected 1 days after birth and increased rapidly after pups ingested colostrum(at 3 days of age, 11.6±2.5 ㎎/dl) and decreased gradually during 50 days of age, and thereafter increased rapidly during 100-150 days of age(71.8±4.8-77.2±4.1 ㎎/dl).