가상 현실 신경학적 검사 교육 도구(VRNET) 버전 2.0을 의과대학 학생 교육에 적용한 경험

양시용,범진호,한은아,조준호 한국의료시뮬레이션학회 2023 한국의료시뮬레이션학회지 Vol.7 No.2

Background: In 2018, we developed a Virtual Reality Neurologic Examination Teaching tool (VRNET) to educate medical students on the neurological symptoms of the eye and face that could not be easily simulated by standardized patients (SP). VRNET 2.0, an enhanced version, facilitates neurological examinations of the arms and legs, thereby eliminating the need for SP and relying solely on the program for education. This study introduces VRNET 2.0 and reports its application in medical student education. Methods: This retrospective study analyzed the responses to a questionnaire administered to 362 fourth-year medical students after the completion of a practice run from March 2018 to October 2021. The questionnaire included questions on satisfaction, difficulty, appropriateness of time allocation, expectation, and achievement of learning goals. Results: Compared to the training method using SP + VRNET in 2019, training using VRNET 2.0 in 2021 was found to be superior in terms of satisfaction (8.18 [SP+VRNET] vs 8.81 [VRNET 2.0], p=.011) and difficulty (7.95 [SP+VRNET] vs 7.25 [VRNET 2.0], p=.040). Conclusion: Neurologic examination training using VRNET 2.0 for medical students showed higher satisfaction and lower difficulty than the training program combining SP and VRNET. Further research is essential to assess the actual educational effectiveness of VRNET 2.0.

유전자조작 , 균주분리 Phytate 분해 유산균의 분리 및 이용

양시용,송민동,김창원,유제현,정기철 한국미생물생명공학회 2001 한국미생물·생명공학회지 Vol.29 No.4

N/A

학술발표회 : 단위영양 ( 포스터발표 ) ; 방선균을 이용한 활생제 ( 생균제 ) 의 첨가가 육계의 생산성 및 환경오염 방지에 미치는 영향

양시용,김태규,오영균,서동준,김양숙,곽완섭,김창원,김중배 한국동물자원과학회 ( 구 한국축산학회 ) 1994 한국축산학회 심포지움자료집 Vol.- No.-

Phytic Acid와 Phytase에 관한 동물산업적 고찰

양시용,김창원,강창원 한국축산환경학회 2001 축산시설환경학회지 Vol.7 No.2

Phytic acid (myo-inositol hexaphosphate or IP6) is the major storage form of phosphorus in cereals and legumes, representing 18 to 88% of the total phosphorus. Phytate form of phosphorus is not readily utilized by monogastric animals and this result causes pollution problem by phosporus released in areas of intensive livestock production. The interaction between phytic acid and essential dietary minerals, protein, or vitamins is considered to be one of the primary factors limiting the nutritional values of cereals and legunes in monogastric animals. Attempts have been made to hydrolyze dietary phytic acid by phytases to improve the feed quality and to decrease the amount of phosphorus excreted by animals. Phytase(myo-inositol hexakisphosphate phosphohydrolase) hydrolyzes phytic acid to myo-inositol and phosphoric acid. Two types of phytases are known: 3-phytase (EC 3.1.3.8) and 6-phytase (EC 3.1.3.26), indicating the intial attack to the susceptable phosphoester bond. Because of its great industrial importance, there is ongoing interest in isolating new bacterial strains producing novel and efficient phytases.

꽃사슴의 단백질 요구량에 관한 연구

양시용 한국양록협회 1993 양록회보 Vol.18 No.-

본 시험은 3마리의 2년생 famale sika deer에게 동일한 에너지 수준하에 단백질 함량($7\%,\;12\%\;17\%$)이 각기 다른 사료를 급여하였을 때, 사료 영양소의 이용성 변화 및 체내 N 대사, 유지를 위한 단백질 요구량, 대사성 분질소 함량 등을 규명하기 위하여 실시하였으며, 결과를 요약하면 다음과 같다. 1. DM, OM, ADF의 소화율을 사료내 단백질 함량에 따른 차이를 보이지 않았으며(P>.1), CP의 소화율은 사료내 단백질 수준이 증가함에 따라 향상되었다(P<.05). 2. 사료내 단백질 함량이 증가함에 따라 분과 뇨로의 N 배출량 모두 증가하였으며(P<.05), 사료내 단백질 수준이 7, 12, $17\%$ 이었을 때 N retention은 각각 26.9, 31, 8, $38.9\%$였다. 3. 혈증 urea-N 함량은 사료내 단백질 함량의 증가에 따라 현저하게 증가하였다.(P<.05) 4. Famale sika deer의 유지수준의 단백질요구량(protein requirement for maintenance)은 $1.81g/kgW^{0.75}/d$다. 5. 대사성 분 질소(metabolic facal N)함량은 $0.32g/kgW^{0.75}/d$였다.

Probiotics용 복합효소 분비 Bacillus sp.의 분리 및 원료사료를 이용한 균주 생산을 위한 배지 조건의 최적화

양시용,송민동,김언현,김창원 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.2

가축 사료첨가용 probiotics용 균주를 얻기 위해 특히 내열성이 높아 가공사료에 적합한 Bacillus sp.를 위주로 하여 가축에게 유용한 효소인 phytase를 비롯하여 protease, cellulase, xylanase, amylase의 활성을 모두 나타내는 4-3 균주를 얻어 동정한 결과 Bacillus subtilis로 밝혀졌으며, 이를 B. subtilis 4-3으로 명명하였다. 본 균주를 원료사료에 순수배양하여 사료의 항영양인자인 phytic acid 분해율을 검토한 결과 대두박 및 쌀겨에 있어서는 phytic acid 분해율이 낮았으나, 밀기울의 경우 80.63%로 상대적으로 높은 phytic acid 분해율을 나타내었다. 원료 사료를 이용한 균주의 생산 조건은 대두박 1%(w/v)와 당밀 2%(w/v)가 가장 적합한 균주 생산을 위한 배지조성으로 검토되었다. Isolation of Bacillus sp. producing multi-enzyme and optimization of medium conditions for its production using feedstuffs for probiotics were carried out in this study. A bacterium isolated from natural resources, namely Bacillus subtilis 4-3, has multi-enzyme activity (phytase, cellulase, xylanase, protease, and amylase. In the culture of B. subtilis 4-3 using soybean meal and rice bran, relatively low phytate degradation was noted using whereas high phytate degradability was observed with wheat bran (80.63%). The optimal composition of medium using feedstuffs was 1.0% (w/v) soybean meal and 2% (w/v) molasses to yield high cell growth.

남은 음식물 발효를 위한 내염성 유산균의 분리

양시용,박홍양,김창원,박근규 한국축산환경학회 2001 축산시설환경학회지 Vol.7 No.2

The objective of this study was isolation of halotolerant lactic acid bacteria for fermentation of food wastes. 5 strains of lactic acid bacteria were isolated from fermented foods. Among isolated strains, the strain 5-2 was selected according to the growth characteristics in food wastes containing medium. The selected strain 5-2 was identified as Pediococcus acidilactici based on its biochemical characteristics.

엿밥의 조성 및 산유량과 유성분 조성에 미치는 영향

양시용,강창원,안규철,이강연,이중일,김정훈,박근규,Yang, Si-Yong,Kang, Chang-Won,Ahn, Gyu-Chul,Lee, Gang-Yeon,Lee, Jung-Il,Kim, Jeong-Hoon,Park, Keun-Kyu 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.3

This study was conducted to the evaluate nutritional value of corn taffy residue (CTR), a by-product from Korean traditional taffy, and to investigate the dietary effect of CTR on the yield and composition of milk from dairy cows. Relatively high concentrations of residual enzymes and sugars were found in CTR. Protein and fat concentrations were high, comparable to those of corn gluten meal. Inclusion of 1, 3, and 6% CTR in total mixed rations yielded more milk (p<0.05) than in the control group, and increased milk fat (p<0.05) in high-producing cows (approximately 30 kg). In low-producing cows, (approximately 20 kg), milk yield was increased by 1 and 6% CTR, and milk protein was enhanced by 3% CTR (p<0.05). Based on these results, utilization of CTR is recommended for production of high quality and economical animal products.

사료단백질 수준이 암컷 꽃사슴에 있어 질소대사에 미치는 영향

梁時容 건국대학교 1996 대학원 학술논문집 Vol.43 No.-

This study was conducted to evaluate the nutritional and physiological effect of dietary protein level on female sika deer; Throughout the experiment, each of 3 female sika deer was fed 3 diets isocalorically formulated at 7, 12 and 17% CP levels. Each of trial included 5-day transition, 10-day preliminary and 7-day collection periods. The results obtained were as follows; 1. Apparent digestibilities of CP, and ADF were affected by dietary protein level; however OM, and DM digestibilities were not affected by dietary protein level. 2. All the two-year old female sika deer at 7, 12 and 17% of dietary CP levels had positive nitrogen balance. 3. Blood urea nitrogen (BUN) was significantly greater (P<.05) in female sika deer with the dietary protein level increased. 4. Metabolic fecal nitrogen (MFN) estimated by regression analysis was 0.32 g/㎏W0.75/d. 5. Maintenance protein requirement estimated by regression analysis was 1.81 g/㎏W0.75/d.

Phytase 생산 균주의 분리 및 동정

양시용,배경숙,김창원,오태광,박근규 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

본 연구는 산업적으로 유용한 exo-type의 phytase 활성이 높은 균주의 선발 및 동정을 위해 실시하였다. 썩은 나무, 흙 등에서 분리한 1.500여 균주(곰팡이 700여 균주, 방선균 500여 균주, 세균 300여 균주)를 0.5% Ca-phytate를 함유한 phytase screening 배지를 이용하여 1차적으로 phytase 활성을 나타내는 균주를 선발한 결과 300여 균주를 얻었으며, 2차로 Na-phytate를 기질로 하여 exo-type의 phytase 활성을 측정한 결과 0.1 units/ml 이상의 높은 phytase 활성을 나타내는 곰팡이 5균주를 얻었으며, 이중 가장 phytase 활성 및 specific activity가 높은 No. 343 균주를 최종적으로 선발하여 형태학적 특징을 관찰하여 동정한 결과 Penicillium waksmanii로 동정되었다. 5 L jar fermenter(2 vvm, 500 rpm)를 이용하여 YM 배지에서 28℃ 배양조건으로 배양한 결과 96 시간 경과시 가장 높은 phytase 활성을 나타내었으며, 이때 배양액의 pH는 3.2∼3.5로 나타났다. Phytase (myo-inositol hexakisphosphate phosphohydrolase; EC 3.1,3.8) catalyzes the hydrolysis of myo-inositol hexakisphosphate, commonly known as phytic acid, that is pricipal storage form of phosphorus in seeds and pollen. The phytase-producing microorganisms occurring on rotten woods and soils were selectively isolated on the phytase screening medium with 0.5% Ca-phytate. Among more than three-hundred isolated of phytase-producing microorganisms, one fungal isolate was selected, having the highest exo-phytase production. The exo-phytase producer, fungal isolate No. 343 was identified as a strain of Penicillium waksmanii on the basis of morphological characteristics. Maximum production of phytase was reached after 96 hr of cultivation with aeration of 2 vvm in a 5 L jar fermenter.