광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 Infection and Chemotherapy Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwangju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics. Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwangju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

황련해독탕 추출액의 각막염 원인균에 대한 항균효과

김인수,서진종,김광곤,하동룡,신민구,김의수,전상윤,Kim, In-Soo,Seo, Jin-Jong,Kim, Kwang-Gon,Ha, Dong-Ryong,Shin, Min-Koo,Kim, Eui-Su,Jeon, Sang-Yun 대한한방내과학회 2014 大韓韓方內科學會誌 Vol.35 No.3

Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of $70{\mu}l$ dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from $70{\mu}l$ to $10{\mu}l$ at the same concentration. Results: 1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show any antimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentration but didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract $70{\mu}l$ was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.

광주지역 HIV/AIDS, 매독 혈청 양성률 및 임질 양성률 분포조사(2002-2006)

기혜영,서진종,김선희,김민지,김은선,정재근,최병선,박종태 대한감염학회 2009 Infection and Chemotherapy Vol.41 No.4

Background:The epidemic of human immunodeficiency virus (HIV) or sexually transmitted infections (STIs) is an important public health issue in the worldwide. Therefore, we investigated the trends of the seroprevalence of HIV and, syphilis and the gonorrhea positive rates in Gwangju from 2002 to 2006. Materials and Methods:The results of laboratory tests for HIV, syphilis and gonorrhea were collected from five public health centers and 19 hospitals in Gwangju. The number of tested patients from 2002 to 2006 was 348,202 cases for HIV, 80,115 cases for syphilis and, 103,548 cases for gonorrhea. Results:HIV/AIDS infections have rapidly increased at public health centers and hospitals. Especially, most of newly HIV-infected people were patients on ambulatory care/hospitalization, and they were also found via pre- operation tests and according to the clinical symptoms. The gonorrhea positive rates have gradually decreased by year while the syphilis infection rates have slightly increased from 2002 to 2006. Conclusions:We have to determine the reasons why HIV and syphilis infections, but not gonorrhea, have increases simultaneously by the year. These results should be helpful to create a more effective STD prevention policy and the treatment guidelines for the people in Gwangju in the near future. Background:The epidemic of human immunodeficiency virus (HIV) or sexually transmitted infections (STIs) is an important public health issue in the worldwide. Therefore, we investigated the trends of the seroprevalence of HIV and, syphilis and the gonorrhea positive rates in Gwangju from 2002 to 2006. Materials and Methods:The results of laboratory tests for HIV, syphilis and gonorrhea were collected from five public health centers and 19 hospitals in Gwangju. The number of tested patients from 2002 to 2006 was 348,202 cases for HIV, 80,115 cases for syphilis and, 103,548 cases for gonorrhea. Results:HIV/AIDS infections have rapidly increased at public health centers and hospitals. Especially, most of newly HIV-infected people were patients on ambulatory care/hospitalization, and they were also found via pre- operation tests and according to the clinical symptoms. The gonorrhea positive rates have gradually decreased by year while the syphilis infection rates have slightly increased from 2002 to 2006. Conclusions:We have to determine the reasons why HIV and syphilis infections, but not gonorrhea, have increases simultaneously by the year. These results should be helpful to create a more effective STD prevention policy and the treatment guidelines for the people in Gwangju in the near future.

광주지역 HIV/AIDS, 매독 혈청 양성률 및 임질 양성률 분포조사(2002-2006)

기혜영,최병선,서진종,김선희,김민지,김은선,박종태,정재근 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Background : The epidemic of human immunodeficiency virus (HIV) or sexually transmitted infections (STIs) is an important public health issue in the worldwide. Therefore, we investigated the trends of the seroprevalence of HIV and, syphilis and the gonorrhea positive rates in Gwangju from 2002 to 2006. Materials and Methods : The results of laboratory tests for HIV, syphilis and gonorrhea were collected from five public health centers and 19 hospitals in Gwangju. The number of tested patients from 2002 to 2006 was 348,202 cases for HIV, 80,115 cases for syphilis and, 103,548 cases for gonorrhea. Results : HIV/AIDS infections have rapidly increased at public health centers and hospitals. Especially, most of newly HIV-infected people were patients on ambulatory care/hospitalization, and they were also found via preoperation tests and according to the clinical symptoms. The gonorrhea positive rates have gradually decreased by year while the syphilis infection rates have slightly increased from 2002 to 2006. Conclusions : We have to determine the reasons why HIV and syphilis infections, but not gonorrhea, have increases simultaneously by the year. These results should be helpful to create a more effective STD prevention policy and the treatment guidelines for the people in Gwangju in the near future.

Shigella flexneri에 의한 집단발병의 역학적 양상

기현균,김선희,기혜영,서진종,하동룡,김은선,정재근,김성한,이복권 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

배경 : 국내에서 Shigella flexneri에 의한 감염으로 인한 집단발병은 2000년도에 국립보건원에 집단발병례가 보고된 이후 보고되지 않았으나 최근 저자 등은 S. flexneri에 의한 대규모 집단발병을 경험하여 역학적인 양상을 기술하였다. 방법 : OO회사의 직원 및 구내식당을 이용한 사람 및 가족들을 대상으로 증상여부 및 2002년 12월 23일 이후 식당이용 여부와 취식음식에 대하여 설문조사를 실시하였으며 검체를 확보하여 미생물학적 검사를 실시하였다. 결과 : 조사대상 총 258명 중 총 110명에서 증상이 발생하였으며 증상발생일의 분포는 단일정점을 보였다. 확진자는 82명으로 이중 81명은 OO의 식당을 이용한 사람이었고 1명은 식당에서 직접 조리과정에 참여한 사람이었다. 식사여부와 증상여부의 관계를 분석한 결과 특히 24일의 취식여부의 상대위험도가 가장 높았다(RR=6.46, 3.56≤95% CI≤11.75). 항균제 내성 양상은 ampicillin 내성균주가 82균주(100.0%) ampicillin-sulbactam 내성균주가 55균주(67.1%), amoxicillin-clavulanic acid에 대한 내성균주가 62균주(74.4%), trimethoprim-sulfamethoxazole 내성균주가 82균주로 나타났다(98.8%). 분리된 균주간의 연관성을 보기 위하여 실시한 PFGE 결과 본 집단발병에서 확인된 균주는 동일한 양상을 보였으나 비슷한 시기에 분리된 균주와는 서로 다른 양상을 보였다. 결론 : 본 집단발병은 국내에서 발생한 S. flexneri에 의한 집단발병 중 가장 대규모 집단발병이었다. 본 집단발병에서 확인된 균주간의 PFGE양상이 동일하였으나 기존에 국내에서 분리된 균주의 PFGE양상과는 다른 양상을 보였다. 따라서 이러한 상황을 감안한다면 S. flexneri 및 설사환자에 대한 감시체계를 강화해야 할 것이다. Background : Most of the shigellosis outbreak in Korea was caused by Shigella sonnei since late 1990's. In contrast, outbreak by Shigella flexneri were rare since late 1990's. Currently. we experienced the shigellosis outbreak by S. flexneri and described the results of investigation. Methods : We recruited employees from company "A" who had a meal at least once at the company's cafeteria from Dec 23th, 2002 to Dec 26th, 2002. We surveyed the symptoms, food items, and history of travel of eligible persons and their family members. For the microbiological examination, we collected specimen from eligible persons and their family. Collected specimens were cultured for bacteriologic agents and viruses. Epidemiological relationship among the isolates were analyzed by pulsed field gel electrophoresis (PFGE). Results : Among the eligible persons, one hundred ten were symptomatic (110/258, 42,6%). Eighty-two were confirmed bacteriologically. Day of symptom onset showed a unipolar pattern. Diarrhea was the most common symptom among the symptomatic patients (110/110, 100%). The other symptoms included abdominal pain (81.8%), tenesmus (70.0%), headache (63.6%), nausea (61.8%), febrile sense (59.1%), and vomiting (24.5%). Lunch on 24th Dec was the most significant risk factor of the outbreak (RR=6.46, 3.56≤95% Cl≤11.75). All isolates of the outbreak has the same pattern on PFGE analysis and the PFGE pattern was not similar compared with other S. flexneri isolates from Korea. Conclusion : This is the largest and unique outbreak by S.flexneri since 2000 in Korea. The outbreak has the same origin according to the PFGE pattern and epidemic characteristics. Considering rarity of S.flexneri among shigellosis in Korea, surveillance for S.flexneri should be enhanced.

황금탕 및 황금추출물의 식중독 세균들에 대한 생육억제 효과

이종기(Chong Ki Lee),서진종(Jin Jong Seo) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.10

각종 식중독의 원인균으로 중요시 되는 11종의 균주에 대해서 황금탕 및 황금 추출물의 항균력을 검토한 결과, 황금탕 추출물은 Staphylococcus aureus, Bacillus cereus, Shigella sonnei, Shigella flexneri, Salmonella Typhimurium, Salmonella Enteritidis, Vibrio parahaemolyticus, Escherichia coli O111 및 Escherichia coli O126에서 항균력을 나타내었고, 황금 추출물은 Staphylococcus aureus, Bacillus cereus, Shigella sonnei, Shigella flexneri, Salmonella Typhimurium, Salmonella Enteritidis, Vibrio parahaemolyticus, Escherichia coli O111, Escherichia coli O126 및 Escherichia coli O157에서 항균력을 나타내었다. Listeria monocytogenes에 대해서는 황금탕 및 황금 추출물 모두 항균력이 없었고, Escherichia coli O157에 대해서는 황금탕 추출물은 항균력이 없었으나, 황금 추출물은 항균력을 나타내었다. 황금탕 추출물의 최소저해농도는 Staphylococcus aureus와 Bacillus cereus에서 40 ㎎/mL, Shigella flexneri와 Salmonella Enteritidis에서 100 ㎎/mL이었고, 황금 추출물의 최소저해농도는 Bacillus cereus에서 10 ㎎/mL, Staphylococcus aureus, Shigella flexneri 및 Salmonella Enteritidis에서 20 ㎎/mL이었으며, 황금탕 및 황금 추출물 모두 Staphylococcus aureus와 Bacillus cereus에 대해 48시간까지 배양하는 동안 최소저해농도 이상에서는 균이 거의 생육하지 못하였다. 이상에서 황금탕 추출물보다는 황금 추출물의 항균력이 더 강하며, 황금탕 추출물의 식중독 세균들에 대한 항균력은 황금에 의한 것으로 생각된다. 또한, 항균력이 확인된 세균으로 인한 각종 식중독에 황금탕의 사용가능성을 고려할 만하며, 황금 추출물에 대한 천연 식품 보존료로서의 이용 가능성을 검토할 가치가 있다고 사료된다. The effects of Whangkumtang extract and Scu1tellariae radix extract on the microbial growth were investigated. The antimicrobial activities and cell growth inhibiting effects were investigated to selected strains with different concentrations of Whangkumtang and Scutellariae radix extracts. Whangkumtang and Scutellariae radix extracts showed the antimicrobial activities on Staphylococcus aureus, Bacillus cereus, Shigella sonnei, Shigella flexneri, Salmonella Typhimurium, Salmonella Enteritidis, Vibrio parahaemolyticus, Escherichia coli O111 and Escherichia coli O126. Whangkumtang and Scutellariae radix extracts did not show the antimicrobial activity on Listeria monocytogenes. Scutellariae radix extract showed the antimicrobial activity on Escherichia coli O157 but Whangkumtang extract did not. Minimum inhibitory concentrations (MIC) of Whangkumtang extract for each strain appeared to 40 ㎎/mL on Staphylococcus aureus and Bacillus cereus, 100 ㎎/mL on Shigella Flexneri and Salmonella enteritidis. The MICs of Scutellariae radix extract appeared to 10 ㎎/mL on Bacillus cereus, 20 ㎎/mL on Staphylococcus aureus, Shigella flexneri and Salmonella Enteritidis. Scutellariae radix extract showed the higher antimicrobial activity than Whangkumtang extract. The cell growth inhibitions by Whangkumtang and Scutellariae radix extracts were observed from Staphylococcus aureus and Bacillus cereus, repectively, during 48-hr incubation period.

두 개 학교에서 동시에 발생한 SRSV 감염증의 역학적 양상 및 바이러스의 유전적 양상

기현균,정영화,정재근,서진종,김선희,기혜영,천두성,지영미,하동룡 대한감염학회 2003 감염과 화학요법 Vol.35 No.2

배경 : SRSV는 바이러스에 의한 설사의 주요 원인병원체이다. 그러나 SRSV가 집단 식중독의 원인 병원체로 발견된 경우는 많지 않으며 특히 일정한 지역사회 내에서의 바이러스의 유전적인 다양성에 대한 연구는 없는 상황이다. 이러한 점에 착안하여 저자 등은 2개 학교에서 같은 시기에 동시에 발생한 SRSV 식중독의 역학적인 양상을 기술하며 식중독에서 나타난 SRSV의 유전학적인 변이를 기술하고자 한다. 방법 : 학교에서의 조사를 위하여 집단 식중독이 발생한 두 학교의 학생을 면접하여 인구학적 자료, 증상여부 및 증상의 종류, 섭취한 음식의 종류 등에 대하여 조사하였다. 미생물학적 검사를 위하여 4종의 세균과(Shigella spp. Salmonella spp. E. coli O157 : H7) 3종의 바이러스에 대하여 검사를 실시하였다(Norwalk agent, SRSV, adenovirus, and astrovirus). 결과 : 조사결과 두 학교에서의 증상자 수는 193명이었으며(193/2,843. 6.8%) 환자 증상일의 분포곡선은 단일정점을 보이는 양상이었다. 나타난 증상으로는 복통(176/193, 91.2%), 두통(111/193, 57.5%), 구토(102/193, 52.8%), 설사(83/193, 43.0%), 열감(79/193, 40.9%), 오심(73/193, 37.8%), 오한(49/193, 25.4%), 후중기의 순이었다(8/193, 4.1%). 실험실 검사결과 9건의 검체에서 SRSV가 PCR로 확인되었으며 RNA polymerase에 대한 DNA sequencing 결과 두 학교에서 각각 2개의 genogroup (Genogroup Ⅰ, Ⅱ)과 3개의 genotype이 발견되었다. 결론 : 본 연구결과 두 학교에서 동시에 발생한 SRSV에 의한 집단 감염증을 확인하였으며 3개의 다른 genotype을 각각의 학교에서 확인하였다. 본 연구의 의의는 같은 시기에 집단으로 발생한 SRSV 감염증이라도 서로 다른 유전형이 발견될 수 있음을 제시하였으며 추후 지역사회 및 국내에서의 SRSV 감염증에서 발견된 유전형과의 차이에 대한 연구가 진행되어야 함을 시사하는데 있다. Background: Small round structured virus (SRSV) is one of the common etiologic agents of viral gastroenteritis in childhood. However, SRSV has not easily been detected in many diarrheal outbreaks. Therefore, we experienced simultaneous outbreak of SRSV enteritis in two elementary schools, therefore, we analyzed the result of the surveillance. Methods: We interviewed the pupil of two schools and questioned their demographic data, presence of gastrointestinal symptoms, contact with symptomatic persons within the last two weeks, and dietary history within the last one week in school. The specimens of the symptomatic pupil were collected for identification of bacteria (Shigella spp, Salmonella spp, E.coli O157:H7) and viruses (Norwalk agent, SRSV, adenovirus, and astrovirus). Results: The number of symptomatic patients was 193 (193/2843, 6.8%) and the distribution of symptom onset was unipolar. Frequent symptoms of the patients were abdominal pain (176/193, 91.2%), headache (111/193, 57.5%), vomiting (102/193, 52.8%), diarrhea (83/ 193, 43.0%), febrile sense (79/193, 40.9%), nausea (73/193, 37.8%), chilling (49/193, 25.4%), and tenesmus (8/193, 4.1%). We identified SRSV in 9 cases by PCR method, and analyzed the RNA polymerase gene by DNA sequencing; 2 genogroup (Genogroup Ⅰ, Ⅱ) and 3 genotype. Conclusion: We confirmed SRSV enteritis with different genotypes in two concurrent outbreaks. DNA sequencing and comparison of genotype among the isolated SRSV in near future are be necessary

2000년-2009년 광주 광역시 지역의 급성설사환자의 분변에서 분리한 살모넬라 균주에서의 항생제 내성률 조사

김태순,김민지,김선희,서진종,기혜영,정재근,김은선,문용운,하동룡,김민경,임숙경,남향미,Kim, Tae Sun,Kim, Min Ji,Kim, Sun Hee,Seo, Jin-Jong,Kee, Hye Young,Chung, Jae Keun,Kim, Eun Sun,Moon, Yong Woon,Ha, Dong Ryong,Kim, Min Kyeong,Lim, Suk The Microbiological Society of Korea 2013 미생물학회지 Vol.49 No.2

지난 2000년부터 2009년까지 10년간 광주광역시 지역의 급성설사환자로부터 분리한 총 596개의 살모넬라균주에 대하여 총 16종의 항생제에 대한 감수성 검사를 실시한 결과, 내성빈도가 가장 높았던 약제는 ampicillin (43%), tetracycline (35.9%), nalidixic acid (31.5%), 그리고 chloramphenicol (26.2%) 순으로 나타났다. 혈청형 간에 항생제 내성률에 차이가 있었는데 S. Enteritidis와 S. Typhimurium이 각각 가장 높은 내성률을 보인 약제는 ampicillin (51.1%)과 tetracycline (77.9%)이었다. 총 89가지 내성패턴이 관찰되었으며, 26% (155/596)의 살모넬라 분리주는 검사한 16가지 약제에 모두 감수성을 보였다. 약 21% (127/596) 및 15% (87/596)의 분리주는 각각 한 개 및 두 개의 약제에 내성을 나타냈다. 그 외의 살모넬라 분리주(227/596, 38%)는 세가지 이상의 약제에 내성을 나타냈다(다제내성균). 다제내성균이 가장 높은 비율로 나타난 혈청형은 S. Paratyphi B (76.5%), S. Typhimurium (58.2%), 그리고 S. Enteritidis (40.2%)의 순이었다. 가장 흔한 다제내성패턴은 ampicillin-chloramphenicol-nalidixic acid-ticarcillin (36/227, 15.8%)이었으며 이러한 패턴을 보였던 다제내성균의 대부분(35/36, 97.2%)이 S. Enteritidis이었다. Antibiotic susceptibility was examined for 596 Salmonella isolates from patients with acute gastroenteritis during 2000-2009 in Gwangju area in South Korea. Of 16 antibiotics tested, ampicillin resistance (43%) was the most commonly observed resistance among the 596 Salmonella sp. isolates, followed by tetracycline (35.9%), nalidixic acid (31.5%), and chloramphenicol (26.2%). Antibiotic resistance varied among serotypes: The highest resistance of S. Enteritidis and S. Typhimurium was to ampicillin (51.1%) and tetracycline (77.9%), respectively. A total of 89 resistance patterns were observed, and 26% (155/596) of Salmonella isolates were susceptible to all antibiotics tested in this study. About 21% (127/596) and 15% (87/596) of the isolates were resistant to one and two antibiotics, respectively. The rest of Salmonella isolates (227/596, 38%) were resistant to three or more antibiotic agents. The highest multi-drug resistance (MDR) was observed in serotype S. Paratyphi B (76.5%), followed by S. Typhimurium (58.2%), and S. Enteritidis (40.2%). The most common resistance pattern of MDR isolates was ampicillin-chloramphenicol-nalidixic acid-ticarcillin (36/227, 15.8%), most of which (35/36, 97.2%) were S. Enteritidis.

인진호 추출물의 식중독 세균들에 대한 생육억제 효과

이종기(Chong-Ki Lee),서진종(Jin-Jong Seo) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.8

천연 보존료의 개발을 위한 식품학적 기초자료로 활용하기 위해 인진호 추출물을 이용하여 9종의 병원균과 식중독균 Salmonella typhimurium에서는 80 mg/mL로 나타났으며, 측정된 48시간 동안까지 최소저해농도 이상에서는 균이 거의 생육하지 못하였다. 인진호 아세톤 추출물의 순차 분획물의 항균활성은 에틸아세테이트와 부탄올 분획물이 Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Vibrio parahaemolyticus에서, 클로로포름 분획물은 Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium에서 항균활성을 나내었으며, 헥산 분획물은 Bacillus subtilis에서만 항균활성을 나타내었다. 이같은 사실은 인진호 추출물의 항균성 물질은 특정 용매에만 용해되지 않고 다른 용매에도 용해되는 성분으로 단일 물질보다는 여러 성분일 것으로 생각된다. 이상의 결과에서 천연물에 따라 항균력을 가지는 물질이 다양하여 추출용매의 선정이 항균성을 나타내는 성분을 찾아내는데 중요하다고 생각되며, 또한 인진호에서 추출된 항균성 물질이 Staphylococcus aureus나 Vibrio parahaemolyticus 등 식중독균의 생육억제에 효과가 있을 것으로 사료된다. The solvent extracts of the aerial part of Artemisia capillaris extracted by using several solvents with different polarities were prepared and their antimicrobial activities were examined. The antimicrobial activities and cell growth inhibitions were investigated to each strain with the different concentrations of the aerial part of Artemisia capillaris. Acetone extract showed the highest antimicrobial activity. Minimum inhibitory concentrations (MIC) for each strain were appeared to 20 mg/mL at Staphylococcus aureus and Bacillus subtilis, 40 mg/mL at Vibrio parahaemolyticus, and 80 mg/mL at Salmonella typhimurium. The cell growth inhibitions were shown on Staphylococcus aureus, Bacillus subtilis, Vibrio parahaemolyticus, and Salmonella typhimurium for 48 hours. The acetone extract was further fractionated sequentially with hexane, chloroform, ethyl acetate, and butanol for purifying crude acetone extract. The solvent fraction of hexane, chloroform, ethyl acetate, and butanol showed the different antimicrobial activity, respectively.