식중독 원인 미생물 장기 보존 방법에 대한 고찰

김유진,김수민,김소연,윤요한,Kim, Yujin,Kim, Soomin,Kim, Soyeon,Yoon, Yohan 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.3

The rapid development of biotechnology has increased the importance of microorganisms or their genetic information. Thus, the Nagoya Protocol on access to genetic resources and the fair and equitable sharing of benefits arising from their utilization was established, and countries are working to secure industrially and academically useful bioresources to deal with the agreement. In the case of Korea, because 67% of bioresources are imported from abroad, we are required to secure domestic bioresources as well. The number of isolated foodborne illness-causing microorganisms is predicted to invrease based on the incresing number of outbreaks of foodborne illness each year. Consequently, appropriate long-term preservation methods are necessary to secure the isolated microorganisms for the purpose of research and resourcification. Therefore, the long-term preservation methods for bacteria, fungi, viruses, and protozoa were investigated in this study, from domestic and international bioresource banks, and the functions of the cryoprotectants were reviewed and discussed. This review should be informative in the preservation of microorganisms and contribute to the development of biotechnology.

인식 단위로서의 한국어 음절에 대한 연구

김유진,김회린,정재호,Kim, Yu-Jin,Kim, Hoi-Rin,Chung, Jae-Ho 한국음향학회 1997 韓國音響學會誌 Vol.16 No.3

본 논문에서는 한국어 대용량 어휘 인식 시스템에 적합한 인식 단위에 대하여 연구 및 실험하였다. 특히 현재 인식 시스템의 인식 단위로 주로 사용되는 음소와 한국어의 특징을 잘 나타내는 음절을 선택하고, 인식 실험을 통해 음절이 한국어 인식 시스템의 인식 단위로서 적합한가를 음소와 비교하였다. 객관적인 비교 인식 실험 결과를 제시하기 위하여 동일한 남성 화자의 음성 데이터를 수집하고, 수작업 음소 경계 및 레이블링 과정을 거친 음성 데이터 베이스를 구축하였다. 또한 각 인식 단위에 동일한 HMM 기반의 훈련 및 인식 알고리즘을 적용하기 위해 Entropic사의 HTK (HMM Tool Kit) 2.0을 사용하였다. 각 인식 단위의 훈련을 위해 5상태 3출력, 8상태 6출력 HMM 모델의 연속 HMM (Continuous HMM)을 적용하였고, PBW 3회분, POW 1회분을 훈련에 사용하고 PBW 1회분을 각 인식 단위로서 인식하는 화자 종속 단어 인식 실험을 구성하였다. 실험 결과 8상태 6출력 모델을 사용한 경우 음소 단위는 95.65%, 음절 단위는 94.41%의 인식률을 나타내었다. 한편 인식 속도에서는 음절이 음소보다 약 25% 빠른 것으로 나타났다. In this paper, study and experiments are performed for finding recognition unit fit which can be used in large vocabulary recognition system. Specifically, a phoneme that is currently used as recognition unit and a syllable in which Korean is well characterized are selected. From comparisons of recognition experiments, the study is performed whether a syllable can be considered as recognition unit of Korean recognition system. For report of an objective result of the comparison experiment, we collected speech data of a male speaker and processed them by hand-segmentation for phoneme boundary and labeling to construct speech database. And for training and recognition based on HMM, we used HTK (HMM Tool Kit) 2.0 of commercial tool from Entropic Co. to experiment in same condition. We applied two HMM model topologies, 3 emitting state of 5 state and 6 emitting state of 8 state, in Continuous HMM on training of each recognition unit. We also used 3 sets of PBW (Phonetically Balanced Words) and 1 set of POW(Phonetically Optimized Words) for training and another 1 set of PBW for recognition, that is "Speaker Dependent Medium Vocabulary Size Recognition." Experiments result reports that recognition rate is 95.65% in phoneme unit, 94.41% in syllable unit and decoding time of recognition in syllable unit is faster by 25% than in phoneme.

Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles

김유진,Bum Chul Park,Young Soo Choi,고민준,Young Keun Kim 대한금속·재료학회 2019 ELECTRONIC MATERIALS LETTERS Vol.15 No.4

Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticlesin therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) arewidely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging,magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis ofthe cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze theintracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change accordingto their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, andMDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effectof nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules.

초음속 충돌 제트 운동에 대한 k-$\omega$ 난류모델의 적용

김유진,박수형,권장혁,김성인,박승오,이광섭,홍승규,Kim E.,Park S. H.,Kwon J. H.,Kim S. I.,Park S. O.,Lee K. S.,Hong S. G. 한국전산유체공학회 2004 한국전산유체공학회지 Vol.9 No.2

A numerical study of underexpanded jet and impingement on a wall mounted at various distances from the nozzle exit is presented. The 3-dimensional Wavier-Stokes equations and κ-ω turbulence equations are solved. The grids are constructed as overlapped grid systems to examine the distance effect. The DADI method is applied to obtain steady-state solutions. To avoid numerical instability such as the carbuncle phenomena that sometimes accompany approximate Riemann solver, the HLLE+ scheme is employed for the inviscid flux at the cell interfaces. A goal of this work is to apply a number of two-equation turbulence models based on the w equation to the impinging jet problem.

Collaborative Planning Model for Brownfield Regeneration

김유진,패트릭 밀러,Kim, Eujin Julia,Miller, Patrick Korean Institute of Landscape Architecture 2015 韓國造景學會誌 Vol.43 No.3

Unlike most other planning processes, brownfield planning generally requires a high level of technical and legal expertise due to potential site contamination. To successfully engage in inclusionary decision making, an adaptive collaboration strategy for brownfield planning is therefore critical. This study examines how a communicative planning approach can be used to overcome the challenge of enabling experts from different fields to work alongside lay people from the local community to achieve a properly balanced collaboration in brownfield planning. After identifying appropriate indicators for collaboration through a literature review of established communicative planning theory, these indicators are applied to the brownfield planning process, highlighting critical points of collaboration such as site prioritization, assessment, remediation, and redevelopment throughout. The results suggest the critical need for an adaptive model focusing on three aspects: 1. Facilitation of a balanced dialogue between the experts with social, cultural, and design-based knowledge and the ones with scientific and engineering-based knowledge, 2. Preparation of an appropriate tool for risk communication with the lay people, 3. Development of decision support system for the integration of expert-oriented technical data and public opinion-oriented subjective data. 대부분의 일반적인 계획 과정과 달리, 브라운필드 계획은 잠재된 오염문제로 인한 기술적, 법률적 전문지식을 필요로 한다. 성공적으로 통합적인 의사결정에 관여하기 위해서는 조정된 협력적 계획 모델이 중요하다. 본 연구는 기존의 협력적 계획 접근방식이 브라운 필드 재생에 있어 상반된 분야의 전문가들 및 지역 주민들이 함께 일하며 적절하게 균형 잡힌 협력을 이루어 내기 위한 어려움들을 극복하는 데에 어떻게 효과적으로 적용될 수 있는지 알아보는 것을 목표로 한다. 의사소통적 계획이론 관련 문헌분석을 통해 적절한 지표들을 찾아내어, 네 단계의 브라운 필드 계획과정(대상지 선별, 평가, 정화, 디자인)에 적용시켜 본 후, 중요한 부분들을 짚어내었다. 본 연구의 결과, 크게 세 가지 점에 초점을 맞춘, 브라운 필드 재생에 적합한 협력적 계획 모델의 필요성이 인식되었다(1. 사회, 문화, 디자인 분야와 과학, 기술 분야의 전문가 간의 균형 잡힌 대화의 촉진, 2. 일반주민들과의 잠재적 오염 위험과 관련한 커뮤니케이션을 위한 적절한 방법개발, 3. 전문가 주도의 객관적 데이터와 주민 의견 중심의 주관적 데이터의 통합을 위한 의사결정지원 시스템의 마련).

하수관거 모니터링 프로그램(SM5-PLUS) 소개

김유진,오정환,박해경,Kim, Yu-Jin,O, Jeong-Hwan,Park, Hae-Gyeong 한국수자원학회 2012 물과 미래(한국수자원학회지) Vol.45 No.10

제1형 당뇨 환아를 위한 에듀테인먼트 콘텐츠 제안

김유진,김상아,윤희림,이진영,전혜빈,박수이,Kim, Yu-jin,Kim, Sang-a,Yun, Hee-rim,Lee, Jin-young,Jeon, Hye-bin,Park, Su-e 한국정보통신학회 2019 한국정보통신학회논문지 Vol.23 No.1

최근 의료 기술의 발달로 제1형 당뇨에 대한 진단율이 높아지면서 당뇨 환아가 증가하고 있다. 이러한 상황에서 환아들에게 당뇨 교육이 필요하지만, 효율적이고 적절한 교육 콘텐츠는 부재한 상황이다. 특히 이러한 교육은 혈당 변화 대처요령과 행동치료에 대한 꾸준하고 반복적인 학습이 필요하다. 따라서 본 연구에서는 10-11세 환아들의 눈높이에 맞춰 주도적으로 학습할 수 있는 에듀테인먼트 콘텐츠를 제안하고자 한다. 효과적인 학습을 위해 주사 실습콘텐츠를 구성하였고 반복적인 학습을 위해 하이브리드방식을 사용하였다. 사용성 테스트를 통해서 이러한 구성이 효과적임을 알 수 있었다. 본 연구는 상대적으로 당뇨 교육을 받기 어려운 지역의 환아들이 쉽게 접근 가능함과 동시에 앞으로 개발될 아동의 눈높이에 맞춘 당뇨 교육 콘텐츠 연구의 기반 연구로써 공헌할 것으로 기대된다. With the recent development of medical technology, the diagnosis rate of type 1 diabetes is increasing and patients are increasing. However, diabetes education content is not aligned with the interest level of children. As a result of the interviews with experts, It was found that the measures of coping with the change of blood sugar and the behavior therapy require steady and repetitive learning. Therefore, this study proposes Edutainment content which can be repeatedly Learned by 10~11year old children. For effective learning, the contents of the laboratory practice were constructed and the hybrid method was used for the repetitive learning. Usability test showed that this configuration is effective. This study is expected to contribute to the study of diabetic education content that is suitable for the children's level of understanding which will be developed easily in the future.

부종요소 단백질의 정제 및 특성분석 연구

김유진,Kim, Yu-Gene 한국군사과학기술학회 2011 한국군사과학기술학회지 Vol.14 No.4

Edema factor(EF) is a portion of anthrax toxin which produces edema when combined with protective antigen. This paper describes about technique for cloning, expression, purification and activity test of EF. Using the E. coli expression system, we could make recombinant EF protein although it's origin is Bacillus anthracis. And also we could culture massively and purify highly pure protein. Finally we confirm a enzyme activity of purified EF to increase intracellular cAMP level. Through establishing this technique, it can be possible to research about EF in depth and apply to expression and purification of many other protein in biology.

Pin-site scar revision with subcisions and acellular dermal matrix

김유진,Ji Hun Kim,김윤상,전영우 대한미용성형외과학회 2020 Archives of Aesthetic Plastic Surgery Vol.26 No.1

External fixation is widely used in orthopedic and reconstructive surgery. However, it may leave retracted and depressed scars because it involves the percutaneous placement of transosseous pins or wires that are secured to an external scaffolding. These scars do not resolve or improve spontaneously, causing patients to experience dissatisfaction and possibly requiring a surgical intervention. Moreover, the management of scars that arise from external fixator pin sites is challenging because of their fibrotic and depressive features. However, by using subcisions and inserting acellular dermal matrix under the scars, the contour of the scars can be improved easily. Therefore, we present a case of pin-site scar revision using a technique that we developed.

Disinfection of various materials with 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride in hatchery facilities

김유진,Kim Jun-Beom,송창선,Nahm Sang-Soep 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.4

Objective: Surface disinfection is important in the proper running of livestock farms. However, disinfection of farm equipment and facilities is difficult because they are made of different materials, besides having large surface areas and complex structures. 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride (Si-QAC) is a quaternary ammonium salt-based disinfectant that attaches to various surfaces by forming covalent bonds and maintains its disinfecting capacity for a considerable time. Our aim was to evaluate the potential use of Si-QAC for disinfection of farm equipment and facilities.Methods: The short- and long-term antimicrobial and antiviral effects of Si-QAC were evaluated in both laboratory and farm settings using modified quantitative assessment method based on the standard operating procedures of the United States Environmental Protection Agency.Results: Si-QAC was highly effective in controlling the growth of the Newcastle disease virus and avian pathogenic Escherichia coli. Electron microscopy revealed that the mechanism underlying the disinfection activity of Si-QAC was associated with its ability to damage the outer membrane of the pathogen cells. In the field test, Si-QAC effectively reduced viral contamination of surfaces of equipment and space.Conclusion: Our results suggest that Si-QAC has great potential as an effective chemical for disinfecting farm equipment and facilities. This disinfectant could retain its disinfection ability longer than other commercial disinfectants and contribute to better farm biosecurity.