기관지 내시경으로 초기에 제거할 수 없었던 기도 이물 : 2례 보고

김연수,남승연,곽병곤,장우익,박경택,김창영,류지윤,Kim, Yeon-Soo,Nam, Seung-Yeon,Kwak, Byeong-Gon,Chang, Woo-Ik,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2

Foreign body aspiration is a cause of the accidental death at home. Therefore, early intervention and proper management is important. A bronchoscopy is indicated whenever there is a suggestive history and medical opinion. Occasionally, foreign body removal with bronchoscopy may be fail. But, on the situation, there is no definite recommended standard management. We experienced two cases of bronchial foreign body could not be removed with bronchoscopy at first intervention. The one was diagnosed too late. Endobronchial granulation tissue and edema made it impossible to find the foreign body at first bronchoscopy. After steroid and antibiotic therapy, foreign body could be removed with secondary bronchoscopy. Another was bronchial foreign body jammed tightly bronchus intermedius. Even after medical therapy, patient got aggravated. So foreign body was removed with bronchotomy.

소 유방염 유래 Staphylococcus aureus의 AFLP 지문분석

김연수,김상균,황의경,Kim, Yeon-soo,Kim, Sang-kyun,Hwang, Eui-kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2

Amplified fragment length polymorphism(AFLP) technique is based on the polymorphism detection through selective PCR amplification of restriction fragments from digested genomic DNA and thus includes the procedures of the total DNA digestion by endonucleases, ligation of adapters to the ends of the fragments, and following the selective amplification of the restricted DNA fragments. This study were aimed to : (1) determine the genetic variability of S aureus strains, (2) estimate genetic diversity within and among these strains, (3) compare phylogenetic relationships among these strains as genetic markers using AFLP techniques. Genomic DNA was digested with a particular combination of three restriction enzymes with specific recognition sites and the DNA fragments were ligated to restriction specific adapters and amplified using the selective primer combinations. In the S aureus strain, the number of scorable AFLP bands detected per each primer combination varied from 29 to 102, with an average of 61.59 using 27 primer combinations. A total of 1,663 markers were generated, 904 bands of which were polymorphic, showing a 33.48% level of polymorphism with these primer combinations. Among the primer combinations, E02/T02, E02/T03, E04/H02, E02/T01 and E04/H03 primer combinations showed a high level of polymorphism with 0.78, 0.76, 0.74, 0.71 and 0.70, respectively. But T03/H01, E01/T02 and E01/T03 primer combinations showed a low level of polymorphism with 0.38, 0.37 and 0.15, respectively, Therefore, the former primer combinations will be the most effective for AFLP analysis of S aureus. In SA1 sub-types the level of polymorphism of S aureus KCTC 1927 was similar to that of S aureus CU 01(0.825) and higher than those of other strains such as S aureus CU 02 (0.715), S aureus KCTC 2199(0.625), S aureus KCTC 1916(0.607) and S aureus KCTC 1621 (0.553). In SA2 sub-types the level of polymorphism of S aureus CU 07 was similar to that of S aureus CU 08(0.935) and higher than those of both S aureus CU 04(0.883) and S aureus CU 05(0.883) and lower than those of S aureus CU 03(0.583). In SA3 subtypes the level of polymorphism of S aureus CU 11 was similar to that of S aureus CU 12(0.913) and lower than that of S aureus CU 15(0.623). The results proved that AFLP marker analysis of S aureus strain could be used to study the epidemiology of mastitis and in addition, common genotype in geographic region could be useful for the development of an effective vaccine or DNA marker for easy diagnosis of mastitis caused by S aureus infection.

Hepatitis B Virus의 표면항원 유전자의 Cloning과 발현에 관하여

김연수,강현삼,Kim, Yeon-Soo,Kang, Hyen-Sam 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.1

사람의 피로부터 hepatitis B virus(HBV)를 순수 분리한 뒤 endogenous DNA polymerase 반응에 의해 완전한 double strand HBV DNA를 얻었다. 이 circular double stranded DNA를 BamHI으로 절단한 둬 pBR322에 삽입시켜 HBV의 total genome을 포함하는 재조합 plasmid pHBV-315를 제조하였다. cloning된 viral genome을 분석하기 위해 pHBV-315의 제한효소 지도를 작성하고 일부 염기 서열을 비교 조사하였다. 이 결과로부터 subtype adr HBsAg 유전자가 포함된 HBV DNA가 cloning 되었음을 알 수 있었다. pHBV-315로 부터 pHBV-15를 재조합하여 LMTK-세포와 E. coli에서 HBsAg 유전자의 발현을 조사하였다. 그 결과 LMTK-세포에서는 HBsAg이 검출되었고 E. coli에서는 검출되지 않았다. Dane particles were purified from human plasma and the DNA containing the single strand region was filled-in by endogenous DNA polymerase. Recombinant plasmid pHBV-315 was constructed by inserting the total HBV DNA into pBR322 DNA. Restriction enzyme map and nucleotide sequence of pHBV-315 suggested that the HBsAg subtype of the cloned HBV DNA is adr and how HBsAg gene is arranged in pHBV -315. pHBV -15 was constructed to direct the expression of HBsAg gene. When LMTK-cells and E.coli were transformed by pHBV-15, HBsAg was produced in LMTK-cells but not in E.coli.

Simian Virus 40 복제 원점 부위의 특정 염기서열의 기능

김연수,하정실,강현삼 ( Yeon Soo Kim,Jeong Sil Ha,Hyen Sam Kang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3

To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.

강원도 사육 한우의 간질 감염실태

김연수,김상균,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

A field survey of fascioliasis of Korean native cattle raising and raised in specialized commercial breeding farms and local farms in Kangwon province using both intradermal test and sedimentation technique for feces was carried out from November to December, 1996. Fecal samples were taken from fascioliasis positive cattle by the intradermal test for the fecal examination. Liver tissues were randomly collected from an abattoir for histopathological examination of liver fluke infection in cattle. The results are as follows. 1. By the intradermal test for a total of 211 cattle raising in both Wonju and Wheongsung, Kangwon province, 60 heads(28.4%) showed positive reaction. Among 60 positive cattle, eggs of Fasciola hepatica were found from 51 heads(85.0%) by sedimentation technique. 2. According to the cattle raising areas, the positive rates by the intradermal test were 26.7%(20 out of 75 heads) in Wonju and 29.4%(40 out of 136 heads) in Wheongsung. 3. According to the age of cattle examined, the positive rates by the intradermal test in 1~3, 4~6 and 7~10 years old were 11.7%(7 out of 100 heads), 68.3%(41 out of 93 heads) and 20.0%(12 out of 18 heads), respectively. 4. The overall infection rates of fluke larvae from the slaughtered cattle at an abattoir in Wonju was 24.7%(37 out of 150 heads). In histopathology, liver lesions were observed such as inflammation with infiltration of eosinophils, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissues, calcification and abscess formation.

DNA Amplified Fingerprinting 기법을 이용한 Salmonella pullorum과 Salmonella gallinarum의 다형성 비교 분석

김연수,김상균,송원철,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Song, Won-Chul,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

This study was performed to detect the Salmonella genus-specific DNA marker for comparing of polymophisms between S pullorum and S gallinarum by using PCR amplified techniques. A total of ten primers were used to detect DNA polymorphisms from S pullorum and S gallinarum. The number of DAF bands detected per each primer varied from 26 to 45, with an average of 32.7 using 10 primers. A total of 327 DAF bands were generated and among them 123 bands were polymorphic(37.6%). These DNA amplified fingerprinting(DAF) specific bands for S pullorum and S gallinarum were observed from all primers. For S pullorum, GEN 60-04, GEN 70-04 and GEN 70-03 primers showed a high level of polymorphism with 0.79, 0.70 and 0.57, respectively. But GEN 60-05 primer did not show a level of polymorphism. For S gallinarum, GEN 70-03, 60-04, 60-07, 70-05 and 70-04 primers showed a higher a low level of polymorphism from 0.16 to 0.28. Each five strains of S pullorum and S gallinarum were isolated from chickens showed typical clinical signs related with infection of pullorum disease or fowl typhoid at commercial chicken farms. DNA markers of these strains produced by GEN 70-04, GEN 70-05 and GEN 70-08 showed significant difference of band patterns between S pullorum and S gallinarum. These DNA markers could be used for comparison of DNA marker polymorphism between S pullorum and S gallinarum as well as rapid diagnosis of fowl typhoid and pullorum disease of domestic fowls.

Surgical Treatment of Mediastinal Schwannoma Showing Malignant Potentiality on Chest Magnetic Resonance Imaging Study : A Case Report

김연수,박경택,류지윤,김창영,장우익,장선희,Kim, Yeon-Soo,Park, Kyoung-Taek,Ryoo, Ji-Yoon,Kim, Chang-Young,Chang, Woo-Ik,Chang, Sun-Hee The Korean Bronchoesophagological Society 2008 大韓氣管食道科學會誌 Vol.14 No.2

Nerogenic tumor of various histologic types may arise in the posterior mediastinum. Mediastinal schwannoma is a frequent paraspinal neurogenic tumor, but malignant mediastinal schwannoma is rare tumor which is derived from Schwann cells. Although there are some reports dealing with approach for screening patients with symptoms suggesting malignancy and the imaging criteria for distinguishing malignant from benign schwannoma but the results are not clearly defined. We present a case of hugh mediastinal schwannoma which was taken for malignancy in imaging studies because of its invasiveness.

Functions of Early Palindrome Domain in SV40 DNA replication

김연수,하정실,강현삼,Kim, Yeon-Soo,Ha, Jeong-Sil,Kang, Hyen-Sam 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3

SV40 Replication core origin에 존재하는 early palindrome의 구조와 기능에 대해 알아보기 위해 SV40 origin을 포함하는 0.8kb DNA fragment를 filamentous phage M13 DNA에 cloning시킨 후 oligonucleotide-directed site-specific mutagenesis에 의해 early palindrome이 이룰 수 있는 hairpin 구조 중 stem 부분이 파괴된 mutant들과 base의 치환은 있으나 stem 부분은 다시 회복된 mutant들을 만들었다 in vivo replication assay를 위해 mutation이 일어난 origin 부위를 pAT153 vector로 subcloning하여 COS-1 cell에 transfection함으로써 replication level을 측정하였다. 그 결과 early domain의 inverted repeats는 fuctional cruciform structure를 형성함으로써 SV40 replication에 관여하는 것은 아니라는 것을 알 수 있었다. To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.

좌측 주기관지 근위부에서 발생한 무기폐를 동반한 횡문근 육종의 수술 치료 -1례 보고-

김연수,장우익,허진원,박시영,장선희,박경택,김창영,류지윤,조성준,Kim, Yeon-Soo,Chang, Woo-Ik,Huh, Jin-Won,Park, See-Young,Chang, Sun-Hee,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon,Cho, Seong-Joon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2

Treatment choice for primary pulmonary sarcoma is complete surgical resection. A 69 year old man developed dyspnea due to left lung atelectasis. There was endobronchial tumor completely obstructing the left main bronchus. The tumor was resected completely by main bronchial resection via a left thoracotomy incision, and diagnosed as leiomyosarcoma. Bronchoscopy and computed tomography in 6 months after operation, there was no evidence of recurrence.

완전삭제 도구 사용 흔적에 관한 연구

김연수,방제완,김진국,이상진,Kim, Yeon-Soo,Bang, Je-Wan,Kim, Jin-Kook,Lee, Sang-Jin 한국정보처리학회 2010 정보처리학회논문지 C : 정보통신,정보보안 Vol.17 No.2

The data wiping is a technique which perfectly deletes data in a storage to prevent data recovery. Currently, management of stored data is important because of increasing an accident of personal information leakage. Especially, if you need to discard data contained personal information, using a wiping tool which permanently deletes data to prevent unnecessary personal information leakage. The data wiping is also used for data security and privacy protection. However the data wiping can be used intentionally destruction of evidence. This intentionally destruction of evidence is important clues of forensic investigation. This paper demonstrates the methods for detecting the usage of wiping tools in digital forensic investigation. 완전삭제 기술은 저장장치 내 데이터를 복구가 불가능하도록 흔적 없이 완벽하게 삭제하는 기술이다. 최근 개인정보 유출 사고가 급증함에 따라 저장된 데이터의 관리가 중요해지고 있다. 특히 개인정보가 포함된 데이터를 폐기해야 하는 경우, 데이터를 영구적으로 삭제하는 완전삭제 도구를 사용함으로써 개인정보에 대한 불필요한 유출을 막을 수 있다. 또한 완전삭제 기술은 데이터 보안 및 프라이버시 보호 측면으로 활용 가능하다. 그러나 완전삭제 기술은 의도적으로 사건과 관련된 증거를 인멸하기 위해 사용될 수도 있다. 이러한 의도적인 증거 인멸은 사건 수사에 있어서 중요한 실마리가 될 수 있다. 본 논문에서는 디지털 포렌식 수사 과정에서 완전삭제 도구의 사용 흔적을 확인할 수 있는 방안을 제시한다.