MCP-1에 의해 유도된 THP-1 유주에 미치는 2-methoxy-1,4-naphthoquinone (MQ)의 영향

김시현,박보빈,홍성은,유성률,이장호,김사현,이평재,조은경,문철,Kim, Si Hyun,Park, Bo Bin,Hong, Sung Eun,Ryu, Sung Ryul,Lee, Jang Ho,Kim, Sa Hyun,Lee, Pyeongjae,Cho, Eun-Kyung,Moon, Cheol 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.2

본 연구는 2-methoxy-1,4-naphthoquinone (MQ)이 Monocyte chemoattractant protein-1 (MCP-1)에 의해 유도된 단구 유주에 미치는 효과를 알아보고자 진행되었다. 단구의 유주(migration) 현상은 신체 방어와 면역반응에 중요한 현상이다. MQ는 아시아권 국가에서 다양한 질병과 통증 치료에 민간요법으로 오랫동안 사용되었던 봉선화(Impatiens balsamina) 잎으로부터 추출한 주요 성분이다. 단구 세포주 THP-1를 이용하여 실험을 진행하였으며, MQ의 세포독성, MCP-1에 의해 유도된 유주 현상에 미치는 영향을 transwell system을 이용하여 확인하였다. MQ 작용기전을 이해하기 위해 cAMP 발현 및 Erk1/2 인산화를 각각 ELISA, Western-blot 기법을 통해 분석하였다. MQ의 단구 세포주 THP-1에 대한 MQ의 세포독성은 $10{\mu}M$ 농도에서 나타나기 시작했으며, $100{\mu}M$ 농도에서 약 50% 가량의 세포독성을 확인했다. 염증성 화학주성 인자 MCP-1에 의해 유도된 단핵구 세포주 THP-1의 유주현상은 MQ 처리 후 농도증가에 비례하여 증가하였으며, $0.1{\mu}M$ 농도의 MQ를 처리했을 때 가장 높은 증가를 보였다. MCP-1 단독 처리 시 감소하는 cAMP 의 배양액 내 발현은 MQ 동시 처리 시 더욱 감소하였고, 유주현상과 마찬가지로 $0.1{\mu}M$ 농도에서 가장 감소하였다. MCP-1의 수용체인 C-C motif chemokine receptor 2 (CCR2) 신호전달 과정에 관여하는 주요 신호전달 단백질인 Erk1/2의 인산화도 $0.1{\mu}M$ MQ 동시 처리 시 증가하였다. 이상의 결과를 통해 MQ가 MCP-1에 의해 유도되는 THP-1 세포주의 유주현상을 증가시키며, 연관된 cAMP 발현을 감소, Erk1/2 인산화는 증가시키는 경향을 확인하였다. This study examined the effects of 2-methoxy-1,4-naphthoquinone (MQ) on the monocyte chemoattractant protein-1 (MCP-1)-induced migration of monocytes, which is an important phenomenon for the body defense and immune response. MQ is a major component extracted from Impatiens balsamina leaves, which have been used for many years in Asian medicine for the treatment of a range of diseases and pain. The cytotoxicity of MQ began to appear at a concentration of $10{\mu}M$, and approximately 50% cytotoxicity was confirmed at $100{\mu}M$. The MCP-1 induced migration of the THP-1 monocyte cell line increased after MQ treatment in a dose dependent manner and the largest increase was observed at $0.1{\mu}M$. The level of cAMP expression decreased after a treatment with $0.1{\mu}M$ MQ. The phosphorylation of extracellular signal-regulated kinases 1/2 (Erk1/2), a key signaling protein involved in the signaling pathway of C-C motif chemokine receptor 2 (CCR2), a receptor for MCP-1, was increased by the simultaneous treatment of $0.1{\mu}M$ MQ. These results show that MQ increases the MCP-1-induced migration of THP-1, decreases the level of cAMP expression, and increases the level of Erk1/2 phosphorylation.

White Oganic Light-Emitting Diodes based on Simply Modified Anthracene and Rubrene

김시현,이승희,Kim, Si-Hyun,Lee, Seung-Hee The Korean Society of Applied Science and Technolo 2022 한국응용과학기술학회지 Vol.39 No.5

The white OLED is fabricated with the anthracene-based blue emitting material, 9-(2-naphthyl)-10-(p-tolyl)anthracene (2-NTA) in various volume-ratios of orange dopant, rubrene, which results in pure white emission with C.I.E. coordinate of ~(0.32, 0.39). The devices with <1.5% rubrene show better EL properties (efficiency) than >3% devices. Furthermore the turn-on voltage of 2-NTA WOLED (3.7 V) is lower than that of 2-NTA blue OLED (5.4 V) at the same condition. Conclusively 2-NTA with rubrene less than 1.5% (v/v) could be utilized for the pure WOLED.

학령기 아동의 문식성 중재를 위한 초등학교 1-2학년 국어 교과서 구문 분석

김시현(Si Hyun Kim),김화수(Wha soo Kim),김민성(Min Sung Kim) 한국언어치료학회 2016 한국언어치료학회 학술발표대회 논문집 Vol.2016 No.12

Purpose: Syntactic ability is one of the essential elements of literacy development of school age children. This study aims to analyze the Korean textbooks of elementary 1-2 grade in 2015 Revised National Curriculum to provide basic data regarding syntactic characteristics for literacy mediation of school age children. Methods: Sentences in Korean textbooks of elementary 1-2 grade were categorized by sentence ending. Then, it was categorized by simple and complex sentence by the relationship between subject and predicate, and then the subcategory of complex sentence was analyzed in-depth. Results: As the result of analyzing 2,272 sentences, the most common type of sentences in accordance with sentence ending was declarative sentence(54.05%), followed by propositive sentence(25.31%), interrogative sentence(13.07%), imperative sentence(5.33%), and exclamatory sentence(2.24%). Also, as for the classification of relationship between subject and predicate, frequency of complex sentences(69.06%) was higher than simple sentences(30.94%). In complex sentences, embedded sentence(67.40%) was more frequent than conjoined sentence(58.46%). As for the embedded sentence, adnominal clause(58.46%) was the most frequent sentence type followed by adverbial clause(23.01%), noun clause(15.10%), quotative clause(2.85%), predicate clause(0.58%). Also, as for the conjoined sentence, the subordinate conjoined sentence(50.47%) and coordinate conjoined sentence(49.53%) showed similar proportion. Conclusions: Based on the result, the study suggested including sentences frequently shown in textbooks to mediation goal in order to support reading comprehension and writing of school age children. The study is expected to be utilized as reference in mediation process of language evaluation of normal school age children and children with language learning disability.

보건복지부 사업을 중심으로 한언어재활사의 바우처 서비스 역량에 대한 연구

김시현(Si Hyun Kim),김화수(Wha Soo Kim) 한국언어치료학회 2017 言語治療硏究 Vol.26 No.1

Purpose: This study investigated the voucher service competency of speech-language pathologists and suggested methods for improving service competency by reconsidering the importance of speech-language pathologists in social services.Methods: This study conducted questionnaire surveys on 119 speech-language pathologists across Korea, and analyzed at total of 74 items, including seven items on the general characteristics of speech-language pathologists, 20 items on voucher service knowledge, 31 items on skills, and 16 items on attitude. Results: First, it was identified that knowledge in voucher service of speech-language pathologists was relatively low compared to the knowledge demand as they were reported to be on average 2.62 (SD=0.68) points and 3.58 points (SD=0.89) out of 5. Second, the level of skills as service personnel was 3.50 points (SD=0.94) on average, and in the order of developmental rehabilitation service, community social service investment project, and language development support services. Third, satisfaction and role related stress for voucher service were 3.83 points (SD=0.66) and 3.25 points (SD=0.81) respectively, and showed very positive attitude for the necessity of voucher but had high stress for document record. Fourth, there was a significant difference between age, final education, workplace, position, clinical experience, and certification steps for characteristics of subjects, and especially, clinical career showed a difference with knowledge, skill, satisfaction, and stress of the service.Fifth, knowledge of voucher service, skills, and attitude had a strong positive correlation. Conclusions: By reviewing these results comprehensively, this study suggested multidimensional cooperation methods to improve Korea s institutions, educational efforts for associations and schools, and individual training for improving voucher service competencies of speech-language pathologists.

한-EU 해상운송보안 제도 대응전략 비교연구

김시현(Si Hyun KIM),신건훈(Gun Hoon SHIN) 한국무역상무학회 2015 貿易商務硏究 Vol.68 No.-

According to the increasing attention to safety and security in maritime shipping, there are diverse security systems in international logistics activities. Although prior studies on maritime transport and port security reviewed security policy and practices in order to provide useful insights for strategic agenda, a few focused on attitude to respond it. Moreover, there are no prior study on a comparative study between continents. To tackle this, this paper compared confrontation attitude to maritime transport and port security between South Korea and European Union. Results identified that maritime shipping security incorporates container cargo security management, logistics security management, logistics security certificate system, and environmental impacts management caused by maritime shipping. Further, the comparison between two countries suggests that South Korea need to take more positive attitude to respond, such as investment in equipment and technologies for maritime shipping security, construction of comprehensive management system, political supports for logistics security, and training and education for safety and security. The results provide useful insights for strategic review of security systems in South Korea, and to help strategic agenda for future improvement.

현대 유기적 건축의 토포스 지향적 공간시퀀스 구조에 관한 연구

김시현(Kim, Si-Hyun),공순구(Kong, Soon-Ku) 대한건축학회 2016 대한건축학회 학술발표대회 논문집 Vol.36 No.2

We human beings all occupy space, and that space becomes the center of our life events. Architectural space thus acquires significance. In architecture, it is thought that the zeitgeist of a given era and placeness are deeply related to what is called "concepts of space". Therefore, it is important that we conduct an analysis of the past and future of a given place and how that place relates to the surrounding environment so that we can have a better understanding of architectural space and actually undertake construction.

고층 아파트 개구부의 비구조 RC 외벽체와 연결보 균열 제어를 위한 실험적 연구

김시현(Kim, Si-Hyun),김성현(Kim, Sung-Hyun),강수민(Kang, Su-Min),최경규(Choi, Kyoung-Kyu) 한국콘크리트학회 2023 한국콘크리트학회 학술대회 논문집 Vol.35 No.1

단순언어장애 및 비특정 언어장애 아동의 단어유추 추론능력

김시현(Si Hyun Kim),김화수(Wha Soo Kim) 한국언어치료학회 2022 言語治療硏究 Vol.31 No.4

유스케이스 모델을 활용한 마이크로서비스 기능 식별법

김시현(Si-Hyun Kim),오재원(Jaewon Oh) 한국정보통신학회 2023 한국정보통신학회논문지 Vol.27 No.7

전통적인 방식의 소프트웨어 아키텍처인 모노리식 아키텍처는 여러 서비스가 하나의 코드베이스에 담겨 있어 유지 보수가 어렵고 복잡하다. 반면에 비교적 최근 등장한 마이크로서비스 아키텍처의 경우 서비스들이 느슨하게 결합되어 있고, 각 서비스를 독립적으로 개발하고 배포할 수 있어 유지 보수가 쉽고 확장성이 뛰어나다. 이에 따라 모노리식 아키텍처를 마이크로서비스 아키텍처로 전환하기 위해 기존 모노리식 웹 앱으로부터 마이크로서비스를 식별하는 다양한 연구들이 제안되고 있다. 그러나 기존 연구들은 식별 결과로 각 마이크로서비스가 어떤 구성 요소를 갖는지에 관한 정보를 제공할 뿐, 각 마이크로서비스가 어떤 기능을 수행하는지에 관한 정보는 제공하지 않는다. 본 논문은 식별된 마이크로서비스의 기능을 이해하기 위한 새로운 접근 방식을 제안한다. 이 접근 방식에서는 유스케이스 모델을 사용하여 모노리식 웹 앱의 기능을 표현하고, 이 웹 앱으로부터 식별된 마이크로서비스의 기능을 마이크로서비스에 속한 컴포넌트들에서 추출된 핵심 단어들로 표현한다. 최종적으로 유스케이스와 마이크로서비스의 핵심 단어들을 대응시킴으로써 마이크로서비스가 제공하는 기능을 식별한다. 그리고 오픈 소스 웹 앱을 대상으로 실험을 진행하여 제안하는 기법의 유용성을 검증한다. A monolithic architecture contains all services in a single codebase, whereas each service is loosely coupled with other services and can be deployed independently in a microservice architecture. Therefore, multiple studies have been proposed to identify microservices from monolithic web applications. However, these existing approaches only provide structural information about identified microservices, such as the components each of the microservices contains, but do not provide semantic information about what functionality each microservice performs. This paper proposes a novel approach to understanding the functionalities of the identified microservices. This approach uses a use-case model to represent the functionalities of the monolithic application. The functionality of each identified microservice is then modeled as keywords extracted from the components consisting of the microservice. Finally, the functionalities of the microservices are identified by mapping between the use-cases and the keywords of the microservices. Experiments are conducted on open-source web applications to validate the effectiveness of the proposed approach.

초등학교 1-2학년 국어 교과서 구문 분석

김시현(Si Hyun Kim),김민성(Min Sung Kim),유영주(Young Ju Yoo),김화수(Wha Soo Kim) 한국언어치료학회 2018 言語治療硏究 Vol.27 No.3