한약재 추출물이 마우스 조골세포의 골분화에 미치는 영향

유성률 한국보건기초의학회 2016 보건기초의학회지 Vol.9 No.1

홍화, 홍화씨 추출물이 MC3T3E1 세포의 골분화 과정에 미치는 영향

유성률,신선미,Yu, Sung-ryul,Shin, Seon-mi 대한한방내과학회 2015 大韓韓方內科學會誌 Vol.36 No.4

Objectives This study investigated the effect of purified safflower (Carthamus tinctorius Linne) and safflower seed (Carthamus tinctorius L. seed; CS) extract, using hot water and ethanol extract methods , on the osteogenic differentiation of MC3T3E1 cells.Methods The safflower and safflower seed were extracted with hot water and ethanol. The samples were concentrated by a rotary evaporator and then freeze-dried using a freeze-dryer. The MC3T3E1 cells were propagated and maintained in DMEM (Gibco) containing 10% FBS and a 1% antibiotic antimycotic solution. To induce osteogenic differentiation, the cells were treated for 14 days with DMEM with 10 mM β-glycerophosphate and 50 μM ascorbic acid. Extract doses were confirmed by the results of an MTT assay, and treatment of the extracts was performed in a differentiation medium every two days. The ALP staining and activity were tested after osteogenic differentiation for five days, and after 14 days, osteogenic differentiation was determined by alizarin red S staining. The mRNA expressions of osteogenic-related genes were quantified using quantitative real-time PCR.Results In the results of the MTT assay, all concentrations of safflower extracts had no toxicity in the MC3T3El cells. But in the groups of 100 ng/ml and 200 ng/ml concentrations of safflower seed extracts, the cell viability was significantly reduced by up to 40-50%. So we fixed the treatment concentration of the extract at 50 ng/ml. In the ALP and alizarin red S staining, all extract groups increased osteogenic differentiation compared with the control group. The water-safflower extract group showed the highest mRNA level of Alp, Runx2, and Dlx5 genes. The mRNA level of Ocn, an osteogenic gene related to late-stage differentiation, in the ethanol-safflower extract group increased the mineralization more significantly than in other groups.Conclusions These data suggest that the extract of safflower increases the osteoblastic differentiation activates of MC3T3E1 cells like the extract of safflower seed. The water-extract and ethanol-extract of safflower have effects on different stages of osteogenesis in MC3T3El. Not only safflower seed but also safflower will be useful therapeutic reagents for age-associated chronic diseases such as osteoporosis.

홍화 추출물이 생쥐 골수 유래 중간엽 줄기세포의 지방분화에 미치는 영향

유성률,신선미,Yu, Sung-ryul,Shin, Seon-mi 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.1

Objective: This study investigated the effect of purified Carthamus tinctorius (C. tinctorius) extracted with a hot water and ethanol method on adipogenic differentiation of mouse bone marrow-derived mesenchymal stromal stem cells (mBMSCs). Methods: The C. tinctorius was extracted using hot water and ethanol. The samples were concentrated by a rotary evaporator and were then dried using a freeze-dryer. The mBMSCs were cultured and maintained in a minimum essential medium eagle alpha (${\alpha}-MEM$) supplemented with 10% FBS and 1% antibiotic antimycotic solution. To induce adipogenic differentiation, the cells were treated with Dulbecco's modified eagle's medium-low glucose (DMEM-LG) containing 1 mg/mL insulin, 1 mM dexamethasone, and 0.5 mM 3-isobutyl-1-methylxanthine. To evaluate the adipogenic differentiation ability, oil-red O staining was performed after adipogenic differentiation for 21 days. The mRNA expression and protein level of adipogenic-related genes were quantified by quantitative real-time PCR and western blotting, respectively. Results: In the results of the MTT assay, no concentrations of C. tinctorius extracts showed toxicity on mBMSCs, so we fixed the treatment concentration of the extract at 100 ng/mL. In oil-red O staining, the water-C. tinctorius extract treatment significantly decreased adipogenic differentiation compared with the control and ethanol extract groups. The water-C. tinctorius extract group in particular showed reduced mRNA and protein expression of Peroxisome proliferator-activated receptor gamma ($Ppar{\gamma}$) and CCAAT/enhancer-binding protein alpha ($C/ebp{\alpha}$), which are adipogenic-related transcription factors. Conclusion: These data suggest that extract of C. tinctorius decreased the adipogenic differentiation of mBMSCs, while only water-C. tinctorius extract had an effect on different adipogenesis in mBMSCs. The C. tinctorius will be a useful therapeutic reagent for the prevention of obesity-related diseases such as diabetes, hyperlipidemia, coronary artery disease, and osteoporosis.

택사/저령 추출물이 사람 중간엽 줄기세포의 지방세포 분화에 미치는 영향

유성률,김시현,신선미 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.6

Objective: This study investigated the effect of purified medical herb extracts such as Alisma canaliculatum and Polyporus umbellatus on adipogenic differentiation of human bone marrow derived mesenchymal stromal stem cells (hBMSCs). Methods: Two different medical herb were extracted using hot distilled water. The optimal concentration of extracts were fixed at 100 ng/ml by means of cell viability and cytotoxic assay. To test the adipogenic differentiation ability of extracts, we induced the adipogenesis of hBMSCs for 21 days. At day 5, the cell was harvested to check mRNA and protein expression level of adipogenic related factors. The efficacy of lipid droplet formation was evaluated using the oil-red O staining method at days 21. Results: Two different medical herb extracts have no toxicity on hBMSCs. And two different medical herb extracts significantly decreased formation of lipid droplet compared with control groups in hBMSCs. The A. canaliculatum extract group showed the lowest mRNA and protein expression level of adipossgenic related transcription factors. This data suggests that extract of A. canaliculatum and P. umbellata decrease the adipogenic differentiation of hBMSCs. Conclusions: Our findings indicate that water-extract of A. canaliculatum and P. umbellata will be useful therapeutic reagents for prevention of obesity related disease such as diabetes, hyperlipidemia, coronary artery disease, and osteoporosis.

대학생들의 건강관련 삶의 질 관련요인

유성률(Sung Yul Yu),최희영(Hee Young Choi) 한국보건기초의학회 2023 한국보건기초의학회지 Vol.16 No.1

성장호르몬이 단구 세포주 THP-1과 U937의 활성에 미치는 영향 평가

문철,유성률,김시현,성현경 한국보건기초의학회 2022 한국보건기초의학회지 Vol.15 No.2

Growth hormone (GH) is a peptide hormone secreted by the pituitary gland and stimulates cell proliferation and division to promote tissue growth. In addition, the development of the immune system and the regulation of the immune response have been reported. Along with the research results related to the inflammatory control function, a correlation between the severe symptoms of COVID-19 in the elderly and a decrease in the secretion of growth hormone has recently been suggested. Therefore, this research team studied the effect of growth hormone on the proliferation and inflammatory cytokine secretion of monocyte cell lines THP-1 and U937. As a result, it was confirmed that the proliferation of THP-1 and the secretion of TNF-α and IL-1β induced by LPS were decreased by growth hormone. On the other hand, the proliferation of U937 cell line was increased by growth hormone treatment, whereas the secretion of TNF-α and IL-1β was hardly affected. In the future, the analysis of the GH receptors expressed by the two cell lines and the study of the molecular mechanism of GH action are required. In addition, it is considered that the cell stimulation technique using Poly(I:C) needs to be established more accurately.

한국의 임상검체로부터 분리된 해외유입 두 형태 진균에 대한 연구

이장호,유성률,구본경,문철 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.1

Dimorphic fungi are characterized by the production of mycelia in the environment or when incubated at lower temperatures (25∼30°C) on media. The conversion to yeast occurs when invading a human body or when incubated at elevated temperatures (35∼37°C). Dimorphic fungi cause systemic infection and occur only in endemic regions; they can cause deep mycosis, which invades tissues and organs. Imported thermally dimorphic fungal pathogen has five genera, such as Coccidioides immitis, Histoplasma capsulatum, Blastomyces dermatitidis, Talaromyces (Penicillium) marneffei, and Paracoccidioides brasiliensis. Thus far, 30 cases of dimorphic fungi have been reported in Korea, including C. immitis in 18, H. capsulatum in 5, B. dermatitidis in 3, and T. marneffei in 4 cases. Three 3 cases of C. immitis, 2 cases of H. capsulatum, 1 case of B.dermatitidis, and 1 case of Talaromyces (Penicillium) marneffei were reported in Korea, even though no experience of an epidemic was present. None the dimorphic fungi except for Sporothrix schenkii (Dimorphic fungi) occur in Korea but are caused by endemic fungi that can be found only in specific regions and limited areas of foreign countries. Considering the trends of migratory locals and the increasing number of immigrants living in and returning home, the incidence of imported infections is expected to increase. 두 형태 진균은 자연환경이나 배지에 접종하여 실온에(25∼ 30°C)에서 배양하면 균사체를 형성하는 특성을 가지고 있으며, 체온이나 높은 온도(35∼37°C)에서 배양을 하면 효모형태를 나타나는 특성이 있다. 풍토병 지역에서만 발생하는 이들은 전신 감염을 일으키며, 조직이나 기관에 침입하여 심부 진균 증을 일으키는 원인 진균이다. 사람에 감염되는 두 형태 진균은 지금까지 Blastomyces dermatitidis, Coccidioides immitis, Sporothrix schenkii, Talaromyces (Penicillium) marneffei, Histoplasma capsulatum, 그리고, Paracoccidioides brasiliensis 여섯 균종으로 알려져 있다. 지금까지 해외에서 유입되어 국내에 보고된 두 형태 진균은 Coccidioides immitis가 18예, Histoplasma capsulatum이 5예, Blastomyces dermatitidis가 3예, 그리고 Talaromyces (Penicillium) marneffei 가 4예로 모두 30예의두 형태 진균이 보고되었다. 유행지역의 방문경험이 없음에도 국내에 발생된 경우는 C. immitis가 3예, H. capsulatum이 2 예, B. dermatitidis가 1예, 그리고 Talaromyces (Penicillium) marneffei 가 1예로 있다. Sporothrix schenkii 를 제외한 모든 두 형태 진균이 국내에서는 발생하지 않고 외국의 특정 국가와 한정된 지역에서만 발생하는 풍토병 원인의 토착 진균이지만, 유행 지역의 여행 경험이나 호발 지역의 이민 거주자들의 귀국과 국내로 유입되는 외국인의 증가 추세를 고려하면 해외유입 감염증의 발생은 점점 증가할 것으로 사료된다.

양성발작성두위어지럼증을 치료한 후 반고리관내 이석유입 변환에 의한 치료 증가 사례 연구

김철승,유성률 한국보건기초의학회 2020 한국보건기초의학회지 Vol.13 No.1

The purpose of this study was to investigate cases in which the number of treatments for benign paroxysmal positional vertigo increased due to changes in the semicircular canal inflow of the otolith. Video Nystagmus testing was conducted on 1,450 patients who visited Suncheon S Hospital in Jeollanam-do, South Korea. 496 patients were treated from May 2018 to December 2019. A survey was conducted on 31 patients whose semicircular canal inflow area was abnormal. The number of these patients that had three or more canalith repositioning maneuver treatments was 42.8%, which is much higher than the 8.5% for other patients with benign paroxysmal positional vertigo. The prognosis factors that increase the number of treatments requiring the canalith repositioning maneuver include idiopathic conditions, cerebral infarction, trauma, migraine, tinnitus, vestibulopathy, cerebral infarction, and cerebrovascular diseases. Therefore, patients who have had an increased number of treatments of the canalith repositioning maneuver should have a follow-up examination for the prognosis factors that can frequently cause otolithiasis. Even if dizziness improves after the canalith repositioning maneuver it is still prudent to do simultaneous prognosis factor treatment.

임상병리(학)과 실험 실습기자재 표준화에 대한 설문조사 분석

신경아,유성률,김철승 한국보건기초의학회 2019 보건기초의학회지 Vol.12 No.2

The purpose of this study was to create a standardized list of equipment necessary to set up a biomedical laboratory science department. A survey of professors that worked in university biomedical laboratory science departments was conducted between May 10, 2019 and August 31, 2019. Their responses were then applied to the Likert scale. The equipment with a score of 4.5 or higher was identified as equipment that was highly necessity for a biomedical laboratory science department. In this study, we seperated the necessary equipment by clinical and urinary chemistry, hematology, transfusion, microbiology, clinical physiology, histopathology and cytology, immunoserology and molecular biology laboratories and common materials. This study is expected to be used as data for the standardization of equipment in biomedical laboratory science departments, which is an important step towards the standardization of curriculum of said departments.

MCP-1에 의해 유도된 THP-1 유주에 미치는 Zerumbone의 영향

김사현,김시현,유성률,이평재,문철,Kim, Sa Hyun,Kim, Si Hyun,Ryu, Sung Ryul,Lee, Pyeongjae,Moon, Cheol 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.2

본 연구는 zerumbone이 단구의 유주에 어떠한 영향을 미치는지 알아보고자 진행되었다. 단구는 다양한 염증 질환의 중요한 매개자로 인식되고 있으며, 활성, 유주 등 단구의 기능 조절을 통해 염증 질환을 조절하는 가능성이 보고 되고 있다. 염증 발생 시 증가하는 케모카인인 MCP-1에 의해 단핵구 세포주 THP-1의 유주가 유발되는 것을 확인하였다. 10 ng/mL의 농도에서 유주가 발생하였으며, 100 ng/mL과 200 ng/mL의 농도에서 가장 높은 유주 현상이 나타났다. MCP-1에 의해 유발된 THP-1 유주는 zerumbone 존재 시 50% 이상 감소하였다. MCP-1 수용체인 CCR2 신호전달 과정의 중요 2차 전달자인 cAMP의 배양액 내 농도는 zerumbone 단독 처리 시 세포 단독 배양 조건에 비해 증가하였으며, MCP-1 단독 처리 시에는 의미있게 감소하였다. 그러나, zerumbone과 MCP-1을 동시에 처리했을 때에는 다시 cAMP의 증가가 관찰되었다. MCP-1 처리에 의해 일어나는 Erk 인산화도 zerumbone과 동시 처리 시 감소하는 결과를 확인했다. 본 연구는 염증성 질환에 중요한 매개자로 인식되고 있는 단구의 유주 현상을 조절하는 zerumbone의 가능성을 보여준다. This study examined the effects of zerumbone on monocyte migration. Monocytes are recognized as important mediators of various inflammatory diseases, and the possibility of controlling inflammatory diseases by regulating the monocyte functions, such as activity and mobility, has been reported. MCP-1, which is a chemokine with levels that increase upon inflammation, causes the migration of the monocyte cell line, THP-1. Migration occurred at a concentration of 10 ng/mL MCP-1, and the highest migration occurred at 100 ng/mL and 200 ng/mL. MCP-1-induced THP-1 migration decreased by more than 40% in the presence of zerumbone. The concentration of cAMP, an important secondary messenger of the CCR2 signaling pathway, the MCP-1 receptor, was increased in the culture medium after a zerumbone treatment. The concentrations of cAMP decreased significantly under the MCP-1 treatment condition only. On the other hand, an increase in cAMP was observed when zerumbone and MCP-1 were treated simultaneously. Erk phosphorylation induced by an MCP-1 treatment was also found to decrease with the zerumbone treatment. This study introduces the possibility of controlling inflammatory diseases through the function of zerumbone, which regulates the migration of monocytes.