소 β-casein 유전자 영역에서 소 Insulin-like Growth Factor 1을 생산하기 위한 Knock-in Vector

김상영,박다솜,김세은,구덕본,강만종,Kim, Sang Young,Park, Da Som,Kim, Se Eun,Koo, Deog-Bon,Kang, Man-Jong 한국동물번식학회 2017 Reproductive & developmental biology Vol.41 No.3

The production of therapeutic protein from transgenic domestic animal is the major technology of biotechnology. Insulin-like growth factor-1 (IGF-1) is known to play an important role in the growth of the animal. The objective of this study is construction of knock-in vector that bovine IGF-1 gene is inserted into the exon 7 locus of ${\beta}$-casein gene and expressed using the gene regulatory DNA sequence of bovine ${\beta}$-casein gene. The knock-in vector consists of 5' arm region (1.02 kb), bIGF-1 cDNA, CMV-EGFP, and 3' arm region (1.81 kb). To express bIGF-1 gene as transgene, the F2A sequence was fused to the 5' terminal of bIGF-1 gene and inserted into exon 7 of the ${\beta}$-casein gene. As a result, the knock-in vector is confirmed that the amino acids are synthesized without termination from the ${\beta}$-casein exon 7 region to the bIGF-1 gene by DNA sequence. These knock-in vectors may help to create transgenic dairy cattle expressing bovine bIGF-1 protein in the mammary gland via the expression system of the bovine ${\beta}$-casein gene.

감정자유기법(EFT)의 연구를 중심으로 본 경락기반심리요법의 동향

김상영,인창식,최인원,김종우,Kim, Sang Young,Yin, Chang Shik,Choi, In Won,Kim, Jong-Woo 대한한방신경정신과학회 2013 동의신경정신과학회지 Vol.24 No.suppl1

Objectives : The purpose of this study is to investigate the researches about regarding Emotional Freedom Technique (EFT) and to understand the trends of Meridian-based Psychotherapy. Methods : Every article relevant to EFT was obtained from 'Pubmed' and Korean journal databases. Keywords used for searching included 'EFT' and 'Emotional freedom technique'. Results : 1) 5 reviews, 11 randomized controlled trials, 3 controlled trials, 1 single group comparative study and 4 case studies were searched. 2) Anxiety disorders were most frequently studied with EFT. Other studies included insomnia, depression and pain symptoms. EFT interventions were attempted in many different protocols and assessed with various tools. 3) Review articles indicated that meridian-based psychotherapies, such as EFT, are based on the meridian theory of oriental medicine. They evaluate EFT positively for its effectiveness on psychiatric diseases. Conclusions : EFT is increasingly studied and used in clinical practice of various fields. Objective evaluation tools and standardized intervention protocols are needed for the development of a new guideline for EFT.

양성자 자기공명분광법을 이용한 우울증 동물모델에서의 항우울제 약물 효능 평가

김상영,최치봉,이성호,우동철,윤성익,홍관수,이현승,정재준,지보근,홍승탁,김휘율,최보영,Kim, Sang-Young,Choi, Chi-Bong,Lee, Sung-Ho,Woo, Dong-Cheol,Yoon, Seong-Ik,Hong, Kwan-Soo,Lee, Hyun-Sung,Cheong, Chae-Joon,Jee, Bo-Keun,Hong, Sung-Tak,K 한국의학물리학회 2008 의학물리 Vol.19 No.2

본 기초연구에서는 강제수영검사(forced swimming test, FST)에 의해 우울증이 유발된 쥐의 해마(hippocampus) 부위에서 뇌 대사물질(brain metabolites)중에 choline 신호의 변화를 관찰하였다. 본 연구의 목적은 우울증이 유발된 쥐에 항우울제인 desipramine-HCl을 투여하여 정상 쥐와 약물을 투여한 쥐 사이의 뇌 대사물질의 차이를 자기공명분광법(magnetic resonance spectroscopy: MRS)을 통하여 알아보는 것이다. 실험대상으로는 6주령의 SD rat을 사용하였으며 우울증을 유발시키고 항우울제의 약물평가를 위한 모델링 방법으로 강제수영검사를 시행하였다. 자기공명분광법을 시행하기 위한 localization 방법으로는 PRESS 펄스시퀀스를 사용하였다(TR: 2,500 ms, TE: 144 ms, averaging: 512, complex data point: 2048, scan time: 25 min). 정상 쥐 그룹과 우울증을 유발시킨 뒤 항우울제를 투여한 쥐 그룹 모두 양쪽 해마부위에서의 NAA/Cr, Cho/Cr 비율(ratio)은 차이가 나지 않았다. 또한 왼쪽 해마에서 정상 쥐와 항우울제를 투여한 쥐 사이에서 NAA/Cr, Cho/Cr 비율도 차이가 나지 않았고, 오른쪽 해마부위에서도 통계적으로 유의한 차이가 없었다. 본 연구를 통하여 anti-immobility효과를 나타내는 항우울제를 투여함으로써 choline 신호가 정상 쥐와 비슷하게 회복되는 것을 관찰하였다. 이는 앞으로의 항우울제 약물 평가에 있어 자기공명분광법을 도입함으로써 기존의 주관적인 판단을 배제할 수 없었던 행동변화분석만으로 항우울제의 효과를 평가하는 방법에 비해 객관적이고 정량적인 분석을 할 수 있다는 점에서 아주 유용한 방법이라 사료된다. In this study, we observed the alteration of choline signal intensity in hippocampus region of the depressive rat model induced by forced swimming test (FST). The purpose of this study was to evaluate the antidepressant efficacy in the depressive animal model using MR spectroscopy. Fourteen experimentally naive male Sprague-Dawley rats weighting $160{\sim}180\;g$ were used as subjects. Drug injection group was exposed to the FST except for control group. The drugs were administered subcutaneously (SC) in a volume equivalent to 2ml/kg. And three injections were administered 23, 5, and 1h before beginning the given test. 1H MR spectra were obtained with use of a point resolved spectroscopy (PRESS) localization sequence performed according to the following parameters: repetition time, 2500 ms; echo time, 144 ms; 512 average; 2048 complex data points; voxel dimensions, $1.5{\times}2.5{\times}2.5\;mm^3$ ; acquisition time, 25min. There were no differences in NAA/Cr and Cho/Cr ratio between the right and the left hippocampus both normal control rats and antidepressant-injected rats. Also, no differences were observed in NAA/Cr and Cho/Cr ratio between the normal control rats and the antidepressant-injected rats both the right and the left hippocampus. In this study, we found the recovery of choline signals in the depressive animal model similar to normal control groups as injecting desipramine-HCl which was antidepressant causing anti-immobility effects. Thus, we demonstrated that MR spectroscopy was able to aid in evaluating the antidepressant effect of desipramine-HCl.

다중 홉 시스템에서 성능 향상을 위한 중계기 선택 기법

김상영(Sang-Young Kim),하창빈(Chang-Bin Ha),송형규(Hyoung-Kyu Song) 한국통신학회 2017 한국통신학회 학술대회논문집 Vol.2017 No.6

치은연하치태내의 Trypsin - like Activity 와 치은열구액삼출량과의 관계에 대한 임상적 연구

김상영(Sang Young Kim),김병옥(Byung Ok Kim),한경윤(Kyung Yoon Han) 조선대학교 구강생물학연구소 1992 Oral Biology Research (Oral Biol Res) Vol.16 No.2

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셀룰러 시스템에서 향상된 성능을 위한 협력 프리코딩 기법

김상영(Sang-Young Kim),김용준(Yong-Jun Kim),송형규(Hyoung-Kyu Song) 한국통신학회 2016 한국통신학회 학술대회논문집 Vol.2016 No.1

폴리옥시에틸렌 메타크릴레이트 반응성 계면활성제를 이용한 수성 접착제 제조

김상영 ( Sang-young Kim ),이정임 ( Jeong-im Lee ),정노희 ( Noh-hee Jeong ) 충북대학교 산업과학기술연구소 2019 산업과학기술연구 논문집 Vol.33 No.2

Water-based adhesives are increasingly used in recent years because they are easy to handle and environmentally safe. In order to compare the properties of the water-based adhesive according to the surfactant used, synthetic surfactants were confirmed. Solid content, viscosity, initial adhesive force were compared. The synthesized reactive surfactants were polyoxyethylene(10) oleyl ether methacrylate(POE(10)-MA) and polyoxyethylene(20) oleyl ether methacrylate(POE(20)-MA). In the case of using POE(10)-MA and POE(20)-MA respectively, the solid contents were 68% and 56%. The initial adhesion was measured as No. 32 for adhesives with POE(10)-MA and No. 20 for the use of POE(20)-MA, respectively.

무선 통신 시스템에서 성능 향상을 위한 중계 기법

김상영(Kim Sang Young),하창빈(Ha Chang Bin),송형규(Song Hyoung Gue) 한국통신학회 2017 한국통신학회 학술대회논문집 Vol.2017 No.1

ADR의 이념론

김상영(Kim, Sang-Young) 한국재산법학회 2011 재산법연구 Vol.27 No.3

그동안 우리나라 법원 ADR의 현실에 대하여 검토하면서 결과(해결)지향적 관점에서 비롯된 직권조정회부, 수소법원조정, 강제조정의 문제점이 크다는 것을 알게 되었고, 그 근본원인이 이념의 부재에서 비롯된 것이 아닌가라고 생각하게 되었다. 현재 법원의 조정제도 활성화 정책은 비교적 성공적이라고 평가되는 조기조정제도까지 포함하여 문제에 대한 대증치료적인 개선책일 뿐이고, 근본적인 치료방안이라고는 생각되지 않는다. 따라서 조정에 대한 당사자의 신뢰도 등을 고려할 때 법원이 어느 정도 개입하거나 연계된 조정은 현실적으로 필요하다고 생각하지만, 현재의 직권조정회부, 수소법원조정, 강제조정문제에 대하여는 근본적인 재검토가 요구된다고 생각한다. 대체로 조정은 당사자의 신청에 의하여 개시되거나 당사자의 동의를 받아 조정회부되어야 하고, 수소법원과 조정기관은 분리되어 비밀유지원칙이 엄수되어야 하며, 법원이든 외부조정기관이든 강제조정은 가능한 한 자제하도록 하는 것이 올바른 방향이라고 생각한다. ADR이 국민에게 신뢰받고 재판(소송)절차보다 더 많이 이용되도록 하려면, 먼저 ADR의 이념을 바르게 정립하고, 이에 따라 제도와 절차를 새롭게 설계.구축한 후 공정하고 중립적인 제3자가 당사자의 주체성과 자기결정권을 최대한 존중하면서 결과(해결) 지향적이 아니라 과정 지향적으로 관여하여 분쟁당사자에게 새로운 시각을 제공함으로써 인식의 변화를 초래하고 이를 통해 종래 견지해오던 당사자의 태도나 생각을 스스로 바꿀 수 있는 기회를 부여하는 방향으로 운영되는 것이 필요하다고 생각한다. 이렇게 되면 ADR은 재판(소송)절차를 보완하는 성격의 재판(소송)대체적 분쟁해결(처리)제도를 넘어서서 독자적인 분쟁해결(처리)에 가장 적합한 제도가 될 수 있을 것이다. 이에 따라 우리나라 법원 ADR의 현실과 ADR의 이념론 및 ADR의 이념과 관련된 비교법적 고찰을 바탕으로 하여 나름대로 감히 정립을 시도해본 ADR의 이념은 정의, 자율, 덕화(德化), 위민(爲民), 공평, 신속이다. What are the Ideals of ADR? What is the Philosophy of ADR? What is the true spirit of ADR? What is the Motivations for ADR? These are the subjects that I have studied since I took a great interest in ADR. Today, ADR is becoming the preferred choice for the resolution of conflict and disagreement, because ADR processes are usually faster, less expensive, less time-consuming and more conclusive than litigation. In Korea ADR has now become a useful dispute resolution and particularly the court-annexed ADR has produced a good outcome. It might depend primarily, I think, on the 1990 Civil Conciliation Act('CCA'). If mediation is not successful or the respondent is absent on the day of mediation, the judge may render 'compulsory conciliation', which is an authoritative decision of the court (CCA, Article 30). A Party who is prejudiced by such compulsory conciliation can petition to have the conciliation decision nullified within two weeks of the decision. This may result in the party returning to legal proceedings which have previously been filed (CCA, Articles 34 and 36). Alternatively, if there is no objection, the decision has the same effect as a judicial compromise (CCA, Article 29). But the court-annexed ADR has many problems such as substantially compulsory mediation (or conciliation, legally non-binding arbitration) and mediation by a presiding judge that cause many public resentments. The activating and improving countermeasures of the court-annexed ADR with the early mediation that is estimated successful relatively are only symptomatic and do not make the problems fundamentally cured. And so I think the problems (substantially compulsory mediation and mediation by a presiding judge) should be reviewed fundamentally. It is desirable that ADR is started by agreement of the parties. And a presiding judge had better refrain from being a mediator. And compulsory mediation is not appropriate for the ideals of ADR and should be refrained to the utmost. In order to make ADR trusted by people and available more frequently than litigation we must first of all establish the ideals of ADR rightly. The ideals of ADR which I found attentively are Justice, the empowerment of the individual, Moral Influence, Heartfelt Care about parties, Fairness and Speediness. According to these ideals we need to redesign and construct systems and processes other than adjudication by a presiding judge. A fair and neutral third party should respect the empowerment of the individual and the principle of co-operative problem solving hoping at last the preservation or enhancement of personal and business relationships. I wish ADR will be a more appropriate and independent dispute resolution rather than an alternative and assistant dispute resolution through the realization of the ideals of ADR.

단순 해석 모델을 이용한 밸브지지대 내진 성능 평가

김상영 ( Sang-young Kim ),금동엽 ( Dong Yeop Keum ),김형은 ( Hyoung Eun Kim ),김대진 ( Dae Jin Kim ),김준호 ( Jun Ho Kim ),홍성경 ( Seong Kyeong Hong ),최원목 ( Won Mok Choi ),석창성 ( Chang Sung Seok ) 한국안전학회(구 한국산업안전학회) 2017 한국안전학회지 Vol.32 No.1

In this study, a simplified FE model for evaluating seismic performance of valve support was suggested and an apparatus for a real structure testing was developed. The seismic performances of three different types of valve supports were evaluated by the real structure testing. By comparing the results between the real structure testing and FEA using the simplified FE model, it was verified that the suggested simplified FE model can be utilized for comparative evaluation of seismic performance of valve supports.