융모성질환 후 임신에 관한 연구

권혁영,김혜숙,방재희,민보은 대한산부인과학회 1986 Obstetrics & Gynecology Science Vol.29 No.1

폐기물 감량화 및 경제 : MBT를 이용한 생활폐기물의 감량화,연료화에 관한 연구

권혁영,김진우,송선호 한국폐기물자원순환학회(구 한국폐기물학회) 2006 한국폐기물자원순환학회 추계학술발표논문집 Vol.2006 No.-

시판우유 및 유산균제제의 성분 분석

권혁영,이기성,이미경,이예경 이화여자대학교 의학회 1979 梨花醫學會誌 Vol.- No.11

The analytical data of general components and minerals in milk, chocolate milk and yoghurts were as follows; 1) Milk; Water content was about 89.2% and ash 0.73g%, lactose 4.7g%, protein 3.4g%, lipid 2.3g%, iron 0.09mg%, calcium 167.2mg% and magnesium 13.4mg% were obtained. 2) Chocolate milk: Water content was about 87.0% and ash 0.5g%, lactose 6.2g%, protein 2.4g%, lipid 1.4mg%, iron 0.1mg% calcium 118.6mg%, and magnesium 8.1mg% were obtained. 3) Yoghurt: Water content was about 81.2% and ash 0.22g%, lactose 3.7g%, protein 1.1g%, lipid 0.3g%, iron 0.06mg%, calcium 112.0mg% and magnesium 5.4mg% were obtained.

시판우유 및 유산균제제의 성분 분석

권혁영,이기성,이미경,이예경 이화여자대학교 의과대학 1979 梨花醫學誌 Vol.- No.11

The analytical data of general components and minerals in milk, chocolate milk and yoghurts were as follows; 1) Milk; Water content was about 89.2% and ash 0.73g%, lactose 4.7g%, protein 3.4g%, lipid 2.3g%, iron 0.09mg%, calcium 167.2mg% and magnesium 13.4mg% were obtained. 2) Chocolate milk: Water content was about 87.0% and ash 0.5g%, lactose 6.2g%, protein 2.4g%, lipid 1.4mg%, iron 0.1mg% calcium 118.6mg%, and magnesium 8.1mg% were obtained. 3) Yoghurt: Water content was about 81.2% and ash 0.22g%, lactose 3.7g%, protein 1.1g%, lipid 0.3g%, iron 0.06mg%, calcium 112.0mg% and magnesium 5.4mg% were obtained.

위암주위 림프절 및 말초혈액의 림파구아형의 변화에 관한 연구

권혁영,김영진,김현종,김신곤,조영국,정상우 대한외과학회 1992 대한외과학회 학술대회 초록집 Vol.1992 No.11

폐렴구균 알코올탈수소효소의 세포 특이성 및 세포내 분포

권혁영,박연진,표석능,이동권 한국미생물학회 2001 미생물학회지 Vol.37 No.3

열충격 단백질(heat shock protein: HSP)은 변성된 단백질의 응집을 방지하여 가혹한환경에서 병원균의 생존을 증가시킨다. 세균에 알코을 stress를 가하면 다량의 DnaK와 GronEL이 유도되지만 폐렴구균에서는 DnaK와 GroEL이 전혀 유도되지 않는 대신 알코올탈수소효소(alcohol dehydrogenase : ADH)가 유도되었다. 이런 특성은 폐렴구균 ADH가 HSP처럼 chaperone 기능을 수행라고 있을 가능성을 제시하고 있으므로 본 연구에서는 일차적으로 ADH 유전자를 확인하고 ADH 의 면역특성 및 세포내 분포를 측정하였다. 폐렴구균 ADH는 이질아메바 ADH2 및 대장균 ADH 와 높은 유사성을 나타냈으며 883 개의 아미노산으로 구성된 등전점 6.09의 단백질로 추정된다. 그러나 폐렴구균 ADH와 유사성이 높은 대장균, 유산균 및 황색포도상구균의 용해액을 폐렴구균 ADH 항체와 immunoblot을 실시하였을 때 전혀 반응하지 않았다. 또한 세포질, membrane, periplasm에 있는 단백질 분획 및 폐렴구균 배양 상등액을 ADH 항체와 immune blot을 실시하였을 때 ADH 는 열충격에 관계없이 세포 밖으로 분비되는 단백질임을 확인하였다. 이런 결과는 폐렴구균 ADH가 진단용항원 및 백신으로 개발될 수 있는 가능성을 제시하고 있다. Heat shock proteins serve as chaperone by preventing the aggregation of denatured proteins and promote survival of pathogens in harsh environments. In bacteria, ethanol shock induced the major chaperone GroEL and DnaK, but in Streptococcus pneumoniae, it induced neither GroEL nor DnaK but alcohol dehydrogenase (ADH). In this study, ADH gene encoding a 104-kDa (p104) protein was identified and characterized. The deduced amino acid sequence of pneumococcal ADH shows homology with other members of the ADH family, and particularly with Entamoeba histolytica ADH2 and E. coli ADH. S. pneumoniae adh is composed of 883 amino acids and its estimated isoelectric point is 6.09. Although ADH is conserved between S. pneumoniae and E. coli, immunoblot analysis employing antisera raised against pneumococcus ADH demonstrated no cross-reactivity with ADH analog in Eschericha coli, Staphylococcus aureus and human HeLa cells. Also secretion of ADH was demonstrated by subcellular fractionation and immunoblot analysis of proteins. These results suggest that S. pneumoniae ADH could be a highly feasible candidate for both diagnostic marker and vaccine.

밀폐형 ATAD system을 이용한 하수슬러지/음식물쓰레기 통합처리 공정 설계

권혁영,지영환,송한조,김성중,Kwon, Hyeok-Young,Ji, Young-Hwan,Song, Han-Jo,Kim, Seong-Jung 유기성자원학회 2000 유기물자원화 Vol.8 No.4

본 연구에서는 도시고함수율 유기성폐기물(MWWT)의 처리를 위하여, 음식물 쓰레기의 처리에 사용되었던 컨테이너 호기성 퇴비화 시스템과, 계분 및 축분처리에 사용되었던 직립 상향 기계식 교반기를 조합하여 개발하였다. 호기성 처리 공정은 운전이 쉽다는 점과 운전비용이 저렴하다는 점, 자연에서 이용하기 가장 편리한 안정된 부산물을 만들 수 있다는 장점에도 불구하고, 염분의 농축, 과도한 부형제의 사용에 의한 경제적 부담의 증가 및 설비의 대형화 등의 장애물을 가지고 있다. 개발된 공정은 이러한 문제점을 보완하고자 하수슬러지는 초기의 수분조절 및 고속발효를 위해 직립 밀폐형 반응조에서 5일간, 음식물쓰레기는 탈염과 수분조절을 위해 밀폐형 무교반 필터에서, 침출수는 폐수처리 장치에서 처리하였다. 실험결과 국내 퇴비에 품질규격을 만족하면서 부자재의 사용량을 3% 이내로 조절할 수 있어 기존공정의 문제점을 해결할 수 있는 가능성을 확인하였다. In this study, biological treatment process of MWWT(Municipal wet-waste Treatment) has been developed through a moduling of the containerized closed ATAD(Auto thermal aerobic digestion) system & closed vertical dynamic acerator, which were used for food waste and cattle manure, respectively. Though biological process has several advantages such as low concentrations of heavy metals and salts, proper and stable C/N ratio and constant reaction rate against the process treating two wastes separately, it has a obstacles of salt concentration and much usage of bulking agent such as wood chip. After rapid oxidation in the boxed tower reactor for 5 days, the content of sewage sludge would be reduced 65% on around, might be mixed with the food waste that had been treated in the static closed reactor during 6 days and put in the secondary static reactor for curing. During composting process, the odor contained in the gas generated from the reactor was removed by passing it through a biofilter as well as the leachate was treated in the wastewater treatment facility. Consequently, it seemed to be possible to compost sewage sludge at mild and stable operating condition and at low cost through the biological ATAD process resulting in the production of organic compost satisfying the specifications regulated by itself.

시판우유 및 유산군제제의 성분분석

권혁영,이기성,이미경,이예경 梨花女子大學校 醫科大學 1979 梨花醫學誌 Vol.11 No.-

페렴구균 ClpP의 면역 교차 반응과 방어효과

권혁영,이선숙,이순복,표석능,이동권 대한약학회 2004 약학회지 Vol.48 No.1

ClpP is a stress-inducible protein and proteolytic subunit of the ATP-dependent Clp protease in prokaryotes and eukaryotes. Although its physiological roles in bacterial virulence were widely studied in various organsims, including Streptococcus pneumoniae, until now the immunological effect has not been investigated. Here, we have examined the cross reactivity of S. pneumoniae ClpP antibody with other organisms's cell lysate proteins. Although the protein sequence of S. pneumoniae ClpP was highly conserved among various organisms including human, the antibody rasised by S. pneumoniae ClpP was not cross-reacted with other organism's cell lysates, which were Saccharomyces cerevisiae , human lung A549 cell, Bacillus subtilis, Pseuomonas aeruginosa, E. coli, and Salmonella typhi. It was only reacted with S. pneumoniae and Lato-bacillus thermophilus. Thus we examined the immunoprotective effect of ClpP by immunizing mice with the purified ClpP. The mean survival time of mouse was significantly increased with the ClpP immunization. These results suggest that S. pneumoniae ClpP could be used as a vaccine candidate for prevention of S. pneumoniae infection.

Selective Toxicity of Ginsenoside Rg3 on Multidrug Resistant Cells by Membrane Fluidity Modulation

권혁영,Eun-Hye Kim,Seung-Whan Kim,김수남,Jong-Dae Park,이동권 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.2

Multidrug resistance (MDR) is a major problem in cancer chemotherapy. It was previously reported that a red ginseng saponin, 20(S)-ginsenoside Rg3 could modulate MDR in vitro and extend the survival of mice implanted with ADR-resistant murine leukemia P388 cells. This study examined the cytotoxicity of Rg3 on normal and transformed cells, along with its effect on the membrane fluidity. The cytotoxicity study revealed that 120 μM of Rg3 was cytotoxic against a multidrug-resistant human fibroblast carcinoma cell line, KB V20C, but not against normal WI 38 cells in vitro. Flow cytometric analysis using rhodamine 123 as the artificial substrate showed that Rg3 promoted the accumulation of rhodamine 123 in ADR-resistant murine leukemia P388 cells in vivo. Fluorescence polarization studies using the hydrophilic fluorescent probe, DPH, and hydrophobic probe, TMA-DPH, showed that 20 μM Rg3 induced a significant increase in fluorescence anisotropy in KB V20C cells but not in the parental KB cells. These results clearly show that Rg3 decreases the membrane fluidity thereby blocking drug efflux.