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Cheon, Kyeong-Jun,Cho, Seoung-Won,Jang, Won-Seok,Kim, Ju-Won,Yang, Byoung-Eun Korean Association of Maxillofacial Plastic and Re 2020 Maxillofacial Plastic Reconstructive Surgery Vol.42 No.-
Background: Various types of miniplates have been developed and used for the reduction of facial bone fractures. We introduced Yang's Keyhole (YK) plate, and reported on its short-term stability. The purpose of this study was to evaluate the long-term stability of the YK plate, as a follow-up study, by examining the patients who had used the YK plate among the patients with the reduction of mandible fractures and who visited for plate removal. Methods: We reviewed the medical records of 16 patients who underwent mandibular fracture fixation using a YK plate (group I) and 17 patients who underwent mandibular fracture fixation using a conventional plate (group II). Assessment was then made on malunion, occlusal stability, discomfort during the application, and clinical symptoms. Results: From January 2015 to December 2017, a total of 36 patients underwent mandibular fracture surgery using a YK plate. A total of 16 patients received plate removal. Among them, 15 were male and 1 female. The average age was 26 years. The applied surgical sites were the 12 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of YK plate was an average of 335 days. During the same period, 45 people underwent surgery on the conventional plate. A total of 17 patients received plate removal. Among them, 15 were male and 2 females. The average age was 36 years. The applied surgical sites were the 8 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of the conventional plate was an average of 349 days. No malocclusion occurred at the time of removal, and occlusion was stable. No patient complained of joint disease or discomfort. Conclusion: The YK plate system, in which the screw was first inserted and the plate was applied, for clinical convenience did not cause any particular problem and no significant difference from the conventional plate.
Yang, Seung Won,Lee, Seung‐,Min,Choi, Eun Young,Lee, Kyung Hye,Kim, Soo Hyuk,Shin, Min‐,Jeong,Han, Ye Sun,Kang, Seok‐,Min,Chung, Ji Hyung Wiley Subscription Services, Inc., A Wiley Company 2011 Journal of cellular biochemistry Vol.112 No.9
<P><B>Abstract</B></P><P>Cell‐penetrating peptides (CPPs), including TAT‐CPP, have been used to deliver exogenous proteins into living cells. Although a number of proteins fused to TAT‐CPP can be delivered into various cells, little is known about the proteolytic cleavage of TAT‐fusion proteins in cells. In this study, we demonstrate that a small heat shock protein (sHSP), alphaB‐crystallin (αB‐crystallin), delivered by TAT‐CPP is susceptible to proteolytic cleavage by matrix metalloproteinase‐1 (MMP‐1) in cardiac myoblast H9c2 cells. Recombinant TAT‐αB‐crystallin was efficiently transduced into H9c2 cells. For a few hours following protein transduction, generation of a 14‐kDa fragment, a cleavage band of TAT‐αB‐crystallin, increased in a time‐dependent manner. This fragment was observed only in detergent‐insoluble fractions. Interestingly, treatment with MMP inhibitors blocked the cleavage of TAT‐αB‐crystallin. In test tubes, recombinant MMP‐1 processed TAT‐αB‐crystallin to generate the major cleavage fragment 14‐kDa, as observed in the cells treated with TAT‐αB‐crystallin. The N‐terminal sequences of the 14‐kDa fragment were identified as Leu‐Arg‐Ala‐Pro‐Ser‐Trp‐Phe, indicating that this fragment is generated by cleavage at Phe54‐Leu55 of αB‐crystallin. The MMP‐1‐selective inhibitor abolished the production of 14‐kDa fragments in cells. In addition, the cleaved fragment of TAT‐αB‐crystallin was significantly reduced in cells transfected with MMP‐1 siRNA. Moreover, the enzymatic activity of MMP‐1 was markedly increased in TAT‐αB‐crystallin‐treated cells. TAT‐αB‐crystallin has a cytoprotective effect on H9c2 cells under hypoxic insult, moreover, MMP‐1‐selective inhibitor treatment led to even increased cell viability. These results suggest that MMP‐1 is responsible for proteolytic cleavage of TAT‐αB‐crystallin during its intracellular transduction in H9c2 cells. J. Cell. Biochem. 112: 2454–2462, 2011. © 2011 Wiley‐Liss, Inc.</P>
( Yang Won Min ),( So Young Bae ),( Geum Youn Gwak ),( Yong Han Paik ),( Moon Seok Choi ),( Joon Hyoek Lee ),( Seung Woon Paik ),( Byung Chul Yoo ),( Kwang Cheol Koh ) 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.2
Background/Aims: The aim of this study was to identify the parameters that could noninvasively predict the presence of esophageal/gastric varices and portal hypertensive gastropathy (PHG) in patients with chronic liver disease (CLD), and to determine the accuracy of those parameters. Methods: We retrospectively analyzed 232 patients with CLD who underwent both upper endoscopy and liver CT within an interval of 3 months. The multidimensional index (M-Index) for spleen volume was obtained from the multiplication of splenic length, width, and thickness, as measured by computer tomography. Results: The multivariate analysis revealed that platelet, albumin, and M-Index were independently associated with the presence of varices and PHG. We combined three independent parameters, and developed a varices and portal hypertensive gastropathy (VAP) scoring system (=[platelet count (/mm3)×albumin (g/dL)]/[M-Index (cm3)]). The area under the receiver operating characteristic curve of the VAP score was 0.850 (95% confi dence interval, 0.801-0.899). The VAP cut-off value of 861 had a sensitivity of 85.3%, a positive likelihood ratio of 3.17, and a negative predictive value of 86.4%. For predicting high-risk lesions for bleeding, with a cut-off value of 861 the sensitivity was 92.0%, the positive likelihood ratio was 2.20, and the negative predictive value was 96.4%. Conclusions: The VAP score can predict the presence of varices and PHG in patients with CLD and may increase the costbenefi t of screening endoscopy in the clinical practice setting. A prospective validation study is necessary in the future.
( Yang Won Min ),( Seung Woon Paik ),( Geum Youn Gwak,),( Yong Han Paik ),( Moon Seok Choi ),( Joon Hyoek Lee ),( Kwang Cheol Koh ),( Byung Chul Yoo ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background/Aim: Godoxetic acid-enhanced magnetic resonance imaging (MRI) is shown to be superior to computed tomography (CT) in detection and characterization of liver lesions. The objective of the present study was to investigate utility of MRI in surveillance for postoperative recurrence of hepatocellular carcinoma (HCC). Methods: This retrospective study analyzed a total of 147 patients who underwent surveillance with combination of CT and annual godoxetic acid-enhanced MRI after hepatectomy. The subjects in who MRI was performed with suspicion of recurrence from increasing alpha-fetoprotein level were excluded. In this cohort, each HCC recurrence detection rate of MRI and CT was evaluated and recurrent HCC characteristics were compared according to the detection test. Results: Sixty-two patients had recurrent HCC. Among them, 9 were detected with MRI and 29 with CT. In the baseline characteristics of patients with recurrent HCC, there were no significant differences according to the detection test regarding age, gender, etiology of liver disease, platelet count, liver function tests, Child-Pugh class, alpha-fetoprotein level, fibrosis stage, Edmondson-Steiner class, modified UICC stage, and hepatectomy extent. The HCC recurrence detection rates of MRI and CT were 4.8% (9/180) and 4.3% (29/580), respectively on the per test basis (p=0.764). However, in the population with follow-up of ≥12 months, the detection rates of MRI and CT were 4.3% (7/150) and 1.5% (19/400), respectively (p=0.035). The likelihood of HCC recurrence detection in the following surveillance after negative result of MRI or CT was 1.5% (2/133) and 6.0% (36/596), respectively (p=0.033). The recurrent HCCs detected with MRI were smaller than that detected with CT (size <2 cm, 100% vs. 65.5%, p=0.040). Conclusions: Our data suggest that godoxetic-enhanced MRI has a higher detection rate for postoperative HCC recurrence than CT. Surveillance with combination of CT and MRI may identify recurrent HCC at its earlier stage than with CT alone.
( Yang Won Min ),( Nam Jun Kim ),( Geum Youn Gwak ),( Yong Han Paik ),( Moon Seok Cho ),( Kwang Cheol Koh ),( Seung Woon Paik ),( Byung Chul Yoo ),( Joon Hyeok Lee ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background/Aim: The objective of this study was to compare the efficacy of lamivudine (LAM) and entecavir (ETV) in prevention of HBV reactivation in patients receiving systemic chemotherapy. Methods: A total of 554 non-hepatic cancer patients with HBsAg who underwent chemotherapy and received prophylactic antiviral therapy (454 with LAM and 100 with ETV) at the Samsung Medical Center between January 2008 and December 2010 were identified. Exclusion criteria were as following: late prophylaxis (not started within the first cycle of chemotherapy), any prior chemotherapy or/and antiviral therapy, follow-up less than 3 months, irregular medication, and chronic hepatitis B. Finally, clinical outcomes of 146 patients in LAM group and 40 in ETV group were compared. Hepatitis was defined as a 3 times increase in alanine aminotransferase (ALT) that exceeds 40 IU/L or an absolute increase of ALT to >100IU/L when compared with the baseline pre-chemotherapy value. Results: At baseline, there were no significant differences between two groups with regard to gender, aspartate aminotransferase, ALT, bilirubin, albumin, cancer type, purpose of chemotherapy, steroid use, and adjuvant radiotherapy. However, patients were younger (48.2 ± 9.4 vs. 51.5 ± 9.4, p=0.046), HBeAg seropositivity rate was higher (28.9% vs. 11.7%, p=0.010), and baseline HBV DNA level were higher [2401.0 IU/mL (<9->1.7x108) vs. 291.5 IU/mL (<9->1.7x108), p=0.006] in ETV group compared to LAM group. Five patients (2.7%) developed hepatitis: 4/146 (2.7%) and 1/40 (2.5%) in LAM and ETV group, respectively (p=1.000). Among them, 3 patients were accompanied by HBV DNA elevation (≥10-fold) only in LAM group (2.1% vs. 0.0%, p=1.000). During preemptive preemptive treatment, two in LAM group and one in ETV group developed hepatitis (1.4% vs. 2.5%, p=1.000). Severity of hepatitis was not different between two groups. Conclusions: LAM and ETV are comparable in efficacy in terms of prevention of hepatitis in HBsAg-positive patients receiving cancer chemotherapy.
( Won Seok Yang ),( Xiang Zi Han ),( Kyoung Soon Lee ),( Kyung Min Lee ),( Ji Hyun Ju ),( In Cheol Shin ) 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.1
In this study we present a mammalian cell culture model that allows to study the effect of endocrine disruptors (EDCs) on aromatase activity of aquatic amphibian, Bombina orientalis. Bombina orientalis aromatase gene was subcloned into a mammalian expression vector and subsequently transfected to mammalian cells. Although the protein expression level of Bombina orientalis aromatase was low, it had a significant aromatase activity. When EDCs were added to aromatase transfected cells, aromatase activity was significantly decreased. We report here that this system may be used to monitor the effect of EDCs on aromatase activity of aquatic organisms.