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고착협 내탈립 기계수확 적응 장류⋅두부용 콩 품종 ‘새금’
김현태(Hyun Tae Kim),한원영(Won Young Han),이병원(Byung Won Lee),고종민(Jong Min Ko),이영훈(Yeong Hoon Lee),백인열(In Youl Baek),윤홍태(Hong Tai Yun),하태정(Tae Joung Ha),최만수(Man Soo Choi),강범규(Beom Kyu Kang),김현영(Hyun Yeong K 한국육종학회 2019 한국육종학회지 Vol.51 No.4
The soybean cultivar, ‘Saegeum’, has been developed for preparing soy-paste and tofu. The soybean cultivars ‘Daepung’ and ‘SS98207-3SSD-168’ were crossed in 2003 to obtain ‘Saegeum’. Single seed descent method was used to advance the generation from F3 to F5, and the plant lines with promising traits were selected from F6 to F7 by pedigree method. The preliminary yield (PYT) and advanced yield trials (AYT) were conducted from 2009 to 2010, and the regional yield trial (RYT) was conducted in 12 regions between 2011 and 2013. The morphological characteristics of ‘Saegeum’ were as follows: determinate plant type, white flower, tawny pubescence color, and brown pod color. Flowering and maturity dates were August 2, XXXX and October 17, XXXX, respectively. Plant height, first pod height, number of nodes, number of branches, and number of pods were 79 cm, 18 cm, 16, 2.3, and 44, respectively. The seed characteristics of ‘Saegeum’ were as follows: yellow spherical shape, yellow hilum, and the 100-seed weight was 25.4 g. ‘Saegeum’ was resistant to bacterial pustule and SMV in the field test, and its lodging resistance was mildly strong, whereas its shattering resistance was excellent. The ability of this cultivar to be processed into tofu, soybean malt, and other fermented products was comparable with that of ‘Daewonkong’. The yield of ‘Saegeum’ in the adaptable regions was 3.02 ton ha-1. Thus, ‘Saegeum’ is adaptable to mechanized harvesting because of its high first pod height, as well as lodging and shattering resistance. (Registration number: 5929)
Thioacetamide에 의한 BALB/c 마우스 간의 시간별 약물대사효소 억제 양상 : A Time-Course Study
이정운,고우석,김갑호,배연경,하현정,한상섭,천영진,정태천 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-
Thioacetamide is a potent hepatotoxicant which requires metabolic activation by cytochrome P450s (P450s) for toxicity. In the present study, the elevation kinetic of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by thioacetamide treatment was investigated in male BALB/c mice. Inaddition, the inhibitory effects of thioacetamide on liver microsomal P450 enzymes were further investigated. Thioacetamide at 100 mg/kg/ was treated intraperitoneally for 6, 12, 24, 36, 48 and 72 hr. The blood was collected at the designated time for assaying the serum enzyme activities. To determine the P450 isozyme-specific activities. ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and benzyloxyresorufin O-debenzylase (BROD) activities were determined for P450 1A1, 1A2 and 2B1, respectively, in liver microsomal fractions. The activities of ALT and AST were started to be elevated 6 hr after thioacetamide treatment andreached the maximun at 36 hr after the treatment. The elevated activities were dramatically recovered at 72 hr. The microscopic exmination of the liver specimen also showed a similar profile of hepatotoxicity. All P450-associated enzyme activities were time-dependently inhibited by the treatiment with thioacetamide. The maximum inhibition of P450 enzymes was observed 36 hr after the treatment. Because the inhibition of P450 enzymes by thioacetamide was time-dependent, our present results suggest that thioacetamide might inhibit P450 enzymes in mechanism-based inactivation.
Oxidation of Rosmarinic Acid Catalyzed by Mushroom Tyrosinase
Tae Joung Ha,Myoung-Hee Lee,Hyun Sook Kwon,Byeong Won Lee,Chang-Hwan Park,Suk-Bok Pae,Chan-Sik Jung,Keum-Yong Park 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Rosmarinic acid isolated from the Perilla frutescens (L.) was found to possess mushroom tyrosinase (EC 1.14.18.1) inhibitory activities with an IC50 of 4.0 μM. However, rosmarinic acid was oxidized as a substrate catalyzed by tyrosinase. The oxidation kinetics of mushroom tyrosinase by rosmarinic acid measured by spectrophotometic and polarographic methods showed Km=166.8 μM, 225.1 μM and Vmax=16.26 μM/min, 3.05 mg O2/L/min, respectively.
Tae Joung Ha,Myoung-Hee Lee,Chang-Hwan Park,Suk-Bok Pae,Kang-Bo Shim,Chung-Dong Hwang,Young-Sil Kim,Byeong-Won Lee,Keum-Yong Park 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
The influenza neuraminidase (E.C. 3.2.1.18) is an antiviral target of high pharmaceutical interest because of its essential role in cleaving sialic acid residues from surface glycoproteins and facilitating release of virions from infected cell. In this context, polyphenolic compounds including luteolin, rosmarinic acid, and apigenin from Perilla frutescens were evaluated for their inhibitory effect on recombinant virus H1N1 neuraminidase. Among the test compounds, luteolin and rosmarinic acid inhibited the rvH1N1 neuraminidase with an IC50 of 8.4 and 46.7 μM, respectively. The inhibition kinetics analyzed by Dixon plots indicates that luteolin and rosmarinic acid are noncompetitive inhibitor and inhibition constant, Ki, were established as 14.3 and 43.9 μM, respectively. Subsequently, we also analyzed the rosmarinic acid and luteolin contents of 383 accessions of perilla seed germplasms by high performance liquid chromatography (HPLC). The rosmarinic acid and luteolin contents of perilla seeds were ranged from 15.7 μg/g to 2,894.9 μg/g and from 1.6 μg/g to 949.1 μg/g, respectively.
Isolation of triterpene soyasapogenol A and B and it's distribution of soybean cultivars
Tae Joung Ha,Jong-Min Ko,Chan-Sik Jung,Sang-Ik Han,Myoung-Hee Lee,Yu-Na Jeong,Keum-Yong Park 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
This study was carried out to investigate the variation of triterpene soyasapogenol A and B in soybean cultivars. Soyasapogenol A and B were isolated from acid hydrolysis of 80% aqueous ethanol extract of soybean and the structures of these soyasapogenols were characterized by 1H and 13C NMR analysis. And the distribution of soyasapogenol A and B were determined by high-performance liquid chromatography (HPLC) with variable wavelength detector (VWD). The soyasapogenol A and B contents of soybean cultivars ranged form 49.9 ㎍/g (Geomjeongsaeol) to 701.5㎍/g (Sorog) and from 91.4㎍/g (Danmi) to 2,315.9 ㎍/g (Daewonkong), respectively. Sorog cultivar showed the highest total soyasapogenol contents (2,773.8 ㎍/g), whereas Geomjeongkong 3 cultivar displayed the lowest (83.5㎍/g). The average content ratio of soyasapogenol B (1,061.4㎍/g) was significantly higher than that of soyasapogenol A (292.5 ㎍/g).
Tae Joung Ha,Chan-Sik Jung,Myoung-Hee Lee,Yu-Na Jeong,Dae-Sik Choi,Keum-Yong Park 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Anthocyanins of black soybean may play an important role in physiological functions related to human health such as antioxidant, anti-inflammatory, and anticancer. The objective of this study was to investigate the profiles of anthocyanin in fourteen black soybean varieties using high-performance liquid chromatography (HPLC) with photodiode array detector (PAD). In all of the cultivars analyzed totally nine anthocyanins including, CatCy3glc, Dp3gal, Dp3glc, Cy3gal, Cy3glc, Pt3glc, Pg3glc, Pn3glc, and Cy were found. Cy3glc was the major anthocyanin content, represention 69.5% of anthocyanin, followed by Dp3glc (23.0%), Pt3glc (4.9%), Pn3glc (1.2%), and Pg3glc(1.1%), respectively. In contrast, the other five anthocyanins were in very low amounts, below 0.3 % in all varieties. Comparing cultivars and anthocyanin compositions, Geomjeong 2, Cheongja 2, and Cheongja 3 were found to content 9 anthocyanins. Geomjeong 1 and Seonheuk contented CatCy3glc, Cy3gal, Cy3glc, and Pn3glc. The variation of total anthocyanin concentration were significant for soybean cultivars. Geomjeong 2 showed highest average total anthocyanin content (17,937.8 ㎍/g seed coat), and Tawonkong and Heugcheong had the lowest total anthocyanin values (2,835.7 and 2,853.1 ㎍/g seed coat, respectively).
Cytotoxic Effects of Sesquiterpene Lactones from the Flowers of Hemisteptia Iyrata B.
Ha, Tae-Joung,Jang, Dae-Sik,Lee, Jong-Rok,Lee, Kyung-Dong,Lee, Jun,Hwang, Seon-Woo,Jung, Hwa-Jin,Nam, Sang-Hae,Park, Ki-Hun,Yang, Min-Suk The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.11
Four guaia-12,6-olide type sesquiterpene lactones, aguerin B (1), 8$\alpha$-acetoxyzaluzanin C (2), cynaropicrin (3), and deacylcynaropicrin (4), were isolated from the flowers of Hemisteptia Iyrata Bunge. It is the first report on the isolation of compounds 1-4 from Hemisteptia species. All the isolates (1-4) were examined for their cytotoxic activity against SK-OV-3, LOX-IMVI, A549, MCF-7, PC-3, and HCT-15 human cancer cell lines.
Oxidation of Rosmarinic Acid Catalyzed by Mushroom Tyrosinase
Ha, Tae-Joung,Lee, Myoung-Hee,Kwon, Hyun-Sook,Lee, Byeong-Won,Park, Chang-Hwan,Pae, Suk-Bok,Jung, Chan-Sik,Park, Keum-Yong The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Rosmarinic acid isolated from the Perilla frutescens (L.) was found to possess mushroom tyrosinase (EC 1.14.18.1) inhibitory activities with an $IC_{50}$ of $4.0{\mu}M$. However, rosmarinic acid was oxidized as a substrate catalyzed by tyrosinase. The oxidation kinetics of mushroom tyrosinase by rosmarinic acid measured by spectrophotometic and polarographic methods showed $K_m=166.8{\mu}M$, $225.1{\mu}M$ and $V_{max}=16.26{\mu}M$/min, 3.05 mg $O_2$/L/min, respectively.
Ha, Tae Joung,Bo Song, Seok,Ko, Jeeyeon,Park, Chang-Hwan,Ko, Jong-Min,Choe, Myeong-Eun,Kwak, Do-Yeon,Lee, Jin Hwan Elsevier 2018 Food chemistry Vol.266 No.-
<P><B>Abstract</B></P> <P>The α-glucosidase inhibition effects of the 80% ethanol extracts in the seeds of five <I>Vigna</I> species (<I>V. nakashimae</I>, <I>V. nipponensis</I>, <I>V. umbellate</I>, <I>V. radiate</I>, and <I>V. angularis</I>) were evaluated and their half-maximal inhibitory concentration (IC<SUB>50</SUB>) values showed considerable differences (<I>p</I> < 0.05) ranging from 7.3 to >900 μg/ml. <I>V. nakashimae</I> exhibited the most potent inhibition with IC<SUB>50</SUB> value of 7.3 ± 1.1 μg/ml, followed by <I>V. nipponensis</I> (184.0 ± 9.5 μg/ml) and <I>V. umbellata</I> (520.0 ± 8.1 μg/ml). Bioactivity-guided fractionation of <I>V. nakashimae</I> seeds yielded three phenolics by silica gel chromatography and their structures were elucidated as gambiriin D (<B>1</B>), luteoliflavan-7-<I>O</I>-glucopyranoside (<B>2</B>), and catechin-7-<I>O</I>-glucopyranoside (<B>3</B>) using nuclear magnetic resonance (NMR) spectroscopy. In particular, gambiriin D (<B>1</B>) possessed strong inhibition activity with IC<SUB>50</SUB> of 36.8 ± 2.3 μM through simple reversible slow-binding inhibition (kinetic parameters: <I>k</I> <SUB>4</SUB> = 0.0048 μM<SUP>−1</SUP>s<SUP>−1</SUP>; <I>K</I> <SUB>i</SUB> <SUP>app</SUP> = 48 μM). Furthermore, this compound inhibited recombinant human aldose reductase with IC<SUB>50</SUB> value of 12.0 ± 0.7 μM. Results suggest that <I>V. nakashimae</I> may be a potent α-glucosidase inhibition for health products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>V. nakashimae</I> seeds showed potent α-glucosidase inhibition (IC<SUB>50</SUB> = 7.3 ± 1.1 μg/ml). </LI> <LI> Three phenolic compounds were identified from the seeds of <I>V. nakashimae</I>. </LI> <LI> Gambiriin D inhibited α-glucosidase with reversible slow-binding mode (IC<SUB>50</SUB> = 36.8 ± 2.3 μM). </LI> <LI> Gambiriin D inhibited human aldose reductase with IC<SUB>50</SUB> value of 12.0 ± 0.7 μM. </LI> </UL> </P>