연구논문 : 자연과학 ; 남부지역에서 비닐피복에 따른 콩 생육특성 및 수량성 차이

백인열 ( In Youl Baek ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.1

Nowadays P. E. film mulching for weed control and moisture preservation is popular in soybean cultivation of southern region of Korea. Some merits of P. E. film mulching are moisture preservation, soil aggregation, weed control and growth promotion due to increase soil temperature. The rearrangement of seeding date of soybean is necessary to prevent overgrowth of soybean and promote healthy growth. As global warming increases average world temperature, the demanding of re-establishing soybean cultivation system, such as seeding date, has been increased. Therefore, we discussed seeding date accompanying maturity types as soybean was cultivated on condition of P. E. film mulching. Four varieties, Saeol, Seonyu, Daewon and Pungsannamul were seeded on May 10, May 25, June 10 and June 25 in P. E. film mulching and non-mulching field. It is required more mean days of flowering in nonmulching and more days of maturity in P. E. film mulching. In R5 stage completed organ structure dry matter weight was heavier and LAI was ranged 5 to 7 which was optimum LAI. Number of pods and number of seeds per plant in P. E. film mulching were higher on June 25 seeding and 100-seed weight was heavier through all seeding date of P. E. film mulching. Seed yield in P. E. film mulching cultivation was higher on May 25 and June 10 seeding. Occurrence of damaged seeds showed no difference between two cultivation types and among seeding dates. Major diseases and insect pests were bacterial pustule, black root rot, wild fire and stink bugs. And that, occurrence of root rot and stink bugs influenced to yield decrease. Protein content of soybean seed in P. E. film mulching was lower in all seeding dates. Fatty acid compositions showed no difference between two culivation types and among seeding dates. But C18:1 composition was higher on May 10 and May 25 seeding in P. E. film mulching cutivation and C18:3 composition was similar between two cultivation types. Isoflavone content was higher in P. E. film mulching cultivation and higher as seeding date was delayed. Summerizing above results optimum seeding date for P. E. film mulching cultivation ranged from May 25 to June 10 in southern region of Korea.

연구보문 : 콩 비닐피복 재배시 최적적심방법 연구

신상욱 ( Sang Ouk Shin ),김현태 ( Hyun Tae Kim ),이영훈 ( Young Hoon Lee ),정찬식 ( Chan Sik Jeong ),이병원 ( Byong Won Lee ),고병구 ( Byong Gu Ko ),백인열 ( In Youl Baek ),박금룡 ( Keum Yong Park ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.4

콩의 비닐피복 재배시에 최적적심 방법을 구명하고자 본 시험에서 적심방법별 주요 특성 및 수량 비교한 결과는 다음과 같다. 1. R5 stage의 건물중은 두 품종 모두에서 V8 stage의 순적심과 개화시 적심에서 많았는데 이들 처리는 대원콩에서는 무처리 보다 가벼웠고 신부석태에서는 무처리 보다 무거웠다. 그리고 이들 처리는 최적엽면적 지수을 확보하였다. 2. 포장도복은 V8 stage의 순적심에서는 발생하지 않았고 개화시 적심에서는 2~5 정도의 도복이 발생하였다. 3. 개화기는 품종별 처리간 모두 같았고, 성숙기는 대원콩에서 1~2일의 차이를 보였지만 신부석태는 차이가 없었다 4. 종실비대기간인 R5 stage에서 R7 stage까지의 두 품종 평균 순동화량(NAR)은 V8 stage의 순적심과 개화시 적심에서 비슷하면서 가장 높았다. 5. 두 품종 평균수량은 V8 stage의 순적심과 개화시 적심에서 가장 높았다. 6. 외관적 품질에서는 V8 stage의 순적심에서 백립중이 가장 무거웠고 미숙립 및 다른 피해립율도 낮았다. This experiment was carried out to make clear optimum decapitation method for yield increase of soybean and to reduce lodging as soybean is cultivated in vinyl mulching. Results are as follow; In R5 stage, dry matter weight in two varieties was higher in V8 (terminal) treatment and flowering initiation stage treatment which were lighter than control in Daewon and heavier in Sinbuseoktae. Also, LAI(Leaf Area Index) in these two treatments was suitable. Lodging index in V8 (terminal) treatment was not occurred whereas in flowering initiation stage treatment was ranged from 2 to 5. Flowering date in two varieties was not differant among five treatments and maturity date was similar. NAR (Net Assimilation Rate) from R5 to R7 stage was higher in both V8 (terminal) and flowering initiation stage treatment showing similar mean value. Although mean values was not significantly different, the yield of terminal decapitation in V8 stage which was similar to flowering initiation stage treatment was higher. Also, 100-seed weight of terminal decapitation in V8 stage was the heaviest and apparent seed quality was favorable. Consequently, the optimum decapitation method in vinyl mulching cultivation of soybean was terminal decapitation in V8 stage.

풋콩 수확 전후의 당 및 토코페롤 변화 양상

고종민 ( Jong Min Ko ),하태정 ( Tae Joung Ha ),김현태 ( Hyun Tae Kim ),한원영 ( Won Young Han ),이병원 ( Byeong Won Lee ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),백인열 ( In Youl Baek ) 한국콩연구회 2011 韓國콩硏究會誌 Vol.28 No.1

Soybeans undergo many compositional changes during the seed filling stage, and green vegetable soybeans also occur the changes before and after harvest. First experiment was carried out to investigate the changing patterns of sugars of vegetable soybean by storage temperature and period after harvest. When green pods of vegetable soybeans were stored in 25˚C after harvest, the sucrose contents were drastically decreased above 60 percent from 8.7~10.4% to 3.0~3.1% only a day after harvest. On the other hand, when vegetable soybeans were stored in -20˚C immediately after harvest, the sucrose contents have maintained 86.5~90.8% in eleven days after harvest compared with the content of harvesting day, and in 4˚C have kept 75.9~79.8%. These results show that green pods of vegetable soybean must keep in low temperature on the day of harvest to improve a sweet taste. In second experiment, the changing patterns of tocopherol in immature soybean were analyzed using 3 cultivars, Hwaeumput, Danmi and Danmi2. Danmi2 having the highest content was 435.5 μg/g in 35 days after flowering. The contents of total tocopherol in 45 days were decreased to 43.6% in Hwaeumput, 42.4% in Danmi and 68.7% in Danmi2 respectively, compared with the content of 35 days. Even if the decreasing patterns were similar among 3 cultivars, Danmi2 showed the highest content and the slowest reduction. These results imply that it is important the choice of variety for production of vegetable soybean possessing high tocopherol.

RAPD方法에 의한 콩屬의 種間 類緣關係 分析

In Youl Baek(白寅烈),Yong Hwi Yoon(尹用輝),Dao Chull Shin(申斗澈),Gyu Hwan Park(朴圭煥),Young Hun Hwang(黃永鉉),Dal Ung Kim(金達雄) 한국육종학회 1997 한국육종학회지 Vol.29 No.3

Effective conservation and the use of plant genetic resources are essential for future agricultural progress. In this study, the genetic relationships among fourteen species of Glycine were analyzed based on the RAPDs. A total of fourteen species (twenty one accessions) in the genus Glycine were identified by the frequency of RAPDs polymorphisms. Ten primers out of thirty primers tested, were selected for the reliability and repeatability of banding patterns. The amplification of the genomic DNA produced eighty two polymorphic band patterns with the average of 8.2 bands per primer. By Nei and Li’s formula, the mean 1-F value (genetic similarity) was 0.461, and the highest value was 0.774 (G. max : G. microphylla) and the smallest one was 0.128 (intraspecies G. max). That of interspecies in the subgenus Glycine was 0.620 and 0.250 in the highest and the lowest cases, respectively. Thus the genetic similarities were much variable within the interspecies of subgenus Glycine but those were somewhat small between the subgenus Glycine and Soja. By UPGMA (unweighted pair group method using an arithmetic average) cluster analysis based on 1-F value, the fourteen species of Glycine could be classified into four major-groups (genetic similarity (GS) : 0.45) and nine sub-groups (GS : 0.35), that is, into Group Ⅰ : subgenus Soja : G. max, G. sofa, G. gracilis; Group Ⅱ : G. falcata ; Group Ⅲ : A : G. latrobeana, G. canescens, G. microphylla, B : G. curvata, G. cyrtoloba, C : G. clandestina, D : G. tabacina(2n=80) ; Group Ⅳ : A : G. arenaria, B : G. tomentella (2n=38, 40, 78, 80), C : G. tabacina (2n=40, 120), G. latifolia. By multidimensional scaling (MDS), the subgenus Glycine and Soja were clearly classified in the clustering of genetic relationship distance. The genetic relationship distance was closely identified between G. max of the cultigen and G. clandestina, G. tomentella, G. cyrtoloba, G. curvata of the subgenus Glycine.

장류용 내병 내도복 다수성 콩 신품종 ‘대하 1호’

백인열(In-Youl Baek),김현태(Hyun-Tae Kim),고종민(Jong-Min Ko),한원영(Won-Young Han),박금룡(Keum-Yong Park),오기원(Ki-Won Oh),하태정(Tae-Joung Ha_,신상욱(Sang-Ouk Shin),윤홍태(Hong-Tae Yun),문중경(Jung-Kyung Moon),오영진(Young-Jin Oh) 한국육종학회 2013 한국육종학회지 Vol.45 No.1

A new soybean cultivar for soy-paste, ‘Daeha 1’, was developed by soybean breeding team in the Yeongnam Agricultural Research Institute (YARI) in 2008. A promising line, SS97214-S-S-S-15, was selected from the combination between ‘Suwon192’ and a pedigree came from cross combination between ‘Jangyeobkong’ and ‘Hwaeomputkong’. It was designated as the name of ‘Milyang 164’. It had good result from regional adaptation yield trial (RYT) for three years from 2006 to 2008 and released as the name of ‘Daeha 1’. It has a determinate growth habit, white flower, grey pubescence, yellow seed coat, yellow hilum, large spherical seed (25.4 grams per 100 seeds). ‘Daeha 1’ is tolerant to soybean mosaic virus and bacterial pustule, the major soybean disease in Korea. The average yield of ‘Daeha 1’ was 2.62 ton per hectare in the regional yield trial (RYT) carried out for three years from 2006 to 2008, which was 5 percent higher than that of check cultivar, ‘Taekwangkong’.

국내 콩에서 발생하는 바이러스 병해의 발생 상황

이영훈 ( Yeong Hoon Lee ),임승택 ( Seung Taek Lim ),윤영남 ( Young Nam Yoon ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),고종민 ( Jong Min Ko ),이수헌 ( Su Heon Lee ),이기운 ( Key Woon Lee ),백인열 ( In Youl Baek ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.29 No.1

국내 콩에서는 SMV, SbDV, AMV, CMV, CPMV, SYMMV, SYCMV와 PSV가 보고되었다. 과거 이들 바이러스 중에서 SMV가 심각한 피해를 입히고 있으며, 90%이상 우점하는 것으로 보고되었다. 하지만, 최근 SMV의 발생률은 50%정도로 낮아졌으며, SYMMV, SYCMV와 PSV 등에 의한 여러 가지 바이러스 병해가 피해를 주고 있는 것으로 확인되었다. 최근 기후 및 재배환경의 변화와 국제 농산물 교역으로 인해 병 발생 양상이 급속하게 변하고 있음을 나타내는 단적인 예인 것이다. 또한, 새로운 매개층과 전염원의 발생으로 신종 또는 미보고 바이러스 발생이 늘어나고 있지만, 콩과 같은 주요 작물의 병해 발생 상황 및 피해 양상 구명에 대한 연구는 미흡한 실정이다. 이러한 이유로 국내 콩에서 발생하는 바이러스 병해의 확인을 위해 8도19지역에서 193점의 시료를 채집하였다. 채집된 시료들은 SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV와 CPMV 14종에 대한 정밀 진단을 위하여 각각의 종특이 프라이머를 이용하여 RT-PCR 진단이 수행되었다. 그 결과 채집된 시료의 86%가 바이러스에 감염된 것으로 확인 되었으며, SMV 141, SYMMV 14, PSV 8과 SYCMV 5점이 확인되었다. 대구와 나주에서 채집된 2점의 시료들에서는 SYMMV와 SYCMV가 복합감염 되어있었다. 나머지 미동정 시료에 대한 정밀 분석과 지속적인 발생상황 조사가 이루어 져야 할 것이다. It had been reported that soybean viral diseases are Soybean mosaic virus (SMV), Soybean dwarf virus (SbDV), Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Cowpea mosaic virus (CPMV), Soybeun yellow rnottle mosaic virus (SYMMV), Soybean yellow common mosaic virus (SYCMV) and Peanut stunt virus (PSV) in Korea. Among these viral diseases, SMV caused severe damage to soybean in Korea, Although SMV occurred more than 90% in the past, recently several viruses such as SYMMV, SYCMV, SbDV and PSV have been reported in Korea, It means that the incidence of viral diseases are changing in soybean. To identify the viruses infecting soybean in Korea, the 193 samples with viral symptoms were collected in 19 areas of 8 provinces. And then the RT-PCR assay was conducted to detect 14 different viruses such as SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV and CPMV. The results indicated that about 86% of samples were identified as Virus-infected, Among 193 soybean samples, 141 SMV, 8 PSV, 14 SYMMV, 5 SYCMV were detected. Two samples were coinfected with SYMMV and SYCMV. The rest of them were likely to express the mosaic similar to virus-induced symptoms. Therefore, the identifications of the unknown samples have been performed by the dererminatlon of the nucleotide sequences of the genomic RNAs.

경남지역에 있어 장류콩(선유콩,대원콩)의 적정파종시기

신상욱 ( Sang Ouk Shin ),김현태 ( Hyun Tae Kim ),한원영 ( Won Young Han ),이병원 ( Byung Won Lee ),박현진 ( Hyun Jin Park ),고종민 ( Jong Min Ko ),백인열 ( In Youl Baek ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.2

This experiment was conducted to investigate an optimum seeding date of soybean for soy sauce and tofu in Kyungnam region for 2009~2011. Soybean seeds of Seonyu(quasi-early maturity) and Daewon(mid-late maturity) were sown from May 10 to June 25 at the 15-day interval, and dry matter production, disease occurrence, lodging and soybean yield were evaluated. Days to vegetative and reproductive growth in two varieties became longer as seeding date was early. Dry matter weight per unit area in R5 stage of Seonyu was the heaviest at the June 10 seeding and all seeding dates produced optimum range of leaf area index. Dry weight of Daewon in R5 stage was heavier as seeding date was earlier while leaf area index reached to optimum except the June 25 seeding. The ratio of sink/source in Seonyu and Daewon was lowest at the May 25 seeding date, which meaned the lowest load of leaf to pod. Yield of Seonyu was not significantly different among the seeding dates. Yield of Daewon was also significantly high at the May 25 seeding as compared with other seeding dates. The low yield of Daewon at the June 10 seeding was due to severe occurrence of diseases and black root rot in 2009 and bacterial pustule for 2009~2011. This result indicates that optimum seeding date of soybean for soy sauce and tofu was shown to be late May~early June in Kyungnam region.

콩에 발생하는 세균병의 종자 오염 및 불마름병과 들불병 저항성 평가

이영훈 ( Yeong Hoon Lee ),전명기 ( Myeong Gi Jeon ),최만수 ( Man Soo Choi ),강범규 ( Beom Kyu Kang ),김현영 ( Hyun Young Kim ),윤홍태 ( Hong Tae Yun ),백인열 ( In Youl Baek ),윤영남 ( Young Nam Yoon ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.30 No.1

It has been reported that bacterial diseasees of domestic soybean are bacterial pustule (Xanthomonas axonopodis pv. glycines), wildfire ( Pseudomonas syringae pv. tabaci), bacterial blight (Pseydomonas savastanoi pv. glcines) and bacterial brown spot (Pseudomonas syringae pv. syringae) in Korea. Bacterial pustule had been the most issue in soybean diseases. In recently wildfire also occurrence in soybean and Bacterial blight and bacterial brown spot have been reported to less incidence in Korea. Each bacterial diseases were seed borne and then they caused diseases as orumary casual agent. in this study, direct OCR assay was applied to detect casual agent of bacterial diseases and survey on seed contamination on 44 soybean cultivars and 14 genctic resources in Korea, The pathogens were detected frin the seed samples incubated for 12h with 160rpm shaking. The result of surbey on seed contamination showed that bacterial pustule, wildfire and bacterial blight were detected from some cultivars and genetic resources. We also conducted resistance screening in other to select resistance cultivars of Baterial pustule and Wildfire. we inoculated on 128 cultivars with Baterial pustule and Wildfire respectively. These cultivars were classified through pathoggenecity from 1 to 9. In addition, 35 and 50 resistant cultivars were confirmed against Baterial pustule and Wildfire respectively

대립 내탈립 녹색종피 유색콩 ‘청미인’

서정현(Jeong Hyun Seo),한원영(Won Young Han),고종민(Jong Min Ko),백인열(In Youl Baek),이병원(Byong Won Lee),윤홍태(Hong Tai Yun),이영훈(Young Hoon Lee),신상욱(Sang Ouk Shin),오기원(Ki Won Oh),하태정(Tae Joung Ha),최만수(Man Soo Choi) 한국육종학회 2021 한국육종학회지 Vol.53 No.3

‘Cheongmiin’ is a green seed-coated soybean cultivar developed from a cross between ‘Cheongjakong’ and ‘Daemang’ in 2002. The F₁ and F₂ populations were grown for 2 years, and promising lines were selected based on the pedigree method from generations F₃ to F₅. Preliminary and advanced yield trials were conducted from 2009 to 2010, and regional yield trials (RYTs) were conducted in eightregions from 2011 to 2013. ‘Cheongmiin’ is a determinate soybean with white flowers, green cotyledons, and spherical green seeds. Theaverage flowering and maturing dates of ‘Cheongmiin’ were August 1st and October 22nd, respectively. In terms of quantitative characteristics,‘Cheongmiin’ has a larger seed size (34.3 g/100-seed weight) than that of ‘Cheongdu1ho’ (24.5 g/100-seed weight), and has also been shownto be tolerant to lodging and pod shattering in the field in RYTs and oven drying tests, respectively. Furthermore, ‘Cheongmiin’ has beenfound to be resistant to bacterial pustules in the field and soybean mosaic viruses (G5H and G6H strains) in inoculation tests. The meanyield of ‘Cheongmiin’ in the RYTs was 272 kg/10 a, which was 4% higher than that of ‘Cheongdu1ho’. Given its large seed size and seedcoat color, ‘Cheongmiin’ is expected to be highly useful for cooking with rice and for the preparation of rice cakes (Registration No. 7688).

大豆屬(Glycine spp.)의 形態的 特性에 의한 類緣關係 分析

In Youl Baek(白寅烈),Doo Chull Shin(申斗澈),Hyun Tae Kim(金賢泰),Hyung Soo Suh(徐亨洙),Yong Ho Kwack(郭龍鎬),Yun Jin Oh(吳潤鎭) 한국육종학회 1997 한국육종학회지 Vol.29 No.2

Discovery and transfer of useful characters of wild species into the elite lines may facilitate the development of crop varieties with broad genetic bases. In this study, morphological relationships among fourteen Glycine species was analyzed on basis of twenty-five morphological characters. The upper five principle components contracted from twenty-five morphological characters by the principle component analysis accounted for about 72% of the total variation. The subgenus Soja; G. max, G. gracilis, and G. soja were clearly seperated from the subgenus Glycine; G. arenaria, G. falcata, G. canescens, G. latifolia, G. microphylla, and G. tomentella by the scatter diagrams distributed on the plane of the first and second or the first and third principle components. The cluster analysis and morphological characteristics of G. cyrtoloba and G. curvata were very similar, but they are distinguished from tri-compound leaves at the unifoliate leaf node. Fourteen Glycine species could be classified into eight major groups at the average distance of 0.75 and fourteen sub-groups at the average distance of 0.45, that is, into Group Ⅰ: G. max; Group Ⅱ: A: G. gracilis, B: G. soja; Group Ⅲ: G. microphylla; Group Ⅳ: G. tomentella (2n=38, 40, 78, 80) ; Group Ⅴ: A: G. tabacina (2n=120), B: G. tabacina(2n =40, 80) ; Group Ⅵ: A: G. clandestine, B: G. canescens; Group Ⅶ: A: G. latifolia, B: G. tabacina (2n=40), G. latrobeana, C: G. cyrtoloba, G. curvata; and Group Ⅷ: A: G. falcata, B: G. arenaria.