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Two Androstane Derivatives from the Cultures of Fungus Marasmiellus ramealis (Bull.) Singer
Ning-Ning Yang,Qing-Yun Ma,Sheng-Zhuo Huang,Hao-Fu Dai,Zhi-Kai Guo,Zhi-Fang Yu,You-Xing Zhao 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.11
A new androstane derivative, 4β-methyl-15-oxa-14β-androstane-7-ene-4α-carboxylic acid (1) and a known one 4β-methyl-15-oxa-14β-androstane-7-ene-4α-hydroxyl (2) were isolated from the EtOAc extract of the cultures of the fungus Marasmiellus ramealis (Bull.) Singer. Their structures were elucidated on the basis of 1D and 2D NMR as well as MS spectroscopic data analysis. The inhibitory activity of two isolates against acetylcholinesterase (AChE) revealed that compound 1 exhibited definitely inhibitory activity.
Zhang, Qing-Mei,Shen, Ning,Xie, Sha,Bi, Shui-Qing,Luo, Bin,Lin, Yong-Da,Fu, Jun,Zhou, Su-Fang,Luo, Guo-Rong,Xie, Xiao-Xun,Xiao, Shao-Wen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
Melanoma-associated antigen (MAGE) family genes have been considered as potentially promising targets for anticancer immunotherapy. MAGED4 was originally identified as a glioma-specific antigen. Current knowledge about MAGED4 expression in glioma is only based on mRNA analysis and MAGED4 protein expression has not been elucidated. In the present study, we investigated this point and found that MAGED4 mRNA and protein were absent or very lowly expressed in various normal tissues and glioma cell line SHG44, but overexpressed in glioma cell lines A172,U251,U87-MG as well as glioma tissues, with significant heterogeneity. Furthermore, MAGED4 protein expression was positively correlated with the glioma type and grade. We also found that the expression of MAGED4 inversely correlated with the overall methylation status of the MAGED4 promoter CpG island. Furthermore, when SHG44 and A172 with higher methylation were treated with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZA-CdR) reactivation of MAGED4 mRNA was mediated by significant demethylation in SHG44 instead of A172. However, 5-AZA-CdR treatment had no effect on MAGED4 protein in both SHG44 and A172 cells. In conclusion, MAGED4 is frequently and highly expressed in glioma and is partly regulated by DNA methylation. The results suggest that MAGED4 might be a promising target for glioma immunotherapy combined with 5-AZA-CdR to enhance its expression and eliminate intratumor heterogeneity.
Two Androstane Derivatives from the Cultures of Fungus Marasmiellus ramealis (Bull.) Singer
Yang, Ning-Ning,Ma, Qing-Yun,Huang, Sheng-Zhuo,Dai, Hao-Fu,Guo, Zhi-Kai,Yu, Zhi-Fang,Zhao, You-Xing Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.11
A new androstane derivative, $4{\beta}$-methyl-15-oxa-$14{\beta}$-androstane-7-ene-$4{\alpha}$-carboxylic acid (1) and a known one $4{\beta}$-methyl-15-oxa-$14{\beta}$-androstane-7-ene-$4{\alpha}$-hydroxyl (2) were isolated from the EtOAc extract of the cultures of the fungus Marasmiellus ramealis (Bull.) Singer. Their structures were elucidated on the basis of 1D and 2D NMR as well as MS spectroscopic data analysis. The inhibitory activity of two isolates against acetylcholinesterase (AChE) revealed that compound 1 exhibited definitely inhibitory activity.
서비스디자인에 기초한 외국 유학생의 대학 생활 실태 사례 연구 -"K"대학을 중심으로-
신계옥(주저자) ( Qing Zhou ),주경(공동저자) ( Qing Zhou ),주우(공동저자) ( Yu Zhou ),녕미형 ( Mei Ying Ning ),무문일 ( Wen Yi Wu ),서신희 ( Chen Xi Xu ) 커뮤니케이션디자인학회 2015 커뮤니케이션 디자인학연구 Vol.52 No.-
본 연구는 서비스 디자인 관점에서 ``K``대학에서 공부하고 있는 외국인 34명의 유학생을 대상으로 서비스디자인의 사고방식과 도구 활용 및 프로세스와 방법을 적용하여 대학 생활에서의 불편 요인을 파악하고 문제점을 도출하고자 하였다. 그 결과 대학 생활에서의 유형, 무형의 불편 요인의 실체를 파악할 수 있었다. 즉, 식당 및 기숙사, 강의실에서의 문제들과 교내외 교통 및 이동 수단, 외국인들을 위한 안내책자의 부재로 인한 대학생활의 불편한 사항들이 파악되었다. 이러한 문제들이 개선되고 해결되어야 ``K`` 대학에서 일정기간동안 유학하는 외국 유학생들이 불편한 점이 없이 교육적 목적을 달성할 수 있으며, 이후에도 미래의 친한인재로 교류의 기틀이 되어 바람직한 미래의 가치가 창출될 수 있다고 생각한다. This paper, from the point of view of the design of services, take advantage of the service design concepts and tools to target foreigners 34 international students who are studying at the University of "K", to apply the process and methods of college life grasps the inconvenience factors in the medium, it is attempted to derive a problem. As a result, it was able to grasp the tangible, intangible of failure factor entities in college life. In other words, transportation and means of transportation outside the school, inconveniences of college life by the absence of a brochure for foreigners and restaurant, dormitory, is a problem in the classroom has been grasped. Basis of these issues are improved at the University of "K" should be resolved foreign students is inconvenient point without having a certain period of time studying abroad, it is possible to achieve the educational purposes, after also exchanges in the future of the close human resources I think that the value of the desired future is created to become.
SVM-Based Incremental Learning Algorithm for Large-Scale Data Stream in Cloud Computing
( Ning Wang ),( Yang Yang ),( Liyuan Feng ),( Zhenqiang Mi ),( Kun Meng ),( Qing Ji ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.10
We have witnessed the rapid development of information technology in recent years. One of the key phenomena is the fast, near-exponential increase of data. Consequently, most of the traditional data classification methods fail to meet the dynamic and real-time demands of today`s data processing and analyzing needs--especially for continuous data streams. This paper proposes an improved incremental learning algorithm for a large-scale data stream, which is based on SVM (Support Vector Machine) and is named DS-IILS. The DS-IILS takes the load condition of the entire system and the node performance into consideration to improve efficiency. The threshold of the distance to the optimal separating hyperplane is given in the DS-IILS algorithm. The samples of the history sample set and the incremental sample set that are within the scope of the threshold are all reserved. These reserved samples are treated as the training sample set. To design a more accurate classifier, the effects of the data volumes of the history sample set and the incremental sample set are handled by weighted processing. Finally, the algorithm is implemented in a cloud computing system and is applied to study user behaviors. The results of the experiment are provided and compared with other incremental learning algorithms. The results show that the DS-IILS can improve training efficiency and guarantee relatively high classification accuracy at the same time, which is consistent with the theoretical analysis.
Ning Wang,Qing Wang,Shuangshuang Xu,Lulu Lei 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-
Concrete with superhydrophobicity can protect it from being eroded by water and reduce accumulationof dust. Herein, a green and efficient method was proposed for fabricating hierarchical structures on concretesurfaces with superhydrophobicity by directly replicating micro-nano dendritic structures fromsuperhydrophobic copper mesh to fresh concrete. Microstructures, chemical compositions and wettabilityof the as-fabricated concrete were characterized. Superhydrophobicity of the as-fabricated concretewas studied. Effect of water jet on the as-fabricated concrete was investigated. Wetting durability ofthe as-fabricated concrete were studied. Self-cleaning property was confirmed. It was found that owingto the successful replication of micro-nano dendritic structures and low surface energy stearic acid, theas-fabricated concrete displayed good superhydrophobicity, wetting durability and self-cleaningproperty.
Analytical solution for natural frequency of monopile supported wind turbine towers
Xue-Ning Rong,Ri-Qing Xu,Heng-Yu Wang,Su-Yang Feng 한국풍공학회 2017 Wind and Structures, An International Journal (WAS Vol.25 No.5
In this study an analytical expression is derived for the natural frequency of the wind turbine towers supported on flexible foundation. The derivation is based on a Euler-Bernoulli beam model where the foundation is represented by a stiffness matrix. Previously the natural frequency of such a model is obtained from numerical or empirical method. The new expression is based on pure physical parameters and thus can be used for a quick assessment of the natural frequencies of both the real turbines and the small-scale models. Furthermore, a relationship between the diagonal and non-diagonal element in the stiffness matrix is introduced, so that the foundation stiffness can be obtained from either the p-y analysis or the loading test. The results of the proposed expression are compared with the measured frequencies of six real or model turbines reported in the literature. The comparison shows that the proposed analytical expression predicts the natural frequency with reasonable accuracy. For two of the model turbines, some errors were observed which might be attributed to the difference between the dynamic and static modulus of saturated soils. The proposed analytical solution is quite simple to use, and it is shown to be more reasonable than the analytical and the empirical formulas available in the literature.
Production of Recombinant Humanized Anti-HBsAg Fab Fragment from Pichia pastoris by Fermentation
Deng, Ning,Xiang, Junjian,Zhang, Qing,Xiong, Sheng,Chen, Wenyin,Rao, Guirong,Wang, Xunzhang Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
In this report, we describe the high-yield secretory expression of the recombinant human anti-HBsAg Fab fragment from Pichia pastoris that was achieved by co-integration of the genes encoding the heavy and light chains (both under the control of alcohol oxidase promoter) into the genome of the yeast cells. The fed-batch fermentations were carried out in a 5 L scale. Both chains of the Fab were successfully expressed upon methanol induction. The absorbance ($OD_{600}$) of the broth can reach 350~500 at the end of fed-batch phase. After the induction, the expression level of the recombinant Fab (soluble) reached 420~458 mg/L. The recombinant Fab fragment was purified from the crude culture supernatant by ion exchange chromatography and the purity of the recombinant Fab fragment was over 95%. The affinity activities of the crude fermentation supernatant and the purified Fab were analyzed by indirect ELISA, which showed that the purified recombinant Fab fragment had high affinity activity with hepatitis B surface antigen.
Lan-Qing Ma,Yan-Wu Guo,Hui-Li Guo,Xing Li,Li-Li Huang,Bo-Ning Zhang,Xiao-Bin Pang,Ben-Ye Liu,Hong Wang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
In our recent work (Ma et al., in Planta229(3):457–469, 2009a and 229(4):1077–1086, 2009b),two three-intron type III PKS genes, PcPKS1 and PcPKS2,were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is athree-intron chalcone synthase (CHS) gene, and PcPKS2 isfound to be a three-intron benzalacetone synthase (BAS)gene. The regular CHS encoded by a single intron genehave not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3)and a stilbene synthase (STS) gene with three-intron(PcPKS5) were isolated and characterized by functionaland phylogenetic analyses. In comparison with PcPKS1, abifunctional enzyme with both CHS and BAS activity, theenzymatic product of recombinant PcPKS3 was naringenin,bis-noryangonin (BNY) and 4-coumaroyltriacetic acidlactone (CTAL) occurred as side products. The PcPKS5synthesized resveratrol and a trace amount of naringeninfrom p-coumaroyl-CoA. To our knowledge, PcPKS5 is thefirst reported three-intron STS gene in flowering plants. Inthis work, we speculated that this involved a possibleevolutionary route of plant-specific type III PKS superfamilyin P. cuspidatum.