담낭절제술 후 발현된 간, 신장 및 복막을 침범한 유전분증 1예

김성연,김덕영,김경아,송준호,이승우,한지영,김문재 대한내과학회 2003 대한내과학회지 Vol.65 No.1

저자 등은 최근 담낭절제술을 시행받은 후에 발생된 간기능 이상과 조절되지 않는 복수를 주소로 내원한 환자에서 진단된 유전분증 1예를 경험하였기에 보고하는 바이다. Amyloidosis is a heterogenous group of often fatal disorders characterized by extracellular deposition of a proteinaceous material with a unique fibrillar form in various tissues and organs. Deposition of amyloid may cause widespread dysfunction of the involved organs. Recently, we experienced a case of 40-year-old male patient with nephrotic syndrome, uncontrolled ascites and jaundice, which developed after cholecystectomy. We confirmed systemic amyloidosis involving kidney, liver and peritoneum by kidney and liver and peritoneum biopsy. The case that, having about a month of incubation period after cholecystectomy, amyloidosis occurred in a healthy man before the operation, and that amyloid was deposited in the peritoneum site incised during the operation provides grounds that amyloidosis may be triggered by cholecystectomy. Given the rarity of this case, the following is a report of this case and a review of the relevant literatures.

Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces

Moon, Yeon-Hee,Yoon, Mi-Kyeong,Moon, Jung-Sun,Kang, Jee-Hae,Kim, Sun-Hun,Yang, Hong-Seo,Kim, Min-Seok The Korean Academy of Prosthodonitics 2013 The Journal of Advanced Prosthodontics Vol.5 No.3

PURPOSE. To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces. MATERIALS AND METHODS. Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot. RESULTS. There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface. CONCLUSION. These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.

Effects of CoCl2 on Osteogenic Differentiation of Human Mesenchymal Stem Cells

Yeon-Hee Moon,Jung-Wan Son,Jung-Sun Moon,Jee-Hae Kang,Sun-Hun Kim,Min-Seok Kim The Korean Academy of Oral Biology 2013 International Journal of Oral Biology Vol.38 No.3

Objective. To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation–mimicking agent cobalt chloride (CoCl2) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying molecular mechanisms. Study design. The dose and exposure periods for CoCl2 in hMSCs were optimized by cell viability assays. After confirmation of CoCl2-induced HIF-1α and vascular endothelial growth factor expression in these cells by RT-PCR, the effects of temporary preconditioning with CoCl2 on hMSC osteogenic differentiation were evaluated by RTPCR analysis of osteogenic gene expression, an alkaline phosphatase (ALP) activity assay and by alizarin red S staining. Results. Variable CoCl2 dosages (up to 500 μM) and exposure times (up to 7 days) on hMSC had little effect on hMSC survival. After CoCl2 treatment of hMSCs at 100 μM for 24 or 48 hours, followed by culture in osteogenic differentiating media, several osteogenic markers such as Runx-2, osteocalcin and osteopontin, bone sialoprotein mRNA expression level were found to be up-regulated. Moreover, ALP activity was increased in these treated cells in which an accelerated osteogenic capacity was also verified by alizarin red S staining. Conclusions. The osteogenic differentiation potential of hMSCs could be preserved and even enhanced by CoCl2 treatment.

토석류 표면유속 측정을 위한 딥러닝을 이용한 비접촉식 STIV 기법의 적용

김연중(Kim, Yeon Joong),김태우(Kim, Tea Woo),윤종성(Yoon, Jong Sung),정재훈(Jeong, Jae Hoon) 대한토목학회 2020 대한토목학회 학술대회 Vol.2020 No.10

Outbreak of Nosocomial Urinary Tract Infections caused by Multidrug-Resistant Pseudomonas aeruginosa

Park, Yeon-Joon,Oh, Eun-Jee,Kim, Gi-Gum,Kim, So-Yeon,Kim, Sang-Taek,Yoo, So-Yeon,Kim, Yang-Ree,Kang, Moon-Won,Kim, Byung-Kee 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-

Background : Nosocomial urinary tract infection (UTI) accounts for 35% of the nosocomial infection and 80-90% of them are associated with urethral catheters. Recently, we experienced an outbreak of nosocomial UTI caused by multidrug-resistant Pseudomonas aeruginosa in neurosurgical intensive care unit(NSICU). Methods : We investigated clinical records of the patients and observed the methods of care of urethral catheters in NSICU. Identification of P. aeruginosa was done by disk diffusion method. Random Amplification of Polymorphic DNA (RAPD) assay was used as a genotyping method. Results : Between November 1997 and January 1998, eleven P. aeruginosa stains were isolated from the urine of 11 patients hospitalized in NSICU of Kangnam St. Mary's Hospital. Routine regular bladder irrigation, and emptying urine with common urinal had been done falsely. Antibiogram of the isolates showed clonal relatedness, which was different from those of other clinical strains. We instructed all the health care workers to stop bladder irrigation, and to use separate urinals for each patient. Thereafter, no further case of P. aeruginosa UTI has occurred. Conclusion : An outbreak of UTI, caused by a single clone of P. aeruginosa, was confirmed by RAPD and was eradicated after correchion of false practice on care of urinary catheter. (Korean Journal of Nosocomial Infection control 4(1):1-6, 1999)

미얀마에서 유입된 뎅기쇽 증후군(Dengue Shock Syndrome) 1례

문성용,노영준,김종혁,김지연,김진건,김현우,이정훈,남상민 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

저자들은 미얀마에서 현지 상사원 부인으로 2년간 거주 중 발병한 뎅기쇽 증후군 1예를 경험하였다. 환자는 내원 6일전부터 발열, 오한, 전신쇠약, 근육통, 오심 등의 증상이 있었으나 치료를 지연하다가 귀국하였으며 환자는 내원당시 점상 출혈 및 쇼크를 보였고 적절한 수액요법 등을 시행하였으나 내원 2일째 사망하였다. 환자는 국립보건원에 의뢰한 뎅기바이러스에 대한 IgM antibody capture ELISA 검사에서 양성반응을 보여 뎅기쇽 증후군으로 확진되었다. Dengue fever/dengue shock syndrome is an acute febrile illness caused by a Flaviviridae virus. Dengue virus infection can cause a wide spectrum of illness, and disease is characterized by sudden onset of high fever, chill, severe headache, skin rash, general malaise and thrombocytopenia with hemoconcentration. Dengue fever is endemic in most tropical areas of world, including the Caribbean, Central and South America, Africa, and Southeast Asia. Tourists to these areas are liable to infection. We experienced a Korean female who returned to Korea from Myanmar in severely ill state. She was confirmed serologically to be inblieted with Dengue shock syndrome. In spite of intensive medical care, she died of Dengue shock syndrome. We emphasize that favorable prognosis depends mainly on the early recognition and treatment of shock.

GG-7 : Recurrence after pelvic organ prolapse surgery in normal-weight compared with overweight and obese women

( Jee Whan Kim ),( Su Yeon Park ),( Yeo Jung Moon ),( Sei Kwang Kim ),( Sang Wook Bai ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: To compare recurrent prolapse after vaginal pelvic organ prolapse surgery in normal-weight compared with overweight and obese women. 방법: We performed a retrospective cohort study of the risk of recurrent prolapse after vaginal pelvic organ prolapse surgery (including vaginal hysterectomy, anterior vaginal colporrhaphy, posterior vaginal colporrhaphy or iliococcygeus fixation) in normal-weight compared with overweight and obese women at our institution from March 2011, through December 2012. We compared 4 groups: normal weight (BMI 18.5-22.9) compared with overweight (BMI 23.0-24.9), obese 1 (BMI 25.0-29.9) and obese 2 (BMI 30.0 or greater). Women with fewer than 6 months of follow-up and those with a prior prolapse surgery were excluded. Our primary outcome was a composite measure defined as anterior, posterior, or apical prolapse recurrence extending beyond the hymen or repeat treatment for symptomatic prolapse with surgery. 결과: We included 238 participants (82 [34%] normal-weight, 60 [25%] overweight, 88 [37%] obese 1 group and 8 [3%] obese 2 group women). There was no difference in age, vaginal parity, menopause, types of concomitant surgery, pre-operative POP quantification scores between 4 groups. And there was no difference in mean follow-up, 19.4±20.2 months in the normal weight compared with 21.5±18.0 in the overweight, 28.7±26.6 in the obese 1 and 17.8±24.6 in the obese 2 group (p=0.243). There was significant difference in recurrence; Prolapse recurred in 3.7% (n=3) of the normal-weight group, 1.7% (n=1) of the overweight group, 5.7% (n=5) of the obese 1 group and 25.0% (n=2) of the obese 2 group (p=0.028). Surgery for recurrent prolapse was performed in 5 women with symptomatic prolapse (p=0.737) and the most common site of recurrence was the anterior compartment [n=9] compared with the apical compartment [n=2] or posterior compartment [n=0] with no significant difference between 4 groups (p=0.132). 결론: After vaginal pelvic organ prolapse surgery, overweight or obese women have higher prolapse recurrence compared with normal-weight women. Anterior prolapse is the most common site of recurrence after vaginal pelvic organ prolapse surgery.

Laparoscopy-assisted distal gastrectomy for gastric cancer after liver transplantation

Moon-Soo Lee,Eun-Young Kim,Ju-Hee Lee,Ye Seob Jee,Do Joong Park,김형호,So Yeon Kim,최유신 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.80 No.6

A case report described a 72-year-old man with a history of a deceased-donor liver transplantation (due to hepatitis B-associated end-stage liver cirrhosis) performed in 1994. The patient was diagnosed with renal cell carcinoma and pulmonary metastasis in 1997 and was successfully treated with radiofrequency ablation and thoracoscopic superior segmentectomy. There was no evidence of newly diagnosed metastatic lesions or recurrence until the 19th post-operative month. Gastric cancer was identified by endoscopy during a routine follow-up examination; the pre-pyloric antral lesion measured 1.5 cm in size and was histologically well-differentiated and confined to the submucosal layers on endoscopic ultrasound. Laparoscopic gastrectomy and lymph node dissection (D1 + b) was successfully performed in March 2009, and the patient was discharged on the 5th post-operative day without complications. This suggests that laparoscopic surgery is one of the feasible methods for resection of gastric cancer in liver transplant patients.

인체 재대혈 조혈모세포의 체외 증식

문한림,송호철,김훈교,한지연,김춘추,이경식,김동집,김태응 한국BRM학회 1995 한국 BRM학회지 Vol.5 No.2

Evaluation Method on Destruction and Removal Efficiency of Perfluorocompounds from Semiconductor and Display Manufacturing

Jee-Yon Lee,Jin Bok Lee,Dong Min Moon,Jun Hyung Souk,Seung Yeon Lee,Jin Seog Kim* 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.8

Recently, the semiconductor and display industries have tried to reduce the emissions of perfluorocompounds (PFCs) from the globally environmental regulation. Total amount of PFC emission can be calculated from the flow rate and the partial pressures of PFCs. For the precise measurement of PFC emission amount, the mass flow controlled helium gas was continuously injected into the equipment of which scrubber efficiency is being measured. The partial pressures of PFCs and helium were accurately measured using a mass spectrometer in each sample extracted from inlet and outlet of the scrubber system. The flow rates are calculated from the partial pressures of helium and also, PFC destruction and removal efficiency (DRE) of the scrubber is calculated from the partial pressure of PFC and the flow rate. Under this method, the relative expanded uncertainties of the flow rate and the partial pressures of PFCs are ± 2% (k = 2) in case the concentrations of NF3 and SF6 are as low as 100mmol/mol.