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Identification of molecular markers for the oncogenicdifferentiation of hepatocellular carcinoma
Gyung-Ran Yu,Seong-Hun Kim,Seon-Hwa Park,Xiang-Dan Cui,Dong-Yuan Xu,Hee-Chul Yu,Baik-Hwan Cho,Young-Il Yeom,Sang-Soo Kim,Sang-Bae Kim,추인선,김대곤 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.5
The aim of this study was to identify molecular markers associated with oncogenic differentiation in hep-atocellular carcinoma (HCC). Using an unsupervised clustering method with a cDNA microarray, HCC (T) gene expression profiles and corresponding non-tu-tal 217 genes, 72 were expressed preferentialy in HCC tissues. Among 186 diferentialy regulated genes, there were molecular chaperone and tumor sup-pressor gene clusters in the Edmondson grades I and I (GI/I) subclass compared with the liver cirrhosis (LC) subclass. The Edmondson grades III and IV (GIII/IV) subclass with a poor survival (P = 0.0133) contained 122 diferentialy regulated genes with a cluster containing pared with the GI/II subclass. Imunohistochemical analysis revealed that ANXA2, one of the 72 genes pref-erentially expressed in HCC, was over-expressed in the sinusoidal endothelium and in malignant hepatocytes in HCC. The genes identified in the HCC subclasses wil be useful molecular markers for the genesis and pro-gresion of HCC. In addition, ANXA2 might be a novel marker for tumor angiogenesis in HCC.
( Mi Jin Lee ),( Gyung Ran Yu ),( Seon Hwa Park ),( Baik Hwan Cho ),( Jong Seong Ahn ),( Hae Joon Park ),( Eun Young Song ),( Dae Ghon Kim ) 대한소화기학회 2007 SIDDS Vol.9 No.-
The poor survival rate of a hepatocellular carcinoma (HCC) is in part attributed to the inability to diagnose patients at an early stage. Therefore, the aim of this study was to search for candidate serum marker for HCC and to test their ability to distinguish a HCC from benign liver disease. Methods: Genome-wide analysis by a microarray in 40 HCC patients was performed between HCC and paired nontumor liver tissues. Expression of cystatin B (CSTB) was examined by mRNA expression analysis and immuno-histochemistry. The serum CSTB levels were measured using a sandwich ELISA method in four groups including normal healthy subjects (G1, n=52), patients with non-cirrhotic chronic hepatitis (G2, n=53), patients with cirrhosis (G3, n=43), and patients with HCC (G4, n=62). Results: Unsupervised hierarchical clustering analysis identified 248 genes that were expressed differently between HCC and nontumor liver tissues. One of them, CSTB, was expressed preferentially in the HCCs compared with the nontumor tissues, 36 out of 45 specimens (80%) by Northern blot and semi-quantitative reverse transcriptase-PCR(RT-PCR) analyses. The serum CSTB level was much higher in HCC patients than in those with nonmalignant chronic liver disease (G2 and G3) (P<0.0001). The receiver operating characteristic (ROC) curve indicated 5.34 ng/ml to be the optimal value for CSTB, and the sensitivity and specificity for this CSTB value was 85.5% (95% confidence interval, 74.2%-93.1%) and 53.1% (95% CI, 42.7%-63.4%), respectively, in distinguishing between patients with HCC and those with non malignant chronic liver disease. Conclusions: CSTB is specifically overexpressed in most HCCs and is also elevated in the serum of a large proportion of HCC patients. CSTB or the combination of CSTB and α-fetoprotein (AFP) may be a useful marker for diagnosing patients with HCC with a high sensitivity.
인삼 잿빛곰팡이병균 Botrytis cinerea의 균학적 특성
조혜선(Hye Sun Cho),전용호(Yong Ho Jeon),도경란(Gyung-Ran Do),조대휘(Dae-Hui Cho),유연현(Yun-Hyun Yu) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1
인삼 고년생 결주의 주원인으로 작용하는 잿빛곰팡이병 방제을 위한 기초 연구로 병원균을 분리, 동정하고 이들의 균학적 특성을 구명하였다. 잿빛곰팡이병반으로부터 균을 분리하여 분생포자의 형태적 특징을 관찰하고 RPB2 유전자의 염기서열 분석을 실시한 결과 분리균은 Botrytis cinerea로 동정되었다. 균핵을 이용한 병원성 검정 결과 공시한 3균주 중 1균주는 병원성, 2 균주는 비병원성을 나타내었다. 주사전자현미경으로 균핵을 관찰한 결과 병원성 균주는 표면이 주름지고 거친 반면 비병원성 균주는 매끈하였다. 균핵 형성은 5~20℃, 암 상태에서 양호하였으며, 0.5% 고죽을 첨가한 배지에서 10배 정도 증가하였다. A total of 29 Botrytis were isolated from ginseng gray mold at 8 locations in Korea from June to July, 2004 and 2005. The causative agent of them was identified as Botrytis cinerea based on morphological characteristics of conidia and RPB2 gene sequence analysis. In inoculation experiments with sclerotia, one isolate was pathogenic to ginseng plant whereas two isolates were non-pathogenic. Scanning electron microscopy revealed that the sclerotia of pathogenic isolate has rough surface and that of non-pathogenic isolate very smooth surface. Optimum temperature and culture conditions for sclerotia production were 5~20℃ and darkness, respectively. The number of sclerotia was increased tenfold on media added with 0.5% old stem fragment of ginseng.