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인삼 잘록병(Rhizoctonia solani) 발생억제에 미치는 Fludioxonil, Flutolanil 및 Thifluzamide의 효과
조대휘(Dae-Hui Cho),유연현(Yun-Hyun Yu) 고려인삼학회 2005 Journal of Ginseng Research Vol.29 No.4
In vitro and in vivo effectiveness of fungicides were evaluated for the control of damping off caused by Rhizoctonia solani on Panax ginseng. Fludioxonil(67 ㎎ a. i./L), flutolanil(75 ㎎ a. i./L), thifluzamide(35 ㎎ a. i./L), and mepronil (750 ㎎ a. i./L) were selected from 9 fungicides, which were based on inhibition of mycelial growth of R. solani (isolate Rh 9801) and duration of fungicidal effectiveness against the pathogen in vitro. Field trials were made twice in the year of 2003 and 2004. Experimental plots (54 m×0.9 m) of 4-year-old ginseng fields were artificially infested with 5㎏ and 14 ㎏ in fresh weight of inoculum in 2003 and 2004, respectively. The fungicides were drenched at a volume of 8l in 3.6 m×0.9 m with 3 replications. Fludioxonil, flutolanil, thifluzamide and mepronil reduced the incidence of damping off by 73%, 69%, 69% and 43%, respectively. In the 2004 trial, fludioxonil, flutolanil, and thifluzamide showed similar result as reducing the incidence by 85%, 84%, and 82%, respectively, in the plot where the inoculum was applied 2.8 times more than the 2003. The disease incidences in untreated control were 12% in 2003 and 47% in 2004.
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김기황,정훈채,김용연,이미경,유연현,Kim Ki-Whang,Jeong Hun-Chae,Kim Yong-Yeon,Lee Mee-Kyoung,Yu Yun-Hyun 한국연초학회 2005 한국연초학회지 Vol.27 No.1
Studies on effect of Decyl alcohol EC and Butralin EC on tobacco sucker control were conducted in the tobacco field of Agro-tech Research Group of KT&G Central Research Institute, in 2004. Control effects of two chemicals were highest at the sucker length of 1$1\~2cm$. This indicates that the first application of Decyl alcohol should be conducted before button stage in the flue-cured and at the button stage in the burley tobacco. Effects of Decyl alcohol and Butralin on sucker control were $89.6\%\;and\;90.7\%$, respectively at 14 days after application in the variety KF118. But that of sequential application of two chemicals was $81.9\%$ at 30 days after application in the variety KF118 and $86.1\%$ in the variety KB303. Effect of two applications of Yellow ribbon or Butralin was highest in two varieties. The central downward spray showed a little higher control effect than the pouring of two chemicals. There was not significant difference of sucker control effect between forty and fifty times solution of Decyl alcohol in the variety KB303.
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인삼 뿌리썩음병균 Cylindrocarpon destructans 후막포자의 형태적 특성
조대휘(Dae-Hui Cho),유연현(Yun-Hyun Yu),김영호(Young Ho Kim) 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.4
Chlamydospore formation from mycelia and conidia of Cylindrocarpon destructans isolated from the root rot lesion of the Panax ginseng was investigated by scanning electron and light microscopy. Typical chlamydospores were formed only from hyphae but not from conidia on culture media. However, immature chlamydopspore-like cells were<br/> formed from microconidia after 12 days of incubation at 20℃ on Czapek Dox broth (CDB) adjusted to pH 4.0. Chlamydospores were yellowish or reddish brown in color, and produced singly or in chain with the hyphal intercalary or terminal position on potato-dextrose agar, V-8 juice agar and CDB with no addition of nitrogen sources after 16~20 days of incubation at 20℃. They were 11.3 to 11.9 ㎛ in diameter, having many lumps-like warts on their surface with the length of 1.5 to 1.8 ㎛.
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인삼 잘록병균 Rhizoctonia solani의 균사융합군과 병발생 및 생육 특성
조대휘(Dae-Hui Cho),강제용(Je Yong Kang),유연현(Yun-Hyun Yu) 고려인삼학회 2004 Journal of Ginseng Research Vol.28 No.4
On May of 2002, the 34 isolates of Rhizoctonia solani were isolated from the symptom of damping-off on basal stems of 2-year-old to 6-year-old Panax ginseng which were cultivated in the 17 fields in Kyunggi-do, Chungcheungnam-do and Jeollabuk-do province in Korea. All isolates were identified as anastomosis group 2-1. Pre-emergence damping-off occurred on underground part of stem of 2-year-old ginseng in the pot trial with artificial inoculation. However, in the 4-year-old ginseng field with artificial inoculation, post-emergence damping-off occurred. The severe incidence of damping-off was found in the 6-year-old ginseng field in Kimje-si, Jeollabuk-do province on June 5 of2003, the rate of which showed 18.6% of area in the field by spread of the disease since 2-year-old. The sclerotia of R. solani, started to be formed after 7 days incubation on potato dextrose agar at 25℃, were grayish brown, spherical to irregular and about 500 ㎛ in diameter, which became dark brown after 14 days incubation. The temperature range for the mycelial growth of R. so/ani isolates was 5~30℃, and the optimal temperature was 25℃, their growth were very poor at 5 or 30℃. The isolates grew at the range of pH 4.5~8.1 tested and optimal pH for growth was pH 4.5~5.8, whereas their growth were very poor above the pH 7.2.
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인삼 잿빛곰팡이병균 Botrytis cinerea의 균학적 특성
조혜선(Hye Sun Cho),전용호(Yong Ho Jeon),도경란(Gyung-Ran Do),조대휘(Dae-Hui Cho),유연현(Yun-Hyun Yu) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1
인삼 고년생 결주의 주원인으로 작용하는 잿빛곰팡이병 방제을 위한 기초 연구로 병원균을 분리, 동정하고 이들의 균학적 특성을 구명하였다. 잿빛곰팡이병반으로부터 균을 분리하여 분생포자의 형태적 특징을 관찰하고 RPB2 유전자의 염기서열 분석을 실시한 결과 분리균은 Botrytis cinerea로 동정되었다. 균핵을 이용한 병원성 검정 결과 공시한 3균주 중 1균주는 병원성, 2 균주는 비병원성을 나타내었다. 주사전자현미경으로 균핵을 관찰한 결과 병원성 균주는 표면이 주름지고 거친 반면 비병원성 균주는 매끈하였다. 균핵 형성은 5~20℃, 암 상태에서 양호하였으며, 0.5% 고죽을 첨가한 배지에서 10배 정도 증가하였다. A total of 29 Botrytis were isolated from ginseng gray mold at 8 locations in Korea from June to July, 2004 and 2005. The causative agent of them was identified as Botrytis cinerea based on morphological characteristics of conidia and RPB2 gene sequence analysis. In inoculation experiments with sclerotia, one isolate was pathogenic to ginseng plant whereas two isolates were non-pathogenic. Scanning electron microscopy revealed that the sclerotia of pathogenic isolate has rough surface and that of non-pathogenic isolate very smooth surface. Optimum temperature and culture conditions for sclerotia production were 5~20℃ and darkness, respectively. The number of sclerotia was increased tenfold on media added with 0.5% old stem fragment of ginseng.
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인삼 배발생세포에서 재분화된 유식물체의 뿌리 발육에 미치는 Phytagel의 영향
안인옥(In-Ok Ahn),최영준(Young-Jun Choi),이성식(Sung-Sik Lee),이장호(Jang-Ho Lee),강제용(Jae-Yong Kang),유연현(Yun-Hyun Yu),정희돈(Hee-Don Chung) 고려인삼학회 2004 Journal of Ginseng Research Vol.28 No.4
This study was carried out to find out the optimum hardening condition for ginseng plantlets redifferentiated by tissue culture method. While a lot of root hair were observed on the root of seedling grown on the soil, few root hair were observed on the root of plantlet redifferentiated in vitro. On the medium solidified with 0.1 % phytagel, root hair was not observed and root weight, root width and laternal root development were also very poor. While raising the phytagel concentration on the media, root hair began to increase and root weight, root width and laternal root development were improved. Vascular tissue of plantlet grown on the medium with 0.1 % phytagel was very poor, but that of plantlet grown on the medium with 0.8% phytagel was very good.
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