Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema Lectin and chemical modification properties on its mannose-binding sites

( Bo Liu ),( Xiao Chao Xu ),( Yan Cheng ),( Jian Huang ),( Yan Hong Liu ),( Zhen Liu ),( Ming Wei Min ),( He Jiao Bian ),( Jing Chen ),( Jin Ku Bao ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.5

On-Chip Touch Sensor Readout Circuit Using Passive Sigma-Delta Modulator Capacitance-to-Digital Converter

Bo Liu,Hoseini, Zaniar,Kye-Shin Lee,Yong-Min Lee IEEE 2015 IEEE Sensors Journal Vol. No.

<P>This paper describes a capacitive type touch sensor readout circuit using passive sigma-delta modulator-based capacitance-to-digital converter. With the proposed approach, the panel condition (touched or untouched) can be effectively converted into the digital level using the panel capacitance as the summing element of the first-order passive sigma-delta modulator. In addition, the proposed touch sensor readout circuit does not require an analog-to-digital converter, since the digital format corresponding to the touch panel condition can be simply obtained by counting the number of modulator output pulses within the detection period. This along with the passive architecture leads to a compact and low-power on-chip touch sensor readout circuitry. Furthermore, due to the noise shaping property of the sigma-delta modulator, a comparable SNR and detection time with respect to other touch sensor readout circuits can be achieved. The proposed touch sensor readout circuit is implemented using CMOS 0.35-μm technology with core area of 0.1 mm<SUP>2</SUP>. The operation of the sensor readout circuit is verified with a 10.4' projective capacitance type touch panel, where the measured SNR is 31.4 dB with power consumption of 65 μW.</P>

Molecular Cloning and Bioinformatic Analysis of SPATA4 Gene

Liu, Shang-Feng,Ai, Chao,Ge, Zhong-Qi,Liu, Hai-Luo,Liu, Bo-Wen,He, Shan,Wang, Zhao Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6

Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.

Discrete Particle Swarm Optimization Algorithm in Flexible Hybrid Flow Shop Scheduling

Liu Dongdong,Liu Kai,Zhao Zhengping Han Bo,Zhang Yan 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.10

Traditional flexible flow shop scheduling cannot adapt to the work processes with existence of parallel machines, and blocks or limits the processes with no-wait constraints. Firstly, according to the problem in NWBFFSSP, which minimizes the maximum time used in the flow shop, an optimal solving model has been designed to realize the flexible flow shop scheduling with multi constraints; besides, for the distribution of machinery is improved, Finally, in the solving process, a real-time release priority strategy has been proposed to determine processing machine for each work piece. Furthermore, a methodology to detect work piece conflicts has been introduced while the conflicts are then eliminated by a kind of right moving strategy based on the maximum difference. The experimental results verify the effectiveness and feasibility of the proposed algorithm.

Influence of pore structure of granular activated carbon prepared from anthracite on the adsorption of CO2, CH4 and N2

Bo Zhang,Zhuoran Huang,Ping Liu,Jin Liu,Min Gu 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.3

A series of granular activated carbon (GAC) samples with similar surface chemical properties but different pore structures were prepared from anthracite. The maximum adsorption capacities of the prepared CO2, CH4, and N2 at 298 K and 2.0MPa were 4.27mmol/g, 2.54mmol/g, and 1.46mmol/g, respectively, and the adsorption selectivity parameters, i.e., CH4,N2 and CO2,CH4, were 3.23 and 3.06, respectively. By using the GAC with the optimum pore size as adsorbent, the concentration of methane in the nitrogen-methane (CH4/N2) mixture was concentrated from 30% to 63.5% via a single-column single-cycle pressure swing adsorption (PSA) process. The pore size distribution of the GAC samples was dominated by micropores, with specific surface area in the range of 330-500m2/g and micropore volume in the range of 0.12-0.19 cm3/g. Although the specific surface area and pore volume of micropores played an important role in the separation performance, the pore size distribution was found to be the decisive factor. In particular, the micropores with sizes in the range of 5.0-10.0 Å were the main factor affecting the concentrating effect of CH4 or CO2 by GAC.

Diagnostic Value of ¹⁸F-FDG PET/CT in Comparison to Bone Scintigraphy, CT and ¹⁸F-FDG PET for the Detection of Bone Metastasis

Liu, Ning-Bo,Zhu, Lei,Li, Ming-Huan,Sun, Xiao-Rong,Hu, Man,Huo, Zong-Wei,Xu, Wen-Gui,Yu, Jin-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Purpose: To evaluate the diagnostic value of $^{18}F$-FDG PET/CT for detection of bone metastasis in comparison with the efficacies of $^{18}F$-FDG PET/CT, CT, $^{18}F$-FDG PET and conventional planar bone scintigraphy in a series of cancer patients. Methods: Five hundred and thirty patients who underwent both $^{18}F$-FDG PET/CT and bone scintigraphy within 1 month were retrospectively analyzed. The skeletal system was classified into 10 anatomic segments and interpreted blindly and separately. For each modality, the sensitivity, specificity, accuracy, PPV and NPV were calculated and the results were statistically analyzed. Results: Bone metastases were confirmed in 117 patients with 459 positive segments. On patient-based analysis, the sensitivity, specificity, accuracy, PPV and NPV of $^{18}F$-FDG PET/CT were significantly higher than bone scintigraphy, CT and $^{18}F$-FDG PET (P<0.05). On segment-based analysis, the sensitivity of CT, bone scintigraphy, $^{18}F$-FDG PET and $^{18}F$-FDG PET/CT were 70.4%, 89.5%, 89.1% and 97.8%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The overall specificity and accuracy of the four modalities were 89.1%, 91.8%, 90.3%, 98.2% and 90.3%, 90.9%, 89.8%, 98.0%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The PPV and NPV were 89.8%, 87.6%, 85.6%, 97.2% and 85.6%, 93.2%, 92.8%, 98.6%, respectively. Three hundred and twelve lesions or segments were presented as lytic or sclerotic changes on CT images at the corresponding sites of increased $^{18}F$-FDG uptake. In lytic or mixed lesions, the sensitivity of $^{18}F$-FDG PET/CT and $^{18}F$-FDG PET were better than bone scintigraphy, while in osteoblastic lesions bone scintigraphy had a similar performance with $^{18}F$-FDG PET/CT but better than $^{18}F$-FDG PET alone. Conclusion: Our data allow the conclusion that $^{18}F$-FDG PET/CT is superior to planar bone scintigraphy, CT or $^{18}F$-FDG PET in detecting bone metastasis. $^{18}F$-FDG PET/CT may enhance our diagnosis of tumor bone metastasis and provide more information for cancer treatment.

Preparation of shaped non-polyelectrolyte hydrogel particles with decomposable and recyclable performance by vortex ring freezing

Bo Liu,Chao Li,Yicheng Wang,Jun Nie,Xiaoqun Zhu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-

The vortex-ring derived particles are new type of non-spherical hydrogel particles, which are prepared byimitating the process of vortex-ring in nature. However, the traditional existing vorticity ring derivedhydrogel particles were prepared by dropping polyelectrolyte solution into contra-ion coagulation bath,which limited the choice of materials. In this paper, a strategy of preparation non-spherical hydrogel particlesby using non-polyelectrolyte polyvinyl alcohol through the method of vortex ring was proposed. Based on the principle that the boraciated polyvinyl alcohol solution could be quickly gelled underalkaline condition, the spherical and various derivative shapes (red cell, bowl, dish, ring) of polyvinylalcohol vortex-ring derived hydrogel particles (PVHP) were prepared. The hydrogel particles have theability to assemble into different modules by dynamic borate bond in the hydrogel particles. Also, thisdynamic borate bond endowed the PVHP decomposable and recyclable performances. In addition,two-compartments, three-compartments and magnetic PVHP were also prepared, endowing PVHP withseveral functions. which opens up a new environmentally friendly way for the preparation of multifunctionalparticles.

Search-based Path Planning and Receding Horizon Based Trajectory Generation for Quadrotor Motion Planning

Bo Zhang,Pudong Liu,Wanxin Liu,Xiaoshan Bai,Awais Khan,Jianping Yuan 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

This paper proposes a receding horizon based motion planning method, which allows a sensoryconstrained quadrotor to dynamically plan obstacle-avoiding trajectories in unknown complex environments. First, a two-process search method is proposed to generate an initial feasible path satisfying the dynamics of the quadrotor. Second, the path smoothness is improved by solving a nonlinear optimization problem considering path safety and smoothness. Then, a uniform B-spline is used to interpolate the path with a receding horizon to achieve a safe and dynamically feasible trajectory with minimum trajectory time by solving an optimization problem. Finally, a time adjustment method is proposed based on the relationship between the distance of the B-spline trajectory and the obstacles. Extensive simulation results illustrate that the designed method doubles the safety range, defined as the minimum distance between the quadrotor and the obstacles, and consumes less than 70% of computational running time compared with the state-of-the-art. Outdoor flight experiments performed with a vision-based quadrotor show the satisfying performance of the motion planning approach.

Clinicopathologic Characteristics and Prognostic Factors in Patients with Operable HER-2 Overexpressing Breast Cancer

Liu, Ai-Na,Sun, Ping,Liu, Jian-Nan,Ma, Jin-Bo,Qu, Hua-Jun,Zhu, Hua,Yu, Cai-Yan,Zhang, Liang-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To study the relationship between clinical pathologic characteristics, treatment modalities and prognostic factors in HER-2 (Human Epidermal growth factor Receptor-2) overexpressed breast carcinoma. Materials and Methods: Major clinico-pathological factors including therapeutic modalities and survival status of 371 breast cancer patients with HER2 over-expression, teated at Yantai Yuhuangding Hospital from March of 2002 to December of 2010 were retrospectively studied, with special attention focused on survival-related factors. Results: The median age of the total 371 patients in this study was 48 years at time of diagnosis, among which, the leading pathological type was infiltrating ductal carcinoma (92.5%); 62.8% presented with a primary tomor larger than 2 cm in diameter at diagnosis, 51.0% had axillary lymph node (ALN) metastases; ER (Estrogen receptor)/PR (Progesterone receptor) double negative occured in 52.8% of cases, and PCNA (proliferation cell nuclear antigen) (+++) was found in 55.1%. HER-2 overexpressed patients were usually in advanced stage when the diagnosis was made (72.8% at stages IIA~IIIC). The prognosis and survival were assessed in 259 patients with complete follow-up data. 5-year DFS (disease-free survival) and OS (overall survival) rate was 68.0% and 78.0% respectively. Univariate analysis revealed that age, tumor size, ALN metastases, LVSI (lymph-vascular space involvement), PCNA status, hormonal therapy, chemotherapy cycles, and HER-2 overexpression, correlated closely with the prognosis. ALN metastases, LVSI, PCNA status and chemotherapy cycles were independent predictors of survival. Conclusions: HER-2 overexpressed breast cancer has special clinical and pathological characteristics, with advanced clinical stages and high rate of ER/PR double negative. Lymph node metastases, LVSI, PCNA and chemotherapy cycles are independent predictors of prognosis.

Whitening and Anti-oxidative Activities of Chemical Components Extracted from Branches of Sorbus alnifolia

Bo Shi Liu,김정은,이남호 대한화학회 2023 대한화학회지 Vol.67 No.2

In this study were evaluated the whitening and anti-oxidative activities from the extracts of Sorbus alnifolia branches, and identified the chemical structures of the active ingredients. In the whitening tests using α-MSH stimulated B16F10 melanoma cells, the 70% ethanol extract and n-butanol (n-BuOH) fractions concentration-dependently inhibited cellular melanogenesis and intracellular tyrosinase activities without causing cell toxicity. The total polyphenol content of n-BuOH and ethyl acetate (EtOAc) fractions were measured to be respectively 241.1 ± 1.1 and 222.9 ± 2.4 (mg/g GAE), and the total flavonoid con- tent of EtOAc fraction was 75.3 ± 2.0 (mg/g QE). Upon anti-oxidant studies with DPPH and ABTS+ radicals, potent radical scavenging activities were observed in the EtOAc and n-BuOH fractions. Moreover, in the study of cell protection efficacy using HaCaT keratinocytes damaged by H2O2, the EtOAc and n-BuOH fractions showed a very positive results on prevention of oxidative stress. Phytochemical studies for this extract resulted in the isolation of four compounds; 2-oxopomolic acid (1), euscaphic acid (2), epi-catechin (3), prunasin (4). These results suggested that the extract of S. alnifolia branches containing compounds 1-4 as natural ingredients could be used as whitening and anti-oxidant ingredients in cosmetic formulations.