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- 저자 : Jun Nie (검색결과 57 건)
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Jun-Jie Zhu,Zi-Ling Nie,Yin-Feng Zhang,Yi Han 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6
The mode-switching control of transient process is important to grid-connected 400 ㎐ solid-state power supply systems. Therefore, this paper analyzes the principle of on-grid and islanding operation of the system with or without local loads in the grid-connected process and provides a theoretical study of the effect of different switching sequences on the mode-switching transient process. The conclusion is that the mode switch (MS) must be turned on before the solid-state switch (STS) in the on-grid process and that STS must be turned off before the MS in the off-grid process. A strategy of mode-switching smooth control for transient process of the system is proposed, including its concrete steps. The strategy utilizes the average distribution of peak currents and the smooth adjustment of peak currents and phases to achieve a no-shock grid connection. The simulation and experimental results show that the theoretical analysis is correct and that the method is effective.
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Comparison of In Vitro Cell Transformation Assay Using Murine Fibroblasts and Human Keratinocytes
Jun-Ho Ahn,Sue Nie Park,Yung-Na Yum,Ji-Young Kim,Michael Lee 한국독성학회 2008 Toxicological Research Vol.24 No.1
The in vitro cell transformation assays (CTA) were performed using BALB/3T3 murine fibroblasts and HaCaT human keratinocytes in order to evaluate concordance between both in vitro CTAs and carcinogenicity with compounds differing in their genotoxic and carcinogenic potential. Six test articles were evaluated, two each from three classes of compounds: genotoxic carcinogens (2-amino-5-nitrophenol and 4-nitroquinoline-N-oxide), genotoxic noncarcinogens (8-hydroxyquinoline and benzyl alcohol), and nongenotoxic carcinogens (methyl carbamate and N-nitrosodiphenylamine). Any foci of size ≥ 2 mm regardless of invasiveness and piling was scored as positive in CTA with BALB/3T3. As expected, four carcinogens regardless of their genotoxicity had positive outcomes in two-stage CTA using BALB/3T3 cells. However, of the two genotoxic noncarcinogens, benzyl alcohol was positive CTA finding. We concluded that, of the 6 chemicals tested, the sensitivity for BALB/3T3 system was reasonably high, being 100%. The respective specificity for BALB/3T3 assay was 50%. We also investigated the correlation between results of BALB/3T3 assay and results from HaCaT assay in order to develop a reliable human cell transformation assay. However, evaluation of staining at later time points beyond the confluency stage did not yield further assessable data because most of HaCaT cells were detached after 2~3 days of confluency. Thus, after test article treatment, HaCaT cells were split before massive cell death began. In this modified protocol for this HaCaT system, growing attached colonies were counted instead of transformed foci 3 weeks since last subculture. Compared to BALB/3T3 assay, HaCaT assay showed moderate low sensitivity and high specificity. Despite these differences in specificity and sensitivity, both cell systems did exhibit same good concordance between in vitro CTA and rodent carcinogenicity findings (overall 83% concordant results). At present the major weakness of these in vitro CTA is lack of validation for regulatory acceptance and use. Thus, more controlled studies will be needed in order to be better able to assess and quantitatively estimate in vitro CTA data.
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Comparison of In Vitro Cell Transformation Assay Using Murine Fibroblasts and Human Keratinocytes
Ahn, Jun-Ho,Park, Sue-Nie,Yum, Yung-Na,Kim, Ji-Young,Lee, Michael Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.
The in vitro cell transformation assays (CTA) were performed using BALB/3T3 murine fibroblasts and HaCaT human keratinocytes in order to evaluate concordance between both in vitro CTAs and carcinogenicity with compounds differing in their genotoxic and carcinogenic potential. Six test articles were evaluated, two each from three classes of compounds: genotoxic carcinogens (2-amino-5-nitrophenol and 4-nitroquinoline-N-oxide), genotoxic noncarcinogens (8-hydroxyquinoline and benzyl alcohol), and nongenotoxic carcinogens (methyl carbamate and N-nitrosodiphenylamine). Any foci of size $\geq$2 mm regardless of invasiveness and piling was scored as positive in CTA with BALB/3T3. As expected, four carcinogens regardless of their genotoxicity had positive outcomes in two-stage CTA using BALB/3T3 cells. However, of the two genotoxic noncarcinogens, benzyl alcohol was positive CTA finding. We concluded that, of the 6 chemicals tested, the sensitivity for BALB/3T3 system was reasonably high, being 100%. The respective specificity for BALB/3T3 assay was 50%. We also investigated the correlation between results of BALB/3T3 assay and results from HaCaT assay in order to develop a reliable human cell transformation assay. However, evaluation of staining at later time points beyond the confluency stage did not yield further assessable data because most of HaCaT cells were detached after $2{\sim}3$ days of confluency. Thus, after test article treatment, HaCaT cells were split before massive cell death began. In this modified protocol for this HaCaT system, growing attached colonies were counted instead of transformed foci 3 weeks since last subculture. Compared to BALB/3T3 assay, HaCaT assay showed moderate low sensitivity and high specificity. Despite these differences in specificity and sensitivity, both cell systems did exhibit same good concordance between in vitro CTA and rodent carcinogenicity findings (overall 83% concordant results). At present the major weakness of these in vitro CTA is lack of validation for regulatory acceptance and use. Thus, more controlled studies will be needed in order to be better able to assess and quantitatively estimate in vitro CTA data.
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Effects of Cisplatin - DNA Adducts on RecA Mediated Strand Exchange Reaction
Je Nie Phue,Jun Seong Bae,Tae Ryong Hahn 생화학분자생물학회 1992 BMB Reports Vol.25 No.7
The effects of cisplatin-DNA adducts on RecA mediated strand exchange reactions were examined in vitro. E. coli RecA protein catalizes strand exchange reaction between linear duplex and homologous single stranded DNA molecules to produce nicked circular heteroduplex DNA which can be detected by agarose gel electrophoresis. Platinum binding to both of DNA substrates inhibits significantly strand exchange reaction mediated by RecA protein. The exchanged heteroduplex DNA products were drastically decreased when the linear duplex DNA was treated with cisplatin. Those were not detected when the single stranded DNA was treated with cisplatin under the same conditions. These results suggest that single stranded DNA is more sensitive to cisplatin than duplex and/or that platinum-DNA adducts in single strands are structurally different from those of duplex.
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Lijuan Nie,Dongyan Chen,Jun Hu 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.8
This paper is concerned with the problem of protocol-based sliding mode control for a class of uncertain discrete networked Markovian jump systems with stochastic perturbation and time-varying delays. An improved dynamic uniform quantizer for processing system output signals is proposed to mitigate communication constraints. Next, a Round-Robin protocol with zero-order holders is introduced in the communication channels from the controller to the actuators to reduce potential network congestion and collision. Then, in the output feedback case,a new sliding surface is designed based on the quantized output, and a mode-dependent sliding mode controller is constructed using protocol scheduling signals. Additionally, a sufficient condition is derived to ensure that the closed-loop system is asymptotically stable in the mean square on a specific sliding surface despite the existence of the time-varying delays. Subsequently, the reachability of the state trajectories is guaranteed, where a sufficient criterion is proposed by constructing new Lyapunov-Krasovskii functional as well as using the stability theory. Furthermore, the cone complementary linearization iteration algorithm is employed to tackle the non-convex problem during the controller design. Finally, a simulation example demonstrate the effectiveness and feasibility of the protocol-based sliding mode control method.
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Hyperspectral Image Unmixing for Classification and Recognition : An Overview
Mingyu Nie,Zhi Liu,Hui Xu,Xiaoyan Xiao,Fangqi Su,Jun Chang,Xiaomei Li 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.12
The limited resolution of image sensors and the complex diversity of nature, cause mixed pixel problems in hyperspectral technology. Such problems are common, and increase the complexity of hyperspectral image processing. Hyperspectral unmixing is crucial for hyperspectral image classification and recognition. In unmixing, the image signatures are represented as a linear combination of the basic materials. Unmixing is the process of decomposing a mixed pixel into constituent materials, and calculating the corresponding fractional abundance. If pure materials (end members) are present in an image, unmixing can be divided into two steps, namely, end member extraction and abundance decomposition. On the other hand, if there is no pure material, researchers have devised and investigated unsupervised and semi-supervised spectral unmixing technology. This article presents an overview of the state-of-the-art methods of hyperspectral unmixing and their extensions.
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Zhu, Jun-Jie,Nie, Zi-Ling,Zhang, Yin-Feng,Han, Yi The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6
The mode-switching control of transient process is important to grid-connected 400 Hz solid-state power supply systems. Therefore, this paper analyzes the principle of on-grid and islanding operation of the system with or without local loads in the grid-connected process and provides a theoretical study of the effect of different switching sequences on the mode-switching transient process. The conclusion is that the mode switch (MS) must be turned on before the solid-state switch (STS) in the on-grid process and that STS must be turned off before the MS in the off-grid process. A strategy of mode-switching smooth control for transient process of the system is proposed, including its concrete steps. The strategy utilizes the average distribution of peak currents and the smooth adjustment of peak currents and phases to achieve a no-shock grid connection. The simulation and experimental results show that the theoretical analysis is correct and that the method is effective.
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Ru(II) Polypyridyl Complexes-Sensitized TiO2 Nanotubes for Photoreduction of CO2 Aqueous Solution
Yan Nie,Chen Wang,Jun Li,Xiao-Xun Ma 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.12
Ruthenium (II) polypyridyl complexes modified titanium dioxide materials with anatase phase, the length of 10–100 nm, were synthesized by a hydrothermal method. Photocatalytic CO2 reduction experiments were performed in sensitized TiO2 aqueous suspensions under UV–Vis light in closed systems. And only methanol was detected in the liquid phase under the experiment condition. The effect of different photosensitizers content on the photoactivity of TiO2 was also studied, showing that Ru(BiDiPy)2(NCS)2 sensitized TiO2 was the optimal photocatalyst in transformation of CO2 to methanol. A possible mechanism for the photocatalytic reduction was also proposed in this paper.
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