[PB-0094] Genome-Wide Association Study of Leaf Chlorophyll Content Using High-Density 50K SNP Array in Peanut Accessions

Kunyan Zou(Kunyan Zou),Nayoung Kwon(Nayoung Kwon),Tae-Hwan Jun(Tae-Hwan Jun) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0095] Genome-Wide Association Study and Transcriptome Analysis of Salinity Tolerance in Peanuts(Arachis hypogaea L.)

Kunyan Zou(Kunyan Zou),Seung-Ah Han(Seung-Ah Han),Min-jae Choi(Min-jae Choi),Min-jeong Lee(Min-jeong Lee),Da-Hye Jeon(Da-Hye Jeon),Tae-Hwan Jun(Tae-Hwan Jun) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Dynamic response characteristics of an innovative turretless low motion FPSO hull in central GoM ultra-deep waters

Zou, Jun Techno-Press 2022 Ocean systems engineering Vol.12 No.2

In oil and gas industry, FPSO concept is the most popular hull form and ship shaped hull form dominants the FPSO market. Only a non-ship-shaped hull in operations with minor market shares is the cylindrical FPSO hull with medium to small storage capability. To add contracting options and competitions to reduce field development costs, an innovative turretless low motion hull, eco-FPSO, with 1MM bbls oil storage capacity and suitable for installing topsides modulars and equipping with regular SCRs, was first introduced in Zou (2020a). Dynamic characteristic responses of the eco-FPSO compared to the traditional SS-FPSO hull and DD-Semi platform are presented and discussed in this paper, suitability and feasibility of the proposed hull have been demonstrated and validated through extensive analyses in 10-yrp, 100-yrp and 1,000-yrp hurricanes in ultra-deepwater central GoM.

자기 공명 영상을 이용한 안면비대칭환자의 측두하악관절 원반의 형태와 위치에 관한 연구

Bingshuang Zou,김태우,최순철 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.5

본 연구는 안면비대칭이 있는 환자의 측두하악관절원반 형태와 위치를 평가하고 악관절내장증과 안명비대칭 간의 관계를 규명하기 위하여 시행되었다. 남자 10명 및 여자 27명으로 구성된 안명비대칭 환자 37명의 74개의 TMJ MRI를 사용하였으며, 대상 집단의 연령은 12세에서 44세까지의 범위로 평균 21.4세였다. 시상 및 두정 TMJ MRI를 최대감합위 및 최대개구위에서 채득한 후 부분전방변위, 전방변위, 회전 또는 측방 원반변위 등 원반변위의 종류를 기록하였으며, 원반의 모양, 위치, 그리고 원반의 변위 및 회전을 MRI tracing 상에서 판독하였다. 악관절내장증의 증상은 정상, 정복성 전방원반변위 (ADDR). 그리고 비정복성 전방원반변위(ADDNR)집단으로 분류하였으며, 환자는 양측성 정상. 편측 혹은 양측 내장증 집단으로 분류하였다. 약 70%의 환자에서 편측성 또는 양측성 내장증을 보였으며, 통계분석 결과 ADD. 특히 회전성 ADD가 변위측에서 더 높은 빈도로 나타났으나 반대측에서는 원반의 위치가 정상인 경우가 많았다 (p<0.01).변위측의 원반은 모양의 변형 및 하전방 변위를 유의하게 더 나타냈다. 그러나 반대쪽은 개구시 원반이 수직방향으로 과운동성이 관찰되었다. 이 연구는 안면비대칭 환자에서 ADD의 종류가 원반의 모양,경사,변위 각도,변위의 수직거리 및 회전각도와 연관이 있다는 것을 제시한다. The present study was conducted to examine the morphometrics and function of the disk on both sides among patients with facial asymmetry (FA) and to elucidate plausible correlations between internal derangement (ID) and FA. The sample was composed of 10 males and 27 females with FA. The disk status of all subjects was evaluated by biateral high resolution magnetic resonance scans in the sagittal (closed and open) and coronal (closed) planes. Five types of disk displacement were identified accordingly. The disk function was diagnosed as normal disk function, disk displacement with reduction, and disk displacement without reduction. The disk shape on sagittal MRI in closed position was classified as bi-concave, biplanar, funnel/hemiconvex, and deformed. The disk position, translation and rotation were also measured. The difference between the shifted side and non-shifted side was analyzed by statistical analysis. Approximately 70% of the patients in the present study showed unilateral or bilateral ID. It was found that anterior disk displacements (ADD), especially rotational ADD, occurred more frequently in the shifted side, while normal disk position was observed mainly in the non-shifted side(p<0.01). The disk of the shifted side showed significantly deformed configuration and inferior-anterior disk position. However, the disk of the non-deviated side showed hyper-mobility during jaw opening movement. These results demonstrate that in FA patients, the disks status of the shifted side is different from that of the non-shifted side, a phenomenon that could be correlated to facial asymmetry.

Substituting oat hay or maize silage for portion of alfalfa hay affects growth performance, ruminal fermentation, and nutrient digestibility of weaned calves

Yang Zou,XinPing Zou,XiZhi Li,Gang Guo,Peng Ji,Yan Wang,ShengLi Li,YaJing Wang,ZhiJun Cao 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = 79.79±0.38 kg) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.

Genetic Diversity and Population Structure of Peanut (Arachis hypogaea L.) Accessions from Five Different Origins

Zou, Kunyan,Kim, Ki-Seung,Lee, Daewoong,Jun, Tae-Hwan The Korean Society of Crop Science 2020 한국작물학회지 Vol.65 No.4

Peanut is an allotetraploid derived from a single recent polyploidization. Polyploidization has been reported to have caused significant loss in genetic diversity during the domestication of cultivated peanuts. Single nucleotide polymorphism (SNP)-based markers such as cleaved amplified polymorphic sequences (CAPS) derived from next-generation sequencing (NGS) have been developed and widely applied for breeding and genetic research in peanuts. This study aimed to identify the genetic diversity and population structure using 30 CAPS markers and 96 peanut accessions from five different origins. High genetic dissimilarities were detected between the accessions from Korea and those from the other three South American origins generally regarded as the origin of peanuts, while the accessions from Brazil and Argentina presented the lowest genetic dissimilarity. Based on the results of the present study, accessions from Korea have unique genetic variation compared to those from other countries, while accessions from the other four origins are closely related. Our study identified the genetic differentiation in 96 peanut accessions from five different origins, and this study also showed the successful application of SNP information derived from re-sequencing based on NGS technology.

Application of LDH-release assay to cellular-level evaluation of the toxic potential of harmful algal species.

Zou, Yanan,Kim, Daekyung,Yagi, Motoaki,Yamasaki, Yasuhiro,Kurita, Jun,Iida, Takaji,Matsuyama, Yukihiko,Yamaguchi, Kenichi,Oda, Tatsuya Japan Society for Bioscience, Biotechnology, and A 2013 Bioscience, biotechnology, and biochemistry Vol.77 No.2

<P>Lactate dehydrogenase (LDH)-release assay was applied to estimate the toxic potential of harmful algal species at the cellular level. African green monkey kidney (Vero), yellowtail fin epithelia (MJF), and rainbow trout gill (RTgill-W1) cells were used as target cells. A live cell suspension of Karenia mikimotoi (SUO-1) induced the release of LDH from these cell lines, while the activity of another strain, FUK, was much lower. The cell-free culture supernatants and ruptured cell suspensions of both strains of K. mikimotoi were less effective on LDH-release assay. Exposure experiments against abalone and shrimp revealed that SUO-1 showed much stronger lethal effects on these organisms than FUK. Among six phytoplankton species, three species known to be harmful algal species induced the release of LDH to different extents depending on the cell line, whereas the other three species, known to be non-toxic, showed no effects on any cell lines. These results suggest that LDH-release assay is a useful micro-plate assay for estimation of the toxic potential of harmful phytoplankton.</P>

Positive Association Between miR-499A>G and Hepatocellular Carcinoma Risk in a Chinese Population

Zou, Hong-Zhi,Zhao, Yan-Qiu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

A case-control study of the association of miR-499A>G rs3746444 with risk of hepatocellular carcinoma (HCC)was conducted. Patients with HCC and healthy control subjects were recruited for genotyping of miR-499A>G using duplex polymerase-chain-reaction with confronting-two-pair primer(PCR-RFLP) analysis. The MiR-499 GG genotype was associated with a decreased risk of HCC as compared with the miR-499 AA genotype (adjusted OR=0.74, 95%CI=0.24-0.96). Similarly, the GG genotype showed a 0.45-fold decreased HCC risk in a recessive model. The MiR-499 G allele was significantly associated with decreased risk of HCC among patients infected with HBV in a dominant model (OR=0.09, 95%CI= 0.02-0.29). In conclusion, the MiR-499A>G rs3746444 polymorphism is associated with HCC risk in the Chinese population, and may be useful predictive marker for CAD susceptibility.

Short-term Power Load Forecasting Based on Phase Space Reconstruction and EMD-ELM

Zou Hongbo,Yang Qinhe,Chen Junting,Chai Yanhui 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.5

With the increasing complexity of the world energy structure, the uncertainty of the power system increases significantly, and the accuracy of the short-term power load forecasting is of great significance to the safe, economical and reliable operation of the power system. In order to further improve the accuracy of short-term power load forecasting, this paper innovatively combines the theories such as phase space reconstruction, empirical mode decomposition, and extreme learning machine to establish a new short-term power load forecasting model based on phase space reconstruction and EMD-ELM. The prediction results show that the root means square error, average relative error and global maximum relative error of the short-term power load forecasting model based on phase space reconstruction and EMD-ELM are much smaller than other forecasting models, which verifies the correctness and effectiveness of the short-term load forecasting method.

Pyrethroid susceptibility status and functional analysis of cytochrome P450 CYP6 subfamily genes in field Aedes albopictus, in Jiangsu, China

Zou Feifei,Guo Qin,Shen Bo,Lv Yuan 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

Aedes albopictus with a worldwide distribution, has been a vector for more than 20 arboviruses such as dengue, chikugunya, Zika and West Nile, but insecticide resistance in A. albopictus has become more and more serious, as a result of improper application of pyrethroid insecticides for mosquito control. The P450 gene cluster is known to be involved in metabolic mechanisms of pyrethroid resistance in other mosquito species. However, little is known about metabolic resistance in field A. albopictus in China. In this study, we collected five different field strains and first revealed the larval and adult resistance status of A. albopictus to deltamethrin in Jiangsu province of China. A combination of comparative genomics and P450 gene cluster analysis was conducted to investigate the underlying CYP6-mediated metabolic resistance mechanisms against deltamethrin in these field mosquitoes. The significance of three CYP6 genes, AALF024122, AALF024124 and AALF018944 in deltamethrin resistance was identified in our study. Furthermore, RNA interference-mediated silencing of three genes all increased the sensitivity of mosquitoes to deltamethrin in vivo, showing that these genes may be viable biomarkers for pyre throid resistance management in field A. albopictus.