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- 저자 : Zhu Shuying (검색결과 4 건)
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Gelation Modification of Alginate Nonwoven Fabrics
Jie Liu,Yun Liu,Dagang Miao,Shuying Sui,Chuanjie Zhang,Ping Zhu 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8
Calcium alginate nonwoven fabrics were gelation-modified by two-stage with aqueous HCl solution and then ethanolic NaOH solution. The structure and crystallinity properties of the samples were characterized by FT-IR, SEM, and XRD. The preparation conditions and modification mechanism were investigated. The results indicated that the crystal structure of calcium alginate fibers was destroyed; the crystallinity and calcium ion content decreased after HCl treatment. This resulted from the formation of ester bonds among the hydrolytic molecules after NaOH treatment. The best gel performance was obtained at the HCl concentration of 0.05-0.1 wt% with the NaOH concentration in ethanol of 2-4 mol/l. The liquid absorption of nonwoven alginate fabrics increased by 145 %. The water capability increased by 2673 % after modification, while the thickness, mass per unit area, permeability, and tensile strength of nonwoven alginate fabrics changed little.
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Li Feifei,Li Yan,Wang Tao,Li Tianfeng,Zhu Shuying,Pei Pei,Tang Guanghui 한국곤충학회 2020 Entomological Research Vol.50 No.6
Atrijuglans hetaohei Yang (Lepidoptera: Gelechioidea), is one of the major pests that seriously damage the walnut fruits. Although the morphology and physiology of A. hetaohei have been widely studied, suitable reference genes for normalizing target gene expression have not been identified. In this study, the expressions of eight candidate reference genes including GAPDH, β-Tubulin, TATA-binding protein (TBP), Histone, Ubiquitin-conjugating enzyme 9 (UBC9), arginine kinase (AK), 28S and elongation factor 1 alpha (EF1α) in different developmental stages and various larval tissues of A. hetaohei were determined using reverse transcription-quantitative polymerase chain reaction. Moreover, the expression stability of the selected reference genes was evaluated by ΔCt method, BestKeeper, NormFinder, geNorm and ReFinder, a comprehensive software platform. These results demonstrated that the best reference genes were GAPDH and 28S at different developmental stages of A. hetaohei; and the optimum references in various larval tissues of A. hetaohei were 28S, Histone and TBP. Our study should be useful to analyze the expression profiles of target genes and form a solid foundation for future research on the understanding of the genes’ biological functions in A. hetaohei.
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The Effect of HPMC and CNC on the Structure and Properties of Alginate Fibers
Meiyu Ci,Jie Liu,Shenglong Shang,Zhiming Jiang,Ping Zhu,Shuying Sui 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10
Bio-composite alginate fibers with binary and ternary blends were prepared by using cellulose nanocrystal (CNC)and hydroxypropyl methylcellulose (HPMC) as composite fillers through wet-spinning method. Structural, thermal,mechanical properties and surface morphology of fibers were characterized by Fourier transform infrared spectroscopy (FTIR),X-ray diffraction (XRD), Thermogravimetric Analysis (TGA), Mechanical strength testing, Scanning ElectronMicroscopy (SEM). The thermal stability and mechanical performance of SA/HPMC and SA/HPMC/CNC composite fibersimproved as the increasing of crystallinity and intermolecular H-bonding interaction of the fibers. HPMC is helpful toimprove the extensibility and stiffness of alginate fibers, and CNC can further enhance the stiffness of SA/HPMC compositefibers. The tensile strength, elongation at break, the initial modulus and work at break of SA/HPMC/CNC composite fiberswere superior to those of alginate fibers. Roughness of surface and tensile section of SA/HPMC and SA/HPMC/CNCcomposite fibers got increased. Water absorbency and salt resistance were significantly improved.
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SMURF1-mediated ubiquitination of ARHGAP26 promotes ovarian cancer cell invasion and migration
Xuri Chen,Shaoyun Chen,Yao Li,Yanling Gao,Shuying Huang,Hongping Li,Yuanfang Zhu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Rho GTPase-activating protein 26 (ARHGAP26) is a negative regulator of the Rho family that converts the small GTPbinding protein RhoA (GTP-RhoA) to its inactive GDP-bound form and is a putative tumor suppressor gene associated with cell growth and migration. Here, the involvement of ARHGAP26 in ovarian cancer cell proliferation and migration was investigated. In this study, low ARHGAP26 expression was observed in ovarian cancer tissues and was associated with a poor overall survival and higher β-catenin expression in patients with ovarian cancer. A2780 and HEY cells with ARHGAP26 upregulation showed decreased cell proliferation, migration, and invasion, along with decreased GTPRhoA, β-catenin, VEGF, MMP2, and MMP7 expression. ARHGAP26 upregulation in A2780 cells also inhibited lung metastasis in vivo. SKOV3 cells with ARHGAP26 downregulation demonstrated an inverse effect, which was inhibited by ARHGAP26 overexpression or DKK1, an antagonist of the β-catenin pathway. SMURF1, an E3 ubiquitin ligase, interacted with and induced ubiquitination of ARHGAP26. ARHGAP26 upregulation in SKOV3 cells significantly inhibited SMURF1 upregulation-induced cell migration and invasion. Overall, SMURF1-mediated ubiquitination of ARHGAP26 may promote invasion and migration of ovarian cancer cells via the β-catenin pathway.
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