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Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3
BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.
Yi-Kang Sun,Xiao-Long Li,Qiao Wang,Bo-Yang Zhou,An-Qi Zhu,Chuan Qin,Le-Hang Guo,Hui-Xiong Xu 대한초음파의학회 2022 ULTRASONOGRAPHY Vol.41 No.2
Purpose: This prospective study explored the value of synchronous tele-ultrasound (US) to aid doctors inexperienced in US with breast US examinations.Methods: In total, 99 patients were enrolled. Two trainee doctors who were inexperienced in US (trainee A [TA] and trainee B [TB]) and one doctor who was an expert in US completed the US examinations sequentially. TA completed the US examinations independently, while TB was instructed by the expert using synchronous tele-US. Subsequently, the expert performed on-site US examinations in person. Separately, they selected the most clinically significant nodule as the target nodule. Consistency with the expert and image quality were compared between TA and TB to evaluate tele-US. Furthermore, TB and the patients evaluated tele-US through questionnaires.Results: TB demonstrated higher consistency with the expert in terms of target nodule selection than TA (93.3% vs. 63.3%, P<0.001). TB achieved good inter-observer agreement (ICC, >0.75) with the expert on five US features (5/9, 55.6%), while TA only did so for one (1/9, 11.1%) (P=0.046). TB’s image quality was higher than TA’s in gray value, time gain compensation, depth, color Doppler adjustment, and the visibility of key information (P=0.018, P<0.001, P<0.001, P=0.033, and P=0.006, respectively). The comprehensive assessment score was higher for TB than for TA (3.96±0.82 vs. 3.09±0.87, P<0.001). Tele-US was helpful in 69.7% of US examinations and had a training effect in 68.0%. Furthermore, 63.6% of patients accepted tele-US and 60.6% were willing to pay.Conclusion: Tele-US can help doctors inexperienced in US to perform breast US examinations.
A Noninjection Reaction Route to CuInSe2 Nanocrystals with Triethanolamine as the Complexing Agent
Qi-Yi Zhang,Wen-Long Liu,Meng-Qiang Wu,Ru-Chao Zhou,Li-Dan Yan,Shu-Ren Zhang 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.12
The chalcopyrite-type CuInSe_2 is a remarkable material for thin film solar cells owing to its electronic structure and optical response. Single-phase sphere-like CuInSe_2 nanocrystallite particles were prepared by a facile noninjection method with triethanolamine as the complexing agent and the solvent simultaneously. The period of the reaction was the key to form single-phase CuInSe_2 nanocrystals at 240 ^oC. TEM, XRD, XPS, EDX investigations were performed to characterize the morphology and the detailed structure of as-synthesized CuInSe_2 nanocrystals. All of the analysis results proved that the synthesized nanocrystals were pure phase and close to the stoichiometric ratio rather than a simple mixture. The band gap of the obtained CuInSe_2nanocrystals was 1.03 ± 0.03 eV.
Tradeoff Control of Multi-exposure Lithography for SU-8 Photochemical Reaction Channel Formation
Qi-Ming Chen,Jin-Yun Zhou,Yi-Ming Hu,Qi Zheng 한국바이오칩학회 2020 BioChip Journal Vol.14 No.4
A method involving multi-exposure with low-power is presented to fabricate an SU-8 mold based on a digital micromirror device (DMD) maskless lithography with an LED source at a wavelength of 405 nm. The SU-8 mold is used for the fabrication of a PDMS concentration gradient generator (CGG). During a one-time exposure, it is easy to over-expose at the top and under-expose at the bottom of a thick SU-8, which then forms a T-shaped structure. This is more obvious for high-absorption coefficients such as 365 nm. We found that by taking advantage of the partially absorbed and partially transmissive characteristics of the 405-nm wavelength, multi-exposure can form an effective photochemical reaction channel in SU-8 and can solve the problem mentioned above. However, excessive exposure will cause the linewidth to increase, therefore, it is necessary to find a tradeoff for the number of multi-exposure times. For a 55-μm thick SU-8, the tradeoff threshold is found to be 25. Three types of SU-8 CGG molds were fabricated at this threshold. The results indicate that the actual profile of the SU-8 mold shows good agreement with the design profile without any T-shaped structures.
Tian-qi Guo,Yi-di Zhang,Wen-jing Luo,Xue Li,Yan-min Zhou,Jing-hui Zhao,Jing-hui Zhao 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.2
Genotoxicity and effect on early stage proliferation of osteoprogenitor cells of titanium surfaces modified by heptylamine functionalization through Plasma Enhanced Chemical Vapor Deposition (PECVD) were evaluated in current study. Mouse embryo osteoprogenitor MC3T3-E1 cells were grown on titanium surfaces of all the groups. The CCK-8 assay showed the growths of MC3T3-E1 cells on treated titanium disks were not inhibited compared with those on pure titanium groups. Result of Single Cell Gel Electrophoresis (SCGE) test indicated the treated groups showed no significant differences in tail length, tail DNA%, and tail moment compared with pure titanium group. Rate of micronucleus, nucleoplamic buds and nuclear blebs showed no significant differences between treated groups and pure titanium group. Our research exhibited that: The amino-group functionalized titanium surface has neither genotoxicity nor detrimental effects on early-stage proliferation towards osteoprogenitor cells in vitro, thus offer a theoretic support for the clinical use of such functionalized titanium as implant surface.
Zhang, Yi,Wang, Peng,Zhou, Xing-Chun,Bao, Guo-Qiang,Lyu, Zhuo-Ming,Liu, Xiao-Nan,Wan, Shao-Gui,He, Xian-Li,Huang, Qi-Chao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11
Background: Hypoxia-inducible factor $1{\alpha}$ (HIF-$1{\alpha}$) plays an important role in regulating cell survival and angiogenesis, which are critical for tumor growth and metastasis. Genetic variations of HIF1A have been shown to influence the susceptibility to many kinds of human tumors. Increased expression of HIF-$1{\alpha}$ has also been demonstrated to be involved in tumor progression. However, the prognostic value of single nucleotide polymorphisms (SNPs) inthe HIF1A gene remains to be determined in most cancer types, including colorectal cancer (CRC). In this study, we sought to investigate the predictive role of HIF1A SNPs in prognosis of CRC patients and efficacy of chemotherapy. Materials and Methods: We genotyped two functional SNPs in HIF1A gene using the Sequenom iPLEX genotyping system and then assessed their associations with clinicopathological parameters and clinical outcomes of 697 CRC patients receiving radical surgery using Cox logistic regression model and Kaplan Meier curves. Results: Generally, no significant association was found between these 2 SNPs and clinical outcomes of CRC. In stratified analysis of subgroup without adjuvant chemotherapy, patients carrying CT/TT genotypes of rs2057482 exhibited a borderline significant association with better overall survival when compared with those carrying CC genotype [Hazard ratio (HR), 0.47; 95% confidence interval (95% CI): 0.29-0.76; P < 0.01]. Moreover, significant protective effects on CRC outcomes conferred by adjuvant chemotherapy were exclusively observed in patients carrying CC genotype of rs2057482 and in those carrying AC/CC genotype of rs2301113. Conclusions: Genetic variations in HIF1A gene may modulate the efficacy of adjuvant chemotherapy after surgery in CRC patients.
Entanglement Dynamics of Electron Spins in Quantum Dots Under a Nonuniform Magnetic Field
Feng-Xue Zhou,Yi-Hong Qi,Yue-Ping Niu,Shang-Qing Gong,Jun Qian,Ting Yu,Franco Nori 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.60 No.8
We investigate entanglement of two coupled electron spins in quantum dots (QDs) in the presence of an inhomogeneous magnetic field. The great effects of the inhomogeneous field are discussed for the dynamics of entanglement. It is shown that due to the system's symmetry, the inhomogeneity of the field does not affect the evolution of entanglement for Φ-type Bell state, while it plays a key role for Ψ-type Bell state. For the maximal entangled Bell states, the field is positive for the entanglement dynamics. The mean field can increase the entanglement revival for Φ-type Bell state while the inhomogeneous field can promote the entanglement revival for Ψ-type Bell state. For the unentangled initial states, the field is destructive for the entanglement generation induced by the coupling of the two spins.
Chu, Jia-Qi,Jing, Kai-Peng,Gao, Xiang,Li, Peng,Huang, Rui,Niu, Yan-Ru,Yan, Shou-Quan,Kong, Jun-Chao,Yu, Cai-Yuan,Shi, Ge,Fan, Yi-Ming,Lee, Young-Ha,Zhou, Yu,Quan, Juan-Hua Landes Bioscience 2017 Cell Cycle Vol.16 No.5
<P>Autophagy and apoptosis are critical for controlling Toxoplasma gondii (T. gondii) infection. T. gondii infection during pregnancy can damage the fetus and cause birth defects; however, the molecular mechanisms of this process are poorly understood. This study aims to determine the activities of autophagy and apoptosis as well as their regulatory mechanisms during T. gondii infection by using human umbilical cord mesenchymal stem cells (hUC-MSCs) as a model of congenital diseases. LC3B, a hallmark protein of autophagy was incrementally upregulated with the infection duration, whereas p62 was downregulated in T. gondii-infected hUC-MSCs. Concurrent to this result, the invasion of T. gondii into hUC-MSCs increased in a time-dependent manner. The expression levels of Bcl-2 family proteins including Bcl-2, Bcl-xL, Bim, Bax, Bid and Bak were not altered; however, Mcl-1 levels in hUC-MSCs were dramatically decreased upon T. gondii infection. In addition, at 24h post-infection, cleaved PARP and cleaved caspase-3 protein levels were elevated in hUC-MSCs. Importantly, Mcl-1 overexpression reduced the levels of autophagy- and apoptosis-related proteins in T. gondii-infected hUC-MSCs. Mcl-1 proteins were primarily expressed in the fraction containing mitochondria and strongly interacted with Beclin-1 under normal conditions; however, these interactions were remarkably attenuated by T. gondii infection. These results suggest that mitochondrial Mcl-1 is an essential signaling mediator regulating the activation of autophagy and apoptosis during T. gondii infection.</P>