Comparison of stomach microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing

Wen‑kui Sun,Yanyue Li,Chi Cheng,Yi‑hui Chen,Kai Zeng,Xiaohui Chen,Yiren Gu,Rui Liu,Xuebin Lv,Rong Gao 한국유전학회 2018 Genes & Genomics Vol.40 No.9

MiRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in different physiological processes, yet little is known about the stomach microRNAome (miRNAome) of the Tibetan pig. The objective of this experiment was to investigate differentially expressed stomach miRNAs participating in digestion. Firstly, we isolated total RNA by Trizol reagent from three Tibetan and three Yorkshire purebred pigs stomach samples at 90-day-old. Secondly, a comprehensive analysis of Tibetan and Yorkshire pig stomach miRNAomes was performed by small RNA sequencing in the Illumina HiSeq 2000 system. Finally, SYBR Green Real-time RT-PCR was performed to validate the differentially expressed miRNAs. We identified 318 unique miRNAs, 260 were co-expressed in both libraries, 17 and 31 miRNAs were specifically expressed in Tibetan and Yorkshire pigs respectively. Fifty six differentially expressed miRNAs were identified by the identifying differentially expressed genes 6 (IDEG6). Kyoto encyclopedia of genes and genomes analysis revealed that some of the differentially expressed miRNAs were associated with protein and fat digestion. Two differentially expressed miRNAs (miR-214-3p and ssc-un39) participating in the digestion of lipid were identified. Additionally, qRT-PCR results suggested that a higher expression of miR-214-3p in the Tibetan pig stomach could lead to relatively lower expression of calcium-dependent phospholipase A2, which is an enzyme important for the digestion of glycerol phospholipid. This study has delineated the different stomach miRNAs expression patterns of Tibetan and Yorkshire pigs, which would help explain the regulatory mechanisms of miRNAs in digestion of Tibetan pigs, and contribute to utilize a the unique digestion merits of Tibetan pig in future porcine hybridization breeding.

Observer-based Adaptive Fuzzy Control for Strict-feedback Nonlinear Systems with Prescribed Performance and Dead Zone

Wen Zeng,Zhigang Li,Chuang Gao,Libing Wu 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.5

This paper proposes an observer-based fuzzy controller for strict-feedback nonlinear systems. A fuzzy state observer is designed to estimate unmeasurable system states via a fuzzy logic system to approximate the unknown nonlinear functions of the system. A tangent prescribed performance function is utilized to gather the tracking error in a small neighborhood of the origin. The control input is designed to deal with the dead-zone property of the system. The proposed controller can guarantee that all the signals in the closed-loop system are semi-globally, uniformly, and ultimately bounded. Simulation results demonstrate the effectiveness of the proposed controller

Coevolution between Human's Anticancer Activities and Functional Foods from Crop Origin Center in the World

Zeng, Ya-Wen,Du, Juan,Pu, Xiao-Ying,Yang, Jia-Zhen,Yang, Tao,Yang, Shu-Ming,Yang, Xiao-Meng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Cancer is the leading cause of death around the world. Anticancer activities from many functional food sources have been reported in years, but correlation between cancer prevalence and types of food with anticancer activities from crop origin center in the world as well as food source with human migration are unclear. Hunger from food shortage is the cause of early human evolution from Africa to Asia and later into Eurasia. The richest functional foods are found in crop origin centers, housing about 70% in the world populations. Crop origin centers have lower cancer incidence and mortality in the world, especially Central Asia, Middle East, Southwest China, India and Ethiopia. Asia and Africa with the richest anticancer crops is not only the most important evolution base of humans and origin center of anticancer functional crop, but also is the lowest mortality and incidence of cancers in the world. Cancer prevention of early human migrations was associated with functional foods from crop origin centers, especially Asia with four centers and one subcenter of crop origin, accounting for 58% of the world population. These results reveal that coevolution between human's anticancer activities associated with functional foods for crop origin centers, especially in Asia and Africa.

Correlation between rearing temperature and the dual cellulolytic system of Coptotermes formosanus Shiraki and its intestinal microenvironment

Wen-Hui Zeng,Zhi-Qiang Li,Bing-Rong Liu,Qiu-Jian Li,Jun-Hong Zhong 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

The lower termite, Coptotermes formosanus Shiraki, is an important model species for studying the dual cellulolytic system of termites. The effect of rearing temperature on the interaction between the intestinal symbiont community and the host in lignocelluloses digestion was investigated by assaying the activity and gene expression of cellulase, and monitoring the population size of intestinal protozoan community, which was followed by rearing C. formosanus at a temperature gradient (15–37 °C) for 3 d. The results showed that the rearing temperature of C. formosanus had a much greater impact on cellulase activity and gene transcription of the intestinal symbiont than on the host. The transcriptional variations of endogenous and symbiotic cellulase genes presented mutual complement and restrained when C. formosanus living in high temperature environment (32 and 37 °C). Additionally, the optimum survival temperature of Pseudotrichonympha grassii Koidzumi (22 °C) was found lower than that of Holomastigotoides mirabile Koidzumi (28 °C) and Spirotrichonympha leidyi Koidzumi (28 °C).The optimum rearing temperature for cellulase activity and protozoan survival of C. formosanus was found much lower than its optimum temperature for feeding. These findings provide a novel glimpse into host-symbiotic mutualism in lower termite digestion.

Strategies of Functional Food for Cancer Prevention in Human Beings

Zeng, Ya-Wen,Yang, Jia-Zheng,Pu, Xiao-Ying,Du, Juan,Yang, Tao,Yang, Shu-Ming,Zhu, Wei-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Functional food for prevention of chronic diseases is one of this century's key global challenges. Cancer is not only the first or second leading cause of death in China and other countries across the world, but also has diet as one of the most important modifiable risk factors. Major dietary factors now known to promote cancer development are polished grain foods and low intake of fresh vegetables, with general importance for an unhealthy lifestyle and obesity. The strategies of cancer prevention in human being are increased consumption of functional foods like whole grains (brown rice, barley, and buckwheat) and by-products, as well some vegetables (bitter melon, garlic, onions, broccoli, and cabbage) and mushrooms (boletes and Tricholoma matsutake). In addition some beverages (green tea and coffee) may be protective. Southwest China (especially Yunnan Province) is a geographical area where functional crop production is closely related to the origins of human evolution with implications for anticancer influence.

Expression of HERC4 in Lung Cancer and its Correlation with Clinicopathological Parameters

Zeng, Wen-Li,Chen, Yao-Wu,Zhou, Hui,Zhou, Jue-Yu,Wei, Min,Shi, Rong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Growing evidence suggests that the members of the ubiquitin-proteasome system (UPS) are important for tumorigenesis. HERC4, one component, is a recently identified ubiqutin ligase. However, the expression level and function role of HERC4 in lung cancer remain unknown. Our objective was to investigate any correlation between HERC4 and development of lung cancer and its clinical significance. Materials and Methods: To determine HERC4 expression in lung cancer, an immunohistochemistry analysis of a tissue microarray containing samples of 10 lung normal tissues, 15 pulmonary neuroendocrine carcinomas, 45 squamous epithelial cancers and 50 adenocarcinomas was conducted. Receiver operating characteristic (ROC) curve analysis was applied to obtain a cut-off point of 52.5%, above which the expression of HERC4 was regarded as "positive". Results: On the basis of ROC curve analysis, positive expression of HERC4 was detected in 0/10 (0.0%) of lung normal tissues, in 4/15 (26.7%) of pulmonary neuroendocrine carcinomas, in 13/45 (28.9%) of squamous epithelial cancers and in 19/50 (38.0%) of adenocarcinomas. It showed that lung tumors expressed more HERC4 protein than adjacent normal tissues (${\chi}^2$=4.675, p=0.031). Furthermore, HERC4 positive expression had positive correlation with pT status (${\chi}^2$=44.894, p=0.000), pN status (${\chi}^2$=43.628, p=0.000), histological grade (${\chi}^2$=7.083, p=0.029) and clinical stage (${\chi}^2$=72.484, p=0.000), but not age (${\chi}^2$=0.910, p=0.340). Conclusions: Our analysis suggested that HERC4 is likely to be a diagnostic biomarker for lung cancer.

Improved Channel Estimation Algorithms based on a Proposed Signal Model for PUCCH Format 3 in LTE-A

Xiaoping Zeng,Ya Wen,Zhiming Wang,Yuemei Jin 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.1

SORTD (Space Orthogonal-Resource Transmit Diversity) has already become a better way of transmit diversity for PUCCH format 3 in LTE-A [1]. SORTD technology lies in the characteristic that each antenna port transmits signal by using different cyclic shift [2] to make orthogonal each other. Using the SORTD’s feature of incoherence in different antennas, this paper proposes a special form of signal model. Based on this proposed model, we propose a low complexity channel estimator based on LS and cubic spline interpolation, and then we put a LS and DCT algorithm to improve the interpolation performance. Finally, for more accurate estimator we drive the third approach based on MMSE and DCT. A series of theoretical researches and simulation results show that all of them are suitable for the proposed signal model even in the environment of high-speed movement; especially the algorithms with DCT have a better performance.

QTLs of Cold Tolerance-Related Traits at the Booting Stage for NIL-RILs in Rice Revealed by SSR

Ya Wen Zeng,Shu Ming Yang,Hong Cui,Xiao Juan Yang,Li Ming Xu,Juan Du,Xiao Ying Pu,Zi Chao Li,Zai Quan Cheng,Xing Qi Huang 한국유전학회 2009 Genes & Genomics Vol.31 No.2

QTLs for cold tolerance-related traits at the booting stage using balanced population for 1525 recombinant inbred lines of near-isogenic lines (viz. NIL-RILs for BC5F3 and BC5F4 and BC5F5) over 3 years and two locations by backcrossing the strongly cold-tolerant landrace (Kunmingxiaobaigu) and a cold-sensitive cultivar (Towada) was analyzed. In this study, 676 microsatellite markers were employed to identify QTLs conferring cold tolerance at booting stage. Single marker analysis revealed that 12 markers associated with cold tolerance on chromosome 1, 4 and 5. Using a LOD significance threshold of 3.0, compositive interval mapping based on a mixed linear model revealed eight QTLs for 10 cold tolerance-related traits on chromosomes 1, 4, and 5. They were tentatively designated qCTB-1-1, qCTB-4-1, qCTB-4-2, qCTB-4-3, qCTB-4-4, qCTB-4-5, qCTB-4-6, and qCTB-5-1. The marker intervals of them were narrowed to 0.3-6.8 cM. Genetic distances between the peaks of the QTL and nearest markers varied from 0 to 0.04 cM. We were noticed in some traits associated cold tolerance, such as qCTB-1-1 for 5 traits (plant height, panicle exsertion, spike length, blighted grains per spike and spikelet fertility), qCTB-4-1 for 8 traits (plant height, node length under spike, leaf length, leaf width, spike length, full grains per spike, total grains per spike and spikelet fertility), qCTB-4-2 for 3 traits (spike length, full grains per spike and spikelet fertility), qCTB-5-1 for 5 traits (plant height, panicle exsertion, blighted grains per spike, full grains per spike and spikelet fertility). The variance explained by a single QTL ranged from 0.80 to 16.80%. Three QTLs (qCTB-1-1, qCTB-4-1, qCTB-4-2) were detected in two or more trials. Our study sets a foundation for cloning cold-tolerance genes and provides opportunities to understand the mechanism of cold tolerance at the booting stage.

Investigation on Machining Characteristic of Pneumatic Wheel based on Softness Consolidation Abrasives

Xi Zeng,Shi-ming Ji,Ming-sheng Jin,Da-peng Tan,Jue-hui Li,Wen-tao Zeng 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

In order to improve material removal rate of laser hardening workpiece and also make machining tool suitable for free-form surface,a new method based on the softness consolidation abrasives (SCA) is put forward, which means the abrasives are consolidated onthe outer layer of pneumatic wheel to achieve the softness-machining. Binder selecting test shows that acidic silicone is proved tosuit for consolidating particles in cutting process. Combined with robot, the machining system has been established. The machiningeffects of SCA are investigated when it faces with workpiece of different hardness. According to the Preston predicted model, thesimulation results of stress and velocity are proven by analysis of average roughness in the contact region. The contrastive machiningexperimental results show that SCA can supply high cutting stress for material removal and fit for freeform surface’s machining byself-adjustment of flexible body.

Two new sesquiterpenoids from endophytic fungus J3 isolated from Mangrove Plant Ceriops tagal

Yan-Bo Zeng,Hai-Gang Gu,Wen-Jian Zuo,Li-Li Zhang,Hong-Jin Bai,Zhi-Kai Guo,Peter Proksch,Wen Li Mei,Hao Fu Dai 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5

Two new sesquiterpenoids, named 2a-hydroxyxylaranolB (1) and 4b-hydroxyxylaranol B (2), togetherwith a known diterpenoid 3,4-seco-sonderianol (3) wereisolated from the fermentation of endophytic fungus J3 ofCeriops tagal. Their structures were elucidated based onspectroscopic methods including 1D and 2D NMR(HMQC, 1H-1H COSY and HMBC). All compounds wereevaluated for their cytotoxic activities by MTT method,and compound 3 exhibited cytotoxic activities againstK562, SGC-7901, and BEL-7402 cell lines.