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Single-cell sequencing techniques from individual to multiomics analyses
Yukie Kashima,Yoshitaka Sakamoto,Keiya Kaneko,Masahide Seki,Yutaka Suzuki,Ayako Suzuki 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and transcriptomic methods, and examples of their applications. For the integration of multilayered data sets, such as the transcriptome data derived from single-cell RNA sequencing and chromatin accessibility data derived from single-cell ATAC-seq, there are several computational integration methods. We also describe single-cell experimental methods for the simultaneous measurement of two or more omics layers. We can achieve a detailed understanding of the basic molecular profiles and those associated with disease in each cell by utilizing a large number of single-cell sequencing techniques and the accumulated data sets.
51V-NMR Study of the Quasi-one-dimensional Antiferromagnet BaCo2V2O8
Yukiichi Ideta,Yu Kawasaki,Yutaka Kishimoto,Takashi Ohno,Yoshitaka Michihiro,Zhangzhen He,Yutaka Ueda,Mitsuru Itoh 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3
We have investigated the magnetic properties of quasi-one-dimensional antiferromagnetBaCo2V2O8 by the measurements of 51V-NMR spectrum. With decreasing temperature, Kk showsa broad peak around 30 K signaling the 1D short-range order in the Co2+ spin chain. The estimatedWeiss temperature Θ = −105 K, hyperfine coupling constants Akhf = 2.1 kOe/µB for H k c and Ahf= 2.6 kOe/µB for H ⊥ c at 13 kOe are comparable to those at 70.6 kOe. These results suggest thatstatic magnetic properties in the paramagnetic state are nearly independent of external magneticfield, although the ground N´eel state is suppressed by the application of external magnetic fieldabove Hc = 39 kOe along c-axis. In the antiferromagnetic state below TN = 5.4 K, the doublerectangle-like powder pattern was observed, indicating two V sites exposed to different size of internalfields, HA1 = 2.1 and HA2 = 3.8 kOe. The internal fields at V sites are explained by takinginto account of the classical and pseudo-dipolar fields from Co2+ magnetic moments.