http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Yunfeng Hu (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Yunfeng He,Xiaohou Wu,Wei Tang,Daiyin Tian,Chunli Luo,Zhikang Yin,Hu Du 대한의학회 2008 Journal of Korean medical science Vol.23 No.5
The oncogenic isoform of the p63 protein, delta Np63 ( Np63), plays an important role in the pathogenesis of many epithelial carcinomas, and emerging evidences suggest that Np63 is a promising drug target. However, the functions of Np63 in transitional cell carcinoma of bladder (TCCB) are poorly defined. In this study, a Np63 shRNA expression vector was transfected into TCCB cell line 5637 and cell cycling, cell proliferation and protein expression were assessed by flow cytometry and 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-dimethyl tetrazolium bromide (MTT) assay, and immunohistochemistry, respectively. The Np63 shRNA expression vector was also injected into 5637 cell xenograft tumors in nude mice, and tumor size was measured, tumor tissue morphology was assessed by immunohistopathology and transmission electron microscopy. In the in vitro study, Np63 shRNA transfection caused successful Np63 gene silencing and resulted in significant arrest of cell cycling and cellular proliferation (p<0.05) as well as cyclin D1 expression. In the nude mouse xenograft model, Np63 shRNA greatly inhibited tumor growth, induced tumor cell apoptosis (p<0.05) and resulted in cyclin D1 downregulation. Our data suggest that Np63 may play an oncogenic role in TCCB progression through promoting cell survival and proliferation. Intratumoral administration of Np63-specific shRNA suppressed tumor Np63 expression and cellular proliferation while promoted tumor cellular apoptosis, and therefore inhibited tumor growth and improved survival of xenograft-bearing mice, which was not accompanied by significant signs of systemic toxicity.
-
Jian Yang,Jie Li,Yunfeng Hu,Lizhen Li,Lijuan Long,Fazuo Wang,Si Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
High value utilization of byproduct of slaughterhousesis regarded as beneficial to both economy andenvironment. A hemoglobin degrading protease PA720was purified from fermentation broth of a Porites luteaassociated strain Streptomyces rutgersensis SCSIO 11720(SCSIO 11720) and exhibited maximal activity at 70°C,pH 10.5. The isolated strain was identified by genotypicmethod and 16 s RNA gene sequence was deposited inGenBank under accession No. KC414842. The enzymeexhibited excellent thermostability since it was stable at 70°Cwith t1/2 value of 1,386.3 min. Kinetic and thermodynamicstudy showed the activation energy (Ea), ΔG* (free energy),ΔH* (enthalpy) and ΔS* (entropy) for protease deactivationwere 62.71 kJ/mol, 74.26 kJ/mol, 59.86 kJ/mol, and-41.97 J/mol/K, respectively. Highest specific activity ofprotease PA720 was observed when using casein (9,953 U/mg)and hemoglobin (9,854 U/mg) as substrates. Hemoglobinhydrolysate prepared by protease PA720 showed significantantibacterial activity towards Escherichia coli, Staphylococcusaureus and Bacillus subtilis, indicating this protease couldbe used as an instrumental enzyme for production ofhemoglobin-based antibacterial peptides. Based on partialamino acid sequences of the enzyme PA720, the full geneencoding this protease was obtained by degenerate primerPCR and has been deposited in GenBank under accessionNo. KC414842. The deduced amino acid sequence exhibitshomology with other microbial thermophilic proteases insubtilisin family.
-
Xuesong Shang,Gongren Hu,Chi He,Jinping Zhao,Fuwang Zhang,Ya Xu,Yunfeng Zhang,Jianrong Li,Jinsheng Chen 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1
An effective and applicable method for the regeneration of used commercial full-scale honeycomb monolith catalysts in China (V2O5–WO3/TiO2) is firstly reported. The de-ionized water and dilute sulfuric acid solution with a relatively low concentration (pH = 2) are potent reagent to regenerated the catalysts used for about 30,000 h in coal-fired power plant. All samples were systematically characterized by TEM,N2 adsorption/desorption, EDX, FT-IR and XPS techniques. The evaluation results reveal that the activity of the used catalysts can be obviously improved after regeneration processes. Moreover, the regenerated catalysts possess superior activity under different gas hourly space velocity (GHSV), and SO2 oxidation rate is lower than 1% in all cases. The regeneration method can remove the deposits, restore active sites and prevent nanoparticles aggregation as well as active phases leaching. Overall, these processes have promised as effective approaches for industrial V2O5–WO3/TiO2 catalysts regeneration.
-
Nannan Wang,Yifu Zhang,Tao Hu,Yunfeng Zhao,Changgong Meng 한국물리학회 2015 Current Applied Physics Vol.15 No.4
Ultrahigh-aspect-ratio V2O5 nanowires were successfully prepared using [VO(O2)2(OH2)]- as the starting material by a template-free hydrothermal route without the addition of organic surfactant or inorganic ions. The prepared samples were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer-Emmet-Teller (BET), cyclic voltammetry (CV) and galvanostatic charge-discharge (GCD). The results revealed that the peroxovanadium (V) complexes can be easily transformed to V2O5 nanowires by this hydrothermal route. The uniform nanowires were with width about 50 nm and length about dozens of micron. The BET analysis showed the V2O5 nanowires had a high specific surface area of 25.6 m2 g-1. The synthesized V2O5 nanowires performed a high capacitance of 351 F g-1 when used as supercapacitor electrode in 1 mol L-1 LiNO3.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
