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( Yun Cheng Wu ),( Jun Yuan ),( Waseem Raza ),( Qi Rong Shen ),( Qi Wei Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.10
Bacillus amyloliquefaciens strain NJZJSB3 has shown antagonism of several phytopathogens in vitro, especially Sclerotinia sclerotiorum. Both the broth culture and cell suspension of strain NJZJSB3 could completely protect the detached leaves of canola (Brassica napus) from S. sclerotiorum infection. In pot experiments, the application of strain NJZJSB3 cell suspension (108 CFU/ml) decreased the disease incidence by 83.3%, a result similar to commercially available fungicide (Dimetachlone). In order to investigate the potential biocontrol mechanisms of strain NJZJSB3, the nonvolatile antifungal compounds it produces were identified as iturin homologs using HPLC-ESI-MS. Antifungal volatile organic compounds were identified by gas chromatography-mass spectrometry. The detected volatiles toluene, phenol, and benzothiazole showed antifungal effects against S. sclerotiorum in chemical control experiments. Strain NJZJSB3 also produced biofilm, siderophores and cell-wall-degrading enzymes (protease and β-1,3-glucanase). These results suggest that strain NJZJSB3 can be a tremendous potential agent for the biological control of sclerotinia stem rot.
Yun-Qi Wang,Ji-Ye Cai 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
While biodegradable, biocompatible poly(L-lactic acid) (PLLA) is a popular material for the manufacture of tissue engineering scaf-folds, its surface properties are not particularly suitable for cell growth. In the present study, poly(L-lactic acid) (PLLA) lms were fab-ricated by a solution casting method and their surfaces were subsequently modied by NaOH hydrolysis. The changes of surfaceproperties were characterized by contact-angle measurement and atomic force microscopy (AFM). Mouse L929 broblasts were usedas model cells to evaluate the cell anity of PLLA before and after surface modication. The results showed that the hydrophilicity20 nm to between 40 and 200 nm. The adhesion and growth of mouse L929 broblasts on modied lms were signicantly improved.Thus, improved hydrophilicity and increased surface roughness at 40200 nm could signicantly enhance cell adhesion and growth onmodied PLLA that could be useful for applications in tissue engineering.
Tradeoff Control of Multi-exposure Lithography for SU-8 Photochemical Reaction Channel Formation
Qi-Ming Chen,Jin-Yun Zhou,Yi-Ming Hu,Qi Zheng 한국바이오칩학회 2020 BioChip Journal Vol.14 No.4
A method involving multi-exposure with low-power is presented to fabricate an SU-8 mold based on a digital micromirror device (DMD) maskless lithography with an LED source at a wavelength of 405 nm. The SU-8 mold is used for the fabrication of a PDMS concentration gradient generator (CGG). During a one-time exposure, it is easy to over-expose at the top and under-expose at the bottom of a thick SU-8, which then forms a T-shaped structure. This is more obvious for high-absorption coefficients such as 365 nm. We found that by taking advantage of the partially absorbed and partially transmissive characteristics of the 405-nm wavelength, multi-exposure can form an effective photochemical reaction channel in SU-8 and can solve the problem mentioned above. However, excessive exposure will cause the linewidth to increase, therefore, it is necessary to find a tradeoff for the number of multi-exposure times. For a 55-μm thick SU-8, the tradeoff threshold is found to be 25. Three types of SU-8 CGG molds were fabricated at this threshold. The results indicate that the actual profile of the SU-8 mold shows good agreement with the design profile without any T-shaped structures.
Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.
( Yun Tao Liu ),( Jun Sun ),( Zeyu Luo ),( Sheng Qi Rao ),( Yu Jie Su ),( Yan Jun Yang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4
This is the first report on using Catathelasma ventricosum for production of fruiting body and lignocellulosic enzymes. To improve the laccase activity and productivity of mushroom, the substrate was added with different supplements (eight aromatic compounds, Mn2+, and Cu2+). Based on the results, all these supplements can improve the laccase activity and productivity of C. ventricosum, and it seems that there is a critical value of laccase activity that affects the productivity of C. ventricosum. In addition, when Penicillium decumbens was inoculated into the substrate that had been cultivated with C. ventricosum for 20 days, the highest values of laccase activity, FPA activity, and productivity of C. ventricosum were obtained. Moreover, the laccase activity showed a positive correlation with the productivity of C. ventricosum. Finally, the effect of Mn2+, Cu2+, and P. decumbens on laccase activity was investigated by response surface methodology (RSM).
Correction strategy of fuel injection quantity during pilot-main injection for common rail system
Yun Bai,Yuanqi Gu,Qi Lan,Liyun Fan,Xiuzhen Ma 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.6
Common rail system is a key technology of energy saving and emission reduction for modern diesel engines. Multiple injection, as one of the most interesting features of common rail system, allows both optimal fuel consumption and exhaust emissions. In order to explore the method for controlling the fluctuation of fuel injection quantity during multiple injection, experiments have been carried out in this paper, focusing on pilot-main injection. The high pressure fuel circuits of the system have been equivalent to a spring-mass vibration system. Comparison with the experiment shows that the proposed fluctuation equation can reasonably predict the fluctuation characteristics of main injection quantity with pilot-main injection interval. The correction control strategy for the main injection quantity fluctuation has been proposed, in which the relative damping coefficient, rail pressure, pilot-main injection interval and main injection pulse width are chosen as the input variables. The experimental results with different rail pressure and main injection quantity show that the fluctuation of injection quantity during pilotmain injection can be controlled effectively by the proposed correction strategy. The maximum average fluctuation of main injection quantity decreases by as much as 44.66 %.