Suppression of long non-coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis through regulation of MELK targeted by miR-142-5p

Yu‑hua Xie,Jiao Hu 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Glioma has the chief type of primary brain tumors worldwide. The glioma may be controlled by regulatorsincluding some lncRNAs, miRNAs, and proteins. Objective Our study aims to discover the underlying mechanism for lncPCAT19/miR-142-5p/MELK axis in gliomaprogression. Methods The clinical samples were from patients with gliomas in our Hospital. Hematoxylin–eosin staining (H&E) wasapplied to determine the clinical pathological changes. Real time PCR was performed to measure the levels of lncPCAT19,miR-142-5p, MELK, and expression of other genes. Western blot was conducted to detect the protein level of MELK. RIPassay was performed to analyze the interaction between lncPCAT19 and miR-142-5p, and dual-luciferase reporter assay wasused to determine the binding site between lncPCAT19 and miR-142-5p. CCK-8, colony formation assay, flow cytometry,and trans-well assay were carried out to confirm cell proliferation, colony formation, apoptosis, and invasion, respectively. Results LncPCAT19 was increased in cancer tissues. Then, lncPCAT19 could interact with and down-regulate miR-142-5p. Knockdown of lncPCAT19 distinctly inhibited tumor growth in vivo. Interfering lncPCAT19/overexpression of miR-142-5pdecreased glioma cell proliferation, colony formation and invasion, and promoted cell apoptosis by down-regulating expressionof Cyclin B1, CDK2, N-cadherin, Bcl-2, and by up-regulating expression of Bax and E-cadherin. Moreover, overexpressionof lncPCAT19 overturned tumor-suppressing role of miR-142-5p in cells. Additionally, lncPCAT19 and miR-142-5psynergistically regulated expression of MELK. In conclusion, lncPCAT19 enhanced glioma development via increasingMELK by performing as a sponge of miR-142-5p. Conclusions LncPCAT19 promotes glioma progression by sponging miR-142-5p to upregulate MELK levels. Thus, lncPCAT19/miR-142-5p/MELK signaling would be a potential target for glioma treatment.

Experimental Study of Mechanical Behavior of Passive Loaded Piles Adjacent to Piled Foundation

Yu Xie,Shao-he Zhang,De-quan Zhou 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.10

For combined piled foundation, passive loaded piles will be subjected to horizontal force when piled foundation is loaded, and horizontal force may cause the piles horizontal displacement, instability or even failure. With inclined bedrock nearby, the mechanical behavior of passive loaded piles will be more complex. In order to study the mechanical behavior of passive loaded piles under such condition, laboratory model test was carried out, during repeatedly loading/unloading cycles. Axial force, side friction, bending moment, and lateral soil pressure were analyzed. The study is based on model test, and more related studies are needed before being applied to practical conditions. The results show as follows: Axial force distribution of the piles changes with the increase of load and becomes stable under high load. This phenomenon is related to the expansion of potential slip surface in piled foundation. The passive loaded pile that is close to load boundary has only one neutral point which is located in the lower part of the pile, and its side friction has a relatively stable direction. On the contrary, the direction of side friction of the pile that is far from load boundary changes frequently. Bending moment curves of passive loaded piles vary with the change of the distance between the piles and load boundary, and they intersect at the lower part of the pile. Bending moment is basically negative above the intersection and gradually turns into positive below it. The maximum lateral soil pressure of passive loaded pile is triple that of the adjacent soil, indicating that passive loaded pile can effectively limit the horizontal deformation of piled foundation. But when the pile is too far from load boundary, it will lose the ability to limit the horizontal deformation of piled foundation.

Impact of playout buffer dynamics on the QoE of wireless adaptive HTTP progressive video

Xie, Guannan,Chen, Huifang,Yu, Fange,Xie, Lei Electronics and Telecommunications Research Instit 2021 ETRI Journal Vol.43 No.3

The quality of experience (QoE) of video streaming is degraded by playback interruptions, which can be mitigated by the playout buffers of end users. To analyze the impact of playout buffer dynamics on the QoE of wireless adaptive hypertext transfer protocol (HTTP) progressive video, we model the playout buffer as a G/D/1 queue with an arbitrary packet arrival rate and deterministic service time. Because all video packets within a block must be available in the playout buffer before that block is decoded, playback interruption can occur even when the playout buffer is non-empty. We analyze the queue length evolution of the playout buffer using diffusion approximation. Closed-form expressions for user-perceived video quality are derived in terms of the buffering delay, playback duration, and interruption probability for an infinite buffer size, the packet loss probability and re-buffering probability for a finite buffer size. Simulation results verify our theoretical analysis and reveal that the impact of playout buffer dynamics on QoE is content dependent, which can contribute to the design of QoE-driven wireless adaptive HTTP progressive video management.

DEGSO : Hybrid Group Search Optimizer with Differential Evolution Operator

Yu Xie,Chunxia Zhao,Haofeng Zhang,Debao Chen 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6

In standard group search optimizer (GSO) algorithm, scroungers will converge to the similar position if the producer cannot find a better position than the old one in a number of successive iterations and the group may suffer from the premature convergence. In this paper, a hybrid GSO with differential evolution (DE) operator named DEGSO is proposed to enhance the diversity of standard group search optimizer. In this method, the standard GSO algorithm and the DE operator alternate at the odd iterations and at the even iterations. The results of the experiments indicate that DEGSO is competitive to some other evolutionary computation (EA) algorithms.

Transjugular intrahepatic portosystemic shunt for pyrrolidine-alkaloid-induced hepatic sinusoidal obstruction syndrome in a patient with situs inversus totalis and portal vein thrombus

Yu-Guan Xie,Chen Xu,Hai-Bin Shi,Sheng Liu,Wei Yang,Wei Tian,Wei-Zhong Zhou 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1

We here report a rare case of pyrrolidine-alkaloid-induced hepatic sinusoidal obstruction syndrome (PA-HSOS) complicated with situs inversus totalis and portal vein thrombus (PVT). The patient had abdominal distention and ascites, which were not responsive to conservative medical treatment or peritoneal drainage. Transjugular intrahepatic portosystemic shunt (TIPS) was successfully performed in the patient via the left internal jugular vein approach. After the procedure, abdominal distension and ascites gradually resolved. The TIPS procedure could be considered as a useful treatment for PA-HSOS patients with PVT.

An Electric-Field Coupled Power Transfer System with a Double-sided LC Network

Xie, Shi-Yun,Su, Yu-Gang,Zhou, Wei,Zhao, Yu-Ming,Dai, Xin The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1

Electric-field coupled power transfer (ECPT) systems employ a high frequency electric field as an energy medium to transfer power wirelessly. Existing ECPT systems have made great progress in terms of increasing the transfer distance. However, the topologies of these systems are complex, and the transfer characteristics are very sensitive to variations in the circuit parameters. This paper proposes an ECPT system with a double-sided LC network, which employs a parallel LC network on the primary side and a series LC network on the secondary side. With the same transfer distance and output power, the proposed system is simpler and less sensitive than existing systems. The expression of the optimal driving voltage for the coupling structure and the characteristics of the LC networks are also analyzed, including the transfer efficiency, parameter sensitivity and total harmonic distortion. Then, a design method for the system parameters is provided according to these characteristics. Simulations and experiments have been carried out to verify the system properties and the design method.

Short-term Overseas Study Tours by Secondary Students in China : What to Do and What to Gain?

Xie, Rou Yu (Rosa),Kim, Yong Hee 부산대학교 중국연구소 2017 Journal of China Studies Vol.20 No.4

JCAD deficiency attenuates activation of hepatic stellate cells and cholestatic fibrosis

Li Xie,Hui Chen,Li Zhang,Yue Ma,Yuan Zhou,Yong-Yu Yang,Chang Liu,Yu-Li Wang,Ya-Jun Yan,Jia Ding,Xiao Teng,Qiang Yang,Xiu-Ping Liu,Jian Wu 대한간학회 2024 Clinical and Molecular Hepatology(대한간학회지) Vol.30 No.2

Background/Aims: Cholestatic liver diseases including primary biliary cholangitis (PBC) are associated with active hepatic fibrogenesis, which ultimately progresses to cirrhosis. Activated hepatic stellate cells (HSCs) are the main fibrogenic effectors in response to cholangiocyte damage. JCAD regulates cell proliferation and malignant transformation in nonalcoholic steatoheaptitis-associated hepatocellular carcinoma (NASH-HCC). However, its participation in cholestatic fibrosis has not been explored yet. Methods: Serial sections of liver tissue of PBC patients were stained with immunofluorescence. Hepatic fibrosis was induced by bile duct ligation (BDL) in wild-type (WT), global JCAD knockout mice (JCAD-KO) and HSC-specific JCAD knockout mice (HSC-JCAD-KO), and evaluated by histopathology and biochemical tests. In situ-activated HSCs isolated from BDL mice were used to determine effects of JCAD on HSC activation. Results: In consistence with staining of liver sections from PBC patients, immunofluorescent staining revealed that JCAD expression was identified in smooth muscle α-actin (α-SMA)-positive fibroblast-like cells and was significantly up-regulated in WT mice with BDL. JCAD deficiency remarkably ameliorated BDL-induced hepatic injury and fibrosis, as documented by liver hydroxyproline content, when compared to WT mice with BDL. Histopathologically, collagen deposition was dramatically reduced in both JCAD-KO and HSC-JCAD-KO mice compared to WT mice, as visualized by Trichrome staining and semi-quantitative scores. Moreover, JCAD deprivation significantly attenuated in situ HSC activation and reduced expression of fibrotic genes after BDL. Conclusions: JCAD deficiency effectively suppressed hepatic fibrosis induced by BDL in mice, and the underlying mechanisms are largely through suppressed Hippo-YAP signaling activity in HSCs.

Prognostic Values of VEGF and Endostatin with Malignant Pleural Effusions in Patients with Lung Cancer

Zhang, Yu,Yu, Li-Ke,Lu, Guo-Jun,Xia, Ning,Xie, Hai-Yan,Hu, Wei,Hao, Ke-Ke,Xu, Chun-Hua,Qian, Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Aims: Angiogenesis is important in malignant pleural effusion (MPE) formation and it is regulated by a number of pro- and anti-angiogenic cytokines. The purpose of this study was to evaluate the prognostic value of angiogenic factor vascular endothelial growth factor (VEGF) and angiogenesis inhibitor endostatin in lung cancer patients with MPE, and investigate the relationship between these two kinds of agent. Methods: Using enzyme-linked immunoadsorbent assay, the concentrations of VEGF and endostatin were measured in pleural effusions (PE) and serum from a total of 70 lung cancer patients with MPE and 20 patients with tuberculosis. Results: Compared to patients with tuberculosis, the levels of VEGF and endostatin in both PE and serum were significantly higher in patients with lung cancer. There were statistically significant correlations between VEGF levels in PE and serum (r=0.696, p<0.001), endostatin levels in PE and serum (r=0.310, p=0.022), and VEGF and endostatin levels in PE (r=0.287, p=0.019). Cox multivariate analysis revealed that elevated pleural VEGF and endostatin levels and serum endostatin level were independent predictors of shorter overall survival. Conclusion: Both pro- and anti-angiogenic factors are likely contributors to PE formation. Our results suggest that the levels of VEGF and endostatin in PE, together with endostatin in serum, may be potential prognostic parameters for lung cancer patients with MPE.

Lack of Association Between CYP1A1 Polymorphisms and Risk of Bladder Cancer: a Meta-analysis

Lu, Yu,Zhang, Xiao-Lian,Xie, Li,Li, Tai-Jie,He, Yu,Peng, Qi-Liu,Deng, Yan,Wang, Jian,Qin, Xue,Li, Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Background: The effects of CYP1A1 gene polymorphisms on the risk of bladder cancer (BC) remain controversial. We carried out a meta-analysis to clarify the role of CYP1A1 gene polymorphisms in BC. Material and Methods: A comprehensive literature search was conducted up to November 20, 2013. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to estimate the strength of the association. Meta-regression, subgroup analysis, sensitivity analysis and publication bias were also performed. Results: Eight studies involving 1,059 BC cases and 1,061 controls were included. The meta-analysis showed that there was no significant association between the two common mutations of CYP1A1 and BC risk. For the I1e462Val A/G polymorphism with GG vs. AA the OR was 1.47 (95 % CI= 0.70-3.07, P =0.308). For the MspI T/C polymorphism, though a slight trend was found this was not statistically nonsignificant (CC vs.TT, OR = 1.24, 95 % CI= 0.98-1.58, P =0.078). Subgroup analyses by ethnicity also found no obvious association between CYP1A1 and BC risk. Conclusion: The present meta-analysis suggests that CYP1A1 polymorphism is not associated with bladder cancer risk.