The Synergism of Human Lactobacillaceae and Inulin Decrease Hyperglycemia via Regulating the Composition of Gut Microbiota and Metabolic Profiles in db/db Mice

Li Peifan,Tong Tong,Wu Yusong,Zhou Xin,Zhang Michael,Liu Jia,She Yongxin,Li Zuming,Li Yongli 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.12

This study aimed to evaluate the effects of Limosilactobacillus fermentum and Lactiplantibacillus plantarum isolated from human feces coordinating with inulin on the composition of gut microbiota and metabolic profiles in db/db mice. These supplements were administered to db/db mice for 12 weeks. The results showed that the Lactobacillaceae coordinating with inulin group (LI) exhibited lower fasting blood glucose levels than the model control group (MC). Additionally, LI was found to enhance colon tissue and increase the levels of short-chain fatty acids. 16S rRNA sequencing revealed that the abundance of Corynebacterium and Proteus, which were significantly increased in the MC group compared with NC group, were significantly decreased by the treatment of LI that also restored the key genera of the Lachnospiraceae_NK4A136_group, Lachnoclostridium, Ruminococcus_ gnavus_group, Desulfovibrio, and Lachnospiraceae_UCG-006. Untargeted metabolomics analysis showed that lotaustralin, 5-hydroxyindoleacetic acid, and 13(S)-HpODE were increased while L-phenylalanine and L-tryptophan were decreased in the MC group compared with the NC group. However, the intervention of LI reversed the levels of these metabolites in the intestine. Correlation analysis revealed that Lachnoclostridium and Ruminococcus_gnavus_group were negatively correlated with 5-hydroxyindoleacetic acid and 13(S)-HpODE, but positively correlated with L-tryptophan. 13(S)-HpODE was involved in the "linoleic acid metabolism". L-tryptophan and 5-hydroxyindoleacetic acid were involved in "tryptophan metabolism" and "serotonergic synapse". These findings suggest that LI may alleviate type 2 diabetes symptoms by modulating the abundance of Ruminococcus_gnavus_group and Lachnoclostridium to regulate the pathways of "linoleic acid metabolism", "serotonergic synapse", and" tryptophan metabolism". Our results provide new insights into prevention and treatment of type 2 diabetes.

Removal of chlorinated organic solvents from hydraulic fracturing wastewater by bare and entrapped nanoscale zero-valent iron

Lei, Cheng,Sun, Yuqing,Khan, Eakalak,Chen, Season S.,Tsang, Daniel C.W.,Graham, Nigel J.D.,Ok, Yong Sik,Yang, Xin,Lin, Daohui,Feng, Yujie,Li, Xiang-Dong Elsevier 2018 CHEMOSPHERE - Vol.196 No.-

<P><B>Abstract</B></P> <P>With the increasing application of hydraulic fracturing, it is urgent to develop an effective and economically feasible method to treat the large volumes of fracturing wastewater. In this study, bare and entrapped nanoscale zero-valent iron (nZVI) were introduced for the removal of carbon tetrachloride (CT) and 1,1,2-trichloroethane (TCA) in model high-salinity fracturing wastewater. With increasing ionic strength (<I>I</I>) from Day-1 (<I>I</I> = 0.35 M) to Day-90 (<I>I</I> = 4.10 M) wastewaters, bare nZVI presented significantly lower removal efficiency of CT (from 53.5% to 38.7%) and 1,1,2-TCA (from 71.1% to 21.7%) and underwent more serious Fe dissolution from 1.31 ± 1.19% in Day-1 to 5.79 ± 0.32% in Day-90 wastewater. Particle aggregation induced by high ionic strength was primarily responsible for the lowered performance of nZVI due to less available reactive sites on nZVI surface. The immobilization of nZVI in alginate with/without polyvinyl alcohol provided resistance to particle aggregation and contributed to the superior performance of entrapped nZVI in Day-90 wastewater for 1,1,2-TCA removal (62.6–72.3%), which also mitigated Fe dissolution (4.00–4.69%). Both adsorption (by polymer matrix) and reduction (by immobilized nZVI) were involved in the 1,1,2-TCA removal by entrapped nZVI. However, after 1-month immersion in synthetic fracturing wastewater, a marked drop in the reactivity of entrapped nZVI for 1,1,2-TCA removal from Day-90 wastewater was observed with significant release of Na and total organic carbon. In summary, bare nZVI was sensitive to the nature of the fracturing wastewater, while the use of environmentally benign entrapped nZVI was more promising for wastewater treatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Increasing ionic strength decreased nZVI reactivity and increased Fe dissolution. </LI> <LI> Entrapping nZVI in polymer matrix improved reactivity and limited Fe dissolution. </LI> <LI> Entrapped nZVI removed model chlorinated organic via both adsorption and reduction. </LI> <LI> Aging process was mitigated by polymer matrix but still inhibited nZVI reactivity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Bradyrhizobium spp. and Sinorhizobium fredii are Predominant in Root Nodules of Vigna angularis, a Native Legume Crop in the Subtropical Region of China

Li Li Han,En Tao Wang,Yang Li Lu,Yong Fa Zhang,Xin Hua Sui,Wen Feng Chen,Wen Xin Chen 한국미생물학회 2009 The journal of microbiology Vol.47 No.3

Adzuki bean (Vigna angularis) is an important legume crop native to China, but its rhizobia have not been well characterized. In the present study, a total of 60 rhizobial strains isolated from eight provinces of China were analyzed with amplified 16S rRNA gene RFLP, IGS-RFLP, and sequencing analyses of 16S rRNA, atpD, recA, and nodC genes. These strains were identified as genomic species within Rhizobium, Sinorhizobium, Mesorhizobium, Bradyrhizobium, and Ochrobactrum. The most abundant groups were Bradyrhizobium species and Sinorhizobium fredii. Diverse nodC genes were found in these strains, which were mainly co-evolved with the housekeeping genes, but a possible lateral transfer of nodC from Sinorhizobium to Rhizobium was found. Analyses of the genomic and symbiotic gene backgrounds showed that adzuki bean shared the same rhizobial gene pool with soybean (legume native to China) and the exotic Vigna species. All of these data demonstrated that nodule formation is the interaction of rhizobia, host plants, and environment characters.

Characteristics of circRNAs expression profiles in the piglets intestine induced by oxidative stress

Li Zhi-xin,Chen Wei,Qin Ming,Wang Li-xue,Zeng Yong-qing 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Backgroud: Oxidative stress (OS) can affect the expression of key genes and destroy the intestinal structure. However, it is unclear how OS regulates the expression of circular RNAs (circRNAs), microRNAs (miRNAs) and mRNAs. Objective: The aim of this study was to examine the expression of circRNAs, miRNAs and mRNAs exposed to OS. Methods: Piglets were exposed to diquat (DQ), a herbicide, and the activity of antioxidant enzymes and the morphology of the intestine were investigated. We utilized whole transcriptome sequencing to examine the global expression of circRNAs, miRNAs and mRNAs in the jejunum. Results: Compared to controls, 751 circRNAs, 731 miRNAs and 164 mRNAs were differentially expressed in diquat-treated piglets. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that oxidative phosphorylation, RNA degradation and ubiquitin-mediated proteolysis were closely associated with OS. Conclusions: Our results indicated that diquat-induced OS alters the intestinal structure, resulting in the differential expression of circRNAs, miRNAs and mRNAs in the jejunum of piglets. Meanwhile, OS weakened the enzyme antioxidant system in serum of piglets. Our results provide a foundation for further studies on the mechanisms involved in the response to OS in the jejunum.

Extractive bioconversion of xylan for production of xylobiose and xylotriose using a PEG6000/sodium citrate aqueous two-phase system

Xin Li,Zhina Lian,Bo Dong,Yong Xu,Qiang Yong,Shiyuan Yu 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.9

Aqueous two-phase system (ATPS) was applied for extraction bioconversion of xylan by xylanase from Trichoderma viride. Phase diagrams for poly (ethylene glycol) (PEG) and sodium citrate were determined at room temperature. The ATPS composed of 12.99% (w/w) PEG6000 and 12.09% (w/w) sodium citrate was favorable for partition of xylanase and used for extraction bioconversion of xylan. Batch hydrolysis demonstrated that higher concentrations of xylobiose and xylotriose were obtained in the PEG6000/sodium citrate ATPS compared to those in the aqueous system. These results present the potential feasibility of production of xylo-oligosaccharides by extraction bioconversion in ATPS.

Utilization of Seaweed Derived Ingredients as Potential Antioxidants and Functional Ingredients in the Food Industry: An Overview

Yong-Xin Li,김세권 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6

Recently, a great deal of interest has been developed to isolate novel bioactive compounds from marine resources because of their numerous health beneficial effects. Among marine resources, marine algae are valuable sources of structurally diverse bioactive compounds with potential to be used against artificial food ingredients. This mini-review focuses on seaweed-derived bioactive compounds such as phlorotannins, sulfated polysaccharides, carotenoid pigments, and fucosterol with their potential antioxidant effect in the food industry as functional ingredients.

Three-dimensional Seismic Displacement Analysis of Rock Slopes based on Hoek-Brown Failure Criterion

Yong-xin Li,Xiao-li Yang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.11

Seismic force is one of the main factors resulting in the slip failure of steep rock slopes. In this paper, the seismic displacement analysis of rock slopes is carried out by virtue of the kinematical theorem of limit analysis and the Newmark method. It is assumed that the slope has a curvilinear cone-shaped failure mechanism and its materials are subjected to the Hoek-Brown failure criterion. The generalized tangential technique is employed to obtain equivalent parameters from the Hoek-Brown criterion. According to the three-dimensional failure mechanism, the internal energy dissipation and external work are respectively calculated, and the effect of horizontal seismic force is included by using the equivalent pseudo-static method. Subsequently, the yield acceleration is calculated for three-dimensional failure mechanism, and the results are shown to be valid when compared with the previous solutions. Parameter analysis is used to represent how the Hoek-Brown criterion parameters impact on the yield acceleration. The failure mechanism is also obtained during the process of limit analysis, and a dimensionless coefficient is adopted to investigate the influence of it on the seismic displacements of slopes. The results of calculations are given for a series of actual seismic waves and compared with the results calculated from empirical formula.

Inhibition of Vascular Endothelial Growth Factor (VEGF) Induced Angiogenic Responses by Phlorotannin Compound

Yong-Xin LI,Yong LI,Zhong-Ji QIAN,BoMi RYU,Se-Kwon KIM 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10

Stability Analysis of Crack Slope Considering Nonlinearity and Water Pressure

Yong-xin Li,Xiao-li Yang 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.6

The soil slopes tend to induce the appearance of tension cracks at the top of slope under various factors, which poses an adverse impact on slope stability. Based on Power-Law nonlinear failure criterion, kinematical approach is adopted in this paper to investigate the effects of underground water and vertical tension cracks on stability of slopes. Notice that the tension cracks tend to propagate vertically, and thus a novel failure mechanism composed by the tension crack and logarithmic spiral shear slide plane is postulated. Theoretically, the worst position of crack propagation makes the stability factor minimal, and the failure surfaces can be obtained under different conditions through optimization. According to the proposed mechanism and nonlinear Mohr-Coulomb strength criterion, the stability factor and safety coefficient of slopes are derived. Subsequently, sensitivity analysis with respect to specific examples is conducted to analyze the influence of tension cracks, nonlinear strength parameters and pore water pressure on the slope stability. The results indicate that slope stability weakens gradually at the presence of tension cracks, and the changing degree is more evident with the increase of the slope angle. Meanwhile, nonlinear parameters have more significant impact on the safety factor with the decreasing value of the slope angle. However, the bigger the slope angle, the greater the height of tension cracks, and therefore the more obvious influence of pore water pressure on slope stability. When the slope angle approaches to be vertical, the height of tension cracks increases sharply. As a matter of fact, the height of tension cracks is highly related to geometrical conditions, strength parameters and water effect, which build a mutual relationship to determine slope stability.

Intermedins A and B; New Metabolites from Schisandra propinqua var. intermedia

Li, Hong-Mei,Lei, Chun,Luo, Yong-Ming,Li, Xiao-Nian,Li, Xiao-Lei,Pu, Jian-Xin,Zhou, San-Yun,Li, Rong-Tao,Sun, Han-Dong 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A new dibenzocyclooctadiene lignan, intermedin A (1), and a new natural bisabolane sesquiterpenoid, intermedin B (2), were isolated from the aerial parts of Schisandra propinqua var. intermedia. Their structures were elucidated on the basis of extensive spectroscopical analysis.