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성인 지역사회 폐렴의 원인 미생물에 대한 전향적 다기관 연구 : 마이코플라즈마와 클라미디아의 역할
주철현,윤하정,남정현,문미선,조영걸,우준희,강재명,신완식,김양리,김민자,정희진,이환종,김유겸 대한감염학회 2001 감염 Vol.33 No.1
Background : The purpose of this study is to develop a rapid and sensitive method to detect M. pneumoniae and C. pneumoniae. In addition, we prospectively investigated the prevalence of M. pneumoniae and C. pneumoniae infections in community-acquired pneumonia. Methods : The nasopharyngeal aspirates were collected from truly 1997 to April 2000. PCR or nested PCR techniques were used to detect causative agents. RFLP and nucleotide sequence analysis were performed to study molecular epidemiology. Furthermore, feasibility of one-step technique to detect two microorganisms was tested by multiplex PCR. Results : Of 250 clinical specimens, 23 (9.2%) specimens were positive for M. pneumoniae and 33 (13.2%) for C. pneumoniae, including one dual-infected specimen. Most M. pneumoniae infection occurred during the colder months of the year. C. pneumoniae infection was predominantly found from December to February. Conclusion : The results indicate that M. pneumoniae and C. pneumoniae infections are frequently found from patients with community-acquired pneumonia. (Korean J Infect Dis 33: 15-23, 2001)
한홍의,우석종,하영걸 ( Hong Ui Han,Suck Jong Woo,Yong Keol Ha ) 한국환경생물학회 1991 환경생물 : 환경생물학회지 Vol.9 No.1
Attempts were made to study microbial community dynamics in the biodegradation of waste cabbage recovered from Kimchi preparations on the soil-bed and waste filtrates obtained from the degraded cabbages. On the soil-bed, Gram positive and Gram negative bacterial communities developed and transformed pH to 4.5, and the concurrent putrefaction of waste cabbages were observed for 6 days. In waste filtrates, pH reached 8.5 by the continuous growth of bacterial communities and thereafter algae started the growth. In bacterial communities, dominant species were Erwinia cypripedii, Lactobadllus farciminis and L. fructosus in the soil-bed, and Erwinia salis, Bacillus coagulans, B. cereus and B. subtilis in the aerated waste filtrates. Dominant algae was identified as Scenedesmus dimorphus. The optimal culture conditions of algae were that illumination density, pH and amount of aeration were 16 Lux, 9, and 4 litre per minute, respectively. Through the algal growth in aerated waste filtrates, phosphorus and nitrogen were removed by about 81% and 60%, respectively.