Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues

Yingjie Sun,Pin Zhang,Hang Zheng,Luna Dong,Lei Tan,Cuiping Song,Xusheng Qiu,Ying Liao,Chunchun Meng,Shengqing Yu,Chan Ding 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1

T-cell internal antigen-1 (TIA-1) has roles in regulating alternative pre-mRNA splicing, mRNA translation, and stress granule (SG) formation in human cells. As an evolutionarily conserved response to environmental stress, SGs have been reported in various species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that it facilitates the assembly of canonical SGs in both human and chicken cells. Overexpression of the chicken prion-related domain (cPRD) of cTIA-1 that bore an N-terminal green fluorescent protein (GFP) tag (pntGFP-cPRD) or Flag tag (pFlag-cPRD) induced the production of typical SGs. However, C-terminal GFP-tagged cPRD induced notably large cytoplasmic granules that were devoid of endogenous G3BP1 and remained stable when exposed to cycloheximide, indicating that these were not typical SGs, and that the pntGFP tag influences cPRD localization. Finally, endogenous cTIA-1 was recruited to SGs in chicken cells and tissues under environmental stress. Taken together, our study provide evidence that cTIA-1 has a role in canonical SG formation in chicken cells and tissues. Our results also indicate that cPRD is necessary for SG aggregation.

An Improved Privacy Preserving Construction for Data Integrity Verification in Cloud Storage

( Yingjie Xia ),( Fubiao Xia ),( Xuejiao Liu ),( Xin Sun ),( Yuncai Liu ),( Yi Ge ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.10

The increasing demand in promoting cloud computing in either business or other areas requires more security of a cloud storage system. Traditional cloud storage systems fail to protect data integrity information (DII), when the interactive messages between the client and the data storage server are sniffed. To protect DII and support public verifiability, we propose a data integrity verification scheme by deploying a designated confirmer signature DCS as a building block. The DCS scheme strikes the balance between public verifiable signatures and zero-knowledge proofs which can address disputes between the cloud storage server and any user, whoever acting as a malicious player during the two-round verification. In addition, our verification scheme remains blockless and stateless, which is important in conducting a secure and efficient cryptosystem. We perform security analysis and performance evaluation on our scheme, and compared with the existing schemes, the results show that our scheme is more secure and efficient.

Development and Analysis of Negative Stiffness Friction Damping Device

Tianwei Sun,Lingyun Peng,Xiaojun Li,Yingjie Kang,Yuke Deng 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.7

A new type of negative stiffness friction damping device (NSFDD) was developed in this study, which can solve the problem of poor energy dissipation of the existing passive negative stiffness damping device. The friction force is generated by squeezing the friction plate with the preloaded nitrogen spring vertically. When the NSFDD is working, the friction plate causes the nitrogen spring to rotate and negative stiffness force and friction force are generated. The results of the performance test show that the NSFDD can achieve negative stiffness in the hysteretic model. Comparing with the existing negative stiffness device, the NSFDD has larger stroke, more stable negative stiffness and better energy dissipation capacity. The finite element software has been used to simulate the low cycle reciprocation of a structure, and the results show that the NSFDD reduces the stiffness of the structure while increasing the damping of the structure. The results of the simulation and analysis of the shock absorption plan of the NSFDD show that the NSFDD reduces the stiffness of the floor, thereby effectively control on the shear force, displacement and acceleration response of the structure.

Towards efficient sharing of encrypted data in cloud-based mobile social network

( Xin Sun ),( Yiyang Yao ),( Yingjie Xia ),( Xuejiao Liu ),( Jian Chen ),( Zhiqiang Wang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.4

Mobile social network is becoming more and more popular with respect to the development and popularity of mobile devices and interpersonal sociality. As the amount of social data increases in a great deal and cloud computing techniques become developed, the architecture of mobile social network is evolved into cloud-based that mobile clients send data to the cloud and make data accessible from clients. The data in the cloud should be stored in a secure fashion to protect user privacy and restrict data sharing defined by users. Ciphertext-policy attribute-based encryption (CP-ABE) is currently considered to be a promising security solution for cloud-based mobile social network to encrypt the sensitive data. However, its ciphertext size and decryption time grow linearly with the attribute numbers in the access structure. In order to reduce the computing overhead held by the mobile devices, in this paper we propose a new Outsourcing decryption and Match-then-decrypt CP-ABE algorithm (OM-CP-ABE) which firstly outsources the computation-intensive bilinear pairing operations to a proxy, and secondly performs the decryption test on the attributes set matching access policy in ciphertexts. The experimental performance assessments show the security strength and efficiency of the proposed solution in terms of computation, communication, and storage. Also, our construction is proven to be replayable choosen-ciphertext attacks (RCCA) secure based on the decisional bilinear Diffie-Hellman (DBDH) assumption in the standard model.

Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene

Yuqiang Cheng,Yingjie Sun,Hengan Wang,Shuduan Shi,Yaxian Yan,Jing Li,Chan Ding,Jianhe Sun 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.1

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 genein duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2’-5’-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx)genes from duck. dTLR5 mRNA was highly expressed in the bursa of Fabricius, spleen, trachea, lung, jejunum, rectum, and skin; moderatelyexpressed in the muscular and glandular tissues, duodenum, ileum, caecum, and pancreas; and minimally expressed in the heart, liver, kidney,and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-B leading to the activation of interleukin-6(IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral moleculesMx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 dayspost-inoculation.

Computational analysis of compressibility effects on cavity dynamics in high-speed water-entry

Chen Chen,Tiezhi Sun,Yingjie Wei,Cong Wang 대한조선학회 2019 International Journal of Naval Architecture and Oc Vol.11 No.1

The objective of this study is to analyze the compressibility effects of multiphase cavitating flow during the water-entry process. For this purpose, the water-entry of a projectile at transonic speed is investigated computationally. A temperature-adjusted Tait equation is used to describe the compressibility effects in water, and air and vapor are treated as ideal gases. First, the computational methodology is validated by comparing the simulation results with the experimental measurements of drag coefficient and the theoretical results of cavity shape. Second, based on the computational methodology, the hydrodynamic characteristics of flow are investigated. After analyzing the cavitating flow in compressible and incompressible fluids, the characteristics under compressible conditions are focused upon. The results show that the compressibility effects play a significant role in the development of cavitation and the pressure inside the cavity. More specifically, the drag coefficient and cavity size tend to be larger in the compressible case than those in the incompressible case. Furthermore, the influence of entry velocities on the hydrodynamic characteristics is investigated to provide an insight into the compressibility effects on cavitating flow. The results show that the drag coefficient and the impact pressure vary with the entry velocity, and the prediction formulas for drag coefficient and impact pressure are established respectively in the present study.