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Kim, Dong Eon,Jang, Mi-Jin,Kim, Young Ran,Lee, Joo-Young,Cho, Eun Byul,Kim, Eunha,Kim, Yeji,Kim, Mi Young,Jeong, Won-il,Kim, Seyun,Han, Yong-Mahn,Lee, Seung-Hyo Elsevier 2017 Toxicology Vol.387 No.-
<P><B>Abstract</B></P> <P>Drug-induced liver injury (DILI) is a leading cause of liver disease and a key safety factor during drug development. In addition to the initiation events of drug-specific hepatotoxicity, dysregulated immune responses have been proposed as major pathological events of DILI. Thus, there is a need for a reliable cell culture model with which to assess drug-induced immune reactions to predict hepatotoxicity for drug development. To this end, stem cell-derived hepatocytes have shown great potentials. Here we report that hepatocyte-like cells derived from human embryonic stem cells (hES-HLCs) can be used to evaluate drug-induced hepatotoxic immunological events. Treatment with acetaminophen significantly elevated the levels of inflammatory cytokines by hES-HLCs. Moreover, three human immune cell lines, Jurkat, THP-1, and NK92MI, were activated when cultured in conditioned medium obtained from acetaminophen-treated hES-HLCs. To further validate, we tested thiazolidinedione (TZD) class, antidiabetic drugs, including troglitazone withdrawn from the market because of severe idiosyncratic drug hepatotoxicity. We found that TZD drug treatment to hES-HLCs resulted in the production of pro-inflammatory cytokines and eventually associated immune cell activation. In summary, our study demonstrates for the first time the potential of hES-HLCs as an <I>in vitro</I> model system for assessment of drug-induced as well as immune-mediated hepatotoxicity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Generation and characterization of hES-HLCs for evaluation of drug-induced immune cell-mediated hepatotoxicity. </LI> <LI> The secretion of inflammatory cytokines is highly enhanced from APAP-treated hES-HLCs. </LI> <LI> Immune cells are activated and produce pro-inflammatory cytokines by conditioned medium from hES-HLCs cultured with APAP. </LI> <LI> Hepatotoxic results in hES-HLCs are consistent with those of primary human hepatocytes. </LI> <LI> Hepatotoxic drugs such as TZD, and non-hepatotoxic drugs such as aspirin and metformin, are also validated with our drug screening system. </LI> </UL> </P>
Deep Switch: 자원이 제약된 기기에서 동적 데이터 변화에 적응하는 모델을 위한 전문화된 경량 신경망 교체 시스템
김학빈(HakBin Kim),김종영(JongYeong Kim),최홍준(HongJun Choi),진영화(YeongHwa Jin),김성웅(SeongWoong Kim),이건호(KeonHo Lee),김현준(HyunJun Kim),한예지(YeJi Han),김다솔(DaSol Kim),김덕환(DeokHwan Kim),최동완(DongWan Choi) 한국정보과학회 2020 한국정보과학회 학술발표논문집 Vol.2020 No.12
Kim Yeji,Kim Jihoo,Kim Won June,Lee Eok Kyun,Choi Insung S. 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.5
3D graph convolutional network (3DGCN) is a graph neural network (GNN) variant that utilizes the 3D bond information on molecules for chemical tasks. In 3DGCN, the pooling operation extracts the molecular features from the atomic features for molecule embedding and dimensionality reduction. In this work, we investigated the pooling effects on the performance of 3DGCN in the classification of protein–ligand binding events, especially when a ligand is rotated with respect to a target protein in the 3D Cartesian coordinate. 3DGCN showed the general ability for recognizing the ligand rotation, and its prediction accuracy was found to be pooling-dependent. For example, 3DGCN with max pooling did not recognize the rotations of known active ligands for human β-secretase 1 faithfully, compared with the other pooling operations (sum, avg, and set2set). This work would contribute to augmented architecture evolution of 3DGCN for the chemical tasks that require the 3D molecular information including chirality.
Kim, Yong-Hoon,Kim, Tae-Hyoung,Lee, Yeji,Kim, Jong-Woong,Kim, Jaekyun,Park, Sung Kyu American Scientific Publishers 2014 Journal of Nanoscience and Nanotechnology Vol.14 No.11
<P>In this paper, we investigate contact-enhanced transparent silver nanowire (Ag NW) network for solution-processed metal-oxide thin-film transistors (TFTs). Mechanical roll pressing was applied to a bar-coated Ag NW film to enhance the inter-nanowire connectivity. As a result, the sheet resistance of the Ag NW film was decreased from 119.5 ψ/square to 92.4 ψ/square, and more stable and enhanced TFT characteristics were achieved when the roll-pressed Ag NW was employed as source/drain electrodes. In addition, a non-acidic wet etching method was developed to pattern the Ag NW electrodes to construct top-contact geometry indium-gallium-zinc oxide TFTs. From the results, it is believed that the mechanical roll pressing and non-acidic wet etching method may be utilized in realizing all solution-based transparent metal-oxide TFTs.</P>
Expression profiles of miRNAs in human embryonic stem cells during hepatocyte differentiation.
Kim, Nury,Kim, Hyemin,Jung, Inkyung,Kim, Yeji,Kim, Dongsup,Han, Yong-Mahn Elsevier 2011 HEPATOLOGY RESEARCH Vol.41 No.2
<P>? Human embryonic stem cells (hESCs) are able to self-renew and differentiate into a variety of cell types. Although miRNAs have emerged as key regulators in the cellular process, a few studies have been reported about behaviors of miRNAs during differentiation of hESCs into a specialized cell type. Here, we demonstrate that different kinds of miRNAs may function in a lineage-specific manner during the differentiation of human embryonic stem cells (hESCs).</P>
Kim, Yeji,Kim, Nury,Park, Sang-Wook,Kim, Hyemin,Park, Han-Jin,Han, Yong-Mahn Korean Society for Stem Cell Research 2017 International journal of stem cells Vol.10 No.1
<P>Although microRNAs have emerged as key regulators in diverse cellular processes, the roles of microRNAs are poorly understood in human embryonic stem cells (hESCs) during differentiation into specialized cell types. In this study, we used a microRNA array with 799 human microRNA probes to examine the expression profiles of microRNAs in hESCs during differentiation into endodermal and mesodermal lineages <I>in vitro</I>. Among the microRNAs analyzed, 7 and 20 microRNAs were enriched in the developmental process of hESCs into mesodermal and endodermal lineages, respectively. In particular, the expression levels of miR-200 family, which is known to regulate the epithelial to mesenchymal transition (EMT), gradually increased in hESCs during differentiation into hepatocytes while they gradually decreased during differentiation into vascular endothelial cells. Downregulation of ZEB1, a direct target of miR-200 family, and E-CADHERIN, a target protein of ZEB1, was observed in hESCs during differentiation into endodermal and mesodermal lineages, respectively. These results indicate that miR-200 family has an important role in determining the cell fate between endodermal and mesodermal lineages from the pluripotent state.</P>
The Occurrence and Flow Analysis of PBDEs in Automobile Shredded Residues in Korea
( Yeji Jang ),( Yuree Kwon ),( Yong-chul Jang ),( Woo-il Kim ),( Ki-heon Kim ),( Dong-gun Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2018 한국폐기물자원순환학회 심포지움 Vol.2018 No.1
Approximately 400 million cars are generated annually worldwide, and about 1 billion cars are being used. A number of existing studies have found that hazardous chemicals such as lead, mercury, hexavalent chromium, PBDEs, and Deca-BDE are detected in end-of-life vehicles (ELVs). In this study, we focused on brominated flame retardants (BFRs) of automobile shredded residue (ASR) generated after recycling of ELVs. PBDEs are often used in textiles and car seat, door trims in automobiles. Such PBDEs are chemicals that may cause endocrine disruption, nervous system toxicity, liver tumors, and thyroid disorders. On the other hand, the law on circulation of waste electrical and electronic equipment and ELVs has been introduced since January 1, 2008 by adopting the EU RoHS, WEEE, and ELV guidelines. According to the criteria for containing harmful substances, the concentration of PBDEs in electrical and electronic products is limited to less than 0.1 W%, but there is no regulation of BFRs in automobiles. Therefore, this study aims to provide basic data on the occurrence of PBDEs in ASR after recycling of ELVs. In this study, 16 ASR samples were collected from eight domestic automobile shredding facilities and quantitative analysis was performed using GC/MS equipment. Material flow analysis(MFA) of ASR was conducted through statistical data and literature survey and expert consultation. Based on the analytical results of BFRs, the average concentration of PBDEs was 1036.6±1421.8 mg/kg with the highest concentration of 5,526.6 mg/kg. Most PBDEs existed as deca-BDE. As a result of MFA, approximately 115 tons-PBDEs were found in ASR as of 2016, and 79 tons (68%) in energy recovery, while about 37 tons (32%) ended up with incineration.