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RISS 인기검색어
- 검색키워드
- 저자 : Yasutomo Iwai (검색결과 5 건)
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The study of essential factors that affect mandibular dental arch forms by cluster analysis
Michiko Nakatsuka,Chizuko Inui-Yamamoto,주성숙,김지연,박영석,Shunji Kumabe,Katsura Ueda,Chun-Ying An,Aiko Morishita,신제원,Yasutomo Iwai 대한구강해부학회 2013 대한구강해부학회지 Vol.34 No.1
To elucidate dent al arch morphological characters in young Japanese adolescents. we studied morphological and dimensional characteristics of the dental arch forms in 396 sets of dental stone study models (age: 18-26: ma le: 257. female: 139) . 53 mandibular arches (male: 29. female: 24) in the diagnostic casts of normal dentition and occ1usion were selected and evaluated. We determined midpoints of the incisal ridge of the incisors (Ih. Ih. 12R & 12d. cusp tips of the canines (CR & Cd. summits of buccal cusps of the premolars (Ph. Ph. P2R & P2d. summits of mesiobuccal cusps of the molars (Mh. Mh. M2R & M2d and the midpoint A of line (Ih-Ih) . Point B was defined as the intersection of the vertical line from point A with the line (M2R-M2L). Point E was the intersection of (A-B) with (CR-Cd. The midpoint of line (M2R-M2L : original line) is defined as point O. We examined the mandibular arch forms by estimation of length. angles and the transition of anterior and buccal segments which were represented by items developed from the reference points and lines . The obtained data were studied using a hierarchical c1uster analytic approach: the factor loading of principal components in the c1usters O.e.. pt’ 2nd and 3rd c1usters) were further evaluated By the analyses. we observed significant differences in each c1uster.especially of the arch length. protrusion of canines. degree of curvature of anterior teeth segment and the degree of roundness of the mandibular dental arches. Arch length of the 3rd cluster was longer than that of the other clusters . In addition. we noticed that width differences of the dental arch and anterior segment were useful to distingui sh between the 1st and 2nd clusters . The study concludes that the mandibular dental arch form consisted of essential components of arch length. arch width and the curvature of anterior teeth. Besides. we can effectively divide the mandibular arch form into clusters by dental arch length and width.
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Evaluation of MAPK pathway activation in brainstem induced by the masseter muscle inflammation
Michiko Nakatsuka,Shunji Kumabe,Shoko Gamoh,Hironori Akiyama,주성숙,김지연,Katsura Ueda,Yoshifumi Matsuda,Kimishige Shimizutani,신제원,Yasutomo Iwai 대한구강해부학회 2014 대한구강해부학회지 Vol.35 No.1
To evaluate the inflammatory hyperalgesia induced by noxious stimulation of the masticatory muscles, we performed an immunohistochemical study on the expressions of phosphorylated-p38 (p-p38) mitogen activated-protein kinase (MAPK) and the distribution of activated microglia in the trigeminal subnucleus caudalis (Vc). The left masseter muscle (LMM) of Sprague Dawley rats (male, 250 g, n=60) was stimulated in the following methods: 1) L-L group (control); the LMM was injected with lipopolysaccharide (LPS, 2 μg/kg, 100μl) on the 1st day of the experiment. On day 2, the same site was injected with the LPS again. 2) L-S6 group (experimental); the LMM was injected with LPS (2 μg/kg, 100μl) on the 1st day of the experiment. On day 2, the same site was injected with 6 % sodium chloride solution (S6, 100 μl, 5 times per 90 min). Rats were allowed to survive for 1 day, 7 days or 14 days after the last injection. The brainstems were dissected and cut with a cryostat (at 30 μm thickness). These specimens were investigated with anti-TNFα (masseter muscle), the bradykinin receptor B2 (BKRB2, masseter muscle), anti-p-p38 MAPK (brainstem) and anti-Iba1 (ionized calcium-binding adapter molecule 1: Iba1, a marker for microglial activity; brainstem) enzyme-labeled antibody method. The specimens were observed and evaluated using a light microscope(LM) mounted with an Olympus FX380 3CCD digital camera system connected with a FLvFs software (Flovel Image Filling System, Tokyo, Japan). In both groups, the TNF-α and the BKRB2-immunoreactive (IR) cells were observed until 7 days after stimulation. In the experimental group, the LM histology indicates that p-p38 MAPK and Iba1-IR cells were particularly localized in the left Vc until 14 days after stimulation. In the experimental group, 7 days or 14 days after nociception, the p-p38 MAPK-IR cells were recognized in the contralateral and ipsilateral in the Vc. The results suggest that the prolonged MAPK activity in the Vc is related to central sensitization in chronic pain of the masseter muscle.
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Aikawa, Fumiko,Nakatsuka, Michiko,Kumabe, Shunji,Jue, Seong-Suk,Hayashi, Hiroyuki,Shin, Je-Won,Iwai, Yasutomo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.3
Tissue stem cells are used for the regenerative medicine. In previous study we observed hard tissue formation of human dental pulp-derived cells using alginate scaffold. In this study, we explore the ability to differentiate of the 13th passage cells with glycerol 2-phosphate disodium salt hydrate (${\beta}-GP$) which accelerate calcification. Reverse transcriptase Polymerase Chain Reaction (RT-PCR), transplants using alginate scaffold and histological examination were performed. We observed the expression of DSPP mRNA on day 10 cultured cells with ${\beta}-GP$. In conclusion, the 13th passage cells still have an ability to differentiate into odontoblast-like cells and alginate supports the differentiation of cultured cells in the transplants.
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Fourier Analysis of Maxillary Dental Arch Forms
Michiko Nakatsuka(나카츠카 미치코),Tohru Tsujibayashi(쯔지바야시 토오루),Shunji Kumabe(쿠마베 슌지),Seong-Suk Jue(주성숙),Je-Won Shin(신제원),Yasutomo Iwai-Liao(이와이 야스토모) 대한체질인류학회 2008 해부·생물인류학 (Anat Biol Anthropol) Vol.21 No.2
사람 위이틀활의 기준점, 기준선, 기준각을 이용한 연구를 통하여 전치부의 형태와 이틀활의 형태 사이에 유의한 관계가 있음을 알게 되었다. 이에 사람 위이틀활 형태 결정에 영향을 미치는 필수요소들 사이의 관계를 밝히기 위하여 사람 위이틀활 모델 62개의 형태를 푸리에 급수를 사용하여 비교 검토하였다. 푸리에 급수의 상수 및 제 1 주파수의 진폭은 각 형태간에 통계적으로 유의성 있는 차이를 관찰할 수 없었지만 제 2 주파수에서 제 4 주파수까지의 진폭에서는 각 형태간에 유의성 있는 차이를 관찰할 수 있었다. 또한 상수는 앞니부분의 폭경, 이틀활의 폭경 및 이틀활의 길이와 각각 정비례 관계를 보였다. 이상의 결과로 보아, 사람 위이틀활의 형태는 앞니부분으로부터 어금니부분으로의 이행부의 형태가 관여하고 있는 것으로 생각된다. Our previous principal component analysis conducting on reference points, lines and angles, and a vector-developed polar coordinate system has elucidated that the components of eigenvectors had positive relationships in the curvature of anterior teeth segment, between the protrusion of canines and degree of arch roundness, and in the length-to-width ratio of 62 maxillary dentitions, which were preliminarily classified with reference to the conventional Thompson’s morphological descriptions for dental arch forms. In the present study on morphological characters of the maxillary dentitions, we conducted a Fourier analysis on the previously obtained data. We observed that the amplitude of 2<SUP>nd</SUP>, 3<SUP>rd</SUP> and 4<SUP>th</SUP> Fourier harmonics were closely correlated with the length-to-width ratio, curvature of the anterior teeth segment, and the curvilinear contour of maxillary dental arches. In addition, the relationships between previously estimated data and the constant value and the amplitude of the Fourier series were examined by analysis of correlation coefficients(p<0.01). The results of the present study suggest that the morphology of maxillary dentitions consists of three essentials-the length-to-width ratio, the curvature of anterior teeth and the curvilinear contour of dental arches.
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