Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Yan‐xin Zhang,Wei Hua,Lin‐han Wang,Zhuo Che,Xiu‐rong Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.3

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.

豪雨時 浮遊砂의 最適 SAMPLING 間隔에 關한 硏究(渭川 流域을 中心으로)

趙廷錫,韓良洙,朴相圭 대구대학교 과학기술연구소 1996 科學技術硏究 Vol.3 No.1

This study calculated the optimal sampling interval of suspended sediment on storm in terms of Kalman filter method after collected data the discharge and suspended sediment of Museong place, the downstream of the Wi stream basin that is one of the IHP representative basin the Nakdong river water systems. We'll have to study more specifically about the various elements closely related to the discharge with the reasonable and correct standard of the error variance in the future and we finds out the suspended sediment optimal sampling intervals in other basins it will be applied by the characteristic of them.

객체 관계형 데이타베이스 관리 시스템을 이용한 디지털 앨범의 설계 및 구현

이준서,장염승,한기준 건국대학교 산업기술연구원 2000 건국기술연구논문지 Vol.25 No.-

As the storage capacity of a computer system is rapidly increasing and the cost of computer hardware is decreasing continuously, users can easily manage multimedia data on the personal computer. Especially, various image view programs are widely used to manage image files at the present time. However, since most of these programs do not use a database management system(DBMS), it is very difficult for users to use them efficiently. In this paper, we designed and implemented a Digital Album by using an object-relational database management system, called PARODY(Persistent, Almost, Relational Object Database sYstem) that implements a persistent object database with a relatively simple interface to its C++ class library. Since PARODY is used as a data storage manager of the Digital Album, users can manage(i.e., insert, update, and delete) an image database and search for the required image data from the image database easily. In addition, we use OWL(Object Windows Library) of Borland C++ to implement the user interface of the Digital Album efficiently.

Differences in Epidermal Growth Factor Receptor Gene Mutations and Relationship with Clinicopathological Features in NSCLC Between Uygur and Han Ethnic Groups

Zhang, Yan,Wang, Qiang,Han, Zhi-Gang,Shan, Li Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: To investigate differences in mutations of epidermal growth factor receptor (EGFR) gene and relationships with clinicopathological features in patients with non-small cell lung cancer (NSCLC) between Uygur and Han ethnic groups. Methods: The Scorpions amplification refractory mutation system (Scorpions ARMS) was used to measure mutations in exons 18, 19, 20 and 21 of the EGFR gene in paraffin-embedded tumor tissue from NSCLC cases, and statistical analysis was performed to investigate links with clinicopathological features in different histological types of NSCLC. Results: Results from ARMS testing showed EGFR mutations in tumor tissues from six (6) of 50 NSCLC patients of Uygur ethnic group, with a positive rate of 12.0%; four of them (4) had exon 19 deletion in EGFR, and two (2) had L858R point mutation in exon 21 of EGFR. Statistically significant difference was noted in EGFR genetic mutation between adenocarcinoma and non-adenocarcinoma (P < 0.05), but no differences with gender, age group, smoking status, or stage (P > 0.05). EGFR mutations were detected in tumor tissues from 27 of 49 NSCLC patients of Han ethnic group, with a positive rate of 55.1%; 19 of them had exon 19 deletions, seven (7) had L858R point mutations in exon 21 of EGFR and one (1) had mutations in both exon 18 G719X and exon 20 T790M of EGFR. Statistically significant differences were noted in EGFR genetic mutations between genders and between adenocarcinoma and non-adenocarcinoma (P<0.05), but not with age group, smoking status, or stage (P > 0.05). Conclusion: Statistically significant differences were noted in the positive rates of EGFR genetic mutations in NSCLC patients between Uygur and Han ethnic groups, with lower positive rates for the Uygur cases.

Genetic Variants at 6p21.1 and 7p15.3 Identified by GWASs of Multiple Cancers and Ovarian Cancer Risk: a Case-control Study in Han Chinese Women

Li, Da-Ke,Han, Jing,Liu, Ji-Bin,Jin, Guang-Fu,Qu, Jun-Wei,Zhu, Meng,Wang, Yan-Ru,Jiang, Jie,Ma, Hong-Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

A recent study summarized several published genome-wide association studies (GWASs) of cancer and reported two pleiotropic loci at 6p21.1 and 7p15.3 contributing to multiple cancers including lung cancer, noncardia gastric cancer (NCGC), and esophageal squamous-cell carcinoma (ESCC) in Han Chinese. However, it is not known whether such genetic variants have similar effects on the risk of gynecologic cancers, such as ovarian cancer. Hence, we explored associations between genetic variants in 6p21.1 and 7p15.3 and ovarian cancer risk in Han Chinese women. We performed an independent case-control study by genotyping the two loci (rs2494938 A > G at 6p21.1 and rs2285947 A > G at 7p15.3) in a total of 377 ovarian cancer cases and 1,034 cancer-free controls using TaqMan allelic discrimination assay. We found that rs2285947 at 7p15.3 was significantly associated with risk of ovarian cancer with per allele odds ratio (OR) of 1.33 [95% confidence interval (CI): 1.08-1.64, P=0.008]. However, no significant association was observed between rs2494938 and ovarian cancer risk. Our results showed that rs2285947 at 7p15.3 may also contribute to the development of ovarian cancer in Han Chinese women, further suggesting pleiotropy of 7p15.3 in multiple cancers.

The Influence of Nozzles on Bottomhole Flow Field of Dual-diameter PDC Bit

Yan Yan,Zhichuan Guan,Cai Yang,Han Yan 한국유체기계학회 2020 International journal of fluid machinery and syste Vol.13 No.1

In order to improve cleaning efficiency of dual-diameter PDC bit, the total flow area, inclination angle and azimuth angle of bit’s pilot and reaming nozzles were analyzed. By numerical simulation, the velocity, pressure and particle distribution of bottomhole are obtained. The results show that low pressure area of pilot downhole decreases while low pressure of reamer downhole increases with the increase of total flow area. The mud velocity of central bottomhole has the negative relationship with inclination angle and positive relationship with azimuth angle. Best cleaning efficiency can be achieved when total flow area is 12 ㎠, inclination angles of pilot and reamer are respectively 15° and 25°, and azimuth angles of pilot and reamer are respectively 60° and 30°.

Sensitive-resisting Relation Social Network Privacy Protection Model

Han Yan 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.8

The existing social network privacy protection method mostly aims at the individuals of the social network, which cannot protect effectively the sensitive relations in the social network. Therefore, this paper proposes a new personalized K_L model. This model requires each sensitive relation with the sensitive relational point have l at least, and also the point with the same requirement has k at least. Thus, the attack has been resisted during the protection of the sensitive relations. Through seeking the most figure of merit sequence and considering individual sensitive attribute, the L-diversity method is applied so as to guarantee the least side and reduce the anonymous cost. Through the data set experiment, this paper proposes new personalized model K_L, which has the high anonymous quality and can effectively protect user's privacy in the social network.

A Novel Modulation Strategy for Diode-assisted Buck-boost VSI with Maximum Voltage Gain and Minimum Switching Frequency

Yan Zhang,Xiaolong Ma,Jinjun Liu,Zhuo Dong,Shah Zaman,Jiuqiang Han 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Diode-assisted buck-boost voltage source inverter (VSI) boosts the dc source voltage by introducing a diode-capacitor network. With capacitive charging in parallel and discharging in series, it extends voltage regulation range and avoids extreme boost duty ratio. As for the unique structure, various pulse width modulation (PWM) strategies are provided with regarding to the chopped intermediate dc-link voltage. In order to maximize voltage gain, as well as to achieve the increased efficiency, this paper proposed a novel modulation strategy by reducing the switching frequency of power devices in the inverter bridge. The operation principle and relationship of switching device voltage stress versus voltage gain are analyzed in detail. Simulation and experiments are implemented to verify the theoretical analysis. Compared with existing modulation strategies, diode-assisted buck? boost VSI with new modulation scheme demonstrates good performances: less power device requirement and higher efficiency in high voltage gain application. Owing to improved performance, it is a more competitive topology for wide range dc/ac voltage regulation in renewable energy application, especially for ac output with relatively high fundamental frequency. Furthermore, the idea of proposed modulation scheme can also be extended to the control of other quasi two-stage inverter.

The Protective Role of TLR3 and TLR9 Ligands in Human Pharyngeal Epithelial Cells Infected with Influenza A Virus

Yan Han,Ming-yu Xu,Nan Sun,Dan-hong Liu,Zhi-jian Bo 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3

In this study we aim to extensively investigate the anti-influenza virus immune responses in humanpharyngeal epithelial cell line (Hep-2) and evaluate the protective role of Toll-like receptor (TLR)ligands in seasonal influenza A H1N1 (sH1N1) infections in vitro. We first investigated the expressionof the TLRs and cytokines genes in resting and sH1N1 infected Hep-2 cells. Clear expressions of TLR3,TLR9, interleukin (IL)-6, tumour necrosis factor (TNF)-α and interferon (IFN)-β were detected inresting Hep-2 cells. After sH1N1 infection, a ten-fold of TLR3 and TLR9 were elicited. Concomitantwith the TLRs activation, transcriptional expression of IL-6, TNF-α and IFN-β were significantlyinduced in sH1N1-infected cells. Pre-treatment of cells with poly I:C (an analog of viral double-strandedRNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) resulted in a strong reductionof viral and cytokines mRNA expression. The results presented indicated the innate immune responseactivation in Hep-2 cells and affirm the antiviral role of Poly I:C and CpG-ODN in the protectionagainst seasonal influenza A viruses.

New Gene Cluster from Thermophile Bacillus fordii MH602 for Conversion of DL-5-Substituted Hydantoins to L-Amino Acids

Yan Zhen Mei,Yong Min Wan,Bing Fang He,Han Jie Ying,Ping Kai Ouyang 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.12