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- 저자 : Yanjie Zheng (검색결과 3 건)
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Zheng Lin Zhao,XiuDong Jin,Yi Yan Wu,Xu Dong Yang,YanJi Xu,James ZhongJian Jiang,김상찬,이봉효,양재하,조용걸 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.5
In a previous study, acupuncture at acupoint HT7 attenuated ethanol withdrawal-induced anxiety-like behavior in rats by normalizing amygdaloid catecholamines. In the present study, the involvement of amygdaloid corticotropin-releasing factor (CRF) in the anxiolytic effect of acupuncture was investigated during ethanol withdrawal. Rats were intraperitoneally treated with 3 g /kg/day of ethanol for 28 days, and the CRF mRNA levels in the central nucleus of the amygdala (CEA) were measured by using a RT-PCR analysis 72 hours after the last dose of ethanol. During ethanol withdrawal, the rats were bilaterally treated with acupuncture at acupoints HT7, PC6 or at a non-acupoint (Tail) for one min/day for three days. Also, rats were bilaterally injected with CRF into the CEA five minutes after the third acupuncture treatment , after which followed by the elevated-plus maze (EPM) test and the plasma corticosterone radioimmunoassay (RIA) were administered. The RT-PCR analysis showed a significant increase in the amygdaloid CRF mRNA levels in the ethanol-withdrawn rats compared with both the saline-treated rats and the rats treated with acupuncture at HT7, but neither acupuncture at PC6 nor acupuncture at a non-acupoint significantly inhibited the increased mRNA expression. The EPM test and the RIA also showed that the post-acupuncture infusion of CRF greatly reduced the anxiolytic effect of acupuncture at HT7. These results suggest that during ethanol withdrawal, the anxiolytic effect of acupuncture may be mediated through the modulation of amydaloid CRF during ethanol withdrawal.
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Differentiation potential of neural stem cells derived from fetal sheep
Qian Li,Minghai Zhang,Wei Jun Guan,Shuang Zhang,Yanjie Zheng,Hebao Wen,Xiao Han 한국통합생물학회 2017 Animal cells and systems Vol.21 No.4
Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal Ovis aries. Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Moreover, a reverse transcription–polymerase chain reaction assay was used to biologically characterize the cell line. NSCs were induced to differentiate into neurogenic cells in vitro. They expressed MAP2, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). In this study, we successfully isolated and cultivated NSCs from the hippocampal tissue of fetal sheep. NSCs not only displayed a self-renewal capacity but also had the potential to differentiate into neurons and glial cells. This study provided valuable experimental data for NSC transplant research.
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Shaohuang Weng,Feng Feng,Honghui Guo,Namei Wu,Yanjie Zheng,Zhen Lin,Xinhua Lin 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.3
Gold nanoparticles (Au NPs)-based colorimetric assay for the highly sensitive and selective detection of glucose has been conducted. The sensing, which was based on the in situ growth of Au NPs in the presence of glucose, was developed with the solution changing from colorless to red. This change could be observed with the naked eye or a ultraviolet–visible (UV–vis) spectrophotometer. Moreover, adding trace amounts of Tween-20 kept Au NPs well spaced. Under the optimal assay conditions, the absorbance of Au NPs at 550 nm was proportional to glucose concentration within 400–1800 μM with a linear regression equation, and the detection limit was 6.28 μM. Some common interfering compounds in beverages, including other sugars, food additives, and amino acids, did not affect glucose sensing. Therefore, the proposed Au NP-based colorimetric assay could be successfully used to determine glucose in beverages.
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