Central role of autophagic UVRAG in melanogenesis and the suntan response

Yang, Yongfei,Jang, Gyu-beom,Yang, Xuanjun,Wang, Qiaoxiu,He, Shanshan,Li, Shun,Quach, Christine,Zhao, Shihui,Li, Fan,Yuan, Zengqiang,Lee, Hye-Ra,Zhong, Hanbing,Liang, Chengyu National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.33

<P>UV-induced cell pigmentation represents an important mechanism against skin cancers. Sun-exposed skin secretes alpha-MSH, which induces the lineage-specific transcriptional factor MITF and activates melanogenesis in melanocytes. Here, we show that the autophagic tumor suppressor UVRAG plays an integral role in melanogenesis by interaction with the biogenesis of lysosome-related organelles complex 1 (BLOC-1). This interaction is required for BLOC-1 stability and for BLOC-1-mediated cargo sorting and delivery to melanosomes. Absence of UVRAG dispersed BLOC-1 distribution and activity, resulting in impaired melanogenesis in vitro and defective melanocyte development in zebrafish in vivo. Furthermore, our results establish UVRAG as an important effector for melanocytes' response to alpha-MSH signaling as a direct target of MITF and reveal the molecular basis underlying the association between oncogenic BRAF and compromised UV protection in melanoma.</P>

Comparative analysis of metabolites and in vitro hypoglycemic activity of Taiwanofungus camphoratus cultured using various methods

Ming YongFei,Li Yin,Chu JianZhi,Zhou XiaoShuang,Huang YuXuan,Yang ShuDe,Mu YueJun,Wang Lin,Zhang Rui,Cheng XianHao 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Taiwanofungus camphoratus has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, T. camphoratus is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. T. camphoratus produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of T. camphoratus produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of T. camphoratus extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by T. camphoratus under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using T. camphoratus extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by T. camphoratus and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of T. camphoratus.

A Distributed Privacy-Utility Tradeoff Method Using Distributed Lossy Source Coding with Side Information

( Yonghao Gu ),( Yongfei Wang ),( Zhen Yang ),( Yimu Gao ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.5

In the age of big data, distributed data providers need to ensure the privacy, while data analysts need to mine the value of data. Therefore, how to find the privacy-utility tradeoff has become a research hotspot. Besides, the adversary may have the background knowledge of the data source. Therefore, it is significant to solve the privacy-utility tradeoff problem in the distributed environment with side information. This paper proposes a distributed privacy-utility tradeoff method using distributed lossy source coding with side information, and quantitatively gives the privacy-utility tradeoff region and Rate-Distortion-Leakage region. Four results are shown in the simulation analysis. The first result is that both the source rate and the privacy leakage decrease with the increase of source distortion. The second result is that the finer relevance between the public data and private data of source, the finer perturbation of source needed to get the same privacy protection. The third result is that the greater the variance of the data source, the slighter distortion is chosen to ensure more data utility. The fourth result is that under the same privacy restriction, the slighter the variance of the side information, the less distortion of data source is chosen to ensure more data utility. Finally, the provided method is compared with current ones from five aspects to show the advantage of our method.

Research and Implementation of U-Learning System Based on Experience API

Xinghua Sun,Yongfei Ye,Jie Yang,Li Hao,Lihua Ding,Haomin Song 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.3

Experience API provides a learner-centered model for learning data collection and learning process recording. In particular, it can record learning data from multiple data sources. Therefore, Experience API provides verygood support for ubiquitous learning. In this paper, we put forward the architecture of ubiquitous learningsystem and the method of reading the learning record from the ubiquitous learning system. We analyze students’learning behavior from two aspects: horizontal and vertical, and give the analysis results. The system canprovide personalized suggestions for learners according to the results of learning analysis. According to thefeedback from learners, we can see that this u-learning system can greatly improve learning interest and qualityof learners.

Numerical calculation and optimization designs in engine cooling water pump

Li Wei,Wang Chuan,Shi Weidong,Zhao Xiaofan,Yang Yongfei,Pei Bing 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.5

The cavitation damage in the Engine cooling water pump (ECWP) is the key factor that shortens the lifespan of automobile cooling systems, and causes vibration and noise. To improve cavitation performance and external characteristics of ECWP, three optimized models were designed on the basis of WP7 diesel ECWP. The whole flow fields in three models and the prototype pump were numerically simulated, employing the time averaged Navier-Stokes equation, the standard k-ε turbulent model and Zwart-Gerber-Belamri multiphase flow model by ANSYS-CFX software. Pressure distribution, turbulent kinetic energy distribution, bubble volume fraction distribution, external characteristics and cavitation performance of the prototype pump and optimized models were compared and analyzed. The results show that the external characteristics and cavitation performance of the optimized models are significantly better than that in the prototype pump. Through decreasing the inlet blade angle and wrap angle, extending the blade to inlets and extending a certain inclination in the blade, shock loss in blade inlet was reduced and so, the performance of pump will be improved. The flow condition at blade inlet will also be improved greatly, which in turn improves cavitation performance. When reducing the quantity of blades, the excretion coefficient will drop, flow area of blade inlet becomes bigger, but head has a little drop. And the pump optimized by reducing the quantity of blades has the optimal cavitation performance among three optimized models. With the decrease of impeller diameter, the absolute pressure in the critical cavitation point becomes bigger, the inlet bubble volume fraction at the same absolute pressure increases while cavitation performance gets worse. The obtained numerical results were compared with the experimental ones, and the outcome showed the same tendency between the two along with acceptable error.