http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
실험적 뇌지주막하출혈 토끼에서의 Malonate 농도 측정
김재중,하영수,백승렬,장정순,김유삼 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.10
Malonate is regarded as a reversible competitive inhibitor of succinate dehydrogenase and malate transport in the Krebs cycle and showed neurotoxicity by persistent NMDA receptor activation due to inhibition of ATP production and glutamate utilization. However, little was known about its biological effects and the range of normal concentration of malonate in central nervous system. In order to understand the relationship between malonate and vasospsasm, malonate concentrations in rabbit model of experimental subarachnoid hemorrhage were measured at 0, 4th, and 7th day following SAH in serum, cerebrospinal fluid, and urine using malonyl-CoA synthetase. The results were as follows : 1) Malonate level is increased significantly in serum at the 4th day after subarachnoid hemorrhage that followed by vasospasm(p<0.01). 2) CSF malonate concentration tends to increase at post-SAH 7th day but statistically not significant. 3) The change of urine malonate concentration is not significant. These results suggest that early increase of serum malonate level is significant because clinically important vasospam begin from the fourth through the seven day after hemorrhage. The increased level of serum malonate at this time is due to impairment of cellular metabolism following delayed cerebral ischemia and may influence to development of vasospasm. In conclusion, the measurement of serum malonate concentration after subarachnoid hemorrhage is one of possible candidates for the early diagnosis of vasospasm.
( Young Gu Kim ),( Young Kyeong Seo ),( Yu Seong Min ),( Han Baek Son ),( Ki Tak Bae ),( Eun Uk Jung ),( Sang Heon Lee ),( Jung Sik Choi ),( Sam Ryong Jee ),( Youn Jae Lee ),( Sang Young Seol ),( Sung 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Hepatitis C virus infection frequently causes chronic liver disease leading to cirrhosis and hepatocellular carcinoma (HCC). There are few studies addressing the effi cacy of treatmenet with full-dose peginterferon-alpha plus weightadjusted RBV dose in a patient population with compensated cirrhosis. We compared long term outcomes and adverse event profiles between cirrhotic patients with or without antiviral treatments. Methods: This is a single centered, retrospective study of patients with HCV-related cirrhosis who, since 2002, had received treatment in a academic hospital in Busan, Korea. Diagnosis of cirrhosis was biopsy proven in 6 cases (5.66%) and the remaining 100 cases (94.34%) met at least one of the following criteria: platelet count < 100 × 109 per liter and presence of esophageal varices on endoscopy or presence of radiologic finding suggestive of liver cirrhosis. A total of 57 patients were treated with PEG-IFN alpha-2a/ribavirin or PEG-IFN alpha-2b/ribavirin and the treatment response was assessed based on the sustained virologic response (SVR). Overall, there were 28 patients (49.1%) who achieved SVR and 12 patients (21.1%) who did not achieve SVR. We compared several variables such as overall survival, event free survival, liver-related death and events rate of decompensation between cirrhotic patients with or without antiviral treatments. Results: Overall survival in patients with SVR was 93.1% vs. 61.3% in control group (P=0.005) and Overall survival in patients without SVR was 85.7% vs. 61.3% in control group (P=0.390). Event-free survival was 89.3% in patients with SVR vs. 32.3% in control group (P<0.001) and Event-free survival was 71.4 % in patients without SVR vs. 32.3% in control group (P=0.089). Liver-related deaths was 3.57 % in patients with SVR vs. 29.0 % in control group (P=0.013) and Liver-related deaths was 14.3 % in patients without SVR vs. 29.0 % in control group (P=0.018). During the study, 14 patients died (11 in control group and 3 in treatment group). In control group, the cause of deaths are liver-related problems such as HCC progression( 5) ,HRS(3), SBP(1) and non-liver-related problems such as pneumonia(1) and pleural effusion(1) In treatment group, the cause of deaths are liver-related problems such as HCC progression (2) and non-liver-related problems such as cerebral hemorrhage (1) In analysis of events of decompensation, only the incidences of CPT score >1 (P=0.022) was significantly lower in the anti-viral treatment group with SVR. But, The incidence of hepatocellular carcinoma(P=0.200) and variceal hemorrhage(P=1.000) were not statistically associated with anti-viral treatment. Conclusions: Treatment with full-dose peg interferon-alpha plus weight-adjusted RBV dose in a patient population with compensated cirrhosis reduces the likelihood of clinical decompensation and improves event free survival.
Seo, Young Sam,Kim, Eun Yu,Kim, Woo Taek Oxford University Press 2011 Journal of experimental botany Vol.62 No.15
<P>Lipid-derived molecules produced by acylhydrolases play important roles in the regulation of diverse cellular functions in plants. In <I>Arabidopsis</I>, the DAD1-like phospholipase A1 family consists of 12 members, all of which possess a lipase 3 domain. In this study, the biochemical and cellular functions of AtDLAH, an <I>Arabidopsis thaliana</I> DAD1-like acylhydrolase, were examined. Bacterially expressed AtDLAH contained phospholipase A1 activity for catalysing the hydrolysis of phospholipids at the <I>sn</I>-1 position. However, AtDLAH displayed an even stronger preference for 1-lysophosphatidylcholine, 1-monodiacylglycerol, and phosphatidic acid, suggesting that AtDLAH is a <I>sn</I>-1-specific acylhydrolase. The <I>AtDLAH</I> gene was highly expressed in young seedlings, and its encoded protein was exclusively localized to the mitochondria. <I>AtDLAH</I>-overexpressing transgenic seeds (<I>35S:AtDLAH</I>) were markedly tolerant to accelerated-ageing treatment and thus had higher germination percentages than wild-type seeds. In contrast, the <I>atdlah</I> loss-of-function knockout mutant seeds were hypersusceptible to accelerated-ageing conditions. The <I>35S:AtDLAH</I> seeds, as opposed to the <I>atdlah</I> seeds, exhibited a dark red staining pattern following tetrazolium treatment under both normal and accelerated-ageing conditions, suggesting that AtDLAH expression is positively correlated with seed viability. The enhanced viability of <I>35S:AtDLAH</I> seeds was accompanied by more densely populated epidermal cells, lower levels of accumulated lipid hydroperoxides, and higher levels of polar lipids as compared with wild-type and <I>atdlah</I> mutant seeds. These results suggest that AtDLAH, a mitochondrial-localized <I>sn</I>-1-specific acylhydrolase, plays an important role in <I>Arabidopsis</I> seed viability.</P>
Seo, Young Sam,Choi, Jun Young,Kim, Soo Jin,Kim, Eun Yu,Shin, Jeong Sheop,Kim, Woo Taek Springer 2012 Plant cell reports Vol.31 No.9
<P>CaRma1H1, an endoplasmic reticulum (ER)-localized hot pepper really interesting new genes (RING) E3 Ub ligase, was previously reported to be a positive regulator of drought stress responses. To address the possibility that CaRma1H1 can be used to improve tolerance to abiotic stress in crop plants, CaRma1H1 was constitutively expressed in transgenic tomato (Solanum lycopersicum) plants. CaRma1H1-overexpressing tomato plants (35S:CaRma1H1) exhibited greatly enhanced tolerance to high-salinity treatments compared with wild-type plants. Leaf chlorophyll and proline contents in CaRma1H1 overexpressors were 4.3- to 8.5-fold and 1.2- to 1.5-fold higher, respectively, than in wild-type plants after 300 mM NaCl treatment. Transgenic cotyledons developed and their roots elongated in the presence of NaCl up to 200 mM. In addition, 35S:CaRma1H1 lines were markedly more tolerant to severe drought stress than were wild-type plants. Detached leaves of CaRma1H1 overexpressors preserved water more efficiently than did wild-type leaves during a rapid dehydration process. The ER chaperone genes LePDIL1, LeBIP1, and LeCNX1 were markedly up-regulated in 35S:CaRma1H1 tomatoes compared with wild-type plants. Therefore, overexpression of CaRma1H1 may enhance tomato plant ER responses to drought stress by effectively removing nonfunctional ubiquitinated proteins. Collectively, constitutive expression of CaRma1H1 in tomatoes conferrred strongly enhanced tolerance to salt- and water-stress. This raises the possibility that CaRma1H1 may be useful for developing abiotic stress-tolerant tomato plants. Key message CaRma1H1 increases drought tolerance in transgenic tomato plants.</P>
Kim, Yu Sam,Bahk, Young Yil,Lee, Gha Young 생화학분자생물학회 1969 BMB Reports Vol.35 No.2
Malonyl-CoA decarboxylase (E.C.4.1.1.9) catalyses the conversion of malonyl-CoA to acetyl-CoA. Although the metabolic mle of this enzyme has not been fully defined, it has been reported that its deficiency is associated with mild mental retardation, seizures, hypotonia, cadiomyopathy, developmental delay, vomiting, hypoglycemia, metabolic acidosis, and malonic aciduria. Here, we isolated a cDNA clone for malonyl CoA decarboxylase from a rat brain cDNA library, expressed it in E. coli, and characterized its biochemical properties. The full-length cDNA contained a single open-reading frame that encoded 491 amino acid residues with a calculated molecular weight of 54, 762 Da. Its deduced amino acid sequence revealed a 65.6% identity to that from the goose uropigial gland. The sequence of the first 38 amino acids represents a putative mitochondrial targeting sequence, and the last 3 amino acid sequences (SKL) represent peroxisomal targeting anes. The expression of malanyl CoA decarboxylase was observed over a wide range of tissues as a single transcript of 2.0 kb in size. The recombinant protein that was expressed in E. coli was used to characterize the biochemical properties, which showed a typical Michaelis-Menten substrate saturation pattern. The K_m and V_(max) were calculated to be 68 μM and 42.6 μmol/min/mg, respectively.