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( Xing Ai Gao ),( Wan Taek Ju ),( Yong Feng Zhang ),( Rong De Jin ),( Si Yan Liu ),( Ro Dong Park ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.1
A chitosanase-producing bacterium, Bacillus cereus D-11, was isolated from an environmental soil sample. The optimal culture condition for chitosanase production by B. cereus D-11 was 3 days of cultivation at 30 o C in a medium composed of 0.5% inoculation concentration (1.4×10 8 CFU/mL), 0.7% colloidal chitosan, 1% yeast extract and 1% NaCl at initial pH 7.0. The highest activity achieved was 7.8 U/mL. The crude enzyme preparation from Bacillus cereus D-11 degraded the chitooligomers pentamer, hexamer, and heptamer into (GlcN)2-4, but not degraded the chitooligomers less than (GlcN)4. These results indicate that B. cereus D-11 chitosanase cleaves the oligomeric chains in the endo-splitting manner and the catalytic domain of D-11 chitosanase recognizes and needs at least 5 glucosamine units for hydrolytic catalysis of the glycosidic linkages.
Preparation of Chitooligosaccharides from Chitosan using Crude Enzyme of Bacillus cereus D-11
( Xing Ai Gao ),( Yong Feng Zhang ),( Ro Dong Park ),( Xiao Huang ),( Xin Ying Zhao ),( Jiao Xie ),( Rong De Jin ) 한국응용생명화학회 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.1
In order to enzymatically produce chitooligosaccharide using the crude enzyme preparation from Bacillus cereus D-11, we first studied the optimal reaction conditions. It was found that the optimal temperature for hydrolysis of chitosan was 55oC. The ratio of enzyme/substrate should not be lower than 0.13 U/mg in the reaction mixture. The enzyme activity was stable below 50oC. The products of enzymatic reaction were analyzed by both thin layer chromatography and high performance liquid chromatography. Under the appropriate condition, chitosan was hydrolyzed using the enzyme preparation. The resulting chitooligosaccharides were purified and separated by Dowex (H+) ion exchange chromatography. From 4 g soluble chitosan, 0.95 g (GlcN)2, 1.43 g (GlcN)3, and 1.18 g (GlcN)4 were recovered.
高星爱,(GAO, Xing’-Ai) 영남퇴계학연구원 2018 퇴계학논집 Vol.- No.23
1. 引言——关于现代新儒学 2. 对西方哲学的研究 3. 对传统文化的看法 4. 融会东西方文化 5. 结论 하린과 박종홍은 동시대를 살았던 사람이다. 국가와 민족의 위기 상황을 해결하기 위하여 서양철학의 연구로부터 유익한 경험과 교훈을 얻고자 시도하였다. 그들은 서양문화의 충격에 직면하여 서양문화를 흡수함으로써 전통문화를 재해석하여야 함을 강하게 주장하였다. 또한 동서양의 사상문화에 대한 융합을 자신의 사명으로 받아들이고 융합에 이르기 위한 세 가지 절차——전통문화에 대한 전승, 서양문화에 대한 흡수, 동서양 문화에 대한 융합을 통하여 궁극적으로 사상의 발전과 창조를 이루어내고자 하였던 학문적 시도는 거의 비슷하다. 하지만 현대 신유학의 발전을 놓고 보면, 형성과 발전의 단계를 거쳐 하린의 동서양 문화 및 철학에 대한 관점은 비교적 객관적이고 이성적인 반면에 박종홍은 서양철학 제1세대로서 동서양 문화의 장, 단점과 우열 비교에 주목하고 있다.
Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Wu, Hai-Yan,Wang, Li-Chun,Gao, Xing-Ai,Jin, Rong-De,Fan, Zuo-Wei,Kim, Kil-Yong,Zhao, Lan-Po Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg $L^{-1}$ after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg $ha^{-1}$) soil inoculation with Burkholderia sp. (Twenty milliliters/plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
오인재 ( In Jae Oh ),고성애 ( Xing Ai Gao ),조경현 ( Gyung Hyun Jo ),김영주 ( Young Ju Kim ),박노동 ( Ro Dong Park ) 한국키틴키토산학회 2009 한국키틴키토산학회지 Vol.14 No.2
In order to input chitin and chitosan into pharmaceutical, biotech, cosmetic, and dietary supplementation, the product consistency and safety will become important for consideration by regulatory authorities. To compare internationally the specification and regulation of chitosan-related products, data has been collected and analyzed in definition, application and function, physicochemical and biological quality criteria, analysis method, and regulatory authority.