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( Kang Li Xin ),( Xiao Mei Chen ),( Chao Zhai ),( Li Xin Ma ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
A gene, ClCDA, encoding chitin deacetylase from Colletotrichum lindemuthianum, was optimized according to the codon usage bias of Pichia pastoris and synthesized in vitro by overlap extension PCR. It was secretorily expressed in P. pastoris GS115 using the constitutive expression vector pHMB905A, The expression level reached the highest with 110 mg/l culture supernatant after 72 h of methanol induction, which comprised 77.27 U/mg chitin deacetylase activity, SDS-PAGE, mass spectrometry, and deglycosylation assays demonstrated that partial recombinant protein was glycosylated with an apparent molecular mass of 33kDa, The amino acid sequences of recombinant proteins were confirmed by mass spectrometry.
Jun Wei Wu,Yu Rong Xing,Yi Bo Wen,Tian Fang Li,Quan De Feng,Xiao Ping Shang,Yun Long Li,Jin Jin Feng,Xin Xin Wang,Rong Qun Zhai,Xiang Fei He,Tao Chen,Xin Jian Liu,Jian Guo Wen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.2
Purpose: To investigate the prevalence of spina bifida occulta (SBO) and its relationship with the presence of overactive bladder (OAB) in middle-aged and elderly people in China. Methods: A cross-sectional community-based survey was carried out at 7 communities in Zhengzhou City, China from December 15, 2013 to June 10, 2014, where residents aged over 40 years were randomly selected to participate. All of the participants underwent lumbosacral radiographic analysis and relevant laboratory tests. A questionnaire including basic information, past medical history and present illness, and the OAB symptom score was filled out by all participants. Chi-square tests and logistic regression were used for data analysis with a P-value of <0.05 denoting statistical significance. Results: A total of 1,061 subjects were qualified for the final statistical analysis (58.8±11.7 years; male, 471 [44.4%]; female, 590 [55.6%]). The overall prevalence of SBO was 15.1% (160 of 1,061): 18.3% (86 of 471) in men and 12.5% (74 of 590) in women. Among these subjects, 13.7% (145 of 1,061) had OAB: 13.2% (62 of 471) in men and 14.1% (83 of 590) in women. The results of logistic regression showed that age, SBO, history of cerebral infarction (HCI), and constipation were risk factors for OAB (P<0.05), while sex, history of childhood enuresis (HCE), body mass index (BMI), and diabetes mellitus (DM) were not (P>0.05). In men, age, SBO, and constipation were risk factors for OAB (P<0.05), while HCE, BMI, DM, HCI, and benign prostate hyperplasia were not (P>0.05). In women, age, SBO, and HCI were risk factors for OAB (P<0.05), while HCE, BMI, DM, vaginal delivery, and constipation were not (P>0.05). Conclusions: The prevalence of SBO is high and it is related to OAB in middle-aged and elderly people in China.
Zhai, Yan-Chun,Dong, Bin,Wei, Wen-Qiang,He, Yan,Li, Xin-Qing,Cormier, Robert T.,Wang, Wei,Liu, Fen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21
Background: Esophageal cancer is one of the most frequently occurring malignancies and the seventh leading cause of cancer-related deaths in the world. The esophageal squamous cell carcinoma (ESCC) is the most common histological type of esophageal cancer worldwide. Materials and Methods: Our goal in this study was to detect phospholipase A2 Group IIA (PLA2G2A) and cyclooxygenase-2 (COX-2) immuno-expression in ESCC in a high-risk population in China. Results: Positive expression of PLA2G2A protein was observed in 57.2% (166/290) of the cases, while COX-2 was found in 257 of 290 samples (88.6%), both PLA2G2A and COX-2 being expressed in 153 cases (52.8%), with a significant agreement (Kappa=0.091, p=0.031).Overexpression of PLA2G2A was significantly correlated with the depth of invasion (p=0.001). Co-expression of PLA2G2A and COX-2 not only significantly correlated with the depth of invasion (p=0.004) but also with TNM stage (p=0.04). Conclusions: Our results showed that in patients with ESCC, PLA2G2A overexpression and PLA2G2A co-expression with COX-2 is significantly correlated with advanced stage. The biological role and pathophysiologic regulation of PLA2G2A and COX-2 overexpression in ESCC deserve further investigation.
Frame Synchronization Algorithm for LDPC Coded Burst Systems
Xin Man,Haitao Zhai,Eryang Zhang 한국전자통신연구원 2015 ETRI Journal Vol.37 No.5
We present a frame synchronization algorithm for low-density parity-check (LDPC) coded burst transmissions, which combines a conventional pilots-assisted frame synchronization algorithm and a code-aided algorithm based on the mean magnitude of the soft outputs from the LDPC decoder. With moderate computational complexity, the proposed algorithm is more efficient in bandwidth than conventional pilots-assisted algorithms. When compared with other code-aided algorithms, the proposed algorithm offers a better trade-off between complexity and performance. Simulation results in the case of an 8-PSK system with (1944, 972) LDPC code show that the proposed algorithm can achieve a performance equivalent to that of the perfect frame synchronization, with a bandwidth efficiency loss of 0.06 dB due to the use of pilot symbols.
Xiang Zhai,Xin-yang Li,Yu-jing Wang,Ke-ru Qin,Jin-rui Hu,Mei-ning Li,Hai-long Wang,Rui Guo 대한내분비학회 2022 Endocrinology and metabolism Vol.37 No.3
Background: It is well-established that serum testosterone in men decreases with age, yet the underlying mechanism of this changeremains elusive. Methods: The expression patterns of Fancd2 opposite-strand (Fancd2os) in BALB/c male mice and testicular tissue derived celllines (GC-1, GC-2, TM3, and TM4) were assessed using real-time polymerase chain reaction (RT-PCR), Western blot and immunofluorescence. The Fancd2os-overexpressing or knockdown TM3 cells were constructed by infecting them with lentivirus particlesand were used to evaluated the function of Fancd2os. The testosterone production was measured using enzyme linked immunosorbent assay (ELISA) and the steroidogenic enzymes such as steroidogenic acute regulatory protein (StAR), P450 cholesterol sidechain cleavage (P450scc), and 3β-hydroxysteroid dehydrogenase (3β-HSD) were analysed using RT-PCR. The apoptosis of TM3cells induced by ultraviolet light or testicular tissues was detected using flow cytometry, Western blot or dUTP-biotin nick end labeling (TUNEL) assays. Pearson correlation analysis was used to assess the correlation between the Fancd2os expression and TUNELpositive staining in mouse testicular Leydig cells. Results: The Fancd2os protein was predominantly expressed in mouse testicular Leydig cells and its expression increased with age. Fancd2os overexpression inhibited testosterone levels in TM3 Leydig cells, whereas knockdown of Fancd2os elevated testosteroneproduction. Fancd2os overexpression downregulated the levels of StAR, P450scc and 3β-HSD, while Fancd2os knockdown reversed this effect. Fancd2os overexpression promoted ultraviolet light-induced apoptosis of TM3 cells. In contrast, Fancd2os knockdown restrained apoptosis in TM3 cells. In vivo assays revealed that higher Fancd2os levels and mouse age were associated with increased apoptosis in Leydig cells and decreased serum testosterone levels. Pearson correlation analysis exhibited a strong positivecorrelation between the expression of Fancd2os and TUNEL-positive staining in mouse testicular Leydig cells. Conclusion: Our findings suggest that Fancd2os regulates testosterone synthesis via both steroidogenic enzymes and the apoptoticpathway.
Zhongmin Liu,Liangzhu Cheng,Xin Zhai 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1
We studied the transient driving power of an oil pump for 1.6 L PFI engine in a passenger car under the new European driving cycle (NEDC) and worldwide harmonized lightduty test cycle (WLTC), and analyze the influence of control strategies on the energy-saving potential of the oil pump. To measure the dynamic matching conditions between the oil pump supplements and the engine lubrication requirements, a test system of the engine lubrication performance in passenger cars was built using the hardware-in-the loop (HiL) method. Subsequently, a set of acceleration and deceleration test cycles was designed to represent the harsh lubrication conditions in the standard driving cycles, so as to facilitate analysis of the reasons for driving energy loss of oil pump under transient conditions. Further, three kinds of control strategies of oil pumps used in engine design were analyzed and a comparison was given on their effects on the driving energy consumption and lubrication safety under NEDC and WLTC. The test results indicate that the hydraulic energy losses caused by the pressure relief valve opening and lubricant flow hysteresis were the main reasons for the driving energy increments in the oil pump under transient conditions. In addition, the variable lubrication flow control strategies were effective in reducing the pressure relief valve opening duration and hydraulic energy losses, yet had minimal influence on the hydraulic energy losses caused by lubricant flow hysteresis. Compared with the constant one, 2-stage variable control strategy reduced the overall driving energy by 42.7 % and 46 % in NEDC and WLTC, respectively, while the corresponding hydraulic energy output fulfilled the requirements of engine lubrication. By the 2-stage oil pump, the total fuel consumption of vehicle in WLTC was reduced by 1.93 % and 0.94 % at 25 °C and 100 °C, respectively.