A novel synthesis of hexagonal cylinder-like ZnO with an excellent performance by a surfactant-free microemulsion-hydrothermal method

Xiaoyu Zhang,Ying Han,Wenjiao Liu,Ning Pan,Dejie Li,Jinling Chai 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

A W/O surfactant-free microemulsion (SFME) template-hydrothermal method for the preparation of ZnOproducts was proposed. Using the SFME system containing hexane, propan-2-ol and water, hexagonalcylinder-like (twinning) ZnO microcrystals were synthesized. The XRD, SEM and TEM characterizationsshowed that the synthesized ZnO microcrystals have a hexagonal wurtzite structure. At hydrothermaltemperatures of 120–140 C, hexagonal cylinder-like ZnO microcrystals were synthesized, whereas thehexagonal cylinder twinning ZnO microcrystals were obtained at 160 C. The growth mechanism of theZnO microcrystals was also investigated. The EPR and XPS spectra of the synthesized ZnO products showed that there are oxygen vacancies onthe ZnO surface. The photodegradation behavior of methylene blue (MB, 10 ppm) with a UV-Hg lamp(250 W) as the light source in ZnO aqueous suspension (0.3 g L 1) was investigated. The ZnO productsynthesized at 140 ℃ can significantly photocatalyze the degradation of lower concentration MBsolutions. After 35 min of irradiation, the photocatalytic decomposition rate reached 98.3%. It is hopedthat this work could serve as an important stepping stone for establishing a novel hydrothermal methodbased on SFME system, therefore paving the way for other potential ideas on the application of SFMEsystems.

Effects of the Helicobacter pylori Virulence Factor CagA and Ammonium Ion on Mucins in AGS Cells

Xiaoyu Zhang,Ding Shi,Yong-pan Liu,Wu-jie Chen,Dong Wu 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.5

Purpose: To investigate the effects of Helicobacter pylori (H. pylori)-CagA and the urease metabolite NH4 + on mucin expression inAGS cells. Materials and Methods: AGS cells were transfected with CagA and/or treated with different concentrations of NH4CL. Mucingene and protein expression was assessed by qPCR and immunofluorescence assays, respectively. Results: CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin andMUC6 expression. MUC5AC, MUC6, and MUC2 expression in AGS cells increased with increasing NH4 + concentrations untilreaching a peak level at 15 mM. MUC5B mRNA expression in AGS cells (NH4 + concentration of 15 mM) was significantly higherthan that at 0, 5, and 10 mM NH4 + . No changes in E-cadherin expression in AGS cells treated with NH4 + were noted, except at 20mM. The expression of MUC5AC, MUC2, and MUC6 mRNA in CagA-transfected AGS cells at an NH4 + concentration of 15 mM wassignificantly higher than that at 0 mM, and decreased at higher concentrations. The expression of MUC5B mRNA increased withincreases in NH4 + concentration, and was significantly higher compared to that in untreated cells. No significant change in the expressionof E-cadherin mRNA in CagA-transfected AGS cells was observed. Immunofluorescence assays confirmed the observedchanges. Conclusion: H. pylori may affect the expression of MUC5AC, MUC2, MUC5B, and MUC6 in AGS cells via CagA and/or NH4 + , butnot E-cadherin.

Understanding Biochar Role in Soil Quality and Functioning: Where to Go?

Genxing Pan,Stephen Joseph,Lianqing Li,Xiaoyu Liu,Rongjun Bian,Jinwei Zheng 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Variation in the quantity and composition of phosphorus accumulating organisms in activated sludge driven by nitrate-nitrogen

Wang Xiaoling,Shi Chunyan,Pan Wenbo,Lu Hai,Zhang Xiaoyu 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.7

Anaerobic/anoxia sequencing batch reactor (A/ASBR) system was used to analyze the quantity and composition of each branch of phosphorus accumulating organisms (PAOs) in activated sludge under different nitrate-nitrogen (NO 3 − -N) concentrations by using real-time quantitative polymerase chain reaction (PCR) technology. The study determined whether NO 3 − -N and its concentration change were the main driving factors for the variation of the quantity and composition of each branch of PAOs. The results show that with the increase of NO 3 − -N concentration from 10 mg/L to 40 mg/L, the number of bacterial 16S rRNA genes in the A/ASBR reactor changed slightly at 6.81×1011–7.53×1011 copies/g dry sludge. The number of PAO genes (Acc 16S rRNA) increased from 1.98×1011 to 3.53×1011 copies/g dry sludge, and the total number of ppk1 genes increased from 1.25×1011 to 3.59×1011 copies/g dry sludge. Additionally, the number of polyphosphate kinase (ppk) genes in Accumulibacter branch IA, IIC and IID was high, and the changes were positively related to the concentration of NO 3 − -N, while the number of branches in IIA, IIB and IIF was very low. The dosing concentration of NO 3 − -N was the main driving factor for the change of PAOs and their branch number and composition in the A/ASBR reactor.

Porous NiS@Ni2P nanoframe as a multi-functional catalyst for enhanced oxygen evolution and urea oxidation reactions

Yin Huang,Yaoyao Pan,Xiaoyu Huang,Jialu Zhao,Xiuhua Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-

During water electrolysis, multifunctional electrocatalysts with outstanding functionality and endurancemust be thoughtfully constructed and designed. Below, we report a hollow NiS@Ni2P nanoframeheterostructure that works well as a substitute catalyst for the urea oxidation and general water splittingreactions. The hollow NiS@Ni2P nanoframe was synthesized by ion exchange, sulfurization and phosphating,which is favorable for achieving a heterostructure. The NiS@Ni2P catalyst exhibits excellent catalyticactivity and strong long-term stability in 1.0 M KOH solutions for the hydrogen evolution reaction, whichneeds 121 mV to achieve 10 mA cm2. And for the oxygen evolution reaction, the catalyst needs 311 mVto acquire 50 mA cm2. These advantages come from the optimal electronic structural configuration,hierarchical hollow nanoframe structure, and large surface area. It could attain 10 mA cm2 at 1.41 Vwhen utilized as a urea oxidation reaction anode, which is lower than the oxygen evolution reaction. For total water splitting and urea oxidation process, NiS@Ni2P as a bifunctional catalyst holds tremendouspromise due to its strong electrocatalytic activity, ease of manufacture, and low cost of raw ingredients.

CD44 Antibody-Conjugated Gold Nanostars as SERS Probes for Distinguishing Cancer Cells (A549 Cells, H1229 Cells) from Normal Cells (ATII Cells)

Hang Zhao,Xiaowei Cao,Man Wang,Lin Tao,Xiaoyu Pan,Chunwei Yuan,Weiping Qian 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.3

In this paper, we report a novel antibody conjugated gold nanostars (AuNSs) as surface-enhanced Raman spectroscopy (SERS) probes for distinguishing cancer cells (A549 cells, H1229 cells) from normal cells (ATII cells). In such a probe, bovine serum albumin (BSA) was served as the protective agent and stabilizing agent, 4-mercaptobenzoic acid (4-MBA) was used as the Raman reporter to generate SERS signals as well as the conjugation agent for attaching CD44 antibody (anti-CD44) molecules to AuNSs, where anti-CD44 could effectively target to CD44 protein overexpressed cells. All the results of the experiments con¯rmed that more SERS probes have been targeted to cancer cells (A549 cells and H1229 cells) than that of normal cells (ATII cells) under the same condition. The anti-CD44 SERS probes hold a potential application in distinguishing cancer cells from normal cells with high sensitivity and good biocompatibility.

Accelerated Time-of-Flight Magnetic Resonance Angiography with Sparse Undersampling and Iterative Reconstruction for the Evaluation of Intracranial Arteries

Hehan Tang,Na Hu,Yuan Yuan,Chunchao Xia,Xiumin Liu,Panli Zuo,Aurelien F. Stalder,Michaela Schmidt,Xiaoyue Zhou,Bin Song,Jiayu Sun 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.2

Objective: To compare the image quality of three-dimensional time-of-flight (TOF) magnetic resonance angiography (MRA) with sparse undersampling and iterative reconstruction (sparse TOF) with that of conventional TOF MRA. Materials and Methods: This study included 56 patients who had undergone sparse TOF MRA for intracranial artery evaluation on a 3T MR scanner. Conventional TOF MRA scans were also acquired from 29 patients with matched acquisition times and another 27 patients with matched scanning parameters. The image quality was scored using a five-point scale based on the delineation of arterial vessel segments, artifacts, overall vessel visualization, and overall image quality by two radiologists independently, and the data were analyzed using the non-parametric Wilcoxon signed-rank test. Contrast ratios (CRs) of vessels were compared using the paired t test. Interobserver agreement was calculated using the kappa test. Results: Compared with conventional TOF at the same spatial resolution, sparse TOF with an acceleration factor of 3.5 could reduce acquisition time by 40% and showed comparable image quality. In addition, when compared with conventional TOF with the same acquisition time, sparse TOF with an acceleration factor of 5 could also achieve higher spatial resolution, better delineation of vessel segments, fewer artifacts, higher image quality, and a higher CR (p < 0.05). Good-to-excellent interobserver agreement (к: 0.65–1.00) was obtained between the two radiologists. Conclusion: Compared with conventional TOF, sparse TOF can achieve equivalent image quality in a reduced duration. Furthermore, using the same acquisition time, sparse TOF could improve the delineation of vessels and decrease image artifacts.

Three-dimensional thermal-hydraulics/neutronics coupling analysis on the full-scale module of helium-cooled tritium-breeding blanket

Lian Qiang,Tang Simiao,Zhu Longxiang,Zhang Luteng,Sun Wan,Bu Shanshan,Pan Liangming,Tian Wenxi,Qiu Suizheng,Su G.H.,Wu Xinghua,Wang Xiaoyu 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.11

Blanket is of vital importance for engineering application of the fusion reactor. Nuclear heat deposition in materials is the main heat source in blanket structure. In this paper, the three-dimensional method for thermalhydraulics/ neutronics coupling analysis is developed and applied for the full-scale module of the helium-cooled ceramic breeder tritium breeding blanket (HCCB TBB) designed for China Fusion Engineering Test Reactor (CFETR). The explicit coupling scheme is used to support data transfer for coupling analysis based on cell-to-cell mapping method. The coupling algorithm is realized by the user-defined function compiled in Fluent. The threedimensional model is established, and then the coupling analysis is performed using the paralleled Coupling Analysis of Thermal-hydraulics and Neutronics Interface Code (CATNIC). The results reveal the relatively small influence of the coupling analysis compared to the traditional method using the radial fitting function of internal heat source. However, the coupling analysis method is quite important considering the nonuniform distribution of the neutron wall loading (NWL) along the poloidal direction. Finally, the structure optimization of the blanket is carried out using the coupling method to satisfy the thermal requirement of all materials. The nonlinear effect between thermal-hydraulics and neutronics is found during the blanket structure optimization, and the tritium production performance is slightly reduced after optimization. Such an adverse effect should be thoroughly evaluated in the future work.

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Zhao Jingyi,Li Bingyan,Ren Yongxia,Liang Tiansong,Wang Juan,Zhai Suna,Zhang Xiqian,Zhou Pengcheng,Zhang Xiangxian,Pan Yuanyuan,Gao Fangfang,Zhang Sulan,Li Liming,Yang Yongqiang,Deng Xiaoyu,Li Xiaole,C 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Compelling evidence has indicated the vital role of lysine-specific demethylase 4 A (KDM4A), hypoxia-inducible factor-1α (HIF1α) and the mechanistic target of rapamycin (mTOR) signaling pathway in nasopharyngeal carcinoma (NPC). Therefore, we aimed to investigate whether KDM4A affects NPC progression by regulating the HIF1α/DDIT4/mTOR signaling pathway. First, NPC and adjacent tissue samples were collected, and KDM4A protein expression was examined by immunohistochemistry. Then, the interactions among KDM4A, HIF1α and DDIT4 were assessed. Gain- and loss-of-function approaches were used to alter KDM4A, HIF1α and DDIT4 expression in NPC cells. The mechanism of KDM4A in NPC was evaluated both in vivo and in vitro via RT-qPCR, Western blot analysis, MTT assay, Transwell assay, flow cytometry and tumor formation experiments. KDM4A, HIF1α, and DDIT4 were highly expressed in NPC tissues and cells. Mechanistically, KDM4A inhibited the enrichment of histone H3 lysine 9 trimethylation (H3K9me3) in the HIF1α promoter region and thus inhibited the methylation of HIF1α to promote HIF1α expression, thus upregulating DDIT4 and activating the mTOR signaling pathway. Overexpression of KDM4A, HIF1α, or DDIT4 or activation of the mTOR signaling pathway promoted SUNE1 cell proliferation, migration, and invasion but inhibited apoptosis. KDM4A silencing blocked the mTOR signaling pathway by inhibiting the HIF1α/DDIT4 axis to inhibit the growth of SUNE1 cells in vivo. Collectively, KDM4A silencing could inhibit NPC progression by blocking the activation of the HIF1α/DDIT4/mTOR signaling pathway by increasing H3K9me3, highlighting a promising therapeutic target for NPC.