Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons

Huang, Qingxia,Lou, Tingting,Lu, Jing,Wang, Manying,Chen, Xuenan,Xue, Linyuan,Tang, Xiaolei,Qi, Wenxiu,Zhang, Zepeng,Su, Hang,Jin, Wenqi,Jing, Chenxu,Zhao, Daqing,Sun, Liwei,Li, Xiangyan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6

Background: Aerobic cellular respiration provides chemical energy, adenosine triphosphate (ATP), to maintain multiple cellular functions. Sirtuin 1 (SIRT1) can deacetylate peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) to promote mitochondrial biosynthesis. Targeting energy metabolism is a potential strategy for the prevention and treatment of various diseases, such as cardiac and neurological disorders. Ginsenosides, one of the major bioactive constituents of Panax ginseng, have been extensively used due to their diverse beneficial effects on healthy subjects and patients with different diseases. However, the underlying molecular mechanisms of total ginsenosides (GS) on energy metabolism remain unclear. Methods: In this study, oxygen consumption rate, ATP production, mitochondrial biosynthesis, glucose metabolism, and SIRT1-PGC-1α pathways in untreated and GS-treated different cells, fly, and mouse models were investigated. Results: GS pretreatment enhanced mitochondrial respiration capacity and ATP production in aerobic respiration-dominated cardiomyocytes and neurons, and promoted tricarboxylic acid metabolism in cardiomyocytes. Moreover, GS clearly enhanced NAD⁺-dependent SIRT1 activation to increase mitochondrial biosynthesis in cardiomyocytes and neurons, which was completely abrogated by nicotinamide. Importantly, ginsenoside monomers, such as Rg1, Re, Rf, Rb1, Rc, Rh1, Rb2, and Rb3, were found to activate SIRT1 and promote energy metabolism. Conclusion: This study may provide new insights into the extensive application of ginseng for cardiac and neurological protection in healthy subjects and patients.

Effect of Vacuum Thermal Annealing on a Molybdenum Bilayer Back Contact Deposited by Radio-Frequency Magnetron Sputtering for Chalcogenide- and Kesterite-Based Solar Cells

Xiaolei Liu,Hongtao Cui,Xiaojing Hao,Shujuan Huang,Gavin Conibeer 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.71 No.12

Molybdenum (Mo) thin films are still a dominant choice for the back contact layer of Cu(In,Ga)Se2 (CIGS) and Cu2ZnSnS4 (CZTS) solar cells. This paper presents a review of Mo back contacts for CIGS and CZTS solar cells, including the requirements for a good back contact, the reason for the choice of Mo, and post-treatment. Additionally, a Mo bilayer back contact was fabricated by varying the argon (Ar) pressure during sputtering to provide both low resistivity and good adhesion to the soda-lime glass substrate. The effects of vacuum thermal annealing on the electrical, morphological and structural properties of the Mo bilayer were also investigated. Vacuum thermal annealing was seen to densify the Mo bilayer, reduce the sheet resistance, and improve the bilayer’s adhesion to the soda-lime glass. The Mo bilayer back contact with a low sheet resistance of 0.132 / and strong adhesion was made for chalcogenide- and kesterite-based solar cells.

Structural Characterization and Thermal Behavior of a Novel Energetic Material: 1-Amino-1-(2,4-dinitrophenylhydrazinyl)-2,2-dinitroethylene

Xiaolei Ren,Xiangang Zuo,Kangzhen Xu,Yinghui Ren,Jie Huang,Jirong Song,Bozhou Wang,Fengqi Zhao 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel energetic material, 1-amino-1-(2,4-dinitrophenylhydrazinyl)-2,2-dinitroethylene (APHDNE), was synthesized by the reaction of 1,1-diamino-2,2-dinitroethylene (FOX-7) and 2,4-dinitrophenylhydrazine in Nmethyl pyrrolidone (NMP) at 110 ^oC. The theoretical investigation on APHDNE was curried out by B3LYP/6-311+G^* method. The IR frequencies analysis and NMR chemical shifts were performed and compared with the experimental results. The thermal behavior of APHDNE was studied by DSC and TG/DTG methods, and can be divided into two crystal phase transition processes and three exothermic decomposition processes. The enthalpy, apparent activation energy and pre-exponential factor of the first exothermic decomposition reaction were obtained as −525.3 kJ mol^−1, 276.85 kJ mol^−1 and 10^(26.22) s^−1, respectively. The critical temperature of thermal explosion of APHDNE is 237.7 ^oC. The specific heat capacity of APHDNE was determined with micro-DSC method and theoretical calculation method, and the molar heat capacity is 363.67 J mol^−1 K^−1 at 298.15 K. The adiabatic time-to-explosion of APHDNE was also calculated to be a certain value between 253.2-309.4 s. APHDNE has higher thermal stability than FOX-7.

HIGHLY CATALYTIC Ag/Co3O4 NANOCATALYSTS FABRICATED FOR RhB DYE DEGRADATION THROUGH A SIMPLE SILVER-MIRROR REACTION UNDER VISIBLE LIGHT

XIAOLEI SI,GUANGLIANG CHEN,Zhili Chen,JUN HUANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.8

In this paper, a highly catalytic and nanosized Ag/Co3O4 composite for rhodamine B (RhB) degradation was fabricated by using the co-precipitation method at room temperature. The Ag/Co3O4 structure and catalytic properties were characterized through scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer–Emmett–Teller (BET) gassorption measurements, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and UV-Vis spectroscopy. The results showed that the Co3O4 nanosheets prepared by hydrothermal synthesis mainly exposed (2 2 0) and (1 1 1) facets, which played an important role in determining its catalytic oxidation performance. The Co3O4 nanosheets doped with Ag nanoparticles by a simple silver-mirror reaction exhibited a stable and well-dispersed property in dye solution. Compared to the Ag and Co3O4 nanostructure, the Ag nanoparticles with bigger diameter (10 nm) on Co3O4 surface also readily produced surface-active oxygen species and exhibited a higher catalytic activity for the degradation of RhB solution (5 mg·L-1) under the visible light. The kinetic constant K of Ag/Co3O4 catalyst for RhB degradation reaction was evaluated to0.02724 min-1, which is relatively higher than those reported in the literatures.

Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression

Zhiqi Huang,Xingyu Li,Tian Xie,Changjiang Gu,Kan Ni,Qingqing Yin,Xiaolei Cao,Chunhui Zhang 대한암학회 2020 Cancer Research and Treatment Vol.52 No.4

Purpose RIOK1 has been proved to play an important role in cancer cell proliferation and migration in various types of cancers—such as colorectal and gastric cancers. However, the expression of RIOK1 in breast cancer (BC) and the relationship between RIOK1 expression and the development of BC are not well characterized. In this study, we assessed the expression of RIOK1 in BC and evaluated the mechanisms underlying its biological function in this disease context. Materials and Methods We used immunohistochemistry, western blot and quantitative real-time polymerase chain reaction to evaluate the expression of RIOK1 in BC patients. Then, knockdown or overexpression of RIOK1 were used to evaluate the effect on BC cells in vitro and in vivo. Finally, we predicted miR-204-5p could be a potential regulator of RIOK1. Results We found that the expression levels of RIOK1 were significantly higher in hormone receptor (HR)–negative BC patients and was associated with tumor grades (p=0.010) and p53 expression (p=0.008) and survival duration (p=0.011). Kaplan-Meier analysis suggested a tendency for the poor prognosis. In vitro, knockdown of RIOK1 could inhibit proliferation, invasion, and induced apoptosis in HR-negative BC cells and inhibited tumorigenesis in vivo, while overexpression of RIOK1 promoted HR-positive tumor progression. MiR-204-5p could regulate RIOK1 expression and be involved in BC progression. Conclusion These findings indicate that RIOK1 expression could be a biomarker of HR-negative BC, and it may serve as an effective prognostic indicator and promote BC progression.

Structural Characterization and Thermal Behavior of a Novel Energetic Material: 1-Amino-1-(2,4-dinitrophenylhydrazinyl)-2,2-dinitroethylene

Ren, Xiaolei,Zuo, Xiangang,Xu, Kangzhen,Ren, Yinghui,Huang, Jie,Song, Jirong,Wang, Bozhou,Zhao, Fengqi Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel energetic material, 1-amino-1-(2,4-dinitrophenylhydrazinyl)-2,2-dinitroethylene (APHDNE), was synthesized by the reaction of 1,1-diamino-2,2-dinitroethylene (FOX-7) and 2,4-dinitrophenylhydrazine in N-methyl pyrrolidone (NMP) at 110 $^{\circ}C$. The theoretical investigation on APHDNE was curried out by B3LYP/6-311+$G^*$ method. The IR frequencies analysis and NMR chemical shifts were performed and compared with the experimental results. The thermal behavior of APHDNE was studied by DSC and TG/DTG methods, and can be divided into two crystal phase transition processes and three exothermic decomposition processes. The enthalpy, apparent activation energy and pre-exponential factor of the first exothermic decomposition reaction were obtained as -525.3 kJ $mol^{-1}$, 276.85 kJ $mol^{-1}$ and $10^{26.22}s^{-1}$, respectively. The critical temperature of thermal explosion of APHDNE is 237.7 $^{\circ}C$. The specific heat capacity of APHDNE was determined with micro-DSC method and theoretical calculation method, and the molar heat capacity is 363.67 J $mol^{-1}K^{-1}$ at 298.15 K. The adiabatic time-to-explosion of APHDNE was also calculated to be a certain value between 253.2-309.4 s. APHDNE has higher thermal stability than FOX-7.

A Hierarchical Model for Mobile Ad Hoc Network Performability Assessment

( Shuo Zhang ),( Ning Huang ),( Xiaolei Sun ),( Yue Zhang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.8

Dynamic topology is one of the main influence factors on network performability. However, it was always ignored by the traditional network performability assessment methods when analyzing large-scale mobile ad hoc networks (MANETs) because of the state explosion problem. In this paper, we address this problem from the perspective of complex network. A two-layer hierarchical modeling approach is proposed for MANETs performability assessment, which can take both the dynamic topology and multi-state nodes into consideration. The lower level is described by Markov reward chains (MRC) to capture the multiple states of the nodes. The upper level is modeled as a small-world network to capture the characteristic path length based on different mobility and propagation models. The hierarchical model can promote the MRC of nodes into a state matrix of the whole network, which can avoid the state explosion in large-scale networks assessment from the perspective of complex network. Through the contrast experiments with OPNET simulation based on specific cases, the method proposed in this paper shows satisfactory performance on accuracy and efficiency.

FABRICATING WELL-DISPERSED NANOSIZED GOLD CATALYST ON TITANATE NANOWIRES SURFACE FOR 4-NITROPHENOL REDUCTION

GUANGLIANG CHEN,FEI XUE,ZHILI CHEN,XIAOLEI SI,JUN HUANG,XU ZHENG,SYLVAIN MASSEY 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3

In this paper, homogenous gold nanoparticles (AuNPs) with a high density and a narrow sizedistribution were successfully fabricated on titanate nanowires (TNWs) sca®olds in the absenceof organic capping agents. An ameliorated low-temperature hydrothermal method was used toprepare the TNWs sca®olds like bird's nest on the Ti substrate, and the nanowires diameter wasabout 30 – 80nm. Then, AuNPs were synthesized on the TNWs sca®olds with a deposition –precipitation urea (DPU) method. The TEM and XRD measurements indicated that well-crys-tallized face-centered cubic (fcc) AuNPs were homogeneously dispersed on TNWs, and AuNPswith average sizes of 2.7 nm and 4 nm were obtained, respectively for the theoretical gold loading5 wt.% and 8 wt.%. Inspiringly, the 8%-AuNPs/TNWs catalyst could reduce 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) within 210 s, and exhibited better catalytic reduction performancethan that of 5%-AuNPs/TNWs.

Genotypic Characteristics of Haemophilus influenzae Isolates from Pediatric Pneumonia Patients in Chengdu City, Sichuan, China

Tian Guozhong,Zhang Li,Li Machao,Wang Xiaolei,Zheng Yuhong,Li Xiaojing,Huang Cheng,Li Xuechun,Xie Yongqiong,Xu Li,Ren Hongyu,Shao Zhujun 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

Two hundred and seventy-three Haemophilus influenzae strains isolated from pediatric pneumonia patients in China were studied. We used Multilocus Sequence Typing (MLST) to analyze genotypic characteristics. All strains were biotyped and serotyped. Relatedness and patterns of genes among isolates were determined by the analysis of MLST and eBURST. H. influenzae primarily causes acute pneumonia in children under 1 year old. Nontypeable H. influenzae was responsible for most cases of pediatric pneumonia. All 273 strains were classified into eight biotypes. They mostly belonged to the I, II, and III biotypes (17.6%, 43.6%, and 22.7%, respectively). 62 strains (22.7%) produced β-lactamase. We found 28 novel alleles. Fifty different STs were found by MLST, of which 39 were novel. These were ST477 through ST508 and ST521 through ST527. Group 17 and predicted founders 503 were new groups in this study. No STs correlated with strains from Korea, which is adjacent to China. The H. influenzae strains from China appeared to have heterogeneous ST types patterns which may be the reason no outbreaks or epidemics of H. influenzae infections have occurred in Chengdu city, Sichuan, China.

Small molecule natural compound agonist of SIRT3 as a therapeutic target for the treatment of intervertebral disc degeneration

Jianle Wang,Majid Nisar,Chongan Huang,Xiangxiang Pan,Dongdong Lin,Gang Zheng,Haiming Jin,Deheng Chen,Naifeng Tian,Qianyu Huang,Yue Duan,Yingzhao Yan,Ke Wang,Congcong Wu,Jianing Hu,Xiaolei Zhang,Xiangy 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Oxidative stress-induced mitochondrial dysfunction is implicated in the pathogenesis of intervertebral disc degeneration (IVDD). Sirtuin 3 (SIRT3), a sirtuin family protein located in mitochondria, is essential for mitochondrial homeostasis; however, the role of SIRT3 in the process of IVDD has remained elusive. Here, we explored the expression of SIRT3 in IVDD in vivo and in vitro; we also explored the role of SIRT3 in senescence, apoptosis, and mitochondrial homeostasis under oxidative stress. We subsequently activated SIRT3 using honokiol to evaluate its therapeutic potential for IVDD. We assessed SIRT3 expression in degenerative nucleus pulposus (NP) tissues and oxidative stressinduced nucleus pulposus cells (NPCs). SIRT3 was knocked down by lentivirus and activated by honokiol to determine its role in oxidative stress-induced NPCs. The mechanism by which honokiol affected SIRT3 regulation was investigated in vitro, and the therapeutic potential of honokiol was assessed in vitro and in vivo. We found that the expression of SIRT3 decreased with IVDD, and SIRT3 knockdown reduced the tolerance of NPCs to oxidative stress. Honokiol (10 μM) improved the viability of NPCs under oxidative stress and promoted their properties of anti-oxidation, mitochondrial dynamics and mitophagy in a SIRT3-dependent manner. Furthermore, honokiol activated SIRT3 through the AMPKPGC- 1α signaling pathway. Moreover, honokiol treatment ameliorated IVDD in rats. Our study indicated that SIRT3 is involved in IVDD and showed the potential of the SIRT3 agonist honokiol for the treatment of IVDD.